LIVIVO - Search results -

Search results

Result 1 - 10 of total 118

Search options

Article: β-Sitosterol Reduces the Content of Triglyceride and Cholesterol in a High-Fat Diet-Induced Non-Alcoholic Fatty Liver Disease Zebrafish (

Zhang, Peng / Liu, Naicheng / Xue, Mingyang / Zhang, Mengjie / Xiao, Zidong / Xu, Chen / Fan, Yuding / Qiu, Junqiang / Zhang, Qinghua / Zhou, Yong

Animals : an open access journal from MDPI

2024 Volume 14, Issue 9

Abstract: Objective: Non-alcoholic fatty liver disease (NAFLD) is strongly associated with hyperlipidemia, which is closely related to high levels of sugar and fat. β-sitosterol is a natural product with significant hypolipidemic and cholesterol-lowering effects. ...

Abstract	Objective: Non-alcoholic fatty liver disease (NAFLD) is strongly associated with hyperlipidemia, which is closely related to high levels of sugar and fat. β-sitosterol is a natural product with significant hypolipidemic and cholesterol-lowering effects. However, the underlying mechanism of its action on aquatic products is not completely understood. Methods: A high-fat diet (HFD)-induced NAFLD zebrafish model was successfully established, and the anti-hyperlipidemic effect and potential mechanism of β-sitosterol were studied using oil red O staining, filipin staining, and lipid metabolomics. Results: β-sitosterol significantly reduced the accumulation of triglyceride, glucose, and cholesterol in the zebrafish model. Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis showed that differential lipid molecules in β-sitosterol mainly regulated the lipid metabolism and signal transduction function of the zebrafish model. β-sitosterol mainly affected steroid biosynthesis and steroid hormone biosynthesis in the zebrafish model. Compared with the HFD group, the addition of 500 mg/100 g of β-sitosterol significantly inhibited the expression of Conclusions: β-sitosterol can reduce lipid accumulation in the zebrafish model of NAFLD by regulating lipid metabolism and signal transduction and inhibiting adipogenesis and lipid storage.
Language	English
Publishing date	2024-04-25
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2606558-7
ISSN	2076-2615
ISSN	2076-2615
DOI	10.3390/ani14091289
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

Article ; Online: Isolation, identification and characterization of Aeromonas jandaei from diseased Chinese soft-shell turtles.

Chen, Mengmeng / Xue, Mingyang / Chen, Jingtao / Xiao, Zidong / Hu, Xiaowei / Zhang, Chunjie / Jiang, Nan / Fan, Yuding / Meng, Yan / Zhou, Yong

Journal of fish diseases

2024 Volume 47, Issue 4, Page(s) e13919

Abstract: Aeromonas jandaei is a gram-negative bacterium commonly found in aquatic environments and can induce illnesses in amphibians, reptiles and aquatic animals. In this study, a strain of bacteria was isolated from the diseased Chinese soft-shell turtle ( ... ...

Abstract	Aeromonas jandaei is a gram-negative bacterium commonly found in aquatic environments and can induce illnesses in amphibians, reptiles and aquatic animals. In this study, a strain of bacteria was isolated from the diseased Chinese soft-shell turtle (Pelodiscus sinensis), then named strain JDP-FX. This isolate was identified as A. jandaei after analysis of morphological, physiological and biochemical characteristics, as well as 16S rRNA and gyrB gene sequences. Virulence genetic testing further detected temperature-sensitive protease (eprCAI), type III secretion system (TTSS) (ascv), nuclease (nuc), cytotonic enterotoxin (alt) and serine proteinase (ser) in JDP-FX. Compared with healthy Chinese soft-shell turtle, the serum levels of total protein (TP), albumin (ALB) and globulin (GLB) were significantly decreased in the diseased Chinese soft-shell turtle, while, the levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST) and alkaline phosphatase (ALP) were significantly increased. Histopathological observations showed that multiple tissues, including intestinal mucosa, liver and kidney, were severely damaged in the diseased Chinese soft-shell turtle. Moreover, the diseased Chinese soft-shell turtle had significant cell degeneration, necrosis, sloughing and interstitial inflammatory cell infiltration. The pathogenicity of JDP-FX was tested via artificial infection. The median lethal dosage (LD
MeSH term(s)	Animals ; Turtles/genetics ; Turtles/metabolism ; RNA, Ribosomal, 16S/genetics ; RNA, Ribosomal, 16S/metabolism ; Fish Diseases ; China ; Aeromonas
Chemical Substances	RNA, Ribosomal, 16S
Language	English
Publishing date	2024-01-13
Publishing country	England
Document type	Journal Article
ZDB-ID	432109-1
ISSN	1365-2761 ; 0140-7775
ISSN (online)	1365-2761
ISSN	0140-7775
DOI	10.1111/jfd.13919
Database	MEDical Literature Analysis and Retrieval System OnLINE

