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  1. Article ; Online: Pleistocene Aquatic Refuges Support the East–West Separation of the Neotropical Catfish Trichomycterinae (Siluriformes: Trichomycteridae) and High Diversity in the Magdalena, Guiana, and Paraná-Paraguay Basins

    Volpi, Thais de Assis / Monjardim, Marina / Sarmento-Soares, Luisa M. / Fagundes, Valéria

    Diversity. 2023 Aug. 15, v. 15, no. 8

    2023  

    Abstract: 1) Background: Trichomycterinae represent 60% of the species in the family and, while seven genera comprise 1–3 species each, Trichomycterus and Cambeva have over 180 known species between them. Although integrative studies aimed to clarify the ... ...

    Abstract (1) Background: Trichomycterinae represent 60% of the species in the family and, while seven genera comprise 1–3 species each, Trichomycterus and Cambeva have over 180 known species between them. Although integrative studies aimed to clarify the relationships within the subfamily, the diversity of species of Trichomycterus remains an open question. Herein, we explored an unprecedented sample to investigate the divergence in the lineages of Trichomycterus. (2) Methods: we recovered the phylogenetic relationships of the subfamily using 566 sequences (999 bp) of the mitochondrial gene cytochrome b, calculated intra- and intergroup distance percentages, and estimated divergence times. (3) Results: we recovered 13 highly supported and geographically structured lineages; intergenus divergence was 11–20%, while interspecies divergence was 3–11%; Trichomycterus, Cambeva, Scleronema, Hatcheria, Eremophilus, and Ituglanis were recovered as monophyletic, with three other highly divergent clades: Guiana Shield, Magdalena basin, and Tapajós basin. (4) Conclusions: We propose that the trans-Andean austral clades be allocated into Hatcheria, and the Guiana clade supports a new genus. We also observed that the headwaters nearest the Magdalena and Orinoco basins showed a high diversity and endemism of Trichomycterinae lineages. We discussed the role of geomorphological events and the climatic features which may explain cladogenesis events in Trichomycterinae.
    Keywords Hatcheria ; Ituglanis ; Neotropics ; Pleistocene epoch ; Scleronema ; Trichomycterus ; basins ; biological speciation ; catfish ; cytochrome b ; indigenous species ; mitochondrial genes ; monophyly ; new genus ; species diversity
    Language English
    Dates of publication 2023-0815
    Publishing place Multidisciplinary Digital Publishing Institute
    Document type Article ; Online
    ZDB-ID 2518137-3
    ISSN 1424-2818
    ISSN 1424-2818
    DOI 10.3390/d15080929
    Database NAL-Catalogue (AGRICOLA)

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  2. Article: Penile shape discriminates two cryptic species of

    Campana, Leonardo / Cruz, Letícia Rosário / Paresque, Roberta / Fagundes, Valéria

    ZooKeys

    2022  Volume 1134, Page(s) 1–22

    Abstract: Glans penis morphology has been used as a powerful tool in mammal taxonomy to differentiate cryptic species. Neotropical rodent ... ...

    Abstract Glans penis morphology has been used as a powerful tool in mammal taxonomy to differentiate cryptic species. Neotropical rodent species
    Language English
    Publishing date 2022-12-05
    Publishing country Bulgaria
    Document type Journal Article
    ZDB-ID 2445640-8
    ISSN 1313-2970 ; 1313-2989
    ISSN (online) 1313-2970
    ISSN 1313-2989
    DOI 10.3897/zookeys.1134.89587
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article ; Online: Penile shape discriminates two cryptic species of Akodon Meyen, 1833 (Mammalia, Rodentia, Cricetidae) from eastern Brazil

    Campana, Leonardo / Cruz, Letícia Rosário / Paresque, Roberta / Fagundes, Valéria

    ZooKeys. 2022 Dec. 05, v. 1134 p.1-22

    2022  

    Abstract: AbstractGlans penis morphology has been used as a powerful tool in mammal taxonomy to differentiate cryptic species. Neotropical rodent species Akodon cursor and A. montensis are cryptic, and interspecific hybrids are like their parental species. We ... ...

