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Article ; Online: Long-term monitoring of ultratrace nucleic acids using tetrahedral nanostructure-based NgAgo on wearable microneedles.

Yang, Bin / Wang, Haonan / Kong, Jilie / Fang, Xueen

2024 Volume 15, Issue 1, Page(s) 1936

Abstract: Real-time and continuous monitoring of nucleic acid biomarkers with wearable devices holds potential for personal health management, especially in the context of pandemic surveillance or intensive care unit disease. However, achieving high sensitivity ... ...

Abstract	Real-time and continuous monitoring of nucleic acid biomarkers with wearable devices holds potential for personal health management, especially in the context of pandemic surveillance or intensive care unit disease. However, achieving high sensitivity and long-term stability remains challenging. Here, we report a tetrahedral nanostructure-based Natronobacterium gregoryi Argonaute (NgAgo) for long-term stable monitoring of ultratrace unamplified nucleic acids (cell-free DNAs and RNAs) in vivo for sepsis on wearable device. This integrated wireless wearable consists of a flexible circuit board, a microneedle biosensor, and a stretchable epidermis patch with enrichment capability. We comprehensively investigate the recognition mechanism of nucleic acids by NgAgo/guide DNA and signal transformation within the Debye distance. In vivo experiments demonstrate the suitability for real-time monitoring of cell-free DNA and RNA with a sensitivity of 0.3 fM up to 14 days. These results provide a strategy for highly sensitive molecular recognition in vivo and for on-body detection of nucleic acid.
MeSH term(s)	Nucleic Acids ; Natronobacterium/genetics ; DNA ; Wearable Electronic Devices ; Biosensing Techniques ; Cell-Free Nucleic Acids ; Nanostructures
Chemical Substances	Nucleic Acids ; DNA (9007-49-2) ; Cell-Free Nucleic Acids
Language	English
Publishing date	2024-03-02
Publishing country	England
Document type	Journal Article
ZDB-ID	2553671-0
ISSN	2041-1723 ; 2041-1723
ISSN (online)	2041-1723
ISSN	2041-1723
DOI	10.1038/s41467-024-46215-w
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: Binding Activity of Prokaryotic Argonaute for Background-Suppressed Exponential Isothermal Amplification.

Lin, Qiuyuan / Ye, Xin / Chen, Hongyuan / Fang, Xueen / Chen, Hui / Kong, Jilie

Analytical chemistry

2024 Volume 96, Issue 2, Page(s) 620–623

Abstract: Prokaryotic Argonautes (pAgos) have been recently used in many nucleic acid biosensing applications but have rarely been used for regulating the isothermal amplification system. Herein, we ... ...

Abstract	Prokaryotic Argonautes (pAgos) have been recently used in many nucleic acid biosensing applications but have rarely been used for regulating the isothermal amplification system. Herein, we reported
MeSH term(s)	Humans ; MicroRNAs/analysis ; Nucleic Acid Hybridization ; Nucleic Acid Amplification Techniques/methods
Chemical Substances	MicroRNAs
Language	English
Publishing date	2024-01-03
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	1508-8
ISSN	1520-6882 ; 0003-2700
ISSN (online)	1520-6882
ISSN	0003-2700
DOI	10.1021/acs.analchem.3c05263
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Article ; Online: Real-Time Monitoring and Early Warning of a Cytokine Storm In Vivo Using a Wearable Noninvasive Skin Microneedle Patch.

Xu, Jingxian / Yang, Bin / Kong, Jilie / Zhang, Yongjun / Fang, Xueen

Advanced healthcare materials

2023 Volume 12, Issue 18, Page(s) e2203133

Abstract: A cytokine storm may be the last attack of various diseases, such as sepsis, cancer, and coronavirus disease 2019, that can be life threatening. Real-time monitoring of cytokines in vivo is helpful for assessing the immune status of patients and ... ...

