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Article ; Online: Potential Way to Develop Dengue Virus Detection in

Rachmawati, Yenny / Ekawardhani, Savira / Fauziah, Nisa / Faridah, Lia / Watanabe, Kozo

Tropical medicine and infectious disease

2024 Volume 9, Issue 3

Abstract: The burden of dengue has emerged as a serious public health issue due to its impact on morbidity, mortality, and economic burden. Existing surveillance systems are inadequate to provide the necessary data for the prompt and efficient control of dengue. ... ...

Abstract	The burden of dengue has emerged as a serious public health issue due to its impact on morbidity, mortality, and economic burden. Existing surveillance systems are inadequate to provide the necessary data for the prompt and efficient control of dengue. Passive surveillance of dengue cases may lead to underreporting and delayed mitigation responses. Improved dengue control program requires sensitive and proactive methods for early detection of dengue. We collected and reviewed existing research articles worldwide on detecting dengue virus in
Language	English
Publishing date	2024-03-11
Publishing country	Switzerland
Document type	Journal Article ; Review
ISSN	2414-6366
ISSN (online)	2414-6366
DOI	10.3390/tropicalmed9030060
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Article ; Online: The Use of Biomaterials in Three-Dimensional Culturing of Cancer Cells.

Hanasti, Novia / Faridah, Lia / Fibriani, Azzania / Wiraswati, Hesti Lina / Kusumawaty, Diah / Ekawardhani, Savira

Current issues in molecular biology

2023 Volume 45, Issue 2, Page(s) 1100–1112

Abstract: Cell culture is an important tool in biological research. Most studies use 2D cell culture, but cells grown in 2D cell culture have drawbacks, including limited cell and cell-extracellular matrix interactions, which make it inaccurate to model conditions ...

Abstract	Cell culture is an important tool in biological research. Most studies use 2D cell culture, but cells grown in 2D cell culture have drawbacks, including limited cell and cell-extracellular matrix interactions, which make it inaccurate to model conditions in vivo. Anticancer drug screening is an important research and development process for developing new drugs. As an experiment to mimic the cancer environment in vivo, several studies have been carried out on 3-dimensional (3D) cell cultures with added biomaterials. The use of hydrogel in 3D culture cells is currently developing. The type of hydrogel used might influence cell morphology, viability, and drug screening outcome. Therefore, this review discusses 3D cell culture research regarding the addition of biomaterials.
Language	English
Publishing date	2023-01-30
Publishing country	Switzerland
Document type	Journal Article ; Review
ZDB-ID	2000024-8
ISSN	1467-3045 ; 1467-3037
ISSN (online)	1467-3045
ISSN	1467-3037
DOI	10.3390/cimb45020073
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Article ; Online: Optimal Validated Multi-Factorial Climate Change Risk Assessment for Adaptation Planning and Evaluation of Infectious Disease: A Case Study of Dengue Hemorrhagic Fever in Indonesia.

Faridah, Lia / Suroso, Djoko Santoso Abi / Fitriyanto, Muhammad Suhardjono / Andari, Clarisa Dity / Fauzi, Isnan / Kurniawan, Yonatan / Watanabe, Kozo

Tropical medicine and infectious disease

2022 Volume 7, Issue 8

Abstract: 1) Background: This paper will present an elaboration of the risk assessment methodology by Deutsche Gesellschaft für Internationale Zusammenarbeit GmbH (GIZ), Eurac Research and United Nations University Institute for Environment and Human Security ( ... ...

Abstract	(1) Background: This paper will present an elaboration of the risk assessment methodology by Deutsche Gesellschaft für Internationale Zusammenarbeit GmbH (GIZ), Eurac Research and United Nations University Institute for Environment and Human Security (UNU-EHS) for the assessment of dengue. (2) Methods: We validate the risk assessment model by best-fitting it with the number of dengue cases per province using the least-square fitting method. Seven out of thirty-four provinces in Indonesia were chosen (North Sumatra, Jakarta Capital, West Java, Central Java, East Java, Bali and East Kalimantan). (3) Results: A risk assessment based on the number of dengue cases showed an increased risk in 2010, 2015 and 2016 in which the effects of El Nino and La Nina extreme climates occurred. North Sumatra, Bali, and West Java were more influenced by the vulnerability component, in line with their risk analysis that tends to be lower than the other provinces in 2010, 2015 and 2016 when El Nino and La Nina occurred. (4) Conclusion: Based on data from the last ten years, in Jakarta Capital, Central Java, East Java and East Kalimantan, dengue risks were mainly influenced by the climatic hazard component while North Sumatra, Bali and West Java were more influenced by the vulnerability component.
Language	English
Publishing date	2022-08-08
Publishing country	Switzerland
Document type	Journal Article
ISSN	2414-6366
ISSN (online)	2414-6366
DOI	10.3390/tropicalmed7080172
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Article ; Online: Evaluation of Health Information System (HIS) in The Surveillance of Dengue in Indonesia: Lessons from Case in Bandung, West Java.

