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  1. Article ; Online: Reduced ability to neutralize the Omicron variant among adults after infection and complete vaccination with BNT162b2, ChAdOx1, or CoronaVac and heterologous boosting.

    Espíndola, Otávio Melo / Fuller, Trevon L / de Araújo, Mia Ferreira / Tort, Luis Fernando Lopez / Guaraldo, Lusiele / Calvet, Guilherme / Resende, Paola / Bonaldo, Myrna / Whitworth, Jimmy / Smith, Chris / Siqueira, Marilda / Brasil, Patrícia

    Scientific reports

    2023  Volume 13, Issue 1, Page(s) 7437

    Abstract: COVID-19 vaccines have dramatically reduced rates of severe infection requiring hospitalization. However, SARS-CoV-2 variants have reduced vaccine effectiveness at preventing any symptomatic infection. This real-world study analyzed binding and ... ...

    Abstract COVID-19 vaccines have dramatically reduced rates of severe infection requiring hospitalization. However, SARS-CoV-2 variants have reduced vaccine effectiveness at preventing any symptomatic infection. This real-world study analyzed binding and neutralizing antibodies generated after complete vaccination and boosting across three vaccine platforms. Binding antibodies decayed most slowly in people under 60 with hybrid immunity. Neutralizing antibodies against Omicron BA.1 were reduced compared to other variants. The anamnestic anti-spike IgG response to the first boost was more pronounced than after the second boost. Monitoring of the effects of SARS-CoV-2 mutations on disease severity and the effectiveness of therapeutics is warranted.
    MeSH term(s) Adult ; Humans ; COVID-19 Vaccines ; BNT162 Vaccine ; COVID-19/prevention & control ; SARS-CoV-2/genetics ; Vaccination ; Antibodies, Neutralizing ; Antibodies, Viral
    Chemical Substances sinovac COVID-19 vaccine ; COVID-19 Vaccines ; BNT162 Vaccine ; Antibodies, Neutralizing ; Antibodies, Viral
    Language English
    Publishing date 2023-05-08
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2615211-3
    ISSN 2045-2322 ; 2045-2322
    ISSN (online) 2045-2322
    ISSN 2045-2322
    DOI 10.1038/s41598-023-34035-9
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: PCR-based diagnosis is not always useful in the acute acquired toxoplasmosis in immunocompetent individuals.

    Neves, Elizabeth Souza / Espíndola, Otavio Melo / Curi, André / Amendoeira, Maria Regina / Rocha, Danielle Nascimento / Gomes, Leonardo Henrique Ferreira / Guida, Letícia Cunha

    Parasitology research

    2021  Volume 120, Issue 2, Page(s) 763–767

    Abstract: Toxoplasmosis is the most prevalent zoonosis in the world and is associated with a large spectrum of diseases. Acute acquired toxoplasmosis (AAT) is considered a benign and self-limiting disease but severe postnatal infections have been reported, ... ...

    Abstract Toxoplasmosis is the most prevalent zoonosis in the world and is associated with a large spectrum of diseases. Acute acquired toxoplasmosis (AAT) is considered a benign and self-limiting disease but severe postnatal infections have been reported, particularly in South America. Laboratory diagnosis is based on the detection of anti-Toxoplasma gondii IgM, IgG, and presence of low IgG avidity. However, these assays present limitations, and therefore, PCR has been suggested as an alternative diagnostic tool. In this study, we performed real-time and nested PCR in DNA blood samples from 59 individuals with AAT lasting less than 80 days. None of the patients had parasitic DNA detected by PCR, even in the more severe cases or when blood was collected early after disease onset. These negative results indicate that the parasitemia kinetics needs investigation to determine the best time for blood sampling, especially in immunocompetent individuals. Thus, we emphasize that a negative PCR result does not exclude recent T. gondii infection, and serological criteria are still decisive for the laboratory diagnosis of AAT.
    MeSH term(s) Acute Disease ; Adolescent ; Adult ; Child ; DNA, Protozoan/blood ; DNA, Protozoan/genetics ; Female ; Humans ; Middle Aged ; Molecular Diagnostic Techniques ; Negative Results ; Polymerase Chain Reaction ; Toxoplasma/genetics ; Toxoplasma/isolation & purification ; Toxoplasmosis/blood ; Toxoplasmosis/diagnosis ; Toxoplasmosis/parasitology ; Young Adult
    Chemical Substances DNA, Protozoan
    Language English
    Publishing date 2021-01-08
    Publishing country Germany
    Document type Journal Article
    ZDB-ID 284966-5
    ISSN 1432-1955 ; 0932-0113 ; 0044-3255
    ISSN (online) 1432-1955
    ISSN 0932-0113 ; 0044-3255
    DOI 10.1007/s00436-020-07022-6
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Uh II Zs.124: Show issues Location:
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    bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular
    ab Jg. 2022: Lesesaal (EG)

