Article ; Online: Differences between quantification of genotype 3 hepatitis C virus RNA by Versions 1.0 and 2.0 of the COBAS AmpliPrep/COBAS TaqMan HCV Test.
Clinical chemistry and laboratory medicine
2017 Volume 55, Issue 7, Page(s) 956–961
Abstract: Background: Differences between the designs of hepatitis C virus (HCV) viral load assays can result in genotype-related variability in RNA quantification. We tested paired aliquots of plasma specimens from HCV-infected individuals using two versions (v1. ...
| Abstract | Background: Differences between the designs of hepatitis C virus (HCV) viral load assays can result in genotype-related variability in RNA quantification. We tested paired aliquots of plasma specimens from HCV-infected individuals using two versions (v1.0 and v2.0) of the Roche COBAS AmpliPrep/COBAS TaqMan HCV Test (CAP/CTM HCV) and noted variability between results for a subset of specimens; we then sought to determine whether discrepant results were more prevalent among specific HCV genotypes. Methods: Archived and prospectively-collected plasma samples from 114 unique patients were tested using CAP/CTM HCV v1.0 and v2.0. The HCV genotype result for each patient was determined by retrospectively reviewing laboratory records. Results: All (46/46) specimens with quantifiable viral loads from patients with genotype 1 or 2 infection had CAP/CTM HCV v1.0 and v2.0 results that were within 0.5 log10 IU/mL; in contrast, only 3/11 (27.3%) from patients with HCV genotype 3 (mean difference, 0.56 log10 IU/mL higher with v2.0) and 0/3 (0%) from patients with HCV genotype 4 (mean difference, 0.91 log10 IU/mL higher with v2.0) had results within 0.5 log10 IU/mL. Among specimens with detectable HCV RNA below the lower limit of quantification with v1.0, greater proportions of genotype 3 (4/7, 57.1%) and genotype 4 (3/4, 75.0%) specimens than genotype 1 or 2 specimens (6/30, 20.0%) had v2.0 results within the quantifiable range. Conclusions: In patients infected with HCV genotype 3, sequential CAP/CTM HCV viral load results should be compared with caution and interpreted in the context of the specific assay version used. |
|---|---|
| MeSH term(s) | Genotype ; Hepacivirus/genetics ; Hepacivirus/physiology ; Humans ; Limit of Detection ; Nucleic Acid Amplification Techniques/methods ; RNA, Viral/analysis ; RNA, Viral/blood ; RNA, Viral/genetics ; Retrospective Studies ; Taq Polymerase/metabolism ; Viral Load |
| Chemical Substances | RNA, Viral ; Taq Polymerase (EC 2.7.7.-) |
| Language | English |
| Publishing date | 2017-06-27 |
| Publishing country | Germany |
| Document type | Comparative Study ; Journal Article |
| ZDB-ID | 1418007-8 |
| ISSN | 1437-4331 ; 1434-6621 ; 1437-8523 |
| ISSN (online) | 1437-4331 |
| ISSN | 1434-6621 ; 1437-8523 |
| DOI | 10.1515/cclm-2016-0799 |
| Database | MEDical Literature Analysis and Retrieval System OnLINE |
More links
Kategorien
In stock of ZB MED Cologne/Königswinter
| Zs.A 121: Show issues | Location: Je nach Verfügbarkeit (siehe Angabe bei Bestand) bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular Jg. 1995 - 2021: Lesesall (1.OG) ab Jg. 2022: Lesesaal (EG) |
Order via subito
This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.