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  1. Article: [Evaluation of brain age changes in patients with liver cirrhosis and hepatic encephalopathy with deep learning models based on structural magnetic resonance imaging].

    Li, F F / Zhang, X D / Lu, Z N / Chen, C / Xu, J H / Fan, L Z / Cheng, Y

    Zhonghua yi xue za zhi

    2024  Volume 104, Issue 4, Page(s) 269–275

    Abstract: Objective: ...

    Abstract Objective:
    MeSH term(s) Humans ; Hepatic Encephalopathy ; Cross-Sectional Studies ; Deep Learning ; Brain ; Liver Cirrhosis ; Magnetic Resonance Imaging
    Language Chinese
    Publishing date 2024-01-05
    Publishing country China
    Document type English Abstract ; Journal Article
    ZDB-ID 132513-9
    ISSN 0376-2491
    ISSN 0376-2491
    DOI 10.3760/cma.j.cn112137-20231011-00710
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article: Potential role of p53 on metallothionein induction in human epithelial breast cancer cells.

    Fan, L Z / Cherian, M G

    British journal of cancer

    2002  Volume 87, Issue 9, Page(s) 1019–1026

    Abstract: The expression and induction of metallothionein has been associated with protection against oxidative stress and apoptosis. This study examines the effect of tumour suppressor protein p53 on metallothionein expression following CdCl2 treatment in eight ... ...

    Abstract The expression and induction of metallothionein has been associated with protection against oxidative stress and apoptosis. This study examines the effect of tumour suppressor protein p53 on metallothionein expression following CdCl2 treatment in eight human epithelial breast cancer cell lines differing in p53 and oestrogen-receptor status. Cells were treated with 10 microM CdCl2 for 24 h and metallothionein protein levels were measured by cadmium binding assay. MCF7 cells which are p53-positive (p53+) and oestrogen-receptor-positive showed a large induction in metallothionein synthesis by 10.79+/-1.36-fold. Other breast cancer cell lines which are p53-negative (p53-) and oestrogen-receptor-negative or weakly oestrogen-receptor-positive showed a small induction ranging from 1.40+/-0.10 to 3.65+/-0.30-fold. RT-PCR analysis showed an induction of metallothionein mRNA in MCF7 cells by about 1.61+/-0.08-fold, while in HCC1806 cells (p53-, oestrogen-receptor-negative) by 1.11+/-0.13-fold, and in MDA-MB-231 (p53-, oestrogen-receptor-negative) by 1.25+/-0.06-fold. Metallothionein localisation was determined by immunohistochemical staining. Prior to metal treatment, metallothionein was localised mainly in the cytoplasm of MCF7 and MDA-MB-231 cells. After treatment with 10 microM CdCl2 for 24 h, MCF7 cells showed intense nuclear and cytoplasmic staining for metallothionein, while MDA-MB-231 cells showed staining in the cytoplasm with weak nuclear staining. Apoptosis induced by 10-40 microM CdCl2 at time points between 4 and 48 h was examined with TUNEL assay. In MCF7 cells, apoptosis increased with higher concentrations of CdCl2, it peaked at 6-8 h and appeared again at 48 h for all concentrations of CdCl2 tested. In MDA-MB-231 cells, apoptosis remained at low levels for 10-40 microM CdCl2 at all time points. Studies on cadmium uptake showed similar uptake and accumulation of cadmium at 8 and 24 h in all the cell lines. The data demonstrate that treatment of epithelial breast cancer cells with 10 microM CdCl2 for 24 h caused a greater induction of metallothionein protein and mRNA expression in p53+ and oestrogen-receptor-positive cells as compared to p53- and oestrogen-receptor-negative or weakly oestrogen-receptor-positive cells. This effect may be associated with the occurrence of apoptosis and suggests a role for p53 and oestrogen-receptor on the expression and induction of metallothionein in epithelial cells.
    MeSH term(s) Apoptosis/drug effects ; Breast Neoplasms/metabolism ; Cadmium Chloride/pharmacology ; Cell Survival ; Epithelial Cells/drug effects ; Epithelial Cells/metabolism ; Female ; Gene Expression Regulation, Neoplastic ; Humans ; Immunoenzyme Techniques ; In Situ Nick-End Labeling ; Metallothionein/biosynthesis ; Metallothionein/genetics ; RNA, Messenger/metabolism ; RNA, Neoplasm/metabolism ; Receptors, Estrogen/metabolism ; Reverse Transcriptase Polymerase Chain Reaction ; Transfection ; Tumor Cells, Cultured/drug effects ; Tumor Cells, Cultured/metabolism ; Tumor Suppressor Protein p53/physiology
    Chemical Substances RNA, Messenger ; RNA, Neoplasm ; Receptors, Estrogen ; Tumor Suppressor Protein p53 ; Metallothionein (9038-94-2) ; Cadmium Chloride (J6K4F9V3BA)
    Language English
    Publishing date 2002-10-21
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 80075-2
    ISSN 1532-1827 ; 0007-0920
    ISSN (online) 1532-1827
    ISSN 0007-0920
    DOI 10.1038/sj.bjc.6600549
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article: Fluorogold as a retrograde tracer used in combination with immunohistochemistry.