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  3. Article ; Online: Chitotriosidase 1 in the cerebrospinal fluid as a putative biomarker for HTLV-1-associated myelopathy/tropical spastic paraparesis (HAM/TSP) progression.

    Gomes, Yago Côrtes Pinheiro / Freitas, Nicole Lardini / Souza, Flávia Santos / Sandim, Vanessa / Pereira, Denise Abreu / Nogueira, Fábio César Sousa / Echevarria-Lima, Juliana / Leite, Ana Claudia Celestino Bezerra / Lima, Marco Antonio Sales Dantas / Silva, Marcus Tulius Teixeira / Araújo, Abelardo Queiroz Campos / Vicente, Ana Carolina Paulo / Espíndola, Otávio Melo

    Frontiers in immunology

    2022  Volume 13, Page(s) 949516

    Abstract: Human T-lymphotropic virus type 1 (HTLV-1)-associated myelopathy/tropical spastic paraparesis (HAM/TSP) is an inflammatory neurodegenerative disease that affects motor, urinary, intestinal, and sensory functions. Typically, HAM/TSP is slowly progressive, ...

    Abstract Human T-lymphotropic virus type 1 (HTLV-1)-associated myelopathy/tropical spastic paraparesis (HAM/TSP) is an inflammatory neurodegenerative disease that affects motor, urinary, intestinal, and sensory functions. Typically, HAM/TSP is slowly progressive, but it may vary from limited motor disability after decades (very slow progression) to loss of motor function in a few years from disease onset (rapid). In this study, we aimed to identify prognostic biomarkers for HAM/TSP to support patient management. Thus, proteomic analysis of the cerebrospinal fluid (CSF) was performed with samples from HTLV-1 asymptomatic carriers (AC) (n=13) and HAM/TSP patients (n=21) with rapid, typical, and very slow progression using quantitative label-free liquid chromatography/tandem mass spectrometry. Enrichment analyses were also carried out to identify key biological processes associated with distinct neurological conditions in HTLV-1 infection. Candidate biomarkers were validated by ELISA in paired CSF and serum samples, and samples from HTLV-1-seronegative individuals (n=9) were used as controls. CSF analysis identified 602 proteins. Leukocyte/cell activation, immune response processes and neurodegeneration pathways were enriched in rapid progressors. Conversely, HTLV-1 AC and HAM/TSP patients with typical and very slow progression had enriched processes for nervous system development. Differential expression analysis showed that soluble vascular cell adhesion molecule 1 (sVCAM-1), chitotriosidase 1 (CHIT1), and cathepsin C (CTSC) were upregulated in HAM/TSP. However, only CHIT1 was significantly elevated after validation, particularly in HAM/TSP rapid progressors. In contrast, none of these biomarkers were altered in serum. Additionally, CSF CHIT1 levels in HAM/TSP patients positively correlated with the speed of HAM/TSP progression, defined as points in the IPEC-2 HAM/TSP disability scale per year of disease, and with CSF levels of phosphorylated neurofilament heavy chain, neopterin, CXCL5, CXCL10, and CXCL11. In conclusion, higher CSF levels of CHIT1 were associated with HAM/TSP rapid progression and correlated with other biomarkers of neuroinflammation and neurodegeneration. Therefore, we propose CHIT1 as an additional or alternative CSF biomarker to identify HAM/TSP patients with a worse prognosis.
    MeSH term(s) Biomarkers ; Disabled Persons ; Hexosaminidases ; Human T-lymphotropic virus 1 ; Humans ; Motor Disorders ; Neurodegenerative Diseases ; Paraparesis, Tropical Spastic/diagnosis ; Proteomics
    Chemical Substances Biomarkers ; Hexosaminidases (EC 3.2.1.-) ; chitotriosidase (EC 3.2.1.-)
    Language English
    Publishing date 2022-08-16
    Publishing country Switzerland
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2606827-8
    ISSN 1664-3224 ; 1664-3224
    ISSN (online) 1664-3224
    ISSN 1664-3224
    DOI 10.3389/fimmu.2022.949516
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article ; Online: Cerebrospinal fluid findings in neurological diseases associated with COVID-19 and insights into mechanisms of disease development.