Full text online

Accessible to users with ZB MED library card

In stock of ZB MED Bonn / Germany

Z 4276: Show issues

Order via subito

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Details ▾

Article ; Online: Isolation and identification of Chryseobacterium indologenes and its pathological changes in Pelodiscus sinensis

Xing, Yangyang / Xue, Mingyang / Xiao, Zidong / Hu, Xiaowei / Zhai, Jiale / Zhang, Chunjie / Jiang, Nan / Fan, Yuding / Meng, Yan / Zhou, Yong

Journal of Fish Diseases. 2024 Jan., v. 47, no. 1 p.e13864-

2024

Abstract: The Chinese revered a species of aquatic reptile known as Pelodiscus sinensis as both an edible and medicinal species. When artificially breeding, many deaths occurred at the farmed P. sinensis, mainly due to excessive breeding density, water ... ...

Abstract	The Chinese revered a species of aquatic reptile known as Pelodiscus sinensis as both an edible and medicinal species. When artificially breeding, many deaths occurred at the farmed P. sinensis, mainly due to excessive breeding density, water contamination, and turtles biting each other secondary to bacterial infections. In this study, an isolate of gram‐negative bacteria WH0623 was isolated from the liver and kidney of diseased P. sinensis to trace the potential pathogen of this disease. Based on biochemical characteristics and 16S rRNA gene sequencing analyses, this isolated strain of WH0623 was identified as Chryseobacterium indologenes. The strain's median lethal dose (LD₅₀) was 3.3 × 10⁵ colony‐forming units (CFU)/g per fish weight tested using artificial infection. Histopathological analysis revealed pathological changes, including cell swelling, hyperaemia, and necrosis in many tissues. Antibiotic susceptibility tests revealed that the bacteria WH0623 was susceptible to doxycycline, sulphonamides, ceftazidime, norfloxacin, and ciprofloxacin. These antibiotics could treat the disease. In conclusion, the pathogen causing the death of farmed P. sinensis was isolated and identified, and a drug‐sensitive test was conducted. Our findings contribute to the future diagnosis and treatment of the disease.
Keywords	Chryseobacterium indologenes ; Pelodiscus sinensis ; antibiotic resistance ; ceftazidime ; ciprofloxacin ; death ; doxycycline ; fish ; genes ; histopathology ; hyperemia ; kidneys ; lethal dose 50 ; liver ; necrosis ; norfloxacin ; pathogens ; reptiles ; water pollution
Language	English
Dates of publication	2024-01
Publishing place	John Wiley & Sons, Ltd
Document type	Article ; Online
Note	JOURNAL ARTICLE
ZDB-ID	432109-1
ISSN	1365-2761 ; 0140-7775
ISSN (online)	1365-2761
ISSN	0140-7775
DOI	10.1111/jfd.13864
Database	NAL-Catalogue (AGRICOLA)

Full text online

Accessible to users with ZB MED library card

In stock of ZB MED Bonn / Germany

Z 4276: Show issues

Order via subito

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Details ▾

Article ; Online: Development of a droplet digital PCR method for the sensitive detection and quantification of largemouth bass ranavirus

