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Book ; Online: CharacterEval

Tu, Quan / Fan, Shilong / Tian, Zihang / Yan, Rui

A Chinese Benchmark for Role-Playing Conversational Agent Evaluation

2024

Abstract: Recently, the advent of large language models (LLMs) has revolutionized generative agents. Among them, Role-Playing Conversational Agents (RPCAs) attract considerable attention due to their ability to emotionally engage users. However, the absence of a ... ...

Abstract	Recently, the advent of large language models (LLMs) has revolutionized generative agents. Among them, Role-Playing Conversational Agents (RPCAs) attract considerable attention due to their ability to emotionally engage users. However, the absence of a comprehensive benchmark impedes progress in this field. To bridge this gap, we introduce CharacterEval, a Chinese benchmark for comprehensive RPCA assessment, complemented by a tailored high-quality dataset. The dataset comprises 1,785 multi-turn role-playing dialogues, encompassing 23,020 examples and featuring 77 characters derived from Chinese novels and scripts. It was carefully constructed, beginning with initial dialogue extraction via GPT-4, followed by rigorous human-led quality control, and enhanced with in-depth character profiles sourced from Baidu Baike. CharacterEval employs a multifaceted evaluation approach, encompassing thirteen targeted metrics on four dimensions. Comprehensive experiments on CharacterEval demonstrate that Chinese LLMs exhibit more promising capabilities than GPT-4 in Chinese role-playing conversation. Source code, data source and reward model will be publicly accessible at https://github.com/morecry/CharacterEval.
Keywords	Computer Science - Computation and Language
Subject code	006
Publishing date	2024-01-02
Publishing country	us
Document type	Book ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Stability of collagen heterotrimer with same charge pattern and different charged residue identities

Huang, Yujie / Lan, Jun / Wu, Chao / Zhang, Ruixue / Zheng, Hongning / Fan, Shilong / Xu, Fei

Biophysical Society Biophysical Journal. 2023 July, v. 122, no. 13 p.2686-2695

2023

Abstract: Salt bridges are important factors in maintaining the stability of proteins, and their contribution to protein folding has received much attention. Although the interaction energies, or stabilizing contributions, of individual salt bridges have been ... ...

Abstract	Salt bridges are important factors in maintaining the stability of proteins, and their contribution to protein folding has received much attention. Although the interaction energies, or stabilizing contributions, of individual salt bridges have been measured in various proteins, a systematic assessment of various types of salt bridges in a relatively uniform environment is still a valuable analysis. Here, we used a collagen heterotrimer as a host-guest platform to construct 48 heterotrimers with the same charge pattern. A variety of salt bridges were formed between the oppositely charged residues Lys, Arg, Asp, and Glu. The melting temperature (Tₘ) of the heterotrimers was measured with circular dichroism. The atomic structures of 10 salt bridges were shown in three x-ray crystals of heterotrimer. Molecular dynamics simulation based on the crystal structures indicated that strong, intermediate, and weak salt bridges have distinctive N-O distances. A linear regression model was used to predict the stability of heterotrimers with high accuracy (R² = 0.93). We developed an online database to help readers understand how a salt bridge stabilizes collagen. This work will help us better understand the stabilizing mechanism of salt bridges in collagen folding and provide a new strategy to design collagen heterotrimers.
Keywords	X-radiation ; circular dichroism spectroscopy ; collagen ; databases ; molecular dynamics ; regression analysis ; temperature
Language	English
Dates of publication	2023-07
Size	p. 2686-2695.
Publishing place	Elsevier Inc.
Document type	Article ; Online
Note	Pre-press version
ZDB-ID	218078-9
ISSN	1542-0086 ; 0006-3495
ISSN (online)	1542-0086
ISSN	0006-3495
DOI	10.1016/j.bpj.2023.05.023
Database	NAL-Catalogue (AGRICOLA)

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Article ; Online: Application of image super-resolution recognition and artificial intelligence system in repairing students' psychological education problems.

Li, Shulian / Jiang, Haibin / Ding, Zhiqiang / Fan, Shilong / Li, Nan / Li, Xv

Preventive medicine

2023 Volume 173, Page(s) 107590

Abstract: With the continuous development of society, people's life pressure is constantly increasing, and the mental health problems of college students are becoming increasingly prominent, bringing many challenges to their education and management. Universities ... ...