    Espíndola, Otávio Melo / Brandão, Carlos Otávio / Gomes, Yago Côrtes Pinheiro / Siqueira, Marilda / Soares, Cristiane Nascimento / Lima, Marco Antônio Sales Dantas / Leite, Ana Claudia Celestino Bezerra / Torezani, Guilherme / Araujo, Abelardo Queiroz Campos / Silva, Marcus Tulius Teixeira

    International journal of infectious diseases : IJID : official publication of the International Society for Infectious Diseases

    2020  Volume 102, Page(s) 155–162

    Abstract: Objectives: To analyze the cerebrospinal fluid (CSF) of patients with SARS-CoV-2 infection and neurological manifestations to provide evidence for the understanding of mechanisms associated with central nervous system (CNS) involvement in COVID-19.: ... ...

    Abstract Objectives: To analyze the cerebrospinal fluid (CSF) of patients with SARS-CoV-2 infection and neurological manifestations to provide evidence for the understanding of mechanisms associated with central nervous system (CNS) involvement in COVID-19.
    Methods: Patients (n = 58) were grouped according to their main neurological presentation: headache (n = 14); encephalopathy (n = 24); inflammatory neurological diseases, including meningoencephalitis (n = 4), acute myelitis (n = 3), meningitis (n = 2), acute disseminated encephalomyelitis (ADEM) (n = 2), encephalitis (n = 2), and neuromyelitis optica (n = 1); and Guillain-Barré syndrome (n = 6). Data regarding age, sex, cerebrovascular disease, and intracranial pressure were evaluated in combination with CSF profiles defined by cell counts, total protein and glucose levels, concentration of total Tau and neurofilament light chain (NfL) proteins, oligoclonal band patterns, and detection of SARS-CoV-2 RNA.
    Results: CSF of patients with inflammatory neurological diseases was characterized by pleocytosis and elevated total protein and NfL levels. Patients with encephalopathy were mostly older men (mean age of 61.0 ± 17.6 years) with evidence of cerebrovascular disease. SARS-CoV-2 RNA in CSF was detected in 2 of 58 cases: a patient with refractory headache, and another patient who developed ADEM four days after onset of COVID-19 symptoms. Three patients presented intrathecal IgG synthesis, and four had identical oligoclonal bands in CSF and serum, indicating systemic inflammation.
    Conclusion: Patients with neurological manifestations associated with COVID-19 had diverse CSF profiles, even within the same clinical condition. Our findings indicate a possible contribution of viral replication on triggering CNS infiltration by immune cells and the subsequent inflammation promoting neuronal injury.
    MeSH term(s) Adult ; Aged ; COVID-19/cerebrospinal fluid ; COVID-19/complications ; Female ; Humans ; Inflammation/diagnosis ; Male ; Middle Aged ; Nervous System Diseases/cerebrospinal fluid ; Nervous System Diseases/etiology ; SARS-CoV-2
    Keywords covid19
    Language English
    Publishing date 2020-10-28
    Publishing country Canada
    Document type Journal Article
    ZDB-ID 1331197-9
    ISSN 1878-3511 ; 1201-9712
    ISSN (online) 1878-3511
    ISSN 1201-9712
    DOI 10.1016/j.ijid.2020.10.044
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    Zs.A 4516: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (2.OG)
    ab Jg. 2022: Lesesaal (EG)

