LIVIVO - The Search Portal for Life Sciences

zur deutschen Oberfläche wechseln
Advanced search

Search results

Result 1 - 10 of total 13

Search options

  1. Article ; Online: COVID‐19

    Evangelos Andreakos / Sotirios Tsiodras

    EMBO Molecular Medicine, Vol 12, Iss 6, Pp n/a-n/a (2020)

    lambda interferon against viral load and hyperinflammation

    2020  

    Abstract: Coronavirus disease 2019 (COVID‐19), triggered by the betacoronavirus SARS‐CoV‐2, has become one of the worst pandemics of our time that has already caused more than 250,000 deaths (JHU data‐05/06/2020, https://coronavirus.jhu.edu/). Effective ... ...

    Abstract Coronavirus disease 2019 (COVID‐19), triggered by the betacoronavirus SARS‐CoV‐2, has become one of the worst pandemics of our time that has already caused more than 250,000 deaths (JHU data‐05/06/2020, https://coronavirus.jhu.edu/). Effective therapeutic approaches are urgently needed to reduce the spread of the virus and its death toll. Here, we assess the possibility of using interferon‐lambda (IFNλ), a third type of interferon sharing low homology with type I IFNs and IL‐10, for treating COVID‐19 patients. We discuss the unique role of IFNλ in fine‐tuning antiviral immunity in the respiratory tract to achieve optimal protection and minimal host damage and review early evidence that SARS‐CoV‐2 may impair IFNλ induction, leading to a delayed type I IFN‐dominated response that triggers hyperinflammation and severe disease. We also consider the potential windows of opportunity for therapeutic intervention with IFNλ and potential safety considerations. We conclude that IFNλ constitutes a promising therapeutic agent for reducing viral presence and hyperinflammation in a single shot to prevent the devastating consequences of COVID‐19 such as pneumonia and acute respiratory distress syndrome (ARDS).
    Keywords COVID‐19 ; interferon ; viral infection ; hyperinflammation ; cytokine storm ; Medicine (General) ; R5-920 ; Genetics ; QH426-470 ; covid19
    Subject code 610
    Language English
    Publishing date 2020-06-01T00:00:00Z
    Publisher Wiley
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

    Full text online

    More links

    Kategorien

    Inter-library loan at ZB MED

    Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

  2. Article ; Online: Protocol for influenza A virus infection of mice and viral load determination

    Ioanna-Evdokia Galani / Vasiliki Triantafyllia / Evridiki-Evangelia Eleminiadou / Evangelos Andreakos

    STAR Protocols, Vol 3, Iss 1, Pp 101151- (2022)

    2022  

    Abstract: Summary: Influenza A viruses (IAVs) are common respiratory viruses. Mouse models of IAV infection are valuable to study the mechanisms of IAV infection and pathology. Here, we present a detailed protocol for IAV infection of mice via intranasal ... ...

    Abstract Summary: Influenza A viruses (IAVs) are common respiratory viruses. Mouse models of IAV infection are valuable to study the mechanisms of IAV infection and pathology. Here, we present a detailed protocol for IAV infection of mice via intranasal administration. We detail the processing of mouse lung tissue and then describe the determination of viral load by several approaches including RNA, protein, or plaque-forming unit assays. This protocol may be adapted to other influenza strains or respiratory viruses.For complete details on the use and execution of this protocol, please refer to Galani et al. (2017).
    Keywords Flow Cytometry/Mass Cytometry ; Immunology ; Microbiology ; Microscopy ; Model Organisms ; Molecular Biology ; Science (General) ; Q1-390
    Language English
    Publishing date 2022-03-01T00:00:00Z
    Publisher Elsevier
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

    Full text online

    More links

    Kategorien

    Inter-library loan at ZB MED

    Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

  3. Article ; Online: Respiratory eukaryotic virome expansion and bacteriophage deficiency characterize childhood asthma

    Spyridon Megremis / Bede Constantinides / Paraskevi Xepapadaki / Chuan Fu Yap / Alexandros G. Sotiropoulos / Claus Bachert / Susetta Finotto / Tuomas Jartti / Avraam Tapinos / Tytti Vuorinen / Evangelos Andreakos / David L. Robertson / Nikolaos G. Papadopoulos

    Scientific Reports, Vol 13, Iss 1, Pp 1-

    2023  Volume 14

    Abstract: Abstract Asthma development and exacerbation is linked to respiratory virus infections. There is limited information regarding the presence of viruses during non-exacerbation/infection periods. We investigated the nasopharyngeal/nasal virome during a ... ...