Faridah, Lia / Rinawan, Fedri Ruluwedrata / Fauziah, Nisa / Mayasari, Wulan / Dwiartama, Angga / Watanabe, Kozo

International journal of environmental research and public health

2020 Volume 17, Issue 5

Abstract: This study was performed to evaluate the health information system regarding the dengue surveillance system in Indonesia. Major obstacles to the implementation of an effective health information system regarding dengue cases in Bandung are examined, and ... ...

Abstract	This study was performed to evaluate the health information system regarding the dengue surveillance system in Indonesia. Major obstacles to the implementation of an effective health information system regarding dengue cases in Bandung are examined, and practical suggestions on measures to overcome them are discussed. The study utilized a mixed-method research design using qualitative approaches: document analysis, key informants and focus group interviews. Thirty key informants were selected, comprised of policymakers, senior managers, and staff at the Ministry of Health. Data from documents and transcripts were evaluated through a modified Institutional Analysis and Development (IAD) framework described by Ostrom. Through this study, we have identified several issues that hinder the effective implementation of the health information system in the case of dengue in Bandung. In the end, we propose several recommendations for reform that encompasses motivational, strategic, and structural approaches to each component of the analysis. Through evaluation of the health information system for dengue surveillance in Indonesia, we conclude that well-coordination in multi-level governance in a country as large as Indonesia is the key in the implementation of the health information system in different levels of agencies. Furthermore, the adaptability of human resources in adopting a new information system also plays an important part.
MeSH term(s)	Dengue ; Government Agencies ; Health Information Systems/standards ; Humans ; Indonesia ; Public Health Surveillance/methods
Language	English
Publishing date	2020-03-10
Publishing country	Switzerland
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ISSN	1660-4601
ISSN (online)	1660-4601
DOI	10.3390/ijerph17051795
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Article ; Online: Optimization of nucleic acid extraction methods for rapid detection in pandemic situations or diseases with high prevalence.

Wiraswati, Hesti Lina / Ma'ruf, Ilma Fauziah / Ekawardhani, Savira / Faridah, Lia / Laelalugina, Amila / Septanto, Harry / Djati, Imam Damar / Gaffar, Shabarni / Awaludin, Asif

Journal of pharmaceutical analysis

2023 Volume 13, Issue 12, Page(s) 1577–1579

Abstract: Image 1. ...

Abstract	Image 1.
Language	English
Publishing date	2023-08-12
Publishing country	China
Document type	Journal Article
ZDB-ID	2630174-X
ISSN	2214-0883 ; 2095-1779
ISSN (online)	2214-0883
ISSN	2095-1779
DOI	10.1016/j.jpha.2023.08.005
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Article: Spatial and temporal analysis of hospitalized dengue patients in Bandung: demographics and risk.

Faridah, Lia / Mindra, I Gede Nyoman / Putra, Ramadhani Eka / Fauziah, Nisa / Agustian, Dwi / Natalia, Yessika Adelwin / Watanabe, Kozo

Tropical medicine and health

2021 Volume 49, Issue 1, Page(s) 44

Abstract: Background: Bandung, the fourth largest city in Indonesia and capital of West Java province, has been considered a major endemic area of dengue, and studies show that the incidence in this city could increase and spread rapidly. At the same time, ... ...