Abstract	A cytokine storm may be the last attack of various diseases, such as sepsis, cancer, and coronavirus disease 2019, that can be life threatening. Real-time monitoring of cytokines in vivo is helpful for assessing the immune status of patients and providing an early warning of a cytokine storm. In this study, a functional carbon nanotube biointerface-based wearable microneedle patches for real-time monitoring of a cytokine storm in vivo via electrochemical analysis are reported. This wearable system has sensitivity with a detection limit of 0.54 pg mL
MeSH term(s)	Humans ; Cytokine Release Syndrome/metabolism ; Needles ; COVID-19 ; Skin/metabolism ; Wearable Electronic Devices
Language	English
Publishing date	2023-03-12
Publishing country	Germany
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2649576-4
ISSN	2192-2659 ; 2192-2640
ISSN (online)	2192-2659
ISSN	2192-2640
DOI	10.1002/adhm.202203133
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Article ; Online: Programmable CRISPR-Cas9 microneedle patch for long-term capture and real-time monitoring of universal cell-free DNA.

Yang, Bin / Kong, Jilie / Fang, Xueen

Nature communications

2022 Volume 13, Issue 1, Page(s) 3999

Abstract: Recent advances in biointerfaces have led to the development of wearable devices that can provide insights into personal health. As wearable modules, microneedles can extract analytes of interest from interstitial fluid in a minimally invasive fashion. ... ...

Abstract	Recent advances in biointerfaces have led to the development of wearable devices that can provide insights into personal health. As wearable modules, microneedles can extract analytes of interest from interstitial fluid in a minimally invasive fashion. However, some microneedles are limited by their ability to perform highly effective extraction and real-time monitoring for macromolecule biomarkers simultaneously. Here we show the synergetic effect of CRISPR-activated graphene biointerfaces, and report an on-line wearable microneedle patch for extraction and in vivo long-term monitoring of universal cell-free DNA. In this study, this wearable system enables real-time monitoring of Epstein-Barr virus, sepsis, and kidney transplantation cell-free DNA, with anti-interference ability of 60% fetal bovine serum, and has satisfactory stable sensitivity for 10 days in vivo. The experimental results of immunodeficient mouse models shows the feasibility and practicability of this proposed method. This wearable patch holds great promise for long-term in vivo monitoring of cell-free DNA and could potentially be used for early disease screening and prognosis.
MeSH term(s)	Animals ; CRISPR-Cas Systems/genetics ; Cell-Free Nucleic Acids/genetics ; Epstein-Barr Virus Infections ; Herpesvirus 4, Human/genetics ; Mice ; Needles
Chemical Substances	Cell-Free Nucleic Acids
Language	English
Publishing date	2022-07-09
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2553671-0
ISSN	2041-1723 ; 2041-1723
ISSN (online)	2041-1723
ISSN	2041-1723
DOI	10.1038/s41467-022-31740-3
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Article ; Online: Innovative Highly Specific Nucleic Acid Isothermal Detection Assay Based on the Polymerization-Coupled Endonuclease Activity of Prokaryotic DNA Polymerase I

Li, Pintao / Lin, Qiuyuan / Xiong, Huiwen / Kong, Jilie / Ye, Xin / Fang, Xueen

Analytical Chemistry. 2023 Oct. 12, v. 95, no. 42 p.15755-15762

2023

Abstract: In this study, we developed an innovative highly specific nucleic acid isothermal detection assay based on prokaryotic DNA polymerase I with exquisitely designed fluorescent probes, achieving high sensitivity and 100% specificity within 30 min. The ... ...

Abstract	In this study, we developed an innovative highly specific nucleic acid isothermal detection assay based on prokaryotic DNA polymerase I with exquisitely designed fluorescent probes, achieving high sensitivity and 100% specificity within 30 min. The fluorescent nucleic acid probe was designed and constructed based on the specific flap cleavage endonuclease activity of prokaryotic DNA polymerase I (including the Bst, Bsu, Bsm, and Klenow DNA polymerases). The flap endonuclease activity depends on the length of the flap DNA and polymerization activity, which greatly reduces the false-positive rate caused by primer dimerization. This robust assay was also validated by the detection of rotavirus with great specificity and sensitivity. It could be a great alternative to qPCR in the field of point-of-care detection of pathogens.
Keywords	DNA ; DNA-directed DNA polymerase ; analytical chemistry ; dimerization ; fluorescence ; point-of-care systems ; polymerization
Language	English
Dates of publication	2023-1012
Size	p. 15755-15762.
Publishing place	American Chemical Society
Document type	Article ; Online
ZDB-ID	1508-8
ISSN	1520-6882 ; 0003-2700
ISSN (online)	1520-6882
ISSN	0003-2700
DOI	10.1021/acs.analchem.3c03261
Database	NAL-Catalogue (AGRICOLA)

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Article ; Online: Simultaneous Rapid Nucleic Acid and Protein Detection in a Lateral Chromatography Chip for COVID-19 Diagnosis.