Jiang, Nan / Shen, Jinyu / Zhou, Yong / Liu, Wenzhi / Meng, Yan / Li, Yiqun / Xue, Mingyang / Xu, Chen / Fan, Yuding

Journal of Fish Diseases. 2023 Feb., v. 46, no. 2 p.91-98

2023

Abstract: Largemouth bass ranavirus (LMBRaV), also known as largemouth bass virus (LMBV), is a high mortality pathogen in largemouth bass. A rapid, sensitive, specific and convenient diagnosis method is an urgent requirement for the prevention of virus ... ...

Abstract	Largemouth bass ranavirus (LMBRaV), also known as largemouth bass virus (LMBV), is a high mortality pathogen in largemouth bass. A rapid, sensitive, specific and convenient diagnosis method is an urgent requirement for the prevention of virus transmission. In the present study, a droplet digital PCR (ddPCR) method based on the major capsid protein (mcp) gene was established to detect and quantify the virus genome copy number. Oligonucleotide primers were designed based on the LMBRaV mcp gene sequence. The specificity and sensitivity of ddPCR assay were analysed. The other aquatic virus including Chinese giant salamander iridovirus (GSIV), Cyprinid herpesvirus II (CyHV‐2) and infectious spleen and kidney necrosis virus could not be detected by LMBRaV ddPCR assay. The detection limit of ddPCR assay was 2 ± 0.37 copies/μl DNA sample. And this ddPCR assay had great repeatability and reproducibility. In clinical diagnosis of 50 largemouth bass, 43 positive samples were detected by ddPCR, whereas only 34 positive samples were detected by quantitative PCR (qPCR). This LMBRaV detection assay provided a specific and sensitive method for the rapid diagnosis of LMBRaV infection in largemouth bass as well as quantification of the virus load.
Keywords	Andrias davidianus ; Cyprinidae ; DNA ; Herpesvirales ; Infectious spleen and kidney necrosis virus ; Iridovirus ; Micropterus salmoides ; Santee-Cooper ranavirus ; coat proteins ; detection limit ; droplets ; fish ; genes ; mortality ; nucleotide sequences ; oligonucleotides ; pathogens ; quantitative polymerase chain reaction ; viral genome ; viral load ; virus transmission ; viruses
Language	English
Dates of publication	2023-02
Size	p. 91-98.
Publishing place	John Wiley & Sons, Ltd
Document type	Article ; Online
Note	JOURNAL ARTICLE
ZDB-ID	432109-1
ISSN	1365-2761 ; 0140-7775
ISSN (online)	1365-2761
ISSN	0140-7775
DOI	10.1111/jfd.13721
Database	NAL-Catalogue (AGRICOLA)

Full text online

Accessible to users with ZB MED library card

In stock of ZB MED Bonn / Germany

Z 4276: Show issues

Order via subito

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Details ▾

Article: Effects of Dietary Enterococcus faecalis YFI-G720 on the Growth, Immunity, Serum Biochemical, Intestinal Morphology, Intestinal Microbiota, and Disease Resistance of Crucian Carp (Carassius auratus)

Xu, Yan / Li, Yiqun / Xue, Mingyang / Xiao, Zidong / Fan, Yuding / Zeng, Lingbing / Zhou, Yong

Fishes. 2022 Jan. 12, v. 7, no. 1

2022

Abstract: Diseases of crucian carp (Carassius auratus) are closely related to intestinal parameters. Enterococcus faecalis has strong colonization ability in the intestinal tract, and produces natural antibiotics, bacteriocin, and other bacteriostatic substances, ... ...