Abstract	With the continuous development of society, people's life pressure is constantly increasing, and the mental health problems of college students are becoming increasingly prominent, bringing many challenges to their education and management. Universities should not only cultivate students' theoretical and professional knowledge and practical skills, but also attach importance to their mental health and effectively implement psychological education. Therefore, it is very necessary to develop and design a simple and effective student psychological evaluation system. As a new form of ideological and political transformation in universities in the era of big data, online ideological and political work has potential development space. It is necessary to carry out mental health education in universities, fully utilize online education forms, and improve ability of universities to repair mental health problems. Based on this, this system designs and implements software for typical image resolution based recognition and artificial intelligence. The use of B/S architecture in the development and use of. net technology and web server technology will enable more students to connect and use different terminals. In addition, an algorithm for image super-resolution recognition was proposed, which uses clustering convolution to improve residual blocks, improves modeling ability by extracting features on a larger scale, reduces the number of parameters to improve model calculation efficiency, and enables mental health educators and managers to work better. This article combines image super-resolution recognition technology with artificial intelligence technology to apply it to the process of psychological education in universities, thereby promoting the development of problem repair applications.
MeSH term(s)	Humans ; Artificial Intelligence ; Educational Status ; Students ; Health Education ; Algorithms ; Universities
Language	English
Publishing date	2023-06-25
Publishing country	United States
Document type	Journal Article
ZDB-ID	184600-0
ISSN	1096-0260 ; 0091-7435
ISSN (online)	1096-0260
ISSN	0091-7435
DOI	10.1016/j.ypmed.2023.107590
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Article ; Online: Stability of collagen heterotrimer with same charge pattern and different charged residue identities.

Huang, Yujie / Lan, Jun / Wu, Chao / Zhang, Ruixue / Zheng, Hongning / Fan, Shilong / Xu, Fei

Biophysical journal

2023 Volume 122, Issue 13, Page(s) 2686–2695

Abstract	Salt bridges are important factors in maintaining the stability of proteins, and their contribution to protein folding has received much attention. Although the interaction energies, or stabilizing contributions, of individual salt bridges have been measured in various proteins, a systematic assessment of various types of salt bridges in a relatively uniform environment is still a valuable analysis. Here, we used a collagen heterotrimer as a host-guest platform to construct 48 heterotrimers with the same charge pattern. A variety of salt bridges were formed between the oppositely charged residues Lys, Arg, Asp, and Glu. The melting temperature (T
MeSH term(s)	Collagen/metabolism ; Circular Dichroism ; Molecular Dynamics Simulation ; Protein Folding ; Temperature ; Thermodynamics ; Salts/chemistry
Chemical Substances	Collagen (9007-34-5) ; Salts
Language	English
Publishing date	2023-05-23
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	218078-9
ISSN	1542-0086 ; 0006-3495
ISSN (online)	1542-0086
ISSN	0006-3495
DOI	10.1016/j.bpj.2023.05.023
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Article: Intratracheal Administration of Stem Cell Membrane-Cloaked Naringin-Loaded Biomimetic Nanoparticles Promotes Resolution of Acute Lung Injury.

Jin, Hua / Zhao, Yue / Yao, Yinlian / Fan, Shilong / Luo, Renxing / Shen, Xin / Wang, Yanyan / Pi, Jiang / Huang, Gonghua

Antioxidants (Basel, Switzerland)

2024 Volume 13, Issue 3

Abstract: Cytokine storm and ROS overproduction in the lung always lead to acute lung injury (ALI) and acute respiratory distress syndrome (ARDS) in a very short time. Effectively controlling cytokine storm release syndrome (CRS) and scavenging ROS are key to the ... ...

Abstract	Cytokine storm and ROS overproduction in the lung always lead to acute lung injury (ALI) and acute respiratory distress syndrome (ARDS) in a very short time. Effectively controlling cytokine storm release syndrome (CRS) and scavenging ROS are key to the prevention and treatment of ALI/ARDS. In this work, the naringin nanoparticles (Nar-NPs) were prepared by the emulsification and evaporation method; then, the mesenchymal stem cell membranes (CMs) were extracted and coated onto the surface of the Nar-NPs through the hand extrusion method to obtain the biomimetic CM@Nar-NPs. In vitro, the CM@Nar-NPs showed good dispersity, excellent biocompatibility, and biosafety. At the cellular level, the CM@Nar-NPs had excellent abilities to target inflamed macrophages and the capacity to scavenge ROS. In vivo imaging demonstrated that the CM@Nar-NPs could target and accumulate in the inflammatory lungs. In an ALI mouse model,
Language	English
Publishing date	2024-02-26
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2704216-9
ISSN	2076-3921
ISSN	2076-3921
DOI	10.3390/antiox13030282
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article: Structural basis of DNA recognition of tomato yellow leaf curl virus replication-associated protein

