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  1. Article ; Online: Effect of IGFBP6 Knockdown on Proteins Regulating Exosome Synthesis and Secretion in MDA-MB-231 Cell Line.

    Efimova, A S / Antipenko, I D / Evtushenko, E A / Balan, P V / Tonevitskaya, S A

    Bulletin of experimental biology and medicine

    2023  Volume 175, Issue 1, Page(s) 157–161

    Abstract: One of the potential causes of cancer recurrence is disruption of the cell-cell communication in the primary tumors that is realized, among other things, through secretion and uptake of exosomes by cells. Low expression of the IGFBP6 gene (insulin-like ... ...

    Abstract One of the potential causes of cancer recurrence is disruption of the cell-cell communication in the primary tumors that is realized, among other things, through secretion and uptake of exosomes by cells. Low expression of the IGFBP6 gene (insulin-like growth factor binding protein 6) is associated with a high recurrence rate and can serve as a prognostic marker of luminal breast cancer. The knockdown of the IGFBP6 gene leads to significant changes in lipid metabolism. We performed a quantitative analysis of both exosomes and proteins involved in the mechanism of their biogenesis. Changes in the expression profile of mRNAs and their proteins responsible for the synthesis and secretion of exosomes were revealed. We showed a decrease in the expression of the of the VPS28 gene mRNA (vacuolar protein sorting-associated protein 28) and the corresponding protein by 2.3 and 5.6 times, respectively. The secretion of exosomes by MDA-MB-231 cells with IGFBP6 knockdown decreased by 2 times. We discussed a mechanism of disruption of cell-cell communication.
    MeSH term(s) Humans ; Insulin-Like Growth Factor Binding Protein 6/genetics ; Insulin-Like Growth Factor Binding Protein 6/metabolism ; MDA-MB-231 Cells ; Cell Line, Tumor ; Exosomes/genetics ; Exosomes/metabolism ; Gene Expression Regulation, Neoplastic ; Neoplasm Recurrence, Local/genetics
    Chemical Substances Insulin-Like Growth Factor Binding Protein 6
    Language English
    Publishing date 2023-06-19
    Publishing country United States
    Document type Journal Article
    ZDB-ID 390407-6
    ISSN 1573-8221 ; 0007-4888 ; 0365-9615
    ISSN (online) 1573-8221
    ISSN 0007-4888 ; 0365-9615
    DOI 10.1007/s10517-023-05828-9
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 56: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (1.OG)
    ab Jg. 2022: Lesesaal (EG)

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  2. Article ; Online: Dataset on safety and protective efficacy studies of COVID-19 vaccine candidates based on structurally modified plant virus in female hamsters.

    Kovalenko, A O / Ryabchevskaya, E M / Evtushenko, E A / Kondakova, O A / Ivanov, P A / Arkhipenko, M V / Nikitin, N A / Karpova, O V

    Data in brief

    2023  Volume 48, Page(s) 109158

    Abstract: This data article is related to the previous research, which addressed the development of a COVID-19 recombinant vaccine candidate. Here, we present the additional data in support of the safety and protective efficacy evaluation of two COVID-19 vaccine ... ...

    Abstract This data article is related to the previous research, which addressed the development of a COVID-19 recombinant vaccine candidate. Here, we present the additional data in support of the safety and protective efficacy evaluation of two COVID-19 vaccine candidates based on the coronaviruses' S protein fragments and a structurally modified plant virus - spherical particles. The effectiveness of the experimental vaccines was studied against the SARS-CoV-2 virus in an
    Language English
    Publishing date 2023-04-19
    Publishing country Netherlands
    Document type Journal Article
    ZDB-ID 2786545-9
    ISSN 2352-3409 ; 2352-3409
    ISSN (online) 2352-3409
    ISSN 2352-3409
    DOI 10.1016/j.dib.2023.109158
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article: Surface Charge Mapping on Virions and Virus-Like Particles of Helical Plant Viruses.

    Arkhipenko, M V / Nikitin, N A / Baranov, O A / Evtushenko, E A / Atabekov, J G / Karpova, O V

    Acta naturae

    2020  Volume 11, Issue 4, Page(s) 73–78

    Abstract: Currently, the assembly of helical plant viruses is poorly understood. The viral assembly and infection may be affected by the charge distribution on the virion surface. However, only the total virion charge (isoelectric point) has been determined for ... ...