Lin, Qiuyuan / Zhang, Jin / Liu, Liling / Kong, Jilie / Fang, Xueen

ACS omega

2022 Volume 7, Issue 43, Page(s) 38409–38416

Abstract: In this work, we report a fast, portable, and economical microfluidic platform for the simultaneous detection of nucleic acid and proteins. Using SARS-CoV-2 as a target, this microfluidic chip enabled to simultaneously detect the SARS-CoV-2 RNA (N gene) ... ...

Abstract	In this work, we report a fast, portable, and economical microfluidic platform for the simultaneous detection of nucleic acid and proteins. Using SARS-CoV-2 as a target, this microfluidic chip enabled to simultaneously detect the SARS-CoV-2 RNA (N gene) antigen (or specific IgG antibody) with respective detection limits of 1 copy/μL for nucleic acid, 0.85 ng/mL for antigen, and 5.80 ng/mL for IgG within 30 min with high stability and anti-interference ability. The capability of this system in clinical applications was further evaluated using clinical samples, displaying 100% sensitivity and 100% specificity for COVID-19 diagnosis. These findings demonstrate the potential of this method to be used for the detection and subsequent control of pathogens.
Language	English
Publishing date	2022-10-21
Publishing country	United States
Document type	Journal Article
ISSN	2470-1343
ISSN (online)	2470-1343
DOI	10.1021/acsomega.2c03499
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Article ; Online: Rapid identification and quantitation of single plant seed allergen using paper-based microfluidics.

Sun, Xiaodong / Liu, Yongxin / Niu, Bing / Chen, Qin / Fang, Xueen

PloS one

2022 Volume 17, Issue 12, Page(s) e0266775

Abstract: Nucleic acid amplification is a sensitive and powerful tool for allergen detection. However, it is limited due to the relatively cumbersome methods required to extract nucleic acids from single plant seed allergen (e.g. peanut and soybean). In view of ... ...

Abstract	Nucleic acid amplification is a sensitive and powerful tool for allergen detection. However, it is limited due to the relatively cumbersome methods required to extract nucleic acids from single plant seed allergen (e.g. peanut and soybean). In view of this, an approach of extracting nucleic acid with untreated glass-fiber paper (paper-based microfluidics) was applied for nucleic acid capture and purification from plant seed allergen and commercial products. After cut by hollow cylindrical cutter, a certain size the paper chip it used to absorb DNA. And this paper-based microfluidics with DNA was directly applied for amplification by loop-mediated isothermal amplification (LAMP). To evaluate the adsorption performance of paper chip to DNA, CTAB and SDS method were used as comparisons. From amplification results, the established technique has good specificity, high repeatability (C.V. values are 4.41% and 6.17% for peanut and soybean) and favorable sensitivity (7.39 ng/μL or peanut and 6.6 ng/μL for soybean), and successfully used for commercial products (2 kinds of candy and 2 kinds of cakes containing peanut, and 2 kinds of drinks, candy and 2 kinds of biscuits containing soybean). This speed and flexible detection method makes it suit for applications in point-of-care (POC) detection at different scenario, such as custom house and import port.
MeSH term(s)	Nucleic Acid Amplification Techniques/methods ; Microfluidics ; Arachis/genetics ; Allergens/genetics ; Nucleic Acids ; DNA ; Glycine max/genetics ; Seeds
Chemical Substances	Allergens ; Nucleic Acids ; DNA (9007-49-2)
Language	English
Publishing date	2022-12-12
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2267670-3
ISSN	1932-6203 ; 1932-6203
ISSN (online)	1932-6203
ISSN	1932-6203
DOI	10.1371/journal.pone.0266775
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: Accurate identification of SARS-CoV-2 variant delta using graphene/CRISPR-dCas9 electrochemical biosensor.

Yang, Bin / Zeng, Xiaowei / Zhang, Jin / Kong, Jilie / Fang, Xueen

Talanta

2022 Volume 249, Page(s) 123687

Abstract: Delta (B.1.617.2), a highly infectious variant of SARS-CoV-2, has been sweeping the world, and threatening the safety of human life seriously. It is urgent to develop a highly selective and sensitive assay to accurately identify the SARS-CoV-2 variant ... ...