    Abstract AbstractGlans penis morphology has been used as a powerful tool in mammal taxonomy to differentiate cryptic species. Neotropical rodent species Akodon cursor and A. montensis are cryptic, and interspecific hybrids are like their parental species. We investigated non-metric and metric phallic characters aiming to differentiate A. cursor from A. montensis. We also evaluated the parental species’ influence of the phallic characters on hybrids. We analysed 96 male adults—56 A. cursor, 27 A. montensis, and 13 hybrids, subgrouping species by locality and hybrids by parental species (paternal vs maternal). We verified that A. cursor and A. montensis are distinguishable by penile-shape morphology: A. cursor has an elongated penile form with a flare in the distal portion and A. montensis has a barrel-shaped form. Also, dark spots in ventral view, if present in A. montensis, distinguish A. montensis from A. cursor. Although the non-metric characters differentiate the species, they do not distinguish the subgroups of A. cursor, A. montensis, and hybrids. The metric phallic characters indicated a significant difference between species and hybrids. These characters also differentiate the population groups of A. cursor. However, A. montensis subgroups and hybrids subgroups did not present a significant difference. This study shows the importance of penis morphology in the taxonomy of the cryptic rodent species A. cursor and A. montensis, representing a powerful tool to discriminate male specimens in mammal collections without karyotyping or sequencing, even though the specimens occurred in sympatric areas. Since most taxidermy protocols do not preserve the penis in mammal preparations, liquid preservation of some specimens or the removal of the penis before taxidermy for liquid preservation could be beneficial. We also recommend the organisation in museum collections of a penis bank for the A. cursor species group (or even all rodent species) to avoid losing this important information for species identification.
    Keywords Cricetidae ; Neotropics ; cryptic species ; karyotyping ; liquids ; males ; museums ; penis ; rodents ; species identification ; sympatry ; Brazil ; Glans penis ; hybrids ; interspecific variation ; population variation
    Language English
    Dates of publication 2022-1205
    Size p. 1-22.
    Publishing place Pensoft Publishers
    Document type Article ; Online
    ZDB-ID 2445640-8
    ISSN 1313-2970 ; 1313-2989
    ISSN (online) 1313-2970
    ISSN 1313-2989
    DOI 10.3897/zookeys.1134.89587
    Database NAL-Catalogue (AGRICOLA)

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  4. Article: DNA barcoding and hypopygium shape support delimitation of sympatric

    Monjardim, Marina / Azevedo, Celso O / Fagundes, Valéria

    ZooKeys

    2020  Volume 959, Page(s) 87–97

    Abstract: ... ...

    Abstract Dissomphalus
    Language English
    Publishing date 2020-08-14
    Publishing country Bulgaria
    Document type Journal Article
    ZDB-ID 2445640-8
    ISSN 1313-2970 ; 1313-2989
    ISSN (online) 1313-2970
    ISSN 1313-2989
    DOI 10.3897/zookeys.959.53737
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article: DNA barcoding and hypopygium shape support delimitation of sympatric Dissomphalus species (Hymenoptera, Bethylidae) from the Atlantic rainforest

    Monjardim, Marina / Azevedo, Celso O / Fagundes, Valéria

    ZooKeys. 2020 Aug. 14, v. 959

    2020  

    Abstract: Dissomphalus is a cosmopolitan genus of Bethylidae and has 269 Neotropical species divided into 32 species-groups, mostly defined by the genital and the tergal process structures. Dissomphalus rectilineus and D. concavatus are sympatric species in the ... ...

    Abstract Dissomphalus is a cosmopolitan genus of Bethylidae and has 269 Neotropical species divided into 32 species-groups, mostly defined by the genital and the tergal process structures. Dissomphalus rectilineus and D. concavatus are sympatric species in the ulceratus species-group. Members of the species-group share many similarities in the morphology of the head, hypopygium, tergal process and genitalia, but may be distinguished by the structure of the hypopygium. Previous studies have found intermediate structures of the hypopygium in the sympatric areas and raised questions about the distinctiveness of these two species. We sequenced 340 bp of the mitochondrial gene cytochrome oxidase I of 29 specimens from Brazil and Paraguay, calculated the genetic divergence among specimens, and recovered the phylogenetic relationships between taxa. In addition, we compared the morphology of the hypopygium to evaluate its use as a species-specific diagnostic character using the genetic divergence values. We recovered three well-supported monophyletic groups (intraclade divergence from 1.3 to 13.4%) and three hypopygium morphologies associated with each clade, two of them associated with D. rectilineus and D. concavatus (as described in the literature); the third one is new, not associated with any known species. The divergence between the D. rectilineus and D. concavatus clades was 19%, while the third clade is divergent from each species by 19–20%. If fully described, the hypopygium shape associated with the COI sequence will represent an extremely promising approach to the diagnosis of Dissomphalus species.
    Keywords Bethylidae ; DNA barcoding ; Neotropics ; cytochrome-c oxidase ; genetic variation ; genitalia ; head ; mitochondrial genes ; monophyly ; rain forests ; sympatry ; Brazil ; Paraguay
    Language English
    Dates of publication 2020-0814
    Size p. 87-97.
    Publishing place Pensoft Publishers
    Document type Article
    Note NAL-light
    ZDB-ID 2445640-8
    ISSN 1313-2970 ; 1313-2989
    ISSN (online) 1313-2970
    ISSN 1313-2989
    DOI 10.3897/zookeys.959.53737
    Database NAL-Catalogue (AGRICOLA)

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  6. Article ; Online: Dysphonia and Dysarthria in People With Parkinson's Disease After Subthalamic Nucleus Deep Brain Stimulation: Effect of Frequency Modulation.