    Abstract Abstract Asthma development and exacerbation is linked to respiratory virus infections. There is limited information regarding the presence of viruses during non-exacerbation/infection periods. We investigated the nasopharyngeal/nasal virome during a period of asymptomatic state, in a subset of 21 healthy and 35 asthmatic preschool children from the Predicta cohort. Using metagenomics, we described the virome ecology and the cross-species interactions within the microbiome. The virome was dominated by eukaryotic viruses, while prokaryotic viruses (bacteriophages) were independently observed with low abundance. Rhinovirus B species consistently dominated the virome in asthma. Anelloviridae were the most abundant and rich family in both health and asthma. However, their richness and alpha diversity were increased in asthma, along with the co-occurrence of different Anellovirus genera. Bacteriophages were richer and more diverse in healthy individuals. Unsupervised clustering identified three virome profiles that were correlated to asthma severity and control and were independent of treatment, suggesting a link between the respiratory virome and asthma. Finally, we observed different cross-species ecological associations in the healthy versus the asthmatic virus-bacterial interactome, and an expanded interactome of eukaryotic viruses in asthma. Upper respiratory virome “dysbiosis” appears to be a novel feature of pre-school asthma during asymptomatic/non-infectious states and merits further investigation.
    Keywords Medicine ; R ; Science ; Q
    Language English
    Publishing date 2023-05-01T00:00:00Z
    Publisher Nature Portfolio
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

    Full text online

    More links

    Kategorien

    Inter-library loan at ZB MED

    Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

  4. Article ; Online: COVID-19 enters the expanding network of apolipoprotein E4-related pathologies

    Kalliopi Gkouskou / Theodora Vasilogiannakopoulou / Evangelos Andreakos / Nikolaos Davanos / Maria Gazouli / Despina Sanoudou / Aristides G. Eliopoulos

    Redox Biology, Vol 41, Iss , Pp 101938- (2021)

    2021  

    Abstract: COVID-19 incidence and case fatality rates (CFR) differ among ethnicities, stimulating efforts to pinpoint genetic factors that could explain these phenomena. In this regard, the multiallelic apolipoprotein E (APOE) gene has recently been interrogated in ...

    Abstract COVID-19 incidence and case fatality rates (CFR) differ among ethnicities, stimulating efforts to pinpoint genetic factors that could explain these phenomena. In this regard, the multiallelic apolipoprotein E (APOE) gene has recently been interrogated in the UK biobank cohort, demonstrating associations of the APOE ε4/ε4 genotype with COVID-19 severity and mortality. The frequency of the ε4 allele and thus the distribution of APOE ε4/ε4 genotype may differ among populations. We have assessed APOE genotypes in 1638 Greek individuals, based on haplotypes derived from SNP rs7412 and rs429358 and found reduced frequency of ε4/ε4 compared to the British cohort. Herein we discuss this finding in relation to CFR and hypothesize on the potential mechanisms linking APOE ε4/ε4 to severe COVID-19. We postulate that the metabolic deregulation ensued by APOE4, manifested by elevated cholesterol and oxidized lipoprotein levels, may be central to heightened pneumocyte susceptibility to infection and to exaggerated lung inflammation associated with the ε4/ε4 genotype. We also discuss putative dietary and pharmacological approaches for the prevention and management of COVID-19 in APOE ε4/ε4 individuals.
    Keywords COVID-19 ; SARS-CoV-2 ; APOE4 ; ε4/ε4 ; Genetics ; Cholesterol ; Medicine (General) ; R5-920 ; Biology (General) ; QH301-705.5
    Language English
    Publishing date 2021-05-01T00:00:00Z
    Publisher Elsevier
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

