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Article ; Online: Tuberculous pleural effusion: diagnosis & management.

Antonangelo, Leila / Faria, Caroline S / Sales, Roberta K

2019 Volume 13, Issue 8, Page(s) 747–759

Abstract: ... ...

Abstract	Background
MeSH term(s)	Adenosine Deaminase/blood ; Biomarkers/blood ; Disease Management ; Drug Therapy, Combination ; Humans ; Interferon-gamma/blood ; Interleukin-27/blood ; Leprostatic Agents ; Pleural Effusion/diagnosis ; Tuberculosis, Pleural/diagnosis ; Tuberculosis, Pleural/drug therapy ; Tuberculosis, Pleural/epidemiology ; Tuberculosis, Pleural/etiology
Chemical Substances	Biomarkers ; Interleukin-27 ; Leprostatic Agents ; Interferon-gamma (82115-62-6) ; Adenosine Deaminase (EC 3.5.4.4)
Language	English
Publishing date	2019-07-03
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't ; Review
ZDB-ID	2479146-5
ISSN	1747-6356 ; 1747-6348
ISSN (online)	1747-6356
ISSN	1747-6348
DOI	10.1080/17476348.2019.1637737
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Article ; Online: Response to: Necessity of co-operation between pulmonologists and internists in tuberculous pleurisy diagnosis.

Antonangelo, Leila / Faria, Caroline S / Sales, Roberta K

Expert review of respiratory medicine

2019 Volume 13, Issue 11, Page(s) 1039

MeSH term(s)	Humans ; Mycobacterium tuberculosis ; Pleural Effusion ; Pulmonologists ; Tuberculosis, Pleural
Language	English
Publishing date	2019-09-17
Publishing country	England
Document type	Letter ; Research Support, Non-U.S. Gov't ; Comment
ZDB-ID	2479146-5
ISSN	1747-6356 ; 1747-6348
ISSN (online)	1747-6356
ISSN	1747-6348
DOI	10.1080/17476348.2019.1667235
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Article ; Online: Performance of the UroVysion

Rosolen, Débora C B / Faria, Daniel K / Faria, Caroline S / Antonangelo, Leila

Cancer medicine

2018 Volume 7, Issue 5, Page(s) 1967–1977

Abstract: The cytological examination of cavity fluids has limited sensitivity in the diagnosis of malignancy. Aneuploidy, which is commonly observed in neoplastic cells, could potentially be used as an ancillary diagnostic tool. To evaluate the detection of ... ...

Abstract	The cytological examination of cavity fluids has limited sensitivity in the diagnosis of malignancy. Aneuploidy, which is commonly observed in neoplastic cells, could potentially be used as an ancillary diagnostic tool. To evaluate the detection of aneuploid cells in cavitary effusion samples using the fluorescence in situ hybridization (FISH) assay UroVysion
MeSH term(s)	Aged ; Aneuploidy ; Chromosome Aberrations ; Cytogenetic Analysis ; Female ; Humans ; In Situ Hybridization, Fluorescence/methods ; Male ; Middle Aged ; Pleural Effusion, Malignant/diagnosis ; Pleural Effusion, Malignant/genetics ; Sensitivity and Specificity
Language	English
Publishing date	2018-03-25
Publishing country	United States
Document type	Journal Article
ZDB-ID	2659751-2
ISSN	2045-7634 ; 2045-7634
ISSN (online)	2045-7634
ISSN	2045-7634
DOI	10.1002/cam4.1442
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Article: Endoscopic transoral outlet reduction induces enterohormonal changes in patients with weight regain after Roux-en-Y Gastric Bypass.

Brunaldi, Vitor Ottoboni / Farias, Galileu Ferreira / de Moura, Diogo Turiani Hourneaux / Santo, Marco Aurélio / Abu Dayyeh, Barham K. / Faria, Caroline Silverio / Antonangelo, Leila / Waitzberg, Dan Linetzki / de Moura, Eduardo Guimarães Hourneaux

Endoscopy International Open

2024

Abstract: Background and aims: Transoral outlet reduction (TORe) has long been employed in treating weight regain after Roux-en-Y gastric bypass. However, its impact on gut hormones and their relationship with weight loss remains unknown. Patients and Methods: ... ...

