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Article ; Online: Development of a Novel Multiplex Immunoassay Multi-cruzi for the Serological Confirmation of Chagas Disease.

Elodie Granjon / Marie-Laure Dichtel-Danjoy / Esber Saba / Ester Sabino / Lea Campos de Oliveira / Maan Zrein

PLoS Neglected Tropical Diseases, Vol 10, Iss 4, p e

2016 Volume 0004596

Abstract: BACKGROUND:Chagas disease is due to the parasite Trypanosoma cruzi, a protist disseminated by a Triatome vector. This disease is endemic to Latin America and considered by WHO as one of the 17 world's neglected diseases. In Europe and in North America, ... ...

Abstract	BACKGROUND:Chagas disease is due to the parasite Trypanosoma cruzi, a protist disseminated by a Triatome vector. This disease is endemic to Latin America and considered by WHO as one of the 17 world's neglected diseases. In Europe and in North America, imported cases are also detected, due to migration of population outside of the endemic region. Diagnosis of T. cruzi infection is usually made indirectly by the detection of specific antibodies to T. cruzi antigens. Following initial diagnostic evaluation or screening test (qualifying or discarding blood donation), a confirmation test is performed for samples initially reactive. The test presented in this study aims at the confirmation/refutation of the infectious status of human blood samples and will permit taking appropriate clinical measures. METHODOLOGY/PRINCIPAL FINDINGS:We designed a novel array of twelve antigens and printed these antigens onto 96-well plates. We tested 248 positive samples T. cruzi, 94 unscreened blood donors' samples from non-endemic area, 49 seronegative blood donors, 7 false-positive and 3 doubtful samples. The observed reactivities were analyzed to propose a decision-tree algorithm that correctly classifies all the samples, with the potential to discriminate false-positive results and sticky samples. We observed that antibodies levels (Sum of all antigens) was significantly higher for PCR positive than for PCR negative samples in all studied groups with Multi-cruzi. CONCLUSION/SIGNIFICANCE:The results described in this study indicate that the Multi-cruzi improves the serological confirmation of Chagas disease. Moreover the "sum of all antigens" detected by Multi-cruzi could reflect parasitemia level in patients-like PCR signals does-and could serve as an indicator of parasite clearance in longitudinal follow-ups. Validation of this assay is still required on an independent large collection of well characterized samples including typical false-reactive samples such as Leishmaniasis.
Keywords	Arctic medicine. Tropical medicine ; RC955-962 ; Public aspects of medicine ; RA1-1270
Subject code	630
Language	English
Publishing date	2016-04-01T00:00:00Z
Publisher	Public Library of Science (PLoS)
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Developing human capital in clinical research

Edward L. Murphy / Evan M. Bloch / Brian Custer / Caroline Shiboski / Ester Sabino / Anna Barbara Carneiro-Proietti / Willi McFarland / Michael P. Busch / Syria Laperche

Annals of Global Health, Vol 82, Iss

Impact on reducing transfusion-transmitted HIV and hepatitis virus infections in Africa

2016 Volume 3

Keywords	Infectious and parasitic diseases ; RC109-216 ; Public aspects of medicine ; RA1-1270
Language	English
Publishing date	2016-08-01T00:00:00Z
Publisher	Ubiquity Press
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: A comprehensive protocol to evaluate the use of blood and its components in Latin America and the Caribbean

Ana E. del Pozo / Maria D. Pérez-Rosales / Cesar de Almeida-Neto / Mirta C. Remesar / Armando D. Cortes / Raquel Baumgratz Delgado / Alfredo Mendrone Jr. / Ester Sabino

Revista Panamericana de Salud Pública, Vol 37, Iss 6, Pp 435-

2015 Volume 441

Abstract: Blood transfusion safety is a critical part of appropriate health care. Considering the limited information available on the use of blood and its components in Latin America and the Caribbean, the Grupo Cooperativo iberoamericano de Medicina ... ...