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  5. Article ; Online: Following the Clues: Usefulness of Biomarkers of Neuroinflammation and Neurodegeneration in the Investigation of HTLV-1-Associated Myelopathy Progression.

    Souza, Flávia Dos Santos / Freitas, Nicole Lardini / Gomes, Yago Côrtes Pinheiro / Torres, Rafael Carvalho / Echevarria-Lima, Juliana / da Silva-Filho, Isaac Lima / Leite, Ana Claudia Celestino Bezerra / de Lima, Marco Antonio Sales Dantas / da Silva, Marcus Tulius Teixeira / Araújo, Abelardo de Queiroz Campos / Espíndola, Otávio Melo

    Frontiers in immunology

    2021  Volume 12, Page(s) 737941

    Abstract: Human T-lymphotropic virus type 1 (HTLV-1)-associated myelopathy/tropical spastic paraparesis (HAM/TSP) is a neurodegenerative disease due to axonal damage of the corticospinal secondary to an inflammatory response against infected T-cells. In the ... ...

    Abstract Human T-lymphotropic virus type 1 (HTLV-1)-associated myelopathy/tropical spastic paraparesis (HAM/TSP) is a neurodegenerative disease due to axonal damage of the corticospinal secondary to an inflammatory response against infected T-cells. In the present work, we aimed to evaluate biomarkers of neurodegeneration and neuroinflammation in the definition of HAM/TSP prognosis. Neurofilament light (NfL) and phosphorylated heavy (pNfH) chains, total Tau protein, cellular prion protein (PrPc), inflammatory chemokines, and neopterin were quantified in paired cerebrospinal fluid (CSF) and serum samples from HAM/TSP patients (n=21), HTLV-1 asymptomatic carriers (AC) (n=13), and HTLV-1 seronegative individuals with non-inflammatory non-degenerative neurological disease (normal-pressure hydrocephalus) (n=9) as a control group. HTLV-1 proviral load in peripheral blood mononuclear cells and the expression of chemokine receptors CCR4, CCR5, and CXCR3 in infected CD4
    MeSH term(s) Adult ; Aged ; Biomarkers/blood ; Biomarkers/cerebrospinal fluid ; Case-Control Studies ; Cross-Sectional Studies ; Cytokines/blood ; Cytokines/cerebrospinal fluid ; Disease Progression ; Female ; Host-Pathogen Interactions ; Human T-lymphotropic virus 1/pathogenicity ; Humans ; Inflammation Mediators/blood ; Inflammation Mediators/cerebrospinal fluid ; Male ; Middle Aged ; Neopterin/blood ; Neopterin/cerebrospinal fluid ; Nerve Degeneration ; Nerve Tissue Proteins/blood ; Nerve Tissue Proteins/cerebrospinal fluid ; Neurodegenerative Diseases/blood ; Neurodegenerative Diseases/cerebrospinal fluid ; Neurodegenerative Diseases/diagnosis ; Neurodegenerative Diseases/virology ; Paraparesis, Tropical Spastic/blood ; Paraparesis, Tropical Spastic/cerebrospinal fluid ; Paraparesis, Tropical Spastic/diagnosis ; Paraparesis, Tropical Spastic/virology ; Predictive Value of Tests ; Prognosis
    Chemical Substances Biomarkers ; Cytokines ; Inflammation Mediators ; Nerve Tissue Proteins ; Neopterin (670-65-5)
    Language English
    Publishing date 2021-10-26
    Publishing country Switzerland
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2606827-8
    ISSN 1664-3224 ; 1664-3224
    ISSN (online) 1664-3224
    ISSN 1664-3224
    DOI 10.3389/fimmu.2021.737941
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  6. Article: Cerebrospinal fluid findings in neurological diseases associated with COVID-19 and insights into mechanisms of disease development