Abstract	Background and aims: Transoral outlet reduction (TORe) has long been employed in treating weight regain after Roux-en-Y gastric bypass. However, its impact on gut hormones and their relationship with weight loss remains unknown. Patients and Methods: This is a substudy of a previous randomized clinical trial. Adults with significant weight regain and dilated gastrojejunostomy underwent TORe with Argon Plasma Coagulation (APC) alone or APC plus endoscopic suturing (APC-Suture). Serum levels of ghrelin, GLP-1, and PYY were assessed at fasting, 30, 60, 90, and 120 minutes after a standardized liquid meal. Results were compared according to allocation group, clinical success, and history of cholecystectomy. Results: Thirty-six patients (19 APC vs. 17 APC-Suture) were enrolled. There were no significant baseline differences between groups. In all analyses, the typical postprandial decrease in ghrelin levels was delayed by 30 minutes, but no other changes were noted. GLP-1 levels significantly decreased at 12 months in both allocation groups. Similar findings were noted after dividing groups according to the history of cholecystectomy and clinical success. The APC cohort presented an increase in PYY levels at 90 minutes, while APC-Suture did not. Naïve patients had significantly lower PYY levels at baseline (p=0.01) compared to cholecystectomized individuals. This latter group experienced a significant increase in the AUC for PYY levels, while naïve patients did not, leading to a higher AUC at 12 months (p=0.0001). Conclusions: TORe interferes with the dynamics of gut hormones. APC triggers a more pronounced enteroendocrine response than APC-Suture, especially in cholecystectomized patients.
Language	English
Publishing date	2024-04-23
Publisher	Georg Thieme Verlag KG
Publishing place	Stuttgart ; New York
Document type	Article
ZDB-ID	2761052-4
ISSN	2196-9736 ; 2364-3722 ; 2196-9736
ISSN (online)	2196-9736
ISSN	2364-3722 ; 2196-9736
DOI	10.1055/a-2312-5742
Database	Thieme publisher's database

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Article ; Online: How a discerning cytological examination can aid in the diagnosis of infectious diseases: case reports.

Faria, D K / Almeida Júnior, J N de / Faria, C S / Durante, B / Falasco, B F / Terreri Neto, E / Antonangelo, L

Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologicas

2021 Volume 54, Issue 2, Page(s) e10462

Abstract: Infections caused by uncommon and resistant pathogens in unusual sites have been increasingly reported in medical literature. We describe four cases of rare cytological findings and clinical impact for patients. In the first case, Aspergillus sp and ... ...

Abstract	Infections caused by uncommon and resistant pathogens in unusual sites have been increasingly reported in medical literature. We describe four cases of rare cytological findings and clinical impact for patients. In the first case, Aspergillus sp and Pneumocystis jirovecii were observed in the bronchoalveolar lavage of a patient with severe systemic lupus. In the second and third cases, we describe the presence of Trichomonas sp and Strongyloides sp larvae in samples of pleural and peritoneal fluid, respectively. The fourth report is about a patient with a wrist subcutaneous nodule whose synovial aspiration and cytology revealed the presence of brown septate hyphae. The early identification of the infectious agent in the cytological examination was essential for the introduction and/or re-adaptation of therapy in the four cases described. Patients in this report were immunocompromised with severe comorbidities, conditions often associated with unfavorable clinical outcomes.
MeSH term(s)	Animals ; Ascitic Fluid/parasitology ; Aspergillus/isolation & purification ; Bronchoalveolar Lavage Fluid/microbiology ; Communicable Diseases/diagnosis ; Cytodiagnosis/methods ; Fatal Outcome ; Female ; Humans ; Male ; Middle Aged ; Pleural Effusion/parasitology ; Pneumocystis carinii/isolation & purification ; Strongyloides/isolation & purification ; Strongyloidiasis/diagnosis ; Trichomonas/isolation & purification ; Trichomonas Infections/diagnosis
Language	English
Publishing date	2021-01-08
Publishing country	Brazil
Document type	Case Reports ; Journal Article
ZDB-ID	786234-9
ISSN	1414-431X ; 0100-879X
ISSN (online)	1414-431X
ISSN	0100-879X
DOI	10.1590/1414-431X202010462
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Article ; Online: Related Pentacyclic Triterpenes Have Immunomodulatory Activity in Chronic Experimental Visceral Leishmaniasis.