Abstract	Blood transfusion safety is a critical part of appropriate health care. Considering the limited information available on the use of blood and its components in Latin America and the Caribbean, the Grupo Cooperativo iberoamericano de Medicina Transfusional (Ibero-American Cooperative Group for Transfusion Medicine; GCIAMT), through its Research and International Affairs committees, carried out a project to develop a protocol that would facilitate the evaluation of blood usage at the country, jurisdiction, and institutional levels in varied country contexts. Experts in blood safety from the Pan American Health Organization (Washington, DC, United States), the University of São Paulo (São Paulo, Brazil), the Hemocentro of São Paulo (São Paulo, Brazil), and GCIAMT designed a 2-step comprehensive blood-use evaluation protocol: step 1 collects data from blood requests, and step 2, from medical charts. At a minimum, 1 000 analyzed requests are necessary; as such, study periods vary depending on the number of transfusion requests issued. An Internet-based application, the Modular Research System-Study Management System (MRS-SMS), houses the data and produces reports on how hospitals request blood, how blood is issued, who requires blood and blood components, and as an added benefit, how many blood units are wasted and what the real demand for blood is.
Keywords	Transfusión sanguínea ; protocolos ; bancos de sangre ; servicio de hemoterapia ; sistemas de administración de bases de datos ; seguridad de la sangre ; transfusión de componentes sanguíneos ; América Latina ; Región del Caribe ; Medicine ; R ; Arctic medicine. Tropical medicine ; RC955-962 ; Public aspects of medicine ; RA1-1270
Language	English
Publishing date	2015-06-01T00:00:00Z
Publisher	Pan American Health Organization
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Official communique

Dante Langhi Júnior / Dimas Tadeu Covas / Celso Bianco / Ester Sabino / José Francisco Comenalli Marques / José Eduardo Levi / Nanci Alves Salles / Neiva Sellan Lopes Gonçales / Simone Kashima

Revista Brasileira de Hematologia e Hemoterapia, Vol 36, Iss 5, Pp 309-

Chikungunya virus - a press release of the Associação Brasileira de Hematologia, Hemoterapia e Terapia Celular regarding the safety of transfusions and transplants

2014 Volume 310

Keywords	Internal medicine ; RC31-1245 ; Medicine ; R
Language	English
Publishing date	2014-10-01T00:00:00Z
Publisher	Sociedade Brasileira de Hematologia e Hemoterapia
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Chronic lung lesions in COVID-19 survivors

Paulo A Lotufo / Juliana C Ferreira / Eloisa Bonfa / Anna S Levin / Rodrigo Caruso Chate / Marta Imamura / Esper G Kallas / Roger Chammas / Thais Mauad / Izabel Marcilio / Nelson Gouveia / Ricardo Nitrini / José Eduardo Krieger / Marcio Valente Yamada Sawamura / Michelle Louvaes Garcia / Cristiano Gomes / Guilherme Fonseca / Jorge Hallak / Luis Yu /

Marcio Mancini / Maria Elizabeth Rossi / Thiago Avelino-Silva / Edivaldo M Utiyama / Aluisio C Segurado / Beatriz Perondi / Anna Miethke-Morais / Amanda C Montal / Leila Harima / Marjorie F Silva / Marcelo C Rocha / Maria Amélia de Jesus / Carolina Carmo / Clarice Tanaka / Julio F M Marchini / Thaís Guimarães / Ester Sabino / Carlos Roberto Ribeiro Carvalho / Celina Almeida Lamas / Diego Armando Cardona Cardenas / Daniel Mario Lima / Paula Gobi Scudeller / João Marcos Salge / Cesar Higa Nomura / Marco Antonio Gutierrez / Adriana L Araújo / Bruno F Guedes / Carolina S Lázari / Cassiano C Antonio / Claudia C Leite / Emmanuel A Burdmann

