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  1. Article ; Online: Understanding the link between neurotropic viruses, BBB permeability, and MS pathogenesis.

    Rani, Annu / Ergün, Süleyman / Karnati, Srikanth / Jha, Hem Chandra

    Journal of neurovirology

    2024  Volume 30, Issue 1, Page(s) 22–38

    Abstract: Neurotropic viruses can infiltrate the CNS by crossing the blood-brain barrier (BBB) through various mechanisms including paracellular, transcellular, and "Trojan horse" mechanisms during leukocyte diapedesis. These viruses belong to several families, ... ...

    Abstract Neurotropic viruses can infiltrate the CNS by crossing the blood-brain barrier (BBB) through various mechanisms including paracellular, transcellular, and "Trojan horse" mechanisms during leukocyte diapedesis. These viruses belong to several families, including retroviruses; human immunodeficiency virus type 1 (HIV-1), flaviviruses; Japanese encephalitis (JEV); and herpesviruses; herpes simplex virus type 1 (HSV-1), Epstein-Barr virus (EBV), and mouse adenovirus 1 (MAV-1). For entering the brain, viral proteins act upon the tight junctions (TJs) between the brain microvascular endothelial cells (BMECs). For instance, HIV-1 proteins, such as glycoprotein 120, Nef, Vpr, and Tat, disrupt the BBB and generate a neurotoxic effect. Recombinant-Tat triggers amendments in the BBB by decreasing expression of the TJ proteins such as claudin-1, claudin-5, and zona occludens-1 (ZO-1). Thus, the breaching of BBB has been reported in myriad of neurological diseases including multiple sclerosis (MS). Neurotropic viruses also exhibit molecular mimicry with several myelin sheath proteins, i.e., antibodies against EBV nuclear antigen 1 (EBNA1) aa411-426 cross-react with MBP and EBNA1 aa385-420 was found to be associated with MS risk haplotype HLA-DRB1*150. Notably, myelin protein epitopes (PLP
    MeSH term(s) Blood-Brain Barrier/virology ; Blood-Brain Barrier/metabolism ; Blood-Brain Barrier/pathology ; Humans ; Animals ; Multiple Sclerosis/virology ; Multiple Sclerosis/metabolism ; Multiple Sclerosis/pathology ; Mice ; Tight Junctions/virology ; Tight Junctions/metabolism ; Capillary Permeability ; Endothelial Cells/virology ; Endothelial Cells/metabolism ; Endothelial Cells/pathology
    Language English
    Publishing date 2024-01-08
    Publishing country United States
    Document type Journal Article ; Review ; Research Support, Non-U.S. Gov't
    ZDB-ID 1283265-0
    ISSN 1538-2443 ; 1355-0284
    ISSN (online) 1538-2443
    ISSN 1355-0284
    DOI 10.1007/s13365-023-01190-8
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    In stock of ZB MED Cologne/Königswinter

    Zs.A 4426: Show issues Location:
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  2. Article ; Online: Direct Stochastic Optical Reconstruction Microscopy (dSTORM) of Peroxisomes.

    Klein, Teresa / Sauer, Markus / Ergün, Süleyman / Karnati, Srikanth

    Methods in molecular biology (Clifton, N.J.)

    2023  Volume 2643, Page(s) 85–92

    Abstract: Peroxisomes are central metabolic organelles whose maturation and function depend on efficient and accurate targeting of peroxisomal membrane proteins (PMPs). Ultrastructural imaging of the PMPs is a quite difficult task as it requires high spatial and ... ...

    Abstract Peroxisomes are central metabolic organelles whose maturation and function depend on efficient and accurate targeting of peroxisomal membrane proteins (PMPs). Ultrastructural imaging of the PMPs is a quite difficult task as it requires high spatial and temporal resolution. Further, the spatial resolution of conventional light microscopy is limited due to the diffraction of light. However, recent methodological developments in super resolution microscopy showed us to access the nanoscale regimes spatially allowing to elucidate the membrane structures of cell organelles. In this chapter, we present protocols used in our laboratory for the super-resolution imaging of the peroxisomal membrane protein 14 (PEX14p) by direct stochastic optical reconstruction microscopy (dSTORM).
    MeSH term(s) Microscopy/methods ; Peroxisomes ; Intracellular Membranes ; Membrane Proteins
    Chemical Substances Membrane Proteins
    Language English
    Publishing date 2023-03-23
    Publishing country United States
    Document type Journal Article
    ISSN 1940-6029
    ISSN (online) 1940-6029
    DOI 10.1007/978-1-0716-3048-8_6
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  3. Book ; Online ; Thesis: Einfluss von D-β-Hydroxybutyrat auf Stoffwechsel und Interaktion mit Chemo-/Strahlentherapie bei triple negativen Mamma-Karzinom Zellen

    Winkler, Jana [Verfasser] / Kämmerer, Ulrike [Gutachter] / Ergün, Süleyman [Gutachter]

    2024  

    Author's details Jana Winkler ; Gutachter: Ulrike Kämmerer, Süleyman Ergün
    Keywords Medizin, Gesundheit ; Medicine, Health
    Subject code sg610
    Language German
    Publisher Universität Würzburg
    Publishing place Würzburg
    Document type Book ; Online ; Thesis
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  4. Article ; Online: The Impact of Oxygen Availability and Multilineage Communication on Organoid Maturation.