de Jesus, Jéssica Adriana / Laurenti, Márcia Dalastra / Antonangelo, Leila / Faria, Caroline Silvério / Lago, João Henrique Ghilardi / Passero, Luiz Felipe Domingues

Journal of immunology research

2021 Volume 2021, Page(s) 6671287

Abstract: Leishmaniasis is a neglected tropical disease caused by the flagellated protozoa of the ... ...

Abstract	Leishmaniasis is a neglected tropical disease caused by the flagellated protozoa of the genus
MeSH term(s)	Animals ; Antiprotozoal Agents/chemistry ; Antiprotozoal Agents/pharmacology ; Host-Pathogen Interactions/drug effects ; Host-Pathogen Interactions/immunology ; Hydrogen Peroxide/chemistry ; Hydrogen Peroxide/pharmacology ; Immunomodulation/drug effects ; Leishmania donovani/drug effects ; Leishmaniasis, Visceral/drug therapy ; Leishmaniasis, Visceral/immunology ; Leishmaniasis, Visceral/metabolism ; Leishmaniasis, Visceral/parasitology ; Membrane Potential, Mitochondrial/drug effects ; Mice ; Mitochondria/drug effects ; Mitochondria/immunology ; Mitochondria/metabolism ; Molecular Structure ; Pentacyclic Triterpenes/chemistry ; Pentacyclic Triterpenes/pharmacology
Chemical Substances	Antiprotozoal Agents ; Pentacyclic Triterpenes ; Hydrogen Peroxide (BBX060AN9V)
Language	English
Publishing date	2021-02-17
Publishing country	Egypt
Document type	Journal Article
ZDB-ID	2817541-4
ISSN	2314-7156 ; 2314-8861
ISSN (online)	2314-7156
ISSN	2314-8861
DOI	10.1155/2021/6671287
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Article ; Online: Hybrid panel of biomarkers can be useful in the diagnosis of pleural and peritoneal effusions.

Faria, Daniel K / Faria, Caroline S / Doi, Dimitria / Acencio, Milena M P / Antonangelo, Leila

Clinica chimica acta; international journal of clinical chemistry

2019 Volume 497, Page(s) 48–53

Abstract: Background: In clinical practice, pleural and peritoneal effusions are usual diagnosis. We evaluated the performance of a hybrid panel of biomarkers in the diagnosis of the main diseases affecting pleura and/or peritoneum.: Methods: Samples of ... ...