    Morello, Aline Nunes Da Cruz / Beber, Bárbara Costa / Fagundes, Valéria Carvalho / Cielo, Carla Aparecida / Rieder, Carlos R M

    Journal of voice : official journal of the Voice Foundation

    2018  Volume 34, Issue 3, Page(s) 477–484

    Abstract: Purpose: Subthalamic nucleus deep brain stimulation (STN-DBS) parameters, for example the frequency of stimulation, seem to affect speech and voice aspects. However, this influence is not well understood. This study aimed to investigate the impact of ... ...

    Abstract Purpose: Subthalamic nucleus deep brain stimulation (STN-DBS) parameters, for example the frequency of stimulation, seem to affect speech and voice aspects. However, this influence is not well understood. This study aimed to investigate the impact of low- and high-frequency STN-DBS on voice and speech for people with Parkinson's disease.
    Methods: Nineteen individuals with Parkinson's disease who received bilateral STN-DBS were assessed for motor performance (Unified Parkinson's Disease Rating Scale-III), perceptual evaluation of voice (grade, roughness, breathiness, asthenia, strain, and instability [GRBASI]), dysarthria assessment, and computerized acoustic analysis of voice upon receiving low-frequency (60 Hz) and high-frequency (130 Hz) STN-DBS.
    Results: In the GRBASI protocol, asthenia, and instability were significantly better at 130 Hz of stimulation. In the dysarthria evaluation, the phonation aspect, articulation, and grade of dysarthria showed deterioration at the same high-frequency condition. There was no significant difference for any vocal acoustic measures.
    Conclusion: The high-frequency of STN-DBS may affect speech and voice differently, leading to an amelioration of the vocal production, but with adverse effects in the speech control.
    MeSH term(s) Adult ; Aged ; Deep Brain Stimulation/adverse effects ; Dysarthria/diagnosis ; Dysarthria/etiology ; Dysarthria/physiopathology ; Dysphonia/diagnosis ; Dysphonia/etiology ; Dysphonia/physiopathology ; Female ; Humans ; Male ; Middle Aged ; Motor Activity ; Parkinson Disease/complications ; Parkinson Disease/diagnosis ; Parkinson Disease/physiopathology ; Parkinson Disease/therapy ; Phonation ; Recovery of Function ; Risk Factors ; Subthalamic Nucleus ; Treatment Outcome ; Voice Quality
    Language English
    Publishing date 2018-11-16
    Publishing country United States
    Document type Journal Article
    ZDB-ID 17459-2
    ISSN 1873-4588 ; 1557-8658 ; 0892-1997
    ISSN (online) 1873-4588 ; 1557-8658
    ISSN 0892-1997
    DOI 10.1016/j.jvoice.2018.10.012
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  7. Article ; Online: The use of Proteinase K to access genitalia morphology, vouchering and DNA extraction in minute wasps.

    Martinelli, Arturo B / Waichert, Cecilia / Barbosa, Diego N / Fagundes, Valéria / Azevedo, Celso O

    Anais da Academia Brasileira de Ciencias

    2017  Volume 89, Issue 3, Page(s) 1629–1633

    Abstract: Genitalia are rich source of characters in insect taxonomy. Usually, they are examined after dissection and cleaning with potassium hydroxide (KOH), procedure that may damage both genital morphological structures and intracellular molecular contents. ... ...

    Abstract Genitalia are rich source of characters in insect taxonomy. Usually, they are examined after dissection and cleaning with potassium hydroxide (KOH), procedure that may damage both genital morphological structures and intracellular molecular contents. Enzymatic procedure with Proteinase K has been used to clean muscle off the genitalia while extract DNA, but its damage to the genital structures has not been evaluated. Herein, we qualitatively compare the use of KOH and Proteinase K to prepare genital structures in minute insects (Hymenoptera: Bethylidae). We show that Proteinase K is better to preserve the genital structure and provides quality DNA.
    Language English
    Publishing date 2017-07
    Publishing country Brazil
    Document type Journal Article
    ZDB-ID 2046885-4
    ISSN 1678-2690 ; 0001-3765
    ISSN (online) 1678-2690
    ISSN 0001-3765
    DOI 10.1590/0001-3765201720160825
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  8. Article ; Online: Testing the Rio Doce as a riverine barrier in shaping the Atlantic rainforest population divergence in the rodent Akodon cursor.