    Full text online

    More links

    Kategorien

    Inter-library loan at ZB MED

    Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

  5. Article ; Online: C-type lectin receptor CLEC4A2 promotes tissue adaptation of macrophages and protects against atherosclerosis

    Inhye Park / Michael E. Goddard / Jennifer E. Cole / Natacha Zanin / Leo-Pekka Lyytikäinen / Terho Lehtimäki / Evangelos Andreakos / Marc Feldmann / Irina Udalova / Ignat Drozdov / Claudia Monaco

    Nature Communications, Vol 13, Iss 1, Pp 1-

    2022  Volume 17

    Abstract: The contribution of distinct subsets of macrophages to atherosclerosis is poorly understood. Here the authors describe a protective subset of vascular macrophages expressing the C-type lectin receptor CLEC4A2, which licenses monocytes to join the ... ...

    Abstract The contribution of distinct subsets of macrophages to atherosclerosis is poorly understood. Here the authors describe a protective subset of vascular macrophages expressing the C-type lectin receptor CLEC4A2, which licenses monocytes to join the resident vascular macrophage pool and ensures vascular homeostasis.
    Keywords Science ; Q
    Language English
    Publishing date 2022-01-01T00:00:00Z
    Publisher Nature Portfolio
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

    Full text online

    More links

    Kategorien

    Inter-library loan at ZB MED

    Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

  6. Article ; Online: A Multimodal Approach for the Risk Prediction of Intensive Care and Mortality in Patients with COVID-19

    Vasileios C. Pezoulas / Konstantina D. Kourou / Costas Papaloukas / Vassiliki Triantafyllia / Vicky Lampropoulou / Eleni Siouti / Maria Papadaki / Maria Salagianni / Evangelia Koukaki / Nikoletta Rovina / Antonia Koutsoukou / Evangelos Andreakos / Dimitrios I. Fotiadis

    Diagnostics, Vol 12, Iss 56, p

    2022  Volume 56

    Abstract: Background: Although several studies have been launched towards the prediction of risk factors for mortality and admission in the intensive care unit (ICU) in COVID-19, none of them focuses on the development of explainable AI models to define an ICU ... ...

    Abstract Background: Although several studies have been launched towards the prediction of risk factors for mortality and admission in the intensive care unit (ICU) in COVID-19, none of them focuses on the development of explainable AI models to define an ICU scoring index using dynamically associated biological markers. Methods: We propose a multimodal approach which combines explainable AI models with dynamic modeling methods to shed light into the clinical features of COVID-19. Dynamic Bayesian networks were used to seek associations among cytokines across four time intervals after hospitalization. Explainable gradient boosting trees were trained to predict the risk for ICU admission and mortality towards the development of an ICU scoring index. Results: Our results highlight LDH, IL-6, IL-8, Cr, number of monocytes, lymphocyte count, TNF as risk predictors for ICU admission and survival along with LDH, age, CRP, Cr, WBC, lymphocyte count for mortality in the ICU, with prediction accuracy 0.79 and 0.81, respectively. These risk factors were combined with dynamically associated biological markers to develop an ICU scoring index with accuracy 0.9. Conclusions: to our knowledge, this is the first multimodal and explainable AI model which quantifies the risk of intensive care with accuracy up to 0.9 across multiple timepoints.
    Keywords COVID-19 ; artificial intelligence ; dynamic modeling ; risk predictors ; ICU scoring index ; Medicine (General) ; R5-920
    Subject code 310
    Language English
    Publishing date 2022-12-01T00:00:00Z
    Publisher MDPI AG
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