Abstract	Background: In clinical practice, pleural and peritoneal effusions are usual diagnosis. We evaluated the performance of a hybrid panel of biomarkers in the diagnosis of the main diseases affecting pleura and/or peritoneum. Methods: Samples of pleural/ peritoneal fluid from 120 patients were evaluated for: CEA (carcinoembryonic antigen), VEGF-A (vascular endothelial growth factor A), PD-L1/B7-H1 (programmed death-ligand 1), NGAL (neutrophil gelatinase-associated lipocalin), TREM-1 (triggering receptor expressed in myeloid cells type-1) and IFNγ (gamma-interferon) by Luminex®; CALP (Calprotectin) by ELISA, and ADA (adenosine deaminase) by enzymatic deamination. Results: For malignant effusion (ME) diagnosis, CEA and NGAL presented superior performance than VEGF-A, PD-L1 and CALP. A CEA-NGAL association showed good sensitivity (86.6%) and accuracy (79.2%). For non-tuberculous infectious effusion (NTBIE), NGAL presented the best performance with sensitivity (75.0%), specificity (62.0%) and accuracy (65.0%) higher than TREM-1 and CALP; however, when associated, although with good sensitivity, there was important decrease in specificity. For tuberculous pleural effusion (TPE), IFNy-ADA presented excellent sensitivity (100%), specificity (87.6%), NPV (100%) and accuracies (~90%). Conclusions: CEA, NGAL, ADA and IFNy were useful in discriminating ME and TPE. However, for NTBIE diagnosis, the hybrid panel did not demonstrate advantages over the classic parameters.
MeSH term(s)	Adenosine Deaminase/analysis ; Adenosine Deaminase/metabolism ; Adolescent ; Adult ; Aged ; Aged, 80 and over ; Biomarkers/analysis ; Enzyme-Linked Immunosorbent Assay ; Female ; Humans ; Interferon-gamma/analysis ; Interferon-gamma/metabolism ; Male ; Middle Aged ; Paracentesis ; Pleural Effusion, Malignant/diagnosis ; Young Adult
Chemical Substances	Biomarkers ; Interferon-gamma (82115-62-6) ; Adenosine Deaminase (EC 3.5.4.4)
Language	English
Publishing date	2019-07-13
Publishing country	Netherlands
Document type	Journal Article
ZDB-ID	80228-1
ISSN	1873-3492 ; 0009-8981
ISSN (online)	1873-3492
ISSN	0009-8981
DOI	10.1016/j.cca.2019.07.015
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Article ; Online: Blood leukocyte transcriptional modules and differentially expressed genes associated with disease severity and age in COVID-19 patients.

Bando, Silvia Y / Bertonha, Fernanda B / Vieira, Sandra E / de Oliveira, Danielle B L / Chalup, Vanessa N / Durigon, Edison L / Palmeira, Patricia / Curi, Ana Cristina P / Faria, Caroline S / Antonangelo, Leila / Lauterbach, Gerhard da P / Regalio, Fabiane A / Cesar, Roberto M / Moreira-Filho, Carlos A

Scientific reports

2023 Volume 13, Issue 1, Page(s) 898

Abstract: Since the molecular mechanisms determining COVID-19 severity are not yet well understood, there is a demand for biomarkers derived from comparative transcriptome analyses of mild and severe cases, combined with patients' clinico-demographic and ... ...

Abstract	Since the molecular mechanisms determining COVID-19 severity are not yet well understood, there is a demand for biomarkers derived from comparative transcriptome analyses of mild and severe cases, combined with patients' clinico-demographic and laboratory data. Here the transcriptomic response of human leukocytes to SARS-CoV-2 infection was investigated by focusing on the differences between mild and severe cases and between age subgroups (younger and older adults). Three transcriptional modules correlated with these traits were functionally characterized, as well as 23 differentially expressed genes (DEGs) associated to disease severity. One module, correlated with severe cases and older patients, had an overrepresentation of genes involved in innate immune response and in neutrophil activation, whereas two other modules, correlated with disease severity and younger patients, harbored genes involved in the innate immune response to viral infections, and in the regulation of this response. This transcriptomic mechanism could be related to the better outcome observed in younger COVID-19 patients. The DEGs, all hyper-expressed in the group of severe cases, were mostly involved in neutrophil activation and in the p53 pathway, therefore related to inflammation and lymphopenia. These biomarkers may be useful for getting a better stratification of risk factors in COVID-19.
MeSH term(s)	Humans ; Biomarkers/metabolism ; COVID-19/genetics ; Leukocytes/metabolism ; Patient Acuity ; SARS-CoV-2/metabolism ; Transcriptome ; Age Factors
Chemical Substances	Biomarkers
Language	English
Publishing date	2023-01-17
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2615211-3
ISSN	2045-2322 ; 2045-2322
ISSN (online)	2045-2322
ISSN	2045-2322
DOI	10.1038/s41598-023-28227-6
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Article ; Online: Bronchial eosinophils, neutrophils, and CD8 + T cells influence asthma control and lung function in schoolchildren and adolescents with severe treatment-resistant asthma.