    Abstract Currently, the assembly of helical plant viruses is poorly understood. The viral assembly and infection may be affected by the charge distribution on the virion surface. However, only the total virion charge (isoelectric point) has been determined for most plant viruses. Here, we report on the first application of positively charged magnetic nanoparticles for mapping the surface charge distribution of helical plant viruses. The charge was demonstrated to be unevenly distributed on the surface of viruses belonging to different taxonomic groups, with the negative charge being predominantly located at one end of the virions. This charge distribution is mainly controlled by viral RNA.
    Language English
    Publishing date 2020-01-18
    Publishing country Russia (Federation)
    Document type Journal Article
    ISSN 2075-8251
    ISSN 2075-8251
    DOI 10.32607/20758251-2019-11-4-73-78
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article: [A Recombinant Rotavirus Antigen Based on the Coat Protein of Alternanthera Mosaic Virus].

    Ryabchevskaya, E M / Evtushenko, E A / Arkhipenko, M V / Donchenko, E K / Nikitin, N A / Atabeko, J G / Karpova, O V

    Molekuliarnaia biologiia

    2020  Volume 54, Issue 2, Page(s) 278–284

    Abstract: Thanks to their strong immunostimulating properties and safety for humans, plant viruses represent an appropriate basis for the design of novel vaccines. The coat protein of Alternanthera mosaic virus can form virus-like particles that are stable under ... ...

    Abstract Thanks to their strong immunostimulating properties and safety for humans, plant viruses represent an appropriate basis for the design of novel vaccines. The coat protein of Alternanthera mosaic virus can form virus-like particles that are stable under physiological conditions and have adjuvant properties. This work presents a recombinant human rotavirus A antigen based on the epitope of rotavirus structural protein VP6, using Alternanthera mosaic virus coat protein as a carrier. An expression vector containing the gene of Alternanthera mosaic virus (MU strain) coat protein fused to the epitope of rotavirus protein VP6 was designed. Immunoblot analysis showed that the chimeric protein was effectively recognized by commercial polyclonal antibodies to rotavirus and therefore is a suitable candidate for development of a vaccine prototype. Interaction of the chimeric recombinant protein with the native coat protein of Alternanthera mosaic virus and its RNA resulted in the formation of ribonucleoprotein complexes that were recognized by anti-rotavirus antibodies.
    MeSH term(s) Antibodies, Viral ; Antigens, Viral/immunology ; Capsid Proteins/immunology ; Humans ; Potexvirus/immunology ; Recombinant Proteins/immunology ; Rotavirus/immunology
    Chemical Substances Alternanthera mosaic virus ; Antibodies, Viral ; Antigens, Viral ; Capsid Proteins ; Recombinant Proteins
    Language Russian
    Publishing date 2020-05-11
    Publishing country Russia (Federation)
    Document type Journal Article
    ZDB-ID 213542-5
    ISSN 0026-8984
    ISSN 0026-8984
    DOI 10.31857/S0026898420020135
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.B 1796: Show issues Location:
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  5. Article: A Recombinant Rotavirus Antigen Based on the Coat Protein of Alternanthera Mosaic Virus.

    Ryabchevskaya, E M / Evtushenko, E A / Arkhipenko, M V / Donchenko, E K / Nikitin, N A / Atabekov, J G / Karpova, O V

    Molecular biology

    2020  Volume 54, Issue 2, Page(s) 243–248

    Abstract: Thanks to their strong immunostimulating properties and safety for humans, plant viruses represent an appropriate basis for the design of novel vaccines. The coat protein of Alternanthera mosaic virus can form virus-like particles that are stable under ... ...

    Abstract Thanks to their strong immunostimulating properties and safety for humans, plant viruses represent an appropriate basis for the design of novel vaccines. The coat protein of Alternanthera mosaic virus can form virus-like particles that are stable under physiological conditions and have adjuvant properties. This work presents a recombinant human rotavirus A antigen based on the epitope of rotavirus structural protein VP6, using Alternanthera mosaic virus coat protein as a carrier. An expression vector containing the gene of Alternanthera mosaic virus (MU strain) coat protein fused to the epitope of rotavirus protein VP6 was designed. Immunoblot analysis showed that the chimeric protein was effectively recognized by commercial polyclonal antibodies to rotavirus and therefore is a suitable candidate for development of a vaccine prototype. Interaction of the chimeric recombinant protein with the native coat protein of Alternanthera mosaic virus and its RNA resulted in the formation of ribonucleoprotein complexes that were recognized by anti-rotavirus antibodies.
    Keywords covid19
    Language English
    Publishing date 2020-04-30
    Publishing country Russia (Federation)
    Document type Journal Article
    ZDB-ID 213541-3
    ISSN 1608-3245 ; 0026-8933
    ISSN (online) 1608-3245
    ISSN 0026-8933
    DOI 10.1134/S0026893320020132
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 1303: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (1.OG)
    ab Jg. 2022: Lesesaal (EG)

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