    Full text online

    More links

    Kategorien

    Inter-library loan at ZB MED

    Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

  7. Article ; Online: Autoantibodies are highly prevalent in non–SARS-CoV-2 respiratory infections and critical illness

    Allan Feng / Emily Y. Yang / Andrew Reese Moore / Shaurya Dhingra / Sarah Esther Chang / Xihui Yin / Ruoxi Pi / Elisabeth K.M. Mack / Sara Völkel / Reinhard Geßner / Margrit Gündisch / Andreas Neubauer / Harald Renz / Sotirios Tsiodras / Paraskevi C. Fragkou / Adijat A. Asuni / Joseph E. Levitt / Jennifer G. Wilson / Michelle Leong /
    Jennifer H. Lumb / Rong Mao / Kassandra Pinedo / Jonasel Roque / Christopher M. Richards / Mikayla Stabile / Gayathri Swaminathan / Maria L. Salagianni / Vasiliki Triantafyllia / Wilhelm Bertrams / Catherine A. Blish / Jan E. Carette / Jennifer Frankovich / Eric Meffre / Kari Christine Nadeau / Upinder Singh / Taia T. Wang / Eline T. Luning Prak / Susanne Herold / Evangelos Andreakos / Bernd Schmeck / Chrysanthi Skevaki / Angela J. Rogers / Paul J. Utz

    JCI Insight, Vol 8, Iss

    2023  Volume 3

    Abstract: The widespread presence of autoantibodies in acute infection with SARS-CoV-2 is increasingly recognized, but the prevalence of autoantibodies in non–SARS-CoV-2 infections and critical illness has not yet been reported. We profiled IgG autoantibodies in ... ...

    Abstract The widespread presence of autoantibodies in acute infection with SARS-CoV-2 is increasingly recognized, but the prevalence of autoantibodies in non–SARS-CoV-2 infections and critical illness has not yet been reported. We profiled IgG autoantibodies in 267 patients from 5 independent cohorts with non–SARS-CoV-2 viral, bacterial, and noninfectious critical illness. Serum samples were screened using Luminex arrays that included 58 cytokines and 55 autoantigens, many of which are associated with connective tissue diseases (CTDs). Samples positive for anti-cytokine antibodies were tested for receptor blocking activity using cell-based functional assays. Anti-cytokine antibodies were identified in > 50% of patients across all 5 acutely ill cohorts. In critically ill patients, anti-cytokine antibodies were far more common in infected versus uninfected patients. In cell-based functional assays, 11 of 39 samples positive for select anti-cytokine antibodies displayed receptor blocking activity against surface receptors for Type I IFN, GM-CSF, and IL-6. Autoantibodies against CTD-associated autoantigens were also commonly observed, including newly detected antibodies that emerged in longitudinal samples. These findings demonstrate that anti-cytokine and autoantibodies are common across different viral and nonviral infections and range in severity of illness.
    Keywords Autoimmunity ; Infectious disease ; Medicine ; R
    Subject code 616
    Language English
    Publishing date 2023-02-01T00:00:00Z
    Publisher American Society for Clinical investigation
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

    Full text online

    More links

    Kategorien

    Inter-library loan at ZB MED

    Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

  8. Article ; Online: CD8+ T cells in beige adipogenesis and energy homeostasis

    Maria Moysidou / Sevasti Karaliota / Elisavet Kodela / Maria Salagianni / Yassemi Koutmani / Antonia Katsouda / Konstantia Kodella / Panagiotis Tsakanikas / Styliani Ourailidou / Evangelos Andreakos / Nikolaos Kostomitsopoulos / Dimitris Skokos / Antonios Chatzigeorgiou / Kyoung-Jin Chung / Stefan Bornstein / Mark W. Sleeman / Triantafyllos Chavakis / Katia P. Karalis

    JCI Insight, Vol 3, Iss

    2018  Volume 5

    Abstract: Although accumulation of lymphocytes in the white adipose tissue (WAT) in obesity is linked to insulin resistance, it remains unclear whether lymphocytes also participate in the regulation of energy homeostasis in the WAT. Here, we demonstrate enhanced ... ...