Eller, Miriam Cardoso Neves / Pierantozzi Vergani, Karina / Saraiva-Romanholo, Beatriz Mangueira / de Souza Xavier Costa, Natália / de Brito, Jôse Mára / Antonangelo, Leila / Faria, Caroline Silvério / Rodrigues, Joaquim Carlos / Mauad, Thais

Respiratory research

2022 Volume 23, Issue 1, Page(s) 335

Abstract: Background: Studies in adult severe treatment-resistant asthma (STRA) have demonstrated heterogeneous pathophysiology. Studies in the pediatric age group are still scarce, and few include bronchial tissue analysis.: Objective: We investigated 6-18- ... ...

Abstract	Background: Studies in adult severe treatment-resistant asthma (STRA) have demonstrated heterogeneous pathophysiology. Studies in the pediatric age group are still scarce, and few include bronchial tissue analysis. Objective: We investigated 6-18-year-old patients diagnosed with STRA in Sao Paulo, Brazil, by characterizing the different lung compartments and their correlations with asthma control and lung function. Methods: Inflammatory profiles of 13 patients with a confirmed diagnosis of STRA were analyzed using blood, induced sputum, bronchoalveolar lavage, viral and bacterial screens and endobronchial biopsy. Inflammatory cells, cytokines, and basement membrane thickening were tested for correlations with the asthma control test (ACT) and spirometry and plethysmography parameters. Results: Endobronchial biopsy specimens from 11 patients were viable for analysis. All biopsies showed eosinophilic infiltration. Submucosal (SM) eosinophils and neutrophils were correlated with worse lung function (pre-BD FEV1), and SM neutrophils were correlated with fixed obstruction (post-BD FEV1). Intraepithelial (IE) neutrophils were positively correlated with lung function (pre-BD sGaw). CD8 + T cells had the highest density in the IE and SM layers and were positively correlated with ACT and negatively correlated with the cytokines IL1β, IL2, IL5, IL7, IL10, IL12, IL17, GCSF, MCP-1, INF-δ, and TNFα in sputum supernatant. The ASM chymase + mast cell density correlated positively with quality-of-life score (pAQLQ) and ACT. Conclusion: Eosinophils and SM neutrophils correlated with worse lung function, while IE neutrophils correlated with better lung function. Most importantly, CD8 + T cells were abundant in bronchial biopsies of STRA patients and showed protective associations, as did chymase + mast cells.
Language	English
Publishing date	2022-12-09
Publishing country	England
Document type	Journal Article
ZDB-ID	2041675-1
ISSN	1465-993X ; 1465-993X
ISSN (online)	1465-993X
ISSN	1465-993X
DOI	10.1186/s12931-022-02259-4
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Article: Transient abnormal myelopoiesis with pericardial effusion in Down syndrome: Case report.

Falasco, Bianca Francisco / Durante, Brenda / Faria, Daniel Kanaan / Faria, Caroline Silvério / Rosolen, Débora Cristina Batista / Antonangelo, Leila

Clinical case reports

2019 Volume 7, Issue 7, Page(s) 1280–1284

Abstract: Pericardial effusion associated with transient abnormal myelopoiesis in Down's syndrome neonates needs to be diagnosed in a timely manner, and the comorbidities must be treated to prevent mortality. To our knowledge, the occurrence of basophilic/ ... ...

Abstract	Pericardial effusion associated with transient abnormal myelopoiesis in Down's syndrome neonates needs to be diagnosed in a timely manner, and the comorbidities must be treated to prevent mortality. To our knowledge, the occurrence of basophilic/eosinophilic pericardial effusion without an increase of these cells in the peripheral blood and with no evidence of associated hypothyroidism is rare.
Language	English
Publishing date	2019-05-17
Publishing country	England
Document type	Case Reports
ZDB-ID	2740234-4
ISSN	2050-0904
ISSN	2050-0904
DOI	10.1002/ccr3.2184
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