Wang, Chaonan / Fan, Shilong / Xu, Ning / Li, Zhihong / Zhang, Senyan / Zhu, Shuifang

International journal of biological macromolecules. 2022 Apr. 30, v. 205

2022

Abstract: Conserved and multifunctional Geminivirus Replication-associated Protein (Rep) specifically recognizes the replication origin and initiates viral DNA replication. We report the X-ray crystallography-based structures of two complexes containing the N- ... ...

Abstract	Conserved and multifunctional Geminivirus Replication-associated Protein (Rep) specifically recognizes the replication origin and initiates viral DNA replication. We report the X-ray crystallography-based structures of two complexes containing the N-terminal domain (5–117aa) of Tomato yellow leaf curl virus (TYLCV) Rep: the catalytically-dead Rep in complex with nonanucleotide ssDNA (Rep⁵–¹¹⁷ Y101F-ssDNA) as well as the catalytically-active phosphotyrosine covalent adduct (Rep⁵–¹¹⁷-ssDNA). These structures provide functional insight into the role of Rep in viral replication. Metal ions stabilize the DNA conformation by interacting with the phosphate group of adenine and thus promote formation of the catalytic center. Furthermore, we identified a compound that inhibits the binding of Rep to ssDNA and dsDNA and found that the addition of metal ions compromises the inhibitory effectiveness of this compound. This study demonstrates the mechanism of DNA recognition and cleavage process of viral Rep, emphasizing the role of metal ions.
Keywords	DNA conformation ; DNA replication ; Tomato yellow leaf curl virus ; X-radiation ; adenine ; phosphates ; replication origin ; virus replication
Language	English
Dates of publication	2022-0430
Size	p. 316-328.
Publishing place	Elsevier B.V.
Document type	Article
ZDB-ID	282732-3
ISSN	1879-0003 ; 0141-8130
ISSN (online)	1879-0003
ISSN	0141-8130
DOI	10.1016/j.ijbiomac.2022.02.106
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Article ; Online: The DNA Recognition Motif of GapR Has an Intrinsic DNA Binding Preference towards AT-rich DNA.

Huang, Qian / Duan, Bo / Qu, Zhi / Fan, Shilong / Xia, Bin

Molecules (Basel, Switzerland)

2021 Volume 26, Issue 19

Abstract: The nucleoid-associated protein GapR found ... ...

Abstract	The nucleoid-associated protein GapR found in
MeSH term(s)	Amino Acid Motifs ; Bacterial Proteins/chemistry ; Bacterial Proteins/genetics ; Bacterial Proteins/metabolism ; Binding Sites ; Caulobacter crescentus/chemistry ; Caulobacter crescentus/genetics ; Caulobacter crescentus/metabolism ; Crystallography, X-Ray ; DNA, Bacterial/chemistry ; DNA, Bacterial/metabolism ; Gene Expression Regulation, Bacterial ; Models, Molecular ; Mutation ; Protein Binding ; Protein Conformation ; Protein Multimerization ; Trans-Activators/chemistry ; Trans-Activators/genetics ; Trans-Activators/metabolism
Chemical Substances	Bacterial Proteins ; DNA, Bacterial ; Trans-Activators
Language	English
Publishing date	2021-09-24
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	1413402-0
ISSN	1420-3049 ; 1431-5165 ; 1420-3049
ISSN (online)	1420-3049
ISSN	1431-5165 ; 1420-3049
DOI	10.3390/molecules26195776
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Article ; Online: An experimental study on heat transfer performance of a pulsating heat pipe radiator for CPU heat dissipation

Shang Fumin / Yang Qingjing / Liu Chaoyue / Fan Shilong / Liu Jianhong

E3S Web of Conferences, Vol 165, p

2020 Volume 06035

Abstract: To meet the requirement of electronic heat dissipation with high heat flux, a kind of heat dissipation device using pulsating heat pipe (PHP) for CPU heat dissipation was put forward. The heat transfer performance and surface temperature distribution of ... ...

