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  1. Article: Evaluation of a Lateral Flow Immunoassay for Rapid Detection of CTX-M Producers from Blood Cultures.

    Fang, Hanshu / Lee, Chung-Ho / Cao, Huiluo / Jiang, Shuo / So, Simon Yung-Chun / Tse, Cindy Wing-Sze / Cheng, Vincent Chi-Chung / Ho, Pak-Leung

    Microorganisms

    2023  Volume 11, Issue 1

    Abstract: Bacteremia caused by extended-spectrum β-lactamases- ... ...

    Abstract Bacteremia caused by extended-spectrum β-lactamases-producing
    Language English
    Publishing date 2023-01-04
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2720891-6
    ISSN 2076-2607
    ISSN 2076-2607
    DOI 10.3390/microorganisms11010128
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: Antimicrobial susceptibility of Bacteroides fragilis group organisms in Hong Kong, 2020-2021.

    Fang, Hanshu / Li, Xin / Yan, Mei-Kum / Tong, Man-Ki / Chow, Kin-Hung / Cheng, Vincent Chi-Chung / Ho, Pak-Leung

    Anaerobe

    2023  Volume 82, Page(s) 102756

    Abstract: Objectives: This retrospective study analyzed the susceptibility levels of Bacteroides fragilis group (BFG) in a hospital-based laboratory where disk diffusion test (DDT) was routinely performed. Isolates non-susceptible to imipenem and metronidazole by ...

    Abstract Objectives: This retrospective study analyzed the susceptibility levels of Bacteroides fragilis group (BFG) in a hospital-based laboratory where disk diffusion test (DDT) was routinely performed. Isolates non-susceptible to imipenem and metronidazole by DDT were further investigated using a gradient method.
    Methods: The DDT and MIC susceptibility data of clindamycin, metronidazole, moxifloxacin and imipenem obtained on Brucella blood agar for 1264 non-duplicated isolates during 2020-2021 were analyzed. Species identification was obtained by matrix-assisted laser desorption ionization time-of-flight mass spectrometry and 16S rRNA sequencing. Interpretative agreement of DDT results using the 2015 EUCAST tentative and 2021 CA-SFM breakpoints was compared against MIC as the reference.
    Results: The dataset included 604 B. fragilis (483 division I, 121 division II isolates), 415 non-fragilis Bacteroides, 177 Phocaeicola and 68 Parabacteroides. Susceptibility rates for clindamycin (22.1-62.1%) and moxifloxacin (59.9-80.9%) were low and many had no inhibition zones. At the EUCAST and CA-SFM breakpoints, 83.0 and 89.4% were imipenem-susceptible, and 89.6% and 97.4 were metronidazole-susceptible. MIC testing confirmed 11.4% and 2.8% isolates as imipenem-non-susceptible and metronidazole-resistant, respectively. Significant numbers of false-susceptibility and/or false-resistance results were observed at the CA-SFM breakpoint but not the EUCAST breakpoint. Higher rates of imipenem and/or metronidazole resistance were detected in B. fragilis division II, B. caccae, B. ovatus, B. salyersiae, B. stercoris and Parabacteroides. Co-resistance to imipenem and metronidazole was detected in 3 B. fragilis division II isolates.
    Conclusions: The data demonstrated emerging BFG resistance to several important anti-anaerobic antibiotics and highlights the importance of anaerobic susceptibility testing in clinical laboratories to guide therapy.
    MeSH term(s) Bacteroides ; Bacteroides fragilis ; Clindamycin ; Metronidazole ; Moxifloxacin ; Hong Kong ; Retrospective Studies ; RNA, Ribosomal, 16S/genetics ; Microbial Sensitivity Tests ; Anti-Bacterial Agents/pharmacology ; Imipenem/pharmacology
    Chemical Substances Clindamycin (3U02EL437C) ; Metronidazole (140QMO216E) ; Moxifloxacin (U188XYD42P) ; RNA, Ribosomal, 16S ; Anti-Bacterial Agents ; Imipenem (71OTZ9ZE0A)
    Language English
    Publishing date 2023-07-08
    Publishing country England
    Document type Journal Article
    ZDB-ID 1237621-8
    ISSN 1095-8274 ; 1075-9964
    ISSN (online) 1095-8274
    ISSN 1075-9964
    DOI 10.1016/j.anaerobe.2023.102756
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 4318: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (2.OG)
    ab Jg. 2022: Lesesaal (EG)

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  3. Article ; Online: Antimicrobial susceptibility of Bacteroides fragilis group organisms in Hong Kong, 2020–2021

    Fang, Hanshu / Li, Xin / Yan, Mei-Kum / Tong, Man-Ki / Chow, Kin-Hung / Cheng, Vincent Chi-Chung / Ho, Pak Leung

    Anaerobe. 2023 Aug., v. 82 p.102756-

    2023  

    Abstract: This retrospective study analyzed the susceptibility levels of Bacteroides fragilis group (BFG) in a hospital-based laboratory where disk diffusion test (DDT) was routinely performed. Isolates non-susceptible to imipenem and metronidazole by DDT were ... ...