Abstract	Background: Bandung, the fourth largest city in Indonesia and capital of West Java province, has been considered a major endemic area of dengue, and studies show that the incidence in this city could increase and spread rapidly. At the same time, estimation of incidence could be inaccurate due to a lack of reliable surveillance systems. To provide strategic information for the dengue control program in the face of limited capacity, this study used spatial pattern analysis of a possible outbreak of dengue cases, through the Geographic Information System (GIS). To further enhance the information needed for effective policymaking, we also analyzed the demographic pattern of dengue cases. Methods: Monthly reports of dengue cases from January 2014 to December 2016 from 16 hospitals in Bandung were collected as the database, which consisted of address, sex, age, and code to anonymize the patients. The address was then transformed into geocoding and used to estimate the relative risk of a particular area's developing a cluster of dengue cases. We used the kernel density estimation method to analyze the dynamics of change of dengue cases. Results: The model showed that the spatial cluster of the relative risk of dengue incidence was relatively unchanged for 3 years. Dengue high-risk areas predominated in the southern and southeastern parts of Bandung, while low-risk areas were found mostly in its western and northeastern regions. The kernel density estimation showed strong cluster groups of dengue cases in the city. Conclusions: This study demonstrated a strong pattern of reported cases related to specific demographic groups (males and children). Furthermore, spatial analysis using GIS also visualized the dynamic development of the aggregation of disease incidence (hotspots) for dengue cases in Bandung. These data may provide strategic information for the planning and design of dengue control programs.
Language	English
Publishing date	2021-05-26
Publishing country	Japan
Document type	Journal Article
ZDB-ID	2209835-5
ISSN	1349-4147 ; 1348-8945
ISSN (online)	1349-4147
ISSN	1348-8945
DOI	10.1186/s41182-021-00329-9
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Article ; Online: Evaluation and Clinical Validation of Guanidine-Based Inactivation Transport Medium for Preservation of SARS-CoV-2.

Wiraswati, Hesti L / Gaffar, Shabarni / Ekawardhani, Savira / Fauziah, Nisa / Rinawan, Fedri R / Widyatmoko, Leonardus / Laelalugina, Amila / Arimdayu, Annissa R / Kusniati, Tri / Andari, Clarisa D / Faridah, Lia

Advances in pharmacological and pharmaceutical sciences

2022 Volume 2022, Page(s) 1677621

Abstract: WHO declared the outbreak of COVID-19, caused by SARS-CoV-2, a pandemic in March 2020. More than 223 million cases and approximately 4.6 million deaths have been confirmed. Early diagnosis and immediate treatment became a priority during this pandemic. ... ...

Abstract	WHO declared the outbreak of COVID-19, caused by SARS-CoV-2, a pandemic in March 2020. More than 223 million cases and approximately 4.6 million deaths have been confirmed. Early diagnosis and immediate treatment became a priority during this pandemic. However, COVID-19 diagnostic testing resources are limited, especially early in the pandemic. Apart from being limited, the COVID-19 diagnostic tests using reverse transcription polymerase chain reaction (RT-PCR) have encountered storage, transportation, and safety issues. These problems are mainly experienced by developing poor countries, countries in the equatorial region, and archipelagic countries. VITPAD® is a guanidine-based inactivation transport medium (ITM) formulated to maintain the RNA quality of SARS-CoV-2 during transportation without cold chains. This study, conducted from September 2020 to March 2021, performed clinical validation of VITPAD® by comparing its performance with a globally commercially available ITM from the NEST brand. Its stability at room temperature, safety, and resistance at high temperatures was also tested using RT-PCR analysis. VITPAD® can reduce the infectious nature of the specimen, preserve the SARS-CoV-2 for 18 days at an ambient temperature, and resist high temperatures (40°C for 3 hours). A guanidine-based transport medium, such as VITPAD®, is compatible and recommended for RT-PCR-based molecular diagnosis of COVID-19.
Language	English
Publishing date	2022-07-21
Publishing country	England
Document type	Journal Article
ZDB-ID	3026687-7
ISSN	2633-4690 ; 2633-4682 ; 2633-4690
ISSN (online)	2633-4690 ; 2633-4682
ISSN	2633-4690
DOI	10.1155/2022/1677621
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Article ; Online: Temporal Correlation Between Urban Microclimate, Vector Mosquito Abundance, and Dengue Cases.

Faridah, Lia / Fauziah, Nisa / Agustian, Dwi / Mindra Jaya, I Gede Nyoman / Eka Putra, Ramadhani / Ekawardhani, Savira / Hidayath, Nurrachman / Damar Djati, Imam / Carvajal, Thaddeus M / Mayasari, Wulan / Ruluwedrata Rinawan, Fedri / Watanabe, Kozo

Journal of medical entomology

2022 Volume 59, Issue 3, Page(s) 1008–1018

Abstract: Dengue Hemorrhagic Fever (DHF) is a major mosquito-borne viral disease. Studies have reported a strong correlation between weather, the abundance of Aedes aegypti, the vector of DHF virus, and dengue incidence. However, this conclusion has been based on ... ...