    Wörsdörfer, Philipp / Ergün, Süleyman

    Antioxidants & redox signaling

    2021  Volume 35, Issue 3, Page(s) 217–233

    Abstract: Significance: ...

    Abstract Significance:
    MeSH term(s) Age Factors ; Animals ; Biomarkers ; Cell Communication ; Cell Culture Techniques ; Cell Differentiation ; Cell Lineage/genetics ; Endothelium, Vascular/metabolism ; Epithelium/metabolism ; Humans ; Hypoxia/metabolism ; Organ Specificity ; Organogenesis ; Organoids/metabolism ; Oxygen/metabolism ; Stem Cell Niche ; Stem Cells/cytology ; Stem Cells/metabolism ; Tissue Engineering
    Chemical Substances Biomarkers ; Oxygen (S88TT14065)
    Language English
    Publishing date 2021-02-11
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't ; Review
    ZDB-ID 1483836-9
    ISSN 1557-7716 ; 1523-0864
    ISSN (online) 1557-7716
    ISSN 1523-0864
    DOI 10.1089/ars.2020.8195
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    In stock of ZB MED Cologne/Königswinter

    Zs.A 5488: Show issues Location:
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    Jg. 1995 - 2021: Lesesall (2.OG)
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  5. Book ; Online ; Thesis: Vaskularisierung von humanen neuralen Organoiden mit mesodermalen Progenitorzellen

    Kern, Anna [Verfasser] / Ergün, Süleyman [Gutachter]

    2022  

    Author's details Anna Kern ; Gutachter: Süleyman Ergün
    Keywords Medizin, Gesundheit ; Medicine, Health
    Subject code sg610
    Language German
    Publisher Universität Würzburg
    Publishing place Würzburg
    Document type Book ; Online ; Thesis
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  6. Book ; Online ; Thesis: Contribution of vascular adventitia-resident progenitor cells to new vessel formation in ex vivo 3D models

    Upcin, Berin [Verfasser] / Ergün, Süleyman [Gutachter]

    2022  

    Author's details Berin Upcin ; Gutachter: Süleyman Ergün
    Keywords Naturwissenschaften ; Science
    Subject code sg500
    Language English
    Publisher Universität Würzburg
    Publishing place Würzburg
    Document type Book ; Online ; Thesis
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  7. Article ; Online: Herpesviral interplay with peroxisome: An underexplored viral niche.

    Indari, Omkar / Ergün, Süleyman / Karnati, Srikanth / Chandra Jha, Hem

    Genes & diseases

    2023  Volume 10, Issue 4, Page(s) 1133–1135

    Abstract: The tendency of herpesvirus proteins, such as HCMV-vMIA and KSHV vFLIP, to interact with PEX19 and further interplay with MAVS is crucial. Investigating other herpesviral proteins that tend to interact with PEX19, and MAVS could provide an idea of ... ...

    Abstract The tendency of herpesvirus proteins, such as HCMV-vMIA and KSHV vFLIP, to interact with PEX19 and further interplay with MAVS is crucial. Investigating other herpesviral proteins that tend to interact with PEX19, and MAVS could provide an idea of whether this is a pan-herpesviral strategy.
    Language English
    Publishing date 2023-03-24
    Publishing country Netherlands
    Document type Journal Article
    ZDB-ID 2821806-1
    ISSN 2352-3042 ; 2352-3042
    ISSN (online) 2352-3042
    ISSN 2352-3042
    DOI 10.1016/j.gendis.2023.01.033
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  8. Article ; Online: Detection of Peroxisomal Proteins During Mycobacterial Infection.

    Behera, Ananyaashree / Biswas, Mainak / Ergün, Süleyman / Karnati, Srikanth / Sonawane, Avinash

    Methods in molecular biology (Clifton, N.J.)

    2023  Volume 2643, Page(s) 123–134

    Abstract: Peroxisomes are ubiquitous organelles with essential roles in lipid and reactive oxygen species (ROS) metabolism. They are involved in modulating the immune responses during microbial infection, thus having major impact on several bacterial and viral ... ...