    Colombi, Victor Hugo / Lopes, Silvia Ramira / Fagundes, Valéria

    Genetics and molecular biology

    2010  Volume 33, Issue 4, Page(s) 785–789

    Abstract: Akodon cursor occurs in dense rainforest from northern (8° S) to southern (26° S) states along the Atlantic coast of Brazil. Previous karyological and molecular data revealed two major clades, one including northern (8-15° S) and the other southern (19- ... ...

    Abstract Akodon cursor occurs in dense rainforest from northern (8° S) to southern (26° S) states along the Atlantic coast of Brazil. Previous karyological and molecular data revealed two major clades, one including northern (8-15° S) and the other southern (19-26° S) populations. The center of geographic distribution (15-20° S), which included the state of Espírito Santo, was identified as a potential vicariance region. Since river barriers are among the most discussed models in the study of Neotropical diversification, we examined whether the Rio Doce (19° S) plays an important role in shaping the population genetic divergence of A. cursor by including samples from Espírito Santo in the analysis. Our results showed that the northern-southern division region in Atlantic forest was no coincidence with the presence of the Rio Doce by refuting the hypothesis that this river is an effective barrier to gene flow between populations. Instead, we found evidence that isolation by geographical distance shaped the phylogeographical structure in the southern lineage. However, there is uncertainty about effectiveness of the processes involved and further studies based on wider sampling are needed.
    Language English
    Publishing date 2010-12-01
    Publishing country Brazil
    Document type Journal Article
    ZDB-ID 1445712-x
    ISSN 1678-4685 ; 1415-4757
    ISSN (online) 1678-4685
    ISSN 1415-4757
    DOI 10.1590/s1415-47572010000400029
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  9. Article: Karyology of the Atlantic forest rodent Juliomys (Cricetidae): A new karyotype from southern Brazil.

    Paresque, Roberta / Christoff, Alexandre Uarth / Fagundes, Valéria

    Genetics and molecular biology

    2009  Volume 32, Issue 2, Page(s) 301–305

    Abstract: Juliomys is a small rodent from the family Cricetidae which inhabits the Atlantic forest and forests from Argentina to eastern Brazil. The three species recognized so far have different karyotypes. In this paper, we describe a new karyotype with 2n = 32, ...

    Abstract Juliomys is a small rodent from the family Cricetidae which inhabits the Atlantic forest and forests from Argentina to eastern Brazil. The three species recognized so far have different karyotypes. In this paper, we describe a new karyotype with 2n = 32, FN = 48 found in Juliomys specimens from a high-altitude area in the Atlantic forest of southern Brazil. The karyotype was analyzed after G- and C-banding and silver staining of the nucleolus organizer regions (Ag-NOR) and its G-banding patterns were compared with those of the newly described species Juliomys ossitenuis (2n = 20, FN = 36). The 2n = 32 karyomorph presented peculiar features and was very different from those of the other species of the genus: J. pictipes (2n = 36, FN = 34), J. rimofrons (2n = 20, FN = 34) and J. ossitenuis (2n = 20, FN = 36). Differences were mostly due to centric and tandem fusions, pericentric inversion and loss of heterochromatin. The karyotype represents a powerful tool to differentiate Juliomys species and our data suggest that the karyotype described herein belongs to a new species.
    Language English
    Publishing date 2009-03-13
    Publishing country Brazil
    Document type Journal Article
    ZDB-ID 1445712-x
    ISSN 1678-4685 ; 1415-4757
    ISSN (online) 1678-4685
    ISSN 1415-4757
    DOI 10.1590/S1415-47572009005000031
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  10. Article: Deep Brain Stimulation Frequency of the Subthalamic Nucleus Affects Phonemic and Action Fluency in Parkinson's Disease.

    Fagundes, Valéria de Carvalho / Rieder, Carlos R M / da Cruz, Aline Nunes / Beber, Bárbara Costa / Portuguez, Mirna Wetters

    Parkinson's disease

    2016  Volume 2016, Page(s) 6760243

    Abstract: Introduction. ...

    Abstract Introduction.
    Language English
    Publishing date 2016-12-05
    Publishing country United States
    Document type Journal Article
    ZDB-ID 2573854-9
    ISSN 2042-0080 ; 2090-8083
    ISSN (online) 2042-0080
    ISSN 2090-8083
    DOI 10.1155/2016/6760243
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