Abstract	Delta (B.1.617.2), a highly infectious variant of SARS-CoV-2, has been sweeping the world, and threatening the safety of human life seriously. It is urgent to develop a highly selective and sensitive assay to accurately identify the SARS-CoV-2 variant Delta. In this work, we constructed a graphene/CRISPR-dCas9 electrochemical biosensor to accurately identify SARS-CoV-2 variant Delta, where the signal was further amplified by embedded electrochemical probe [Ru(phen)
MeSH term(s)	Biosensing Techniques ; COVID-19/diagnosis ; Clustered Regularly Interspaced Short Palindromic Repeats ; Graphite ; Humans ; Reproducibility of Results ; SARS-CoV-2/genetics
Chemical Substances	Graphite (7782-42-5)
Language	English
Publishing date	2022-06-15
Publishing country	Netherlands
Document type	Journal Article
ZDB-ID	1500969-5
ISSN	1873-3573 ; 0039-9140
ISSN (online)	1873-3573
ISSN	0039-9140
DOI	10.1016/j.talanta.2022.123687
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Article ; Online: Innovative Highly Specific Nucleic Acid Isothermal Detection Assay Based on the Polymerization-Coupled Endonuclease Activity of Prokaryotic DNA Polymerase I.

Li, Pintao / Lin, Qiuyuan / Xiong, Huiwen / Kong, Jilie / Ye, Xin / Fang, Xueen

Analytical chemistry

2023 Volume 95, Issue 42, Page(s) 15755–15762

Abstract	In this study, we developed an innovative highly specific nucleic acid isothermal detection assay based on prokaryotic DNA polymerase I with exquisitely designed fluorescent probes, achieving high sensitivity and 100% specificity within 30 min. The fluorescent nucleic acid probe was designed and constructed based on the specific flap cleavage endonuclease activity of prokaryotic DNA polymerase I (including the
MeSH term(s)	DNA Polymerase I ; Polymerization ; Nucleic Acid Amplification Techniques ; DNA ; Endonucleases ; Sensitivity and Specificity
Chemical Substances	DNA Polymerase I (EC 2.7.7.7) ; DNA (9007-49-2) ; Endonucleases (EC 3.1.-)
Language	English
Publishing date	2023-10-12
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	1508-8
ISSN	1520-6882 ; 0003-2700
ISSN (online)	1520-6882
ISSN	0003-2700
DOI	10.1021/acs.analchem.3c03261
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: Wearable chem-biosensing devices: from basic research to commercial market.

Yang, Bin / Jiang, Xingyu / Fang, Xueen / Kong, Jilie

Lab on a chip

2021 Volume 21, Issue 22, Page(s) 4285–4310

Abstract: Wearable chem-biosensors have been garnering tremendous interest due to the significant potential in tailored healthcare diagnostics and therapeutics. With the development of the medical diagnostics revolution, wearable chem-biosensors as a rapidly ... ...

Abstract	Wearable chem-biosensors have been garnering tremendous interest due to the significant potential in tailored healthcare diagnostics and therapeutics. With the development of the medical diagnostics revolution, wearable chem-biosensors as a rapidly emerging wave allow individuals to perform on-demand detection and obtain the required in-depth information. In contrast to commercial wearables, which tend to be miniaturized for measuring physical activities, the recent progressive wearable chem-biosensing device have mainly focused on non-invasive or minimally invasive monitoring biomarkers at the molecular level. Wearables is a multidisciplinary subject, and chem-biosensing is one of the most significant technologies. In this review, the currently basic academic research of wearable chem-biosensing devices and its commercial transformation were summarized and highlighted. Moreover, some representative wearable products on the market for individual health managements are presented. Strategies for the identification and sensing of biomarkers are discussed to further promote the development of wearable chem-biosensing devices. We also shared the limitations and breakthroughs of the next generation of chemo-biosensor wearables, from home use to clinical diagnosis.
MeSH term(s)	Biomarkers ; Biosensing Techniques ; Delivery of Health Care ; Exercise ; Humans ; Wearable Electronic Devices
Chemical Substances	Biomarkers
Language	English
Publishing date	2021-11-09
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't ; Review
ZDB-ID	2056646-3
ISSN	1473-0189 ; 1473-0197
ISSN (online)	1473-0189
ISSN	1473-0197
DOI	10.1039/d1lc00438g
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