BMJ Open, Vol 12, Iss

predictive clinical model

2022 Volume 6

Keywords	Medicine ; R
Language	English
Publishing date	2022-06-01T00:00:00Z
Publisher	BMJ Publishing Group
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article: Gene expression profile in long-term non progressor HIV infected patients: In search of potential resistance factors

Luque, Maria Carolina / Aluisio C. Segurado / Camila C. Santos / Edecio Cunha-Neto / Eliane C. Mairena / Ester Sabino / GenÃ¨vieve Boucher / Jorge E. Kalil / Luiz A. Fonseca / Peter Wilkinson

Molecular Immunology. 2014 Nov., v. 62

2014

Abstract: Long-term non-progressors (LTNP) represent a minority (1â5%) of HIV-infected individuals characterized by documented infection for more than 7â10 years, a stable CD4+ T cell count over 500/mm3 and low viremia in the absence of antiretroviral ... ...

Abstract	Long-term non-progressors (LTNP) represent a minority (1â5%) of HIV-infected individuals characterized by documented infection for more than 7â10 years, a stable CD4+ T cell count over 500/mm3 and low viremia in the absence of antiretroviral treatment. Protective factors described so far such as the CCR5delta32 deletion, protective HLA alleles, or defective viruses fail to fully explain the partial protection phenotype. The existence of additional host resistance mechanisms in LTNP patients was investigated here using a whole human genome microarray study comparing gene expression profiles of unstimulated peripheral blood mononuclear cells from LTNP patients, HIV-1 infected patients under antiretroviral therapy with CD4+ T cell levels above 500/mm3 (ST), as well as healthy individuals. Genes that were up- or downregulated exclusively in LTNP, ST or in both groups in comparison to controls were identified and classified in functional categories using Ingenuity Pathway Analysis. ST and LTNP patient groups revealed distinct genetic profiles, regarding gene number in each category and up- or downregulation of specific genes, which could have a bearing on the outcome of each group. We selected some relevant genes to validate the differential expression using quantitative real-time qRT-PCR. Among others, we found several genes related to the canonical Wnt/beta-catenin signaling pathway. Our results identify new possible host genes and molecules that could be involved in the mechanisms leading to the slower progression to AIDS and sustained CD4+ T cell counts that is peculiar to LTNP patients.
Keywords	alleles ; antiretroviral agents ; CD4-positive T-lymphocytes ; gene expression ; gene expression regulation ; HIV infections ; Human immunodeficiency virus 1 ; humans ; microarray technology ; patients ; phenotype ; quantitative polymerase chain reaction ; resistance mechanisms ; reverse transcriptase polymerase chain reaction ; signal transduction ; therapeutics ; viremia ; viruses
Language	English
Dates of publication	2014-11
Size	p. 63-70.
Publishing place	Elsevier Ltd
Document type	Article
ZDB-ID	424427-8
ISSN	1872-9142 ; 0161-5890
ISSN (online)	1872-9142
ISSN	0161-5890
DOI	10.1016/j.molimm.2014.05.016
Database	NAL-Catalogue (AGRICOLA)

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Article ; Online: Genetic Characterization of a Panel of Diverse HIV-1 Isolates at Seven International Sites.

PLoS ONE, Vol 11, Iss 6, p e

2016 Volume 0157340

Abstract: HIV-1 subtypes and drug resistance are routinely tested by many international surveillance groups. However, results from different sites often vary. A systematic comparison of results from multiple sites is needed to determine whether a standardized ... ...

