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  1. Article ; Online: Expression and Purification of FGFR1-Fc Fusion Protein and Its Effects on Human Lung Squamous Carcinoma.

    Zheng, Lulu / Liu, Huan / Chen, Lingfeng / You, Xinyi / Lv, Fangyi / Fan, Haibing / Hui, Qi / Liu, Baohua / Wang, Xiaojie

    Applied biochemistry and biotechnology

    2023  Volume 196, Issue 1, Page(s) 573–587

    Abstract: Molecular-targeted therapies for lung squamous cell carcinoma (LSCC) are limited mainly because targetable oncogenic aberrations are absent in LSCC. Recent genomic analyses have revealed that the fibroblast growth factor (FGF) signaling pathway plays a ... ...

    Abstract Molecular-targeted therapies for lung squamous cell carcinoma (LSCC) are limited mainly because targetable oncogenic aberrations are absent in LSCC. Recent genomic analyses have revealed that the fibroblast growth factor (FGF) signaling pathway plays a fundamental role in LSCC progression via cancer cell proliferation and angiogenesis. In the present study, we designed, expressed, and purified a fibroblast growth factor receptor fragment (FGFR1-Fc) fusion protein using NS/0 cells. In FGF2-FGFR1 overexpressed NCI-H1703 cells, the FGFR1-Fc fusion protein effectively inhibited proliferation and invasion and arrested the cell cycle at the G0-G1 phase. In NCI-H1703 cells treated with the FGFR1-Fc fusion protein, the phosphorylation levels of FGFR1, FRS2, ERK, and AKT were significantly reduced. Using an siRNA assay, we demonstrated that FGF2-FGFR1 is the major anti-tumor target of FGFR1-Fc fusion the FGFR1-Fc fusion protein, which also significantly inhibited proliferation and invasion by NCI-H1703 cells via the FGF2-FGFR1 signaling pathway. In addition, the FGFR1-Fc fusion protein significantly inhibited angiogenesis in an embryonic chorioallantoic membrane model. The FGFR1-Fc fusion protein may be an effective therapeutic candidate for LSCC.
    MeSH term(s) Humans ; Fibroblast Growth Factor 2 ; Receptor, Fibroblast Growth Factor, Type 1/genetics ; Receptor, Fibroblast Growth Factor, Type 1/metabolism ; Carcinoma, Non-Small-Cell Lung ; Cell Proliferation ; Carcinoma, Squamous Cell/genetics ; Lung Neoplasms/drug therapy ; Lung/metabolism ; Cell Line, Tumor ; Immunoglobulin G ; Recombinant Fusion Proteins
    Chemical Substances FP-1039 (CTG26PRE5S) ; Fibroblast Growth Factor 2 (103107-01-3) ; Receptor, Fibroblast Growth Factor, Type 1 (EC 2.7.10.1) ; FGFR1 protein, human (EC 2.7.10.1) ; Immunoglobulin G ; Recombinant Fusion Proteins
    Language English
    Publishing date 2023-05-09
    Publishing country United States
    Document type Journal Article
    ZDB-ID 392344-7
    ISSN 1559-0291 ; 0273-2289
    ISSN (online) 1559-0291
    ISSN 0273-2289
    DOI 10.1007/s12010-023-04542-6
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 3053: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (2.OG)
    ab Jg. 2022: Lesesaal (EG)

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  2. Article: Toxicokinetics of recombinant human fibroblast growth factor 21 for injection in cynomolgus monkey for 3 months.

    Lu, Chao / Jin, Lei / Bi, Jianing / Jin, Hongyi / You, Xinyi / Peng, Lulu / Fan, Haibing / Wang, Huan / Wang, Liangshun / Fan, Zhengkai / Wang, Xiaojie / Liu, Baohua

    Frontiers in pharmacology

    2023  Volume 14, Page(s) 1176136

    Abstract: Introduction: ...

