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  1. Article: Evaluation of the Use of Singleplex and Duplex CerTest VIASURE Real-Time PCR Assays to Detect Common Intestinal Protist Parasites.

    Dashti, Alejandro / Alonso, Henar / Escolar-Miñana, Cristina / Köster, Pamela C / Bailo, Begoña / Carmena, David / González-Barrio, David

    Diagnostics (Basel, Switzerland)

    2024  Volume 14, Issue 3

    Abstract: ... ...

    Abstract Cryptosporidium
    Language English
    Publishing date 2024-02-01
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2662336-5
    ISSN 2075-4418
    ISSN 2075-4418
    DOI 10.3390/diagnostics14030319
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article: Retrospective Study for the Clinical Evaluation of a Real-Time PCR Assay with Lyophilized and Ready-to-Use Reagents for

    Peris, María Paz / Martín-Saco, Gloria / Alonso-Ezcurra, Henar / Escolar-Miñana, Cristina / Rezusta, Antonio / Acero, Raquel / Milagro-Beamonte, Ana

    Diagnostics (Basel, Switzerland)

    2022  Volume 12, Issue 9

    Abstract: Streptococcus agalactiae is a leading cause of sepsis and meningitis in newborns and young infants. Screening programs and intrapartum antibiotic prophylaxis have reduced early neonatal onset of disease. The aim of this study was to evaluate a molecular ... ...

    Abstract Streptococcus agalactiae is a leading cause of sepsis and meningitis in newborns and young infants. Screening programs and intrapartum antibiotic prophylaxis have reduced early neonatal onset of disease. The aim of this study was to evaluate a molecular assay with lyophilized and ready-to-use reagents: VIASURE® Streptococcus B Real Time PCR detection kit (CerTest Biotec) (Viasure qPCR assay) compared to both the GBS culture and a molecular assay with separated and frozen reagents: Strep B Real-TM Quant (Sacace Biotecnologies®) (Sacace qPCR assay). A total of 413 vaginal−rectal swabs from women between the 35th and 37th weeks of pregnancy were processed. GBS culture was firstly achieved through Granada medium and Columbia CNA agar at 35 °C in aerobic conditions. Then, nucleic acid extraction was performed for subsequent molecular analysis using both commercial assays. Discordant results were resolved via bidirectional Sanger sequencing. Viasure qPCR assay clinical sensitivity was 0.97 (0.92−0.99) and specificity 1 (0.98−1). This retrospective study demonstrated the good clinical parameters and the strong overall agreement (99.3%) between the Viasure qPCR assay and both reference assays. Finally, the added value observed of the assay under study was the stabilized and ready-to-use format, reducing the number of time-consuming steps, permitting the storage at room temperature, facilitating transport, being environmentally respectful, and reducing additional costs.
    Language English
    Publishing date 2022-09-09
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2662336-5
    ISSN 2075-4418
    ISSN 2075-4418
    DOI 10.3390/diagnostics12092189
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

  3. Article ; Online: Evaluation of a Novel Commercial Real-Time PCR Assay for the Simultaneous Detection of

    Dashti, Alejandro / Alonso, Henar / Escolar-Miñana, Cristina / Köster, Pamela C / Bailo, Begoña / Carmena, David / González-Barrio, David

    Microbiology spectrum

    2022  Volume 10, Issue 3, Page(s) e0053122

    Abstract: ... ...

    Abstract Cryptosporidium
    MeSH term(s) Cryptosporidiosis/diagnosis ; Cryptosporidiosis/parasitology ; Cryptosporidium/genetics ; Cryptosporidium parvum/genetics ; Diarrhea/diagnosis ; Entamoeba histolytica/genetics ; Feces ; Giardia lamblia/genetics ; Giardiasis/diagnosis ; Giardiasis/parasitology ; Humans ; Real-Time Polymerase Chain Reaction
    Language English
    Publishing date 2022-05-03
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2807133-5
    ISSN 2165-0497 ; 2165-0497
    ISSN (online) 2165-0497
    ISSN 2165-0497
    DOI 10.1128/spectrum.00531-22
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

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