Abstract	HIV-1 subtypes and drug resistance are routinely tested by many international surveillance groups. However, results from different sites often vary. A systematic comparison of results from multiple sites is needed to determine whether a standardized protocol is required for consistent and accurate data analysis. A panel of well-characterized HIV-1 isolates (N = 50) from the External Quality Assurance Program Oversight Laboratory (EQAPOL) was assembled for evaluation at seven international sites. This virus panel included seven subtypes, six circulating recombinant forms (CRFs), nine unique recombinant forms (URFs) and three group O viruses. Seven viruses contained 10 major drug resistance mutations (DRMs). HIV-1 isolates were prepared at a concentration of 107 copies/ml and compiled into blinded panels. Subtypes and DRMs were determined with partial or full pol gene sequences by conventional Sanger sequencing and/or Next Generation Sequencing (NGS). Subtype and DRM results were reported and decoded for comparison with full-length genome sequences generated by EQAPOL. The partial pol gene was amplified by RT-PCR and sequenced for 89.4%-100% of group M viruses at six sites. Subtyping results of majority of the viruses (83%-97.9%) were correctly determined for the partial pol sequences. All 10 major DRMs in seven isolates were detected at these six sites. The complete pol gene sequence was also obtained by NGS at one site. However, this method missed six group M viruses and sequences contained host chromosome fragments. Three group O viruses were only characterized with additional group O-specific RT-PCR primers employed by one site. These results indicate that PCR protocols and subtyping tools should be standardized to efficiently amplify diverse viruses and more consistently assign virus genotypes, which is critical for accurate global subtype and drug resistance surveillance. Targeted NGS analysis of partial pol sequences can serve as an alternative approach, especially for detection of low-abundance DRMs.
Keywords	Medicine ; R ; Science ; Q
Subject code	572
Language	English
Publishing date	2016-01-01T00:00:00Z
Publisher	Public Library of Science (PLoS)
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Estimated prevalence of immunity to poliomyelitis in the city of São Paulo, Brazil

Carlos Roberto Veiga Kiffer / Orlando Jorge Conceição / Edgar Bortholi Santos / Ester Sabino / Roberto Focaccia

Brazilian Journal of Infectious Diseases, Vol 6, Iss 5, Pp 232-

a population-based survey

2002 Volume 243

Abstract: OBJECTIVES: Estimate the prevalence of immunity to poliomyelitis (anti-polio antibodies) in the city of São Paulo/Brazil through a population-based survey. METHODS: A quantitative and inductive method was used to draw a representative sample of the ... ...

Abstract	OBJECTIVES: Estimate the prevalence of immunity to poliomyelitis (anti-polio antibodies) in the city of São Paulo/Brazil through a population-based survey. METHODS: A quantitative and inductive method was used to draw a representative sample of the population. Randomization and stratification (based on sex, age and residence region) was done, and 1,059 individuals were studied on a home-visit basis (structured questionnaires and blood samples). A microneutralization test was performed to detect anti-polio antibodies against serotypes 1, 2 and 3. RESULTS: The estimated prevalence of immunity to poliomyelitis was high, with 94.6% prevalence of anti-polio 1 antibodies, 98.8% anti-polio 2 and 91.9% anti-polio 3. Despite this high prevalence, there were significantly lower prevalence levels in some groups, specially among age and residence region groups. DISCUSSION: Routine child immunization and NIDs with OPV have provided excellent levels of serological immunity to poliomyelitis in the population of the city of São Paulo, Brazil. However, there may be specific groups with a lower prevalence of immunity. Estimations of the prevalence of immunity to poliomyelitis were made in a population-based survey, which could be used as an auxiliary tool for supporting the polio eradication program.
Keywords	Immunity prevalence ; poliomyelitis ; poliviruses ; seroepidemiologic studies ; antibodies ; viral blood ; Infectious and parasitic diseases ; RC109-216 ; Internal medicine ; RC31-1245 ; Medicine ; R ; DOAJ:Internal medicine ; DOAJ:Medicine (General) ; DOAJ:Health Sciences
Subject code	380
Language	English
Publishing date	2002-10-01T00:00:00Z
Publisher	Brazilian Society of Infectious Diseases
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Use and misuse of biomarkers and the role of D-dimer and C-reactive protein in the management of COVID-19