    Abstract This retrospective study analyzed the susceptibility levels of Bacteroides fragilis group (BFG) in a hospital-based laboratory where disk diffusion test (DDT) was routinely performed. Isolates non-susceptible to imipenem and metronidazole by DDT were further investigated by gradient method. The DDT and MIC susceptibility data of clindamycin, metronidazole, moxifloxacin and imipenem obtained on Brucella blood agar for 1264 non-duplicated isolates during 2020–2021 were analyzed. Species identification was obtained by matrix-assisted laser desorption ionization time-of-flight mass spectrometry and 16S rRNA sequencing. Interpretative agreement of DDT results using the 2015 EUCAST tentative and 2021 CA-SFM breakpoints was compared against MIC as the reference. The dataset included 604 B. fragilis (483 division I, 121 division II isolates), 415 non-fragilis Bacteroides, 177 Phocaeicola and 68 Parabacteroides. Susceptibility rates for clindamycin (22.1–62.1%) and moxifloxacin (59.9–80.9%) were low and many had no inhibition zones. At the EUCAST and CA-SFM breakpoints, 83.0 and 89.4% were imipenem-susceptible, and 89.6% and 97.4 were metronidazole-susceptible. MIC testing confirmed 11.4% and 2.8% isolates as imipenem-non-susceptible and metronidazole-resistant, respectively. Significant numbers of false-susceptibility and/or false-resistance results were observed at the CA-SFM breakpoint but not the EUCAST breakpoint. Higher rates of imipenem and/or metronidazole resistance were detected in B. fragilis division II, B. caccae, B. ovatus, B. salyersiae, B. stercoris and Parabacteroides. Co-resistance to imipenem and metronidazole was detected in 3 B. fragilis division II isolates. The data demonstrated emerging BFG resistance to several important anti-anaerobic antibiotics and highlights the importance of anaerobic susceptibility testing in clinical laboratories to guide therapy.
    Keywords Bacteroides fragilis ; Brucella ; antibiotic resistance ; clindamycin ; culture media ; data collection ; disk diffusion antimicrobial test ; imipenem ; metronidazole ; moxifloxacin ; retrospective studies ; species identification ; therapeutics ; China
    Language English
    Dates of publication 2023-08
    Publishing place Elsevier Ltd
    Document type Article ; Online
    ZDB-ID 1237621-8
    ISSN 1075-9964
    ISSN 1075-9964
    DOI 10.1016/j.anaerobe.2023.102756
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    In stock of ZB MED Cologne/Königswinter

    Zs.A 4318: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (2.OG)
    ab Jg. 2022: Lesesaal (EG)

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  4. Article ; Online: The influence of cell morphology on microfluidic single cell analysis.

    Zhang, Xuxin / Li, Yanzhao / Fang, Hanshu / Wei, Hongquan / Mu, Ying / Lang, Ming-Fei / Sun, Jing

    RSC advances

    2018  Volume 9, Issue 1, Page(s) 139–144

    Abstract: Microfluidics has been widely used in single cell analysis. Current protocols allow either spread or round cells to be analyzed. However, the contribution of cell morphology to single cell analysis has not been noted. In this study, four proteins (EGFR, ... ...

    Abstract Microfluidics has been widely used in single cell analysis. Current protocols allow either spread or round cells to be analyzed. However, the contribution of cell morphology to single cell analysis has not been noted. In this study, four proteins (EGFR, PTEN, pAKT, and pS6) in the EGFR signaling pathway are measured simultaneously using microfluidic image cytometry (MIC) in glioblastoma cells U87. The results show that the MIC technology can reveal different subsets of cells corresponding to the four protein expression levels no matter whether they are round or spread at the time of the measurements. However, sharper distinction is obtained from round cells, which implies that cellular heterogeneity can be better resolved with round cells during
    Language English
    Publishing date 2018-12-21
    Publishing country England
    Document type Journal Article
    ISSN 2046-2069
    ISSN (online) 2046-2069
    DOI 10.1039/c8ra08303g
    Database MEDical Literature Analysis and Retrieval System OnLINE

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