    Abstract Introduction:
    Language English
    Publishing date 2023-05-23
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2587355-6
    ISSN 1663-9812
    ISSN 1663-9812
    DOI 10.3389/fphar.2023.1176136
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article ; Online: rhFGF-21 accelerates corneal epithelial wound healing through the attenuation of oxidative stress and inflammatory mediators in diabetic mice.

    Li, Le / Wang, Huan / Pang, Shucai / Wang, Liangshun / Fan, Zhengkai / Ma, Chunyu / Yang, Shufen / Banda, Joshua / Hui, Qi / Lv, Fangyi / Fan, Haibing / Huang, Tongzhou / Zhang, Xiaobi / Wang, Xiaojie

    The Journal of biological chemistry

    2023  Volume 299, Issue 9, Page(s) 105127

    Abstract: Diabetic keratopathy, commonly associated with a hyperactive inflammatory response, is one of the most common eye complications of diabetes. The peptide hormone fibroblast growth factor-21 (FGF-21) has been demonstrated to have anti-inflammatory and ... ...

    Abstract Diabetic keratopathy, commonly associated with a hyperactive inflammatory response, is one of the most common eye complications of diabetes. The peptide hormone fibroblast growth factor-21 (FGF-21) has been demonstrated to have anti-inflammatory and antioxidant properties. However, whether administration of recombinant human (rh) FGF-21 can potentially regulate diabetic keratopathy is still unknown. Therefore, in this work, we investigated the role of rhFGF-21 in the modulation of corneal epithelial wound healing, the inflammation response, and oxidative stress using type 1 diabetic mice and high glucose-treated human corneal epithelial cells. Our experimental results indicated that the application of rhFGF-21 contributed to the enhancement of epithelial wound healing. This treatment also led to advancements in tear production and reduction in corneal edema. Moreover, there was a notable reduction in the levels of proinflammatory cytokines such as TNF-α, IL-6, IL-1β, MCP-1, IFN-γ, MMP-2, and MMP-9 in both diabetic mouse corneal epithelium and human corneal epithelial cells treated with high glucose. Furthermore, we found rhFGF-21 treatment inhibited reactive oxygen species production and increased levels of anti-inflammatory molecules IL-10 and SOD-1, which suggests that FGF-21 has a protective role in diabetic corneal epithelial healing by increasing the antioxidant capacity and reducing the release of inflammatory mediators and matrix metalloproteinases. Therefore, we propose that administration of FGF-21 may represent a potential treatment for diabetic keratopathy.
    MeSH term(s) Animals ; Humans ; Mice ; Anti-Inflammatory Agents/pharmacology ; Anti-Inflammatory Agents/therapeutic use ; Antioxidants/pharmacology ; Antioxidants/therapeutic use ; Corneal Diseases/complications ; Corneal Diseases/drug therapy ; Corneal Diseases/metabolism ; Diabetes Complications/drug therapy ; Diabetes Complications/metabolism ; Diabetes Mellitus, Experimental/complications ; Diabetes Mellitus, Experimental/drug therapy ; Diabetes Mellitus, Experimental/metabolism ; Epithelium, Corneal/drug effects ; Fibroblast Growth Factors/pharmacology ; Fibroblast Growth Factors/therapeutic use ; Glucose/adverse effects ; Glucose/metabolism ; Inflammation Mediators/metabolism ; Matrix Metalloproteinases/metabolism ; Oxidative Stress/drug effects ; Wound Healing/drug effects
    Chemical Substances Anti-Inflammatory Agents ; Antioxidants ; fibroblast growth factor 21 ; Fibroblast Growth Factors (62031-54-3) ; Glucose (IY9XDZ35W2) ; Inflammation Mediators ; Matrix Metalloproteinases (EC 3.4.24.-)
    Language English
    Publishing date 2023-08-04
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2997-x
    ISSN 1083-351X ; 0021-9258
    ISSN (online) 1083-351X
    ISSN 0021-9258
    DOI 10.1016/j.jbc.2023.105127
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Uc I Zs.108: Show issues Location:
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    bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular
    ab Jg. 2022: Lesesaal (EG)

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