Fabio Augusto Rodrigues Gonçalves / Bruno Adler Maccagnan Pinheiro Besen / Clarice Antunes de Lima / Aline Pivetta Corá / Antônio José Rodrigues Pereira / Sandro Félix Perazzio / Christiane Pereira Gouvea / Luiz Augusto Marcondes Fonseca / Evelinda Marramon Trindade / Nairo Massakazu Sumita / Alberto José da Silva Duarte / Arnaldo Lichtenstein / Eloisa Bonfa / Edivaldo M. Utiyama / Aluisio C. Segurado / Beatriz Perondi / Anna Miethke-Morais / Amanda C. Montal / Leila Harima /

Solange R. G. Fusco / Marjorie F. Silva / Marcelo C. Rocha / Izabel Marcilio / Izabel Cristina Rios / Fabiane Yumi Ogihara Kawano / Maria Amélia de Jesus / Ésper George Kallas / Carolina Carmo / Clarice Tanaka / Heraldo Possolo de Souza / Julio F. M. Marchini / Carlos Carvalho / Juliana C. Ferreira / Anna Sara Shafferman Levin / Maura Salaroli Oliveira / Thaís Guimarães / Carolina dos Santos Lázari / Ester Sabino / Marcello M. C. Magri / Tarcisio E. P. Barros-Filho / Maria Cristina Peres Braido Francisco / Silvia F. Costa

Clinics, Vol

A post-hoc analysis of a prospective cohort study

2021 Volume 76

Abstract: OBJECTIVE: Coronavirus disease 2019 (COVID-19) is associated with high mortality among hospitalized patients and incurs high costs. Severe acute respiratory syndrome coronavirus 2 infection can trigger both inflammatory and thrombotic processes, and ... ...

Abstract	OBJECTIVE: Coronavirus disease 2019 (COVID-19) is associated with high mortality among hospitalized patients and incurs high costs. Severe acute respiratory syndrome coronavirus 2 infection can trigger both inflammatory and thrombotic processes, and these complications can lead to a poorer prognosis. This study aimed to evaluate the association and temporal trends of D-dimer and C-reactive protein (CRP) levels with the incidence of venous thromboembolism (VTE), hospital mortality, and costs among inpatients with COVID-19. METHODS: Data were extracted from electronic patient records and laboratory databases. Crude and adjusted associations for age, sex, number of comorbidities, Sequential Organ Failure Assessment score at admission, and D-dimer or CRP logistic regression models were used to evaluate associations. RESULTS: Between March and June 2020, COVID-19 was documented in 3,254 inpatients. The D-dimer level ≥4,000 ng/mL fibrinogen equivalent unit (FEU) mortality odds ratio (OR) was 4.48 (adjusted OR: 1.97). The CRP level ≥220 mg/dL OR for death was 7.73 (adjusted OR: 3.93). The D-dimer level ≥4,000 ng/mL FEU VTE OR was 3.96 (adjusted OR: 3.26). The CRP level ≥220 mg/dL OR for VTE was 2.71 (adjusted OR: 1.92). All these analyses were statistically significant (p<0.001). Stratified hospital costs demonstrated a dose-response pattern. Adjusted D-dimer and CRP levels were associated with higher mortality and doubled hospital costs. In the first week, elevated D-dimer levels predicted VTE occurrence and systemic inflammatory harm, while CRP was a hospital mortality predictor. CONCLUSION: D-dimer and CRP levels were associated with higher hospital mortality and a higher incidence of VTE. D-dimer was more strongly associated with VTE, although its discriminative ability was poor, while CRP was a stronger predictor of hospital mortality. Their use outside the usual indications should not be modified and should be discouraged.
Keywords	COVID-19 ; Biomarkers ; Cohort Studies ; Venous Thromboembolism ; Health Care Costs ; Medicine (General) ; R5-920
Subject code	310
Language	English
Publishing date	2021-12-01T00:00:00Z
Publisher	Elsevier España
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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