Abstract	Diseases of crucian carp (Carassius auratus) are closely related to intestinal parameters. Enterococcus faecalis has strong colonization ability in the intestinal tract, and produces natural antibiotics, bacteriocin, and other bacteriostatic substances, which can effectively inhibit some pathogenic bacteria and improve the intestinal microenvironment. This study aimed to assess the effects of E. faecalis YFI-G720 which was isolated from the intestinal of crucian carp on the growth, immunity, intestinal health, and disease resistance of crucian carp. Fish (48.16 ± 0.55 g) were fed four diets, commercial diet or diet containing E. faecalis at 10⁵ CFU/g (EF1), 10⁶ CFU/g (EF2), or 10⁷ CFU/g (EF3) for 28 days. The results showed that supplementation of E. faecalis significantly improved the weight gain ratio (WGR) and the specific growth rate (SGR) compared with control group (p < 0.05). Intestinal mucosal epithelial cells in EF2 were intact and normal, but there was obvious vacuolation in CG. Compared with CG, serum C3 and IgM in EF2 were significantly increased at the end of the experiment (p < 0.05), and serum alkaline phosphatase was significantly higher in all experimental groups (p < 0.05). Among studied immune-related genes, expression was detected by qPCR, C3, IgM, and IL-1βwere upregulated in all experimental groups to varying degrees from 14 days, with highest expression in EF2 at 28 days. Intestinal microbiota structure analyzed through high-throughput sequencing, and the results showed that the relative abundance of Aeromonas and Acinetobacter decreased while Cetobacterium increased in all experimental groups, with the greatest changes in EF2. Challenge tests showed that fish fed E. faecalis were more resistant to Aeromonas veronii (p < 0.05). In conclusion, dietary E. faecalis YFI-G720 at 10⁶ CFU/g can improve the health status, immune parameters, intestinal microbiota composition, and disease resistance of crucian carp.
Keywords	Acinetobacter ; Aeromonas veronii ; Carassius auratus ; Enterococcus faecalis ; alkaline phosphatase ; antibacterial properties ; bacteriocins ; blood serum ; colonizing ability ; diet ; disease resistance ; epithelium ; fish ; health status ; immunity ; intestinal microorganisms ; intestines ; specific growth rate ; weight gain
Language	English
Dates of publication	2022-0112
Publishing place	Multidisciplinary Digital Publishing Institute
Document type	Article
ISSN	2410-3888
DOI	10.3390/fishes7010018
Database	NAL-Catalogue (AGRICOLA)

Order via subito

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Article: Characterization, expression pattern and antiviral activities of oligoadenylate synthetase in Chinese Giant Salamander, Andrias davidianus

Zhang, Jingjing / Li, Yiqun / Zhou, Yong / Jiang, Nan / Fan, Yuding / Lin, Ge / Zeng, Lingbing

Developmental and comparative immunology. 2022 Apr., v. 129

2022

Abstract: The enzyme 2′-5′-oligoadenylate synthetase (OAS) is an antiviral protein induced by interferons (IFNs), which plays an important role in IFN-mediated antiviral signaling pathway. In this study, the OAS of Chinese Giant Salamander, Andrias davidianus ( ... ...