    Abstract Although accumulation of lymphocytes in the white adipose tissue (WAT) in obesity is linked to insulin resistance, it remains unclear whether lymphocytes also participate in the regulation of energy homeostasis in the WAT. Here, we demonstrate enhanced energy dissipation in Rag1–/– mice, increased catecholaminergic input to subcutaneous WAT, and significant beige adipogenesis. Adoptive transfer experiments demonstrated that CD8+ T cell deficiency accounts for the enhanced beige adipogenesis in Rag1–/– mice. Consistently, we identified that CD8–/– mice also presented with enhanced beige adipogenesis. The inhibitory effect of CD8+ T cells on beige adipogenesis was reversed by blockade of IFN-γ. All together, our findings identify an effect of CD8+ T cells in regulating energy dissipation in lean WAT, mediated by IFN-γ modulation of the abundance of resident immune cells and of local catecholaminergic activity. Our results provide a plausible explanation for the clinical signs of metabolic dysfunction in diseases characterized by altered CD8+ T cell abundance and suggest targeting of CD8+ T cells as a promising therapeutic approach for obesity and other diseases with altered energy homeostasis.
    Keywords Metabolism ; Medicine ; R
    Subject code 570
    Language English
    Publishing date 2018-03-01T00:00:00Z
    Publisher American Society for Clinical investigation
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

    Full text online

    More links

    Kategorien

    Inter-library loan at ZB MED

    Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

  9. Article ; Online: Iron restriction inside macrophages regulates pulmonary host defense against Rhizopus species

    Angeliki M. Andrianaki / Irene Kyrmizi / Kalliopi Thanopoulou / Clara Baldin / Elias Drakos / Sameh S. M. Soliman / Amol C. Shetty / Carrie McCracken / Tonia Akoumianaki / Kostas Stylianou / Petros Ioannou / Charalampos Pontikoglou / Helen A. Papadaki / Maria Tzardi / Valerie Belle / Emilien Etienne / Anne Beauvais / George Samonis / Dimitrios P. Kontoyiannis /
    Evangelos Andreakos / Vincent M. Bruno / Ashraf S. Ibrahim / Georgios Chamilos

    Nature Communications, Vol 9, Iss 1, Pp 1-

    2018  Volume 17

    Abstract: Mucormycosis is a life-threatening respiratory fungal infection that typically occurs in patients with abnormalities in iron metabolism. Here the authors show that iron restriction inside the phagosome of macrophages is an essential component of the host ...

    Abstract Mucormycosis is a life-threatening respiratory fungal infection that typically occurs in patients with abnormalities in iron metabolism. Here the authors show that iron restriction inside the phagosome of macrophages is an essential component of the host defense against Rhizopus, the main species causing mucormycosis.
    Keywords Science ; Q
    Language English
    Publishing date 2018-08-01T00:00:00Z
    Publisher Nature Publishing Group
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

    Full text online

    More links

    Kategorien

    Inter-library loan at ZB MED

    Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

  10. Article ; Online: Author Correction

    Angeliki M. Andrianaki / Irene Kyrmizi / Kalliopi Thanopoulou / Clara Baldin / Elias Drakos / Sameh S. M. Soliman / Amol C. Shetty / Carrie McCracken / Tonia Akoumianaki / Kostas Stylianou / Petros Ioannou / Charalampos Pontikoglou / Helen A. Papadaki / Maria Tzardi / Valerie Belle / Emilien Etienne / Anne Beauvais / George Samonis / Dimitrios P. Kontoyiannis /
    Evangelos Andreakos / Vincent M. Bruno / Ashraf S. Ibrahim / Georgios Chamilos

    Nature Communications, Vol 9, Iss 1, Pp 1-

    Iron restriction inside macrophages regulates pulmonary host defense against Rhizopus species

    2018  Volume 1

    Abstract: The original version of this Article contained an error in the spelling of the author Emilien Etienne, which was incorrectly given as Emilien Ettiene. These errors have now been corrected in both the PDF and HTML versions of the Article. ...

    Abstract The original version of this Article contained an error in the spelling of the author Emilien Etienne, which was incorrectly given as Emilien Ettiene. These errors have now been corrected in both the PDF and HTML versions of the Article.
    Keywords Science ; Q
    Language English
    Publishing date 2018-11-01T00:00:00Z
    Publisher Nature Publishing Group
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

    Full text online

    More links

    Kategorien

    Inter-library loan at ZB MED

    Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

To top