    Espíndola, Otávio Melo / Brandão, Carlos Otávio / Gomes, Yago Côrtes Pinheiro / Siqueira, Marilda / Soares, Cristiane Nascimento / Lima, Marco Antônio Sales Dantas / Leite, Ana Claudia Celestino Bezerra / Torezani, Guilherme / Araujo, Abelardo Queiroz Campos / Silva, Marcus Tulius Teixeira

    Int. j. infect. dis

    Abstract: OBJECTIVES: To analyze the cerebrospinal fluid (CSF) of patients with SARS-CoV-2 infection and neurological manifestations to provide evidence for the understanding of mechanisms associated with central nervous system (CNS) involvement in COVID-19. ... ...

    Abstract OBJECTIVES: To analyze the cerebrospinal fluid (CSF) of patients with SARS-CoV-2 infection and neurological manifestations to provide evidence for the understanding of mechanisms associated with central nervous system (CNS) involvement in COVID-19. METHODS: Patients (n = 58) were grouped according to their main neurological presentation: headache (n = 14); encephalopathy (n = 24); inflammatory neurological diseases, including meningoencephalitis (n = 4), acute myelitis (n = 3), meningitis (n = 2), acute disseminated encephalomyelitis (ADEM) (n = 2), encephalitis (n = 2), and neuromyelitis optica (n = 1); and Guillain-Barré syndrome (n = 6). Data about age, sex, cerebrovascular disease, and intracranial pressure were evaluated in combination with CSF profiles defined by cell counts, total protein and glucose levels, concentration of total Tau and neurofilament light chain (NfL) proteins, oligoclonal band patterns, and detection of SARS-CoV-2 RNA. RESULTS: CSF of patients with inflammatory neurological diseases was characterized by pleocytosis and elevated total protein and NfL levels. Patients with encephalopathy were mostly older men (mean age of 61.0 ± 17.6 years) with evidence of cerebrovascular disease. SARS-CoV-2 RNA in CSF was detected in 2 of 58 cases: a patient with refractory headache, and another who developed ADEM four days after COVID-19 symptoms onset. Three patients presented intrathecal IgG synthesis, and four had identical oligoclonal bands in CSF and serum, indicating systemic inflammation. CONCLUSION: Patients with neurological manifestations associated with COVID-19 had diverse CSF profiles, even within the same clinical condition. Our findings indicate a possible contribution of viral replication on triggering CNS infiltration by immune cells and the inflammation promoting neuronal injury.
    Keywords covid19
    Publisher WHO
    Document type Article
    Note WHO #Covidence: #893929
    Database COVID19

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  7. Article ; Online: Cerebrospinal fluid findings in neurological diseases associated with COVID-19 and insights into mechanisms of disease development

    Espíndola, Otávio Melo / Brandão, Carlos Otávio / Gomes, Yago Côrtes Pinheiro / Siqueira, Marilda / Soares, Cristiane Nascimento / Lima, Marco Antônio Sales Dantas / Leite, Ana Claudia Celestino Bezerra / Torezani, Guilherme / Araujo, Abelardo Queiroz Campos / Silva, Marcus Tulius Teixeira

    International Journal of Infectious Diseases ; ISSN 1201-9712

    2020  

    Keywords Microbiology (medical) ; Infectious Diseases ; General Medicine ; covid19
    Language English
    Publisher Elsevier BV
    Publishing country us
    Document type Article ; Online
    DOI 10.1016/j.ijid.2020.10.044
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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