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  1. Article ; Online: New strategies for sterilization and preservation of fresh fish skin grafts.

    Ibrahim, Ahmed / Fahmy, Hossam M / Mahmoud, Ghada Abd-Elmonsef / Soliman, Mahmoud / Elshahawy, Abdelnaby M

    Scientific reports

    2024  Volume 14, Issue 1, Page(s) 1253

    Abstract: The introduction of fish skin as a biological dressing for treating burns and wounds holds great promise, offering an alternative to existing management strategies. However, the risk of disease transmission is a significant concern. Therefore, this study ...

    Abstract The introduction of fish skin as a biological dressing for treating burns and wounds holds great promise, offering an alternative to existing management strategies. However, the risk of disease transmission is a significant concern. Therefore, this study aimed to examine how established sterilization and preservation procedures affected fish skin grafts' microbiological and histological properties for long-term usage. Lyophilization of the fish skin graft followed by rehydration in normal saline for 15 min did not change the collagen content. Furthermore, gamma irradiation of the lyophilized fish skin graft at different lengths 5, 10, and 25 KGy showed a significant reduction in microbial growth (aerobic bacteria, aerobic yeasts, and fungi) at 15- and 30 days after the irradiation. However, exposure to 10 KGy was found to be the most effective intensity among the different gamma irradiation lengths since it preserved the collagen fiber content and intensity in the lyophilized fish skin grafts at 15- and 30 days after the irradiation. These findings provide efficient preservation and sterilization methods for long-term usage of the fresh Tilapia skin grafts used for biological dressings.
    MeSH term(s) Animals ; Skin Transplantation ; Preservation, Biological ; Freeze Drying ; Collagen ; Fishes ; Sterilization/methods ; Ichthyosis, Lamellar
    Chemical Substances Collagen (9007-34-5)
    Language English
    Publishing date 2024-01-13
    Publishing country England
    Document type Journal Article
    ZDB-ID 2615211-3
    ISSN 2045-2322 ; 2045-2322
    ISSN (online) 2045-2322
    ISSN 2045-2322
    DOI 10.1038/s41598-024-51608-4
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article: In vitro expansion of human mesenchymal stem cells and hepatocytes using purified platelet derived growth factors, a potential therapeutic modality for liver fibrosis.

    Elsaied, Hanaa M / Elzallat, Mohamed / Yassin, Aisha / Elfedawy, Shahira / Soliman, Dina A / Ragab, Dina / Fahmy, Hossam M

    The Egyptian journal of immunology

    2023  Volume 30, Issue 2, Page(s) 47–58

    Abstract: Mesenchymal stem cells (MSCs) and hepatocytes are considered valuable candidates for cell-based therapy. The use of free zoonotic media, as purified platelets derived growth factors (L-GF) and human platelet lysate (hPL), instead of using fetal bovine ... ...

    Abstract Mesenchymal stem cells (MSCs) and hepatocytes are considered valuable candidates for cell-based therapy. The use of free zoonotic media, as purified platelets derived growth factors (L-GF) and human platelet lysate (hPL), instead of using fetal bovine serum (FBS) to support the growth and proliferation of these cells could be used as a promising therapeutic tool in hepatic regeneration. This study aimed to evaluate the usage of purified platelet derived growth factors and platelet lysate in both MSCs and hepatocyte cultures and to compare them with the usage of FBS. MSCs and hepatocytes were cultured in growth media supplemented with L-GF or hPL and compared them to their culture in growth media supplemented with FBS. Cells were subjected to population doubling (PD) and generation time (GT) calculations. The best result for MSCs was that obtained by using 10% hPL or 10% FBS with the highest cell count, highest viability and shortest incubation time needed to reach confluency compared to supplementation with 10%, 20% or 30% L-GF. As for hepatocyte culture, the use of 10% FBS for supplementation of media used for hepatic cell proliferation showed better performance regarding cell count, viability, and incubation time to reach confluency compared to the use of either hPL or L-GF. In conclusion, our study showed that 10% hPL had the best results in MSCs culture which suggests that hPL could be a better alternative for the development of xenofree stem cell culture that can be used for many clinical applications. On the other hand, 10% FBS showed the best results in hepatocyte culture.
    MeSH term(s) Humans ; Platelet-Derived Growth Factor/metabolism ; Cell Differentiation ; Mesenchymal Stem Cells ; Cells, Cultured ; Blood Platelets ; Culture Media/metabolism ; Liver Cirrhosis ; Hepatocytes
    Chemical Substances Platelet-Derived Growth Factor ; Culture Media
    Language English
    Publishing date 2023-04-06
    Publishing country Egypt
    Document type Journal Article
    ISSN 1110-4902
    ISSN 1110-4902
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article ; Online: Lyophilized equine platelet-rich plasma (L-GF

    Abdoon, Ahmed Sabry S / Al-Atrash, Ahmed M E / Soliman, Seham S / El-Sanea, Amro M / Gamal El Din, Amina A / Fahmy, Hossam M

    Journal of ovarian research

    2023  Volume 16, Issue 1, Page(s) 84

    Abstract: Background: The antineoplastic agent Cyclophosphamide (CP) induces reproductive toxicity. New strategies for protecting ovarian tissue damage in women with chemotherapy-induced reproductive toxicity are essential. This study was designed to evaluate the ...

    Abstract Background: The antineoplastic agent Cyclophosphamide (CP) induces reproductive toxicity. New strategies for protecting ovarian tissue damage in women with chemotherapy-induced reproductive toxicity are essential. This study was designed to evaluate the possible protective effect of combined treatment with L-GF
    Methodology: Forty mature female rats were assigned into four groups: First group, control: rats were intraperitoneally injected (IP) with 200 µl sterile saline solution on days 1 and 10; Group 2 (CP): were IP injected with 75 mg/kg on days 1 and 10 to induce POI); Group 3 (CP + L-GF
    Results: On day 21 of the experiment, body weight, ovarian parameters (Ovarian weight, uterine weight, the number of ovarian follicles, and corpora lutea (CL) were determined, and histopathological changes, blood profile, as well as antioxidant activity assessment, were performed. CP significantly suppresses ovarian and uterine functions and increased MAD, NO levels, RBCs, hemoglobin, WBCs, and platelet count compared to the control group ( P < 0.05). While, in CP + L-GF
    Conclusion: co-administration of L-GF
    MeSH term(s) Rats ; Female ; Horses ; Animals ; Rats, Wistar ; Cyclophosphamide/toxicity ; Oxidative Stress ; Antioxidants/pharmacology ; Antioxidants/metabolism ; Body Weight
    Chemical Substances Cyclophosphamide (8N3DW7272P) ; Antioxidants
    Language English
    Publishing date 2023-04-28
    Publishing country England
    Document type Journal Article
    ZDB-ID 2455679-8
    ISSN 1757-2215 ; 1757-2215
    ISSN (online) 1757-2215
    ISSN 1757-2215
    DOI 10.1186/s13048-023-01161-x
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article ; Online: Lyophilized growth factor intralesional injection in female pattern hair loss: A clinical and trichoscopic study.

    El Samahy, May H / Fahmy, Hossam M / El Sawaf, Samar I / Mostafa, Azza E

    Dermatologic therapy

    2021  Volume 34, Issue 2, Page(s) e14867

    Abstract: Various therapeutic modalities have been tried for female pattern hair loss (FPHL) treatment. To our knowledge, no previous studies had evaluated the therapeutic effect of lyophilized growth factor (L-GF) intralesional injection in FPHL. The current ... ...

    Abstract Various therapeutic modalities have been tried for female pattern hair loss (FPHL) treatment. To our knowledge, no previous studies had evaluated the therapeutic effect of lyophilized growth factor (L-GF) intralesional injection in FPHL. The current study aimed to evaluate the efficacy and safety of intralesional L-GF injection in FPHL by clinical and trichoscopic evaluation. This study included 20 patients with FPHL. All patients received three treatment sessions of intralesional injection of L-GF 4 weeks apart. Patients were followed-up for further 3 months. The outcome was evaluated by trichoscopy, photography score, patient's satisfaction score and side effects were reported. Trichoscopic evaluation showed significant posttreatment increase in all hair parameters associated with a significant decrease in vellus hair count. Ludwig's grade II showed posttreatment significant differences in all trichoscopic parameters from the baseline. No significant differences were detected regarding all trichoscopic parameters between the two Ludwig's grades posttreatment. 80% of patients showed photography score improvement that was significantly higher in Ludwig's grade II than in grade I. 100% of patients showed improvement in patient's satisfaction score with insignificant difference between Ludwig's grades. Intralesional injection of L-GF is safe and improved various trichoscopic hair parameters and clinical scores in FPHL.
    MeSH term(s) Alopecia/diagnosis ; Alopecia/drug therapy ; Female ; Hair ; Humans ; Injections, Intralesional ; Intercellular Signaling Peptides and Proteins/therapeutic use ; Photography
    Chemical Substances Intercellular Signaling Peptides and Proteins
    Language English
    Publishing date 2021-02-17
    Publishing country United States
    Document type Journal Article
    ZDB-ID 1354801-3
    ISSN 1529-8019 ; 1396-0296
    ISSN (online) 1529-8019
    ISSN 1396-0296
    DOI 10.1111/dth.14867
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 5181: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (2.OG)
    ab Jg. 2022: Lesesaal (EG)

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  5. Article: Intraovarian Injection of Reconstituted Lyophilized Growth-Promoting Factor Extracted from Horse Blood Platelets (L-GF

    Borş, Silviu-Ionuț / Dascălu, Dan-Lucian / Borş, Alina / Fahmy, Hossam M / Kandil, Omaima M / Abdoon, Ahmed Sabry S

    Animals : an open access journal from MDPI

    2022  Volume 12, Issue 19

    Abstract: The purpose of this study was to determine the impact of intraovarian injections of a reconstituted lyophilized growth-promoting factor extracted from horse blood platelets (L-GFequina) on the number of ovarian follicles, the recovery of cumulus−oocyte ... ...

    Abstract The purpose of this study was to determine the impact of intraovarian injections of a reconstituted lyophilized growth-promoting factor extracted from horse blood platelets (L-GFequina) on the number of ovarian follicles, the recovery of cumulus−oocyte complexes (COCs), and embryo development to the blastocyst stage in Holstein cows. Thus, 12 Holstein cows were assigned to three protocols. According to the number of punctured follicles in protocol 1, ovum pick-up (OPU) was conducted on days 6 and 14 of the cycle (day 0 = estrus). In protocol 2, every large follicle (more than 7 mm) was removed, and 1 mL of L-GFequina was intraovarian injected (day 0). Two days later, equine chorionic gonadotropin (eCG) was administered, and OPU sessions were conducted on days 6, 10, and 14. The same ovarian stimulation procedure as that in protocol 2 was performed in protocol 3, except that equine L-GFequina was not supplied. OPU was carried out on days 6 and 10 of the cycle. The results indicate that the intraovarian injection of L-GFequina significantly (p < 0.05) increased the number of OPU sessions per cycle, the recovery of cumulus−oocyte complexes (COCs), and the production of blastocysts. In conclusion, an intraovarian injection of L-GFequina can improves OPU-IVEP results in Holstein cows.
    Language English
    Publishing date 2022-09-29
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2606558-7
    ISSN 2076-2615
    ISSN 2076-2615
    DOI 10.3390/ani12192618
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  6. Article ; Online: Repair of experimentally induced femoral chondral defect in a rabbit model using Lyophilized growth promoting factor extracted from horse blood platelets (L-GF

    Abd El-Rahman, Sahar S / Amer, Mohammed S / Hassan, Marwa H / Fahmy, Hossam M / Shamaa, Ashraf A

    Injury

    2022  Volume 53, Issue 4, Page(s) 1375–1384

    Abstract: Lyophilized equine platelet derived growth factors (LGF) is a novel advanced platelet rich protein growth factor. It has been successfully applied in various fields of regenerative medicine to treat a variety of inflammatory and degenerative ... ...

    Abstract Lyophilized equine platelet derived growth factors (LGF) is a novel advanced platelet rich protein growth factor. It has been successfully applied in various fields of regenerative medicine to treat a variety of inflammatory and degenerative musculoskeletal conditions. Our study aimed to evaluate the efficacy of intraarticularly injected LGF for the remedy of articular cartilage injury, commonly characterized by progressive pain and loss of joint function in osteoarthritic rabbits. Full-thickness cylindrical cartilage defects were generated in both femoral condylar articular surfaces in twenty rabbits. The left joint of all animals was injected with the adjuvant as a self-control negative, while the right joint was injected by LGF. Four- and eight-weeks post-surgery, the femoral condyles were harvested, and assessed grossly, microscopically and immunohistochemically. Cytokines (TNF-α, IL-1β, PDGF and TGF-β1) contents of the chondral defects were quantified by ELISA as well as the gene expression of Col I and Col II via RT-qPCR. The LGF treated defects showed significant higher ICRS (International cartilage repair society) healing scores of cartilaginous regeneration with a significant higher histological healing score on using O'Driscoll histological scoring system. Additionally, LGF significantly lowered the levels of the pro-inflammatory cytokines TNF-α and IL-1β. It also significantly increased the anabolic and angiogenic growth factors (PDGF and TGF-β1), and significantly elevated the expression of chondrogenic-related marker genes; Col I and Col II. The current study reveals that LGF improves chondral healing and thus it can be a superior nominee as an adjunctive therapy to positively influence regeneration of chondral defects in osteoarthritic patients.
    MeSH term(s) Animals ; Blood Platelets ; Cartilage Diseases ; Cartilage, Articular/pathology ; Horses ; Humans ; Knee Joint ; Platelet-Derived Growth Factor ; Rabbits
    Chemical Substances Platelet-Derived Growth Factor
    Language English
    Publishing date 2022-02-05
    Publishing country Netherlands
    Document type Journal Article
    ZDB-ID 218778-4
    ISSN 1879-0267 ; 0020-1383
    ISSN (online) 1879-0267
    ISSN 0020-1383
    DOI 10.1016/j.injury.2022.02.004
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 778: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (1.OG)
    ab Jg. 2022: Lesesaal (EG)
    Zs.MO 10: Show issues

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  7. Book ; Conference proceedings ; Online: International Conference on Networking and Media Convergence, 2009

    Fahmy, Hossam M. A

    ICNM 2009 ; 24 - 25 March 2009, Information Network, Ain Shams University, Cairo, Egypt

    2009  

    Institution Ǧāmiʿat ʿAin Šams
    Event/congress ICNM (1, 2009.03.24-25, Cairo) ; International Conference on Networking and Media Convergence (1, 2009.03.24-25, Cairo)
    Author's details ed. by: Hossam M. A. Fahmy
    Language English
    Size Online-Ressource (127 S.)
    Publisher IEEE
    Publishing place Piscataway, NJ
    Document type Book ; Conference proceedings ; Online
    Note Parallel als Online-Ausg. erschienen
    ISBN 9781424437764 ; 9781424437771 ; 1424437768 ; 1424437776
    Database Former special subject collection: coastal and deep sea fishing

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  8. Book ; Conference proceedings: The 2009 International Conference on Networking and Media Convergence (ICNM '09)

    Fahmy, Hossam M. A

    March, 2009, Information Network, Ain Shams University, Cairo, Egypt

    2009  

    Institution Ǧāmiʿat ʿAin Šams
    Event/congress ICNM (1, 2009.03.24-25, Cairo) ; International Conference on Networking and Media Convergence (1, 2009.03.24-25, Cairo)
    Author's details ed. by: Hossam M. A. Fahmy
    Language English
    Size 127 S., graph. Darst.
    Publisher IEEE
    Publishing place Piscataway, NJ
    Document type Book ; Conference proceedings
    Note Parallel als Online-Ausg. erschienen
    ISBN 9781424437764 ; 9781424437771 ; 1424437768 ; 1424437776
    Database Library catalogue of the German National Library of Science and Technology (TIB), Hannover

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  9. Book ; Conference proceedings ; Online: International Conference on Networking and Media Convergence, 2009

    Fahmy, Hossam M. A

    ICNM 2009 ; 24 - 25 March 2009, Information Network, Ain Shams University, Cairo, Egypt

    2009  

    Institution Ǧāmiʿat ʿAin Šams
    Event/congress ICNM (1, 2009.03.24-25, Cairo) ; International Conference on Networking and Media Convergence (1, 2009.03.24-25, Cairo)
    Author's details ed. by: Hossam M. A. Fahmy
    Language English
    Size Online-Ressource (127 S.)
    Publisher IEEE
    Publishing place Piscataway, NJ
    Document type Book ; Conference proceedings ; Online
    Note Parallel als Online-Ausg. erschienen
    ISBN 9781424437764 ; 9781424437771 ; 1424437768 ; 1424437776
    Database Library catalogue of the German National Library of Science and Technology (TIB), Hannover

    Full text online

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  10. Book ; Conference proceedings: The 2008 International Conference on Computer Engineering & Systems (ICCES '08)

    Fahmy, Hossam M. A

    November 25 - 27, 2008, Cairo, Egypt

    2008  

    Institution Ǧāmiʿa / Computer Engineering and Systems Department
    Event/congress ICCES (2008.11.25-27, Cairo) ; International Conference on Computer Engineering & Systems (2008.11.25-27, Cairo)
    Author's details Faculty of Engineering, Ain Shams University, Computer Engineering & Systems Department. Ed. by: Hossam M. A. Fahmy
    Language English ; Arabic
    Size 389 S., Ill., graph. Darst.
    Publisher IEEE
    Publishing place Piscataway, NJ
    Document type Book ; Conference proceedings
    Note Titel auch in arab. Sprache
    ISBN 9781424421152 ; 1424421152
    Database Library catalogue of the German National Library of Science and Technology (TIB), Hannover

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