Article ; Online: MiR-144-3p inhibits the proliferation and metastasis of lung cancer A549 cells via targeting HGF.
Journal of cardiothoracic surgery
2022 Volume 17, Issue 1, Page(s) 117
Abstract: Aim: MicroRNAs have been confirmed as vital regulators in gene expression, which could affect multiple cancer cell biological behaviors. This study aims to elucidate the molecular mechanism of miR-144-3p in lung cancer cellular proliferation and ... ...
| Abstract | Aim: MicroRNAs have been confirmed as vital regulators in gene expression, which could affect multiple cancer cell biological behaviors. This study aims to elucidate the molecular mechanism of miR-144-3p in lung cancer cellular proliferation and metastasis. Methods: MiR-144-3p expression in lung cancer tissues and cell lines was detected by qRT-PCR. HGF was predicted as the target gene of miR-144-3p using TargetScan and dual luciferase reporter assay. Immunohistochemistry and qRT-PCR were used to explore the impacts of HCF on lung cancer tissues and cell lines. Impacts of miR-144-3p and HGF on cancer cellular proliferation, migration and invasion were elucidated by CCK-8, Flow cytometry, Transwell invasion and Wound-healing assay. Moreover, nude mouse xenograft model was established to evaluate the effects of miR-144-3p on lung cancer cells. Results: MiR-144-3p exhibited a reduction in both lung cancer tissues and cell lines. HGF was a direct target of miR-144-3p. In contrast to the miR-144-3p expression level, HGF showed a higher level in lung cancer tissues and cell lines. Overexpression miR-144-3p suppressed A549 and NCI-H1299 cell proliferation and metastasis, whereas this was reversed by HGF. MiR-144-3p exhibited an inhibitory effect on A549 cell-induced tumor growth of nude mice. Conclusions: This study reveals miR-144-3p/HGF axis may be involved in the suppression of lung cancer cellular proliferation and development, and miR-144-3p may function as a potential therapeutic target in lung cancer treatment in the future. |
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| MeSH term(s) | A549 Cells ; Animals ; Cell Line, Tumor ; Cell Movement ; Cell Proliferation/genetics ; Gene Expression Regulation, Neoplastic ; Hepatocyte Growth Factor/genetics ; Hepatocyte Growth Factor/metabolism ; Hepatocyte Growth Factor/pharmacology ; Humans ; Lung Neoplasms/pathology ; Mice ; Mice, Nude ; MicroRNAs/genetics ; MicroRNAs/metabolism |
| Chemical Substances | HGF protein, human ; MIRN144 microRNA, human ; MicroRNAs ; Hepatocyte Growth Factor (67256-21-7) |
| Language | English |
| Publishing date | 2022-05-14 |
| Publishing country | England |
| Document type | Journal Article |
| ZDB-ID | 2227224-0 |
| ISSN | 1749-8090 ; 1749-8090 |
| ISSN (online) | 1749-8090 |
| ISSN | 1749-8090 |
| DOI | 10.1186/s13019-022-01861-3 |
| Database | MEDical Literature Analysis and Retrieval System OnLINE |
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