Abstract	The enzyme 2′-5′-oligoadenylate synthetase (OAS) is an antiviral protein induced by interferons (IFNs), which plays an important role in IFN-mediated antiviral signaling pathway. In this study, the OAS of Chinese Giant Salamander, Andrias davidianus (AdOAS) was identified for the first time, and the expression profiles in vivo and the antiviral activities in vitro were investigated. The open reading frame (ORF) of AdOAS gene is 1185 bp in length, encoding a putative protein of 394 amino acids, in which a Nucleotidyltransferase (NTase) domain (40–143 aa) and a conserved OAS1 C superfamily domain (165–341 aa) are included. qRT-PCR analysis revealed a broad expression of AdOAS in vivo, with the highest expression level in intestine and heart. After infection with Chinese giant salamander iridovirus (GSIV), the mRNA level of AdOAS in liver increased significantly at 24 h and 48 h post infection and reached the peak at 72 h compared with the control group. The AdOAS mRNA level in kidney increased slightly at 6 h and 12 h post infection, declined to the initial level at 24 h and peaked at 48 h post infection, while in spleen it was slightly up-regulated at 6 h, inhibited at 12 h, 24 h and 48 h, and then significantly increased to the peak at 72 h post infection. In vitro, AdOAS mRNA level in Chinese giant salamander muscle (GSM) cells was not noticeably up-regulated until 24 h and then peaked at 48 h post GSIV infection. In antiviral activity test, the mRNA transcription and protein level of virus major capsid protein (MCP) in AdOAS over-expressed cells was significantly reduced compared with that in control cells by qRT-PCR and western blot analysis. In addition, ddPCR results showed that lower MCP gene copy was found in AdOAS over-expressed cells compared with the control group. These results collectively suggest that AdOAS plays a crucial role against GSIV infection in Chinese giant salamander, and provide a solid base for the further studies on the mechanism of immune defense and the control of the disease in this animal.
Keywords	Andrias davidianus ; Iridovirus ; Western blotting ; animals ; antiviral properties ; antiviral proteins ; coat proteins ; genes ; heart ; immune response ; intestines ; kidneys ; liver ; muscles ; protein content ; spleen ; viruses
Language	English
Dates of publication	2022-04
Publishing place	Elsevier Ltd
Document type	Article
ZDB-ID	752411-0
ISSN	1879-0089 ; 0145-305X
ISSN (online)	1879-0089
ISSN	0145-305X
DOI	10.1016/j.dci.2022.104347
Database	NAL-Catalogue (AGRICOLA)

Full text online

Accessible to users with ZB MED library card

In stock of ZB MED Cologne/Königswinter

Zs.A 1327: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
Jg. 1995 - 2021: Lesesall (1.OG)
ab Jg. 2022: Lesesaal (EG)

Order via subito

Details ▾

Article: Effects of tank diameter and water depth on growth performance, antioxidant capacity and immune response of Chinese sturgeon Acipenser sinensis in recirculating aquaculture system

Zhang, Haigeng / Jiang, Nan / Zhang, Yulei / Zhang, Yalei / Ni, Qi / Fan, Yuding

Journal of applied ichthyology. 2022 Jan., v. 38, no. 1

2022

Abstract: Chinese sturgeon, Acipenser sinensis, is one of the endangered aquatic species and have been successfully bred and artificially produced in China. To analyze the role of aquaculture habitat conditions in growth of Chinese sturgeon, the growth performance, ...

Abstract	Chinese sturgeon, Acipenser sinensis, is one of the endangered aquatic species and have been successfully bred and artificially produced in China. To analyze the role of aquaculture habitat conditions in growth of Chinese sturgeon, the growth performance, antioxidant capacity and immune responses of Chinese sturgeons under different tanks in the recirculating aquaculture system (RAS) were investigated. The fish (avg.wt. 2.83 ± 0.24 kg [SD]) were divided into two groups, one group were reared under three different water depths of tank (0.6, 1, 1.4 m) and the other group were under three different diameters of tank (2.5, 3.8, 7.5 m), which were named low‐depth (LD), medium‐depth (MD), high‐depth (HD), small‐diameter (SS), medium‐diameter (MS) and large‐diameter (LS), respectively. The results showed that at a depth of 0.6 m, the specific growth rate (SGR), weight gain rate (WG), serum glutathione peroxidase (GPX), alkaline phosphatase (AKP) and lysozyme (LZM) activities of Chinese sturgeons were significantly lower than those of other treatments (p < .05), while the food conversion ratio and malondialdehyde (MDA) showed a significant increased in this treatment (p < .05). In additional, when the fish in 2.5 m diameter tank treatment, the SGR, WG and the serum GPX, AKP, LZM indicator were significantly lower than the rest of the treatment (p < .05), while the serum MDA were significantly higher than the other treatment (p < .05). These results suggested that tank diameter and water depth had an influence on growth and therefore should be considered in RAS systems for Chinese sturgeon.
Keywords	Acipenser sinensis ; alkaline phosphatase ; antioxidant activity ; blood serum ; food conversion ; glutathione peroxidase ; growth performance ; habitats ; ichthyology ; immune response ; lysozyme ; malondialdehyde ; specific growth rate ; weight gain ; China
Language	English
Dates of publication	2022-01
Size	p. 84-92.
Publishing place	John Wiley & Sons, Ltd
Document type	Article
Note	JOURNAL ARTICLE
ZDB-ID	283875-8
ISSN	0175-8659
ISSN	0175-8659
DOI	10.1111/jai.14285
Database	NAL-Catalogue (AGRICOLA)