Abstract	Dengue Hemorrhagic Fever (DHF) is a major mosquito-borne viral disease. Studies have reported a strong correlation between weather, the abundance of Aedes aegypti, the vector of DHF virus, and dengue incidence. However, this conclusion has been based on the general climate pattern of wide regions. In general, however, the human population, level of infrastructure, and land-use change in rural and urban areas often produce localized climate patterns that may influence the interaction between climate, vector abundance, and dengue incidence. Thoroughly understanding this correlation will allow the development of a customized and precise local early warning system. To achieve this purpose, we conducted a cohort study, during January-December 2017, in 16 districts in Bandung, West Java, Indonesia. In the selected areas, local weather stations and modified light mosquito traps were set up to obtain data regarding daily weather and the abundance of adult female Ae. aegypti. A generalized linear model was applied to analyze the effect of local weather and female adult Ae. aegypti on the number of dengue cases. The result showed a significant non-linear correlation among mosquito abundance, maximum temperature, and dengue cases. Using our model, the data showed that the addition of a single adult Ae. aegypti mosquito increased the risk of dengue infection by 1.8%, while increasing the maximum temperature by one degree decreased the risk by 17%. This finding suggests specific actionable insights needed to supplement existing mosquito eradication programs.
MeSH term(s)	Aedes ; Animals ; Cohort Studies ; Dengue ; Female ; Humans ; Insect Vectors ; Microclimate ; Mosquito Vectors
Language	English
Publishing date	2022-03-19
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	410635-0
ISSN	1938-2928 ; 0022-2585
ISSN (online)	1938-2928
ISSN	0022-2585
DOI	10.1093/jme/tjac005
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Article: Temporal Correlation Between Urban Microclimate, Vector Mosquito Abundance, and Dengue Cases

Faridah, Lia / Fauziah, Nisa / Agustian, Dwi / Nyoman Mindra Jaya, I Gede / Putra, Ramadhani Eka / Ekawardhani, Savira / Hidayath, Nurrachman / Djati, Imam Damar / Carvajal, Thaddeus M. / Mayasari, Wulan / Rinawan, Fedri Ruluwedrata / Watanabe, Kozo

Journal of medical entomology. 2022 Mar. 19, v. 59, no. 3

2022

Abstract	Dengue Hemorrhagic Fever (DHF) is a major mosquito-borne viral disease. Studies have reported a strong correlation between weather, the abundance of Aedes aegypti, the vector of DHF virus, and dengue incidence. However, this conclusion has been based on the general climate pattern of wide regions. In general, however, the human population, level of infrastructure, and land-use change in rural and urban areas often produce localized climate patterns that may influence the interaction between climate, vector abundance, and dengue incidence. Thoroughly understanding this correlation will allow the development of a customized and precise local early warning system. To achieve this purpose, we conducted a cohort study, during January-December 2017, in 16 districts in Bandung, West Java, Indonesia. In the selected areas, local weather stations and modified light mosquito traps were set up to obtain data regarding daily weather and the abundance of adult female Ae. aegypti. A generalized linear model was applied to analyze the effect of local weather and female adult Ae. aegypti on the number of dengue cases. The result showed a significant non-linear correlation among mosquito abundance, maximum temperature, and dengue cases. Using our model, the data showed that the addition of a single adult Ae. aegypti mosquito increased the risk of dengue infection by 1.8%, while increasing the maximum temperature by one degree decreased the risk by 17%. This finding suggests specific actionable insights needed to supplement existing mosquito eradication programs.
Keywords	Aedes aegypti ; adults ; cohort studies ; dengue hemorrhagic fever ; females ; human population ; infrastructure ; land use change ; linear models ; medical entomology ; microclimate ; risk ; temperature ; viruses ; weather ; Indonesia
Language	English
Dates of publication	2022-0319
Size	p. 1008-1018.
Publishing place	Entomological Society of America
Document type	Article
ZDB-ID	410635-0
ISSN	0022-2585
ISSN	0022-2585
DOI	10.1093/jme/tjac005
Database	NAL-Catalogue (AGRICOLA)

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Article ; Online: The performance of point-of-care antibody test for COVID-19 diagnosis in a tertiary hospital in Bandung, Indonesia.

Fauziah, Nisa / Koesoemadinata, Raspati C / Andriyoko, Basti / Faridah, Lia / Riswari, Silvita F / Widyatmoko, Leonardus / Prihatni, Delita / Ekawardhani, Savira / Fibriani, Azzania / Rachmawati, Emma / Ristandi, Ryan B / Alamanda, Cut Nc / Prodjosoewojo, Susantina / Lestari, Bony W / Santoso, Ida P

Journal of infection in developing countries

2021 Volume 15, Issue 2, Page(s) 237–241

Abstract: Introduction: We aim to describe the performance of combined IgM and IgG point-of-care antibody test (POC-Ab) (Wondfo®) compared to real-time reverