Article: Using MALDI-TOF MS to Identify Mosquitoes from Senegal and the Origin of Their Blood Meals.
2023 Volume 14, Issue 10
Abstract: Mosquitoes are arthropods that represent a real public health problem in Africa. Morphology and molecular biology techniques are usually used to identify different mosquito species. In recent years, an innovative tool, matrix-assisted desorption/ ... ...
| Abstract | Mosquitoes are arthropods that represent a real public health problem in Africa. Morphology and molecular biology techniques are usually used to identify different mosquito species. In recent years, an innovative tool, matrix-assisted desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS), has been used to identify many arthropods quickly and at low cost, where equipment is available. We evaluated the ability of MALDI-TOF MS to identify mosquitoes collected in Senegal and stored for several months in silica gel, and to determine the origin of their blood meal. A total of 582 mosquitoes were collected and analysed. We obtained 329/582 (56.52%) MALDI-TOF MS good-quality spectra from mosquito legs and 123/157 (78.34%) good-quality spectra from engorged abdomens. We updated our home-made MALDI-TOF MS arthropod spectra database by adding 23 spectra of five mosquito species from Senegal that had been identified morphologically and molecularly. These included legs from |
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| Language | English |
| Publishing date | 2023-09-26 |
| Publishing country | Switzerland |
| Document type | Journal Article |
| ZDB-ID | 2662247-6 |
| ISSN | 2075-4450 |
| ISSN | 2075-4450 |
| DOI | 10.3390/insects14100785 |
| Database | MEDical Literature Analysis and Retrieval System OnLINE |
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