    Ju, G / Han, Z S / Fan, L Z

    Journal of neuroscience methods

    1989  Volume 29, Issue 1, Page(s) 69–72

    Abstract: The fluorescent dye Fluorogold has been recently introduced as a retrograde neuronal tracer that can be used in combination with immunohistochemistry. It has, however, lately been reported that immunohistochemical processes induced rapid photobleaching ... ...

    Abstract The fluorescent dye Fluorogold has been recently introduced as a retrograde neuronal tracer that can be used in combination with immunohistochemistry. It has, however, lately been reported that immunohistochemical processes induced rapid photobleaching of Fluorogold. In the present study we prove that it is the water in the sections, and not the immunohistochemical processing itself, that is responsible for the photobleaching. This problem is easily corrected by using a 29:1 or 19:1 glycerine-PBS buffer mounting medium.
    MeSH term(s) Animals ; Brain/cytology ; Brain/immunology ; Brain/metabolism ; Fluorescent Antibody Technique ; Fluorescent Dyes ; Immunohistochemistry/methods ; Rats ; Rats, Inbred Strains ; Stilbamidines
    Chemical Substances 2-hydroxy-4,4'-diamidinostilbene, methanesulfonate salt ; Fluorescent Dyes ; Stilbamidines
    Language English
    Publishing date 1989-07
    Publishing country Netherlands
    Document type Journal Article
    ZDB-ID 282721-9
    ISSN 1872-678X ; 0165-0270
    ISSN (online) 1872-678X
    ISSN 0165-0270
    DOI 10.1016/0165-0270(89)90109-x
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article: The glucose oxidase-DAB-nickel method in peroxidase histochemistry of the nervous system.

    Shu, S Y / Ju, G / Fan, L Z

    Neuroscience letters

    1988  Volume 85, Issue 2, Page(s) 169–171

    Abstract: A combination of the glucose oxidase-diaminobenzidine (DAB) method and the DAB-nickel method can successfully bring out details of immunoreactive structures in immunostained preparations. It is especially beneficial for visualizing fibers and terminals. ...

    Abstract A combination of the glucose oxidase-diaminobenzidine (DAB) method and the DAB-nickel method can successfully bring out details of immunoreactive structures in immunostained preparations. It is especially beneficial for visualizing fibers and terminals.
    MeSH term(s) 3,3'-Diaminobenzidine ; Animals ; Brain/cytology ; Brain/enzymology ; Brain Chemistry ; Glucose Oxidase/analysis ; Histocytochemistry/methods ; Nickel ; Rats ; Rats, Inbred Strains
    Chemical Substances 3,3'-Diaminobenzidine (2RV4T6KHQI) ; Nickel (7OV03QG267) ; Glucose Oxidase (EC 1.1.3.4)
    Language English
    Publishing date 1988-02-29
    Publishing country Ireland
    Document type Journal Article
    ZDB-ID 194929-9
    ISSN 1872-7972 ; 0304-3940
    ISSN (online) 1872-7972
    ISSN 0304-3940
    DOI 10.1016/0304-3940(88)90346-1
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article: [An analysis of 3,798 cases of esophageal cancer treated by radiation (author's transl)].

    Yin, W B / Zhang, L J / Yang, Z Y / Miao, Y J / Yu, Z H / Zhang, Z X / Zhang, C H / Wang, M L / Li, G H / Fan, L Z / Zhang, L N / Liu, Y Y / Jia, Y Q / Ku, X Z

    Zhonghua zhong liu za zhi [Chinese journal of oncology

    1980  Volume 2, Issue 3, Page(s) 216–221

    MeSH term(s) Adult ; Aged ; Esophageal Neoplasms/diagnostic imaging ; Esophageal Neoplasms/radiotherapy ; Female ; Humans ; Male ; Middle Aged ; Prognosis ; Radiography
    Language Chinese
    Publishing date 1980-09
    Publishing country China
    Document type Journal Article
    ZDB-ID 603424-x
    ISSN 0253-3766
    ISSN 0253-3766
    Database MEDical Literature Analysis and Retrieval System OnLINE

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