Abstract	To meet the requirement of electronic heat dissipation with high heat flux, a kind of heat dissipation device using pulsating heat pipe (PHP) for CPU heat dissipation was put forward. The heat transfer performance and surface temperature distribution of the radiator are analyzed by analyzing the wall temperature distribution and the distribution of the evaporator and condenser of the PHP. The experimental results show that the change of wind speed has obvious influence on the operation of the PHP radiator. The surface temperature distribution of the PHP radiator is very uniform, which is especially beneficial for CPU cooling. The heat transfer performance of the PHP is better, and the minimum average thermal resistance is 0.19 k/W. In addition, there is no drying phenomenon when the temperature reaches about 120 °C, which indicates that the pulsating heat pipe has a very high heat transfer limit.
Keywords	Environmental sciences ; GE1-350
Subject code	621
Language	English
Publishing date	2020-01-01T00:00:00Z
Publisher	EDP Sciences
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: A versatile artificial metalloenzyme scaffold enabling direct bioelectrocatalysis in solution.

Yang, Xiaoti / Wu, Wenjie / Chen, Xiling / Wu, Fei / Fan, Shilong / Yu, Ping / Mao, Lanqun

Science advances

2022 Volume 8, Issue 44, Page(s) eabo3315

Abstract: Artificial metalloenzymes (ArMs) are commonly designed with protein scaffolds containing buried coordination pockets to achieve substrate specificity and product selectivity for homogeneous reactions. However, their reactivities toward heterogeneous ... ...

Abstract	Artificial metalloenzymes (ArMs) are commonly designed with protein scaffolds containing buried coordination pockets to achieve substrate specificity and product selectivity for homogeneous reactions. However, their reactivities toward heterogeneous transformations are limited because interfacial electron transfers are hampered by the backbone shells. Here, we introduce bacterial small laccase (SLAC) as a new protein scaffold for constructing ArMs to directly catalyze electrochemical transformations. We use molecular dynamics simulation, x-ray crystallography, spectroscopy, and computation to illustrate the scaffold-directed assembly of an oxo-bridged dicobalt motif on protein surface. The resulting ArM in aqueous phase catalyzes electrochemical water oxidation without mediators or electrode modifications. Mechanistic investigation reveals the role of SLAC scaffold in defining the four-electron transfer pathway from water to oxygen. Furthermore, we demonstrate that SLAC-based ArMs implemented with Ni
Language	English
Publishing date	2022-11-02
Publishing country	United States
Document type	Journal Article
ZDB-ID	2810933-8
ISSN	2375-2548 ; 2375-2548
ISSN (online)	2375-2548
ISSN	2375-2548
DOI	10.1126/sciadv.abo3315
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: Structural Achievability of an NH-π Interaction between Gln and Phe in a Crystal Structure of a Collagen-like Peptide.

Zhang, Ruixue / Xu, You / Lan, Jun / Fan, Shilong / Huang, Jing / Xu, Fei

Biomolecules

2022 Volume 12, Issue 10

Abstract: NH-π interactions between polar and aromatic residues are well distributed in proteins whose stabilizing effects have been investigated in globular and fibrous proteins. In order to gain structural insights into side chain NH-π interactions, we solved a ... ...

Abstract	NH-π interactions between polar and aromatic residues are well distributed in proteins whose stabilizing effects have been investigated in globular and fibrous proteins. In order to gain structural insights into side chain NH-π interactions, we solved a crystal structure of a collagen-like peptide containing Gln-Phe pairs. The Gln-Phe NH-π interactions were further characterized by quantum calculations, molecular simulations, and structural bioinformatics. The analyses indicated that the NH-π interactions are robust under various solvent conditions, can be distributed either on the protein surface or in its hydrophobic core and can form at a wide range of distances between residues. This study suggested that NH-π interactions can play a versatile role in protein design, including engineering hydrophobic cores, solvent accessible surfaces, and protein-protein interfaces.
MeSH term(s)	Peptides/chemistry ; Collagen ; Hydrophobic and Hydrophilic Interactions ; Solvents
Chemical Substances	Peptides ; Collagen (9007-34-5) ; Solvents
Language	English
Publishing date	2022-10-06
Publishing country	Switzerland
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2701262-1
ISSN	2218-273X ; 2218-273X
ISSN (online)	2218-273X
ISSN	2218-273X
DOI	10.3390/biom12101433
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