Full text online

Accessible to users with ZB MED library card

In stock of ZB MED Bonn / Germany

Z 4247: Show issues

Order via subito

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Details ▾

Article ; Online: Glycosylphosphatidylinositol Mannosyltransferase Ⅰ Protects Chinese Giant Salamander,

Zhang, Jingjing / Dai, Yanlin / Fan, Yuding / Jiang, Nan / Zhou, Yong / Zeng, Lingbing / Li, Yiqun

International journal of molecular sciences

2022 Volume 23, Issue 16

Abstract: Glycosylphosphatidylinositol mannosyltransferase I (GPI-MT-I) is an essential glycosyltransferase of glycosylphosphatidylinositol-anchor proteins (GPI-APs) that transfers the first of the four mannoses in GPI-AP precursors, which have multiple functions, ...

Abstract	Glycosylphosphatidylinositol mannosyltransferase I (GPI-MT-I) is an essential glycosyltransferase of glycosylphosphatidylinositol-anchor proteins (GPI-APs) that transfers the first of the four mannoses in GPI-AP precursors, which have multiple functions, including immune response and signal transduction. In this study, the GPI-MT-I gene that regulates GPI-AP biosynthesis in
MeSH term(s)	Animals ; China ; Iridovirus/genetics ; Iridovirus/metabolism ; Mannosyltransferases ; RNA, Messenger/genetics ; RNA, Messenger/metabolism ; Urodela
Chemical Substances	RNA, Messenger ; Mannosyltransferases (EC 2.4.1.-) ; glycosylphosphatidylinositol mannosyltransferase I (EC 2.4.1.-)
Language	English
Publishing date	2022-08-12
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2019364-6
ISSN	1422-0067 ; 1422-0067 ; 1661-6596
ISSN (online)	1422-0067
ISSN	1422-0067 ; 1661-6596
DOI	10.3390/ijms23169009
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Article ; Online: Characterization, expression pattern and antiviral activities of oligoadenylate synthetase in Chinese Giant Salamander, Andrias davidianus.

Zhang, Jingjing / Li, Yiqun / Zhou, Yong / Jiang, Nan / Fan, Yuding / Lin, Ge / Zeng, Lingbing

Developmental and comparative immunology

2022 Volume 129, Page(s) 104347

Abstract: The enzyme 2'-5'-oligoadenylate synthetase (OAS) is an antiviral protein induced by interferons (IFNs), which plays an important role in IFN-mediated antiviral signaling pathway. In this study, the OAS of Chinese Giant Salamander, Andrias davidianus ( ... ...