    Abstract Peroxisomes are ubiquitous organelles with essential roles in lipid and reactive oxygen species (ROS) metabolism. They are involved in modulating the immune responses during microbial infection, thus having major impact on several bacterial and viral infectious diseases including tuberculosis. Intracellular pathogens such as Mycobacterium tuberculosis (M. tb) employ various strategies to suppress the host oxidative stress mechanisms to avoid killing by the host. Peroxisome-mediated ROS balance is crucial for innate immune responses to M. tb. Therefore, peroxisomes represent promising targets for host-directed therapeutics to tuberculosis. Here, we present protocols used in our laboratory for the culture of M. tb and detection of peroxisomal proteins in M. tb infected macrophages.
    MeSH term(s) Humans ; Reactive Oxygen Species/metabolism ; Tuberculosis ; Mycobacterium tuberculosis/metabolism ; Macrophages/metabolism ; Immunity, Innate
    Chemical Substances Reactive Oxygen Species
    Language English
    Publishing date 2023-03-23
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ISSN 1940-6029
    ISSN (online) 1940-6029
    DOI 10.1007/978-1-0716-3048-8_9
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  9. Article ; Online: Biogenesis and release of endothelial extracellular vesicles: Morphological aspects.

    Elsner, Clara / Ergün, Süleyman / Wagner, Nicole

    Annals of anatomy = Anatomischer Anzeiger : official organ of the Anatomische Gesellschaft

    2022  Volume 245, Page(s) 152006

    Abstract: Background: Cell-cell communication through extracellular vesicles (EVs) including exosomes, microvesicles and apoptotic bodies has been shown to be important in physiological homoeostasis as well as pathological processes such as atherosclerosis. ... ...

    Abstract Background: Cell-cell communication through extracellular vesicles (EVs) including exosomes, microvesicles and apoptotic bodies has been shown to be important in physiological homoeostasis as well as pathological processes such as atherosclerosis. However, while the cellular machinery controlling EV formation and composition has been studied during the past decade, less is known about the morphological process of their formation and release.
    Methods: Using different electron microscopic approaches including transmission-, scanning-, immun-, and serial block face electron microscopy we studied the morphogenetic events of EV formation and release. We analysed the different steps of EV formation and release in cultured myocardial endothelial (MyEnd) and aortic endothelial (AoEnd) cell lines under serum starvation and under inflammatory conditions.
    Results: We show that in a narrow time frame, the number of active cells and microvesicle (MV) producing cells increased in dependence of time spent in cultivation and additional stimulation by TNF-α. However, MV secretion was a highly heterogeneous process which couldn´t be seen in all cells cultivated under the same conditions. Release of MVs could be observed all over the cells' surface with no preferred release site. While no single specific microscopic approach applied was sufficient to provide a comprehensive analysis of EV biogenesis, we show that the limitations of one technique could be compensated by the qualities of the respective other applied techniques, thus enabling us to provide a detailed ultrastructural analysis of MV and exosome biogenesis. Surprisingly, exosome release in endothelial cells occurred via a yet undescribed process indicating that MVBs were incorporated into a novel distinct cellular compartment covered by fenestrated endothelium before exosome release. Lastly, we could show that TNF-α stimulation of AoEnd cells leads not only to the upregulation of CD44 in parental cells, but also to incorporation of CD44 into the membranes of generated MVs and exosomes.
    Conclusions: Taken together, our data contribute to a better understanding of biogenesis and release of EVs. We conclude that under inflammatory conditions, EVs can mediate the transfer of CD44 from endothelial cells to target cells at distant sites including vessel wall cells and this could be a mechanism by which MVs may change the and thus contribute to the development and progression of atherosclerotic lesions.
    MeSH term(s) Humans ; Endothelial Cells ; Tumor Necrosis Factor-alpha/analysis ; Tumor Necrosis Factor-alpha/metabolism ; Extracellular Vesicles/metabolism ; Exosomes/chemistry ; Exosomes/metabolism ; Exosomes/ultrastructure ; Atherosclerosis ; Endothelium
    Chemical Substances Tumor Necrosis Factor-alpha
    Language English
    Publishing date 2022-09-29
    Publishing country Germany
    Document type Journal Article
    ZDB-ID 1106738-x
    ISSN 1618-0402 ; 0940-9602
    ISSN (online) 1618-0402
    ISSN 0940-9602
    DOI 10.1016/j.aanat.2022.152006
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    Ub I Zs.55: Show issues Location:
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  10. Book ; Online ; Thesis: Effekt von β-Hydroxybutyrat und Acetoacetat auf die Proliferationsaktivität und die Strahlensensibilität von Kolonkarzinomzellen mit unterschiedlichem p53-Status

    Kristen, Alexander Kurt [Verfasser] / Kämmerer, Ulrike [Gutachter] / Ergün, Süleyman [Gutachter]

    2023  

    Author's details Alexander Kurt Kristen ; Gutachter: Ulrike Kämmerer, Süleyman Ergün
    Keywords Medizin, Gesundheit ; Medicine, Health
    Subject code sg610
    Language German
    Publisher Universität Würzburg
    Publishing place Würzburg
    Document type Book ; Online ; Thesis
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