Abstract	The enzyme 2'-5'-oligoadenylate synthetase (OAS) is an antiviral protein induced by interferons (IFNs), which plays an important role in IFN-mediated antiviral signaling pathway. In this study, the OAS of Chinese Giant Salamander, Andrias davidianus (AdOAS) was identified for the first time, and the expression profiles in vivo and the antiviral activities in vitro were investigated. The open reading frame (ORF) of AdOAS gene is 1185 bp in length, encoding a putative protein of 394 amino acids, in which a Nucleotidyltransferase (NTase) domain (40-143 aa) and a conserved OAS1 C superfamily domain (165-341 aa) are included. qRT-PCR analysis revealed a broad expression of AdOAS in vivo, with the highest expression level in intestine and heart. After infection with Chinese giant salamander iridovirus (GSIV), the mRNA level of AdOAS in liver increased significantly at 24 h and 48 h post infection and reached the peak at 72 h compared with the control group. The AdOAS mRNA level in kidney increased slightly at 6 h and 12 h post infection, declined to the initial level at 24 h and peaked at 48 h post infection, while in spleen it was slightly up-regulated at 6 h, inhibited at 12 h, 24 h and 48 h, and then significantly increased to the peak at 72 h post infection. In vitro, AdOAS mRNA level in Chinese giant salamander muscle (GSM) cells was not noticeably up-regulated until 24 h and then peaked at 48 h post GSIV infection. In antiviral activity test, the mRNA transcription and protein level of virus major capsid protein (MCP) in AdOAS over-expressed cells was significantly reduced compared with that in control cells by qRT-PCR and western blot analysis. In addition, ddPCR results showed that lower MCP gene copy was found in AdOAS over-expressed cells compared with the control group. These results collectively suggest that AdOAS plays a crucial role against GSIV infection in Chinese giant salamander, and provide a solid base for the further studies on the mechanism of immune defense and the control of the disease in this animal.
MeSH term(s)	Adenine Nucleotides ; Amino Acid Sequence ; Amphibian Proteins/genetics ; Animals ; Antiviral Agents/metabolism ; Apoptosis ; Cell Line ; China ; Interferons/metabolism ; Iridovirus/physiology ; Kidney/metabolism ; Ligases/genetics ; Ligases/metabolism ; Oligoribonucleotides ; Open Reading Frames ; Signal Transduction/genetics ; Spleen/metabolism ; Urodela/genetics
Chemical Substances	Adenine Nucleotides ; Amphibian Proteins ; Antiviral Agents ; Oligoribonucleotides ; 2',5'-oligoadenylate (61172-40-5) ; Interferons (9008-11-1) ; Ligases (EC 6.-)
Language	English
Publishing date	2022-01-08
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	752411-0
ISSN	1879-0089 ; 0145-305X
ISSN (online)	1879-0089
ISSN	0145-305X
DOI	10.1016/j.dci.2022.104347
Database	MEDical Literature Analysis and Retrieval System OnLINE

Full text online

Accessible to users with ZB MED library card

In stock of ZB MED Cologne/Königswinter

Zs.A 1327: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
Jg. 1995 - 2021: Lesesall (1.OG)
ab Jg. 2022: Lesesaal (EG)

Order via subito

Details ▾

Article ; Online: The probiotic effects of host-associated

Ji, Zhehui / Lu, Xing / Xue, Mingyang / Fan, Yuding / Tian, Juan / Dong, Lixue / Zhu, Chuanzhong / Wen, Hua / Jiang, Ming

Animal nutrition (Zhongguo xu mu shou yi xue hui)

2023 Volume 15, Page(s) 114–125

Abstract: This study was to evaluate the potential of a host- ... ...

Abstract	This study was to evaluate the potential of a host-associated
Language	English
Publishing date	2023-08-22
Publishing country	China
Document type	Journal Article
ISSN	2405-6383
ISSN (online)	2405-6383
DOI	10.1016/j.aninu.2023.08.004
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

To top

More links

Kategorien

Order via subito

Full text online

More links

Kategorien

In stock of ZB MED Bonn / Germany

Order via subito

Inter-library loan at ZB MED

Full text online

More links

Kategorien

In stock of ZB MED Bonn / Germany

Order via subito

Inter-library loan at ZB MED

Full text online

More links

Kategorien

In stock of ZB MED Bonn / Germany

Order via subito

Inter-library loan at ZB MED

More links

Kategorien

Order via subito

Inter-library loan at ZB MED

Full text online

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

Full text online

More links

Kategorien

In stock of ZB MED Bonn / Germany

Order via subito

Inter-library loan at ZB MED

More links

Kategorien

Order via subito

Full text online

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

More links

Kategorien

Order via subito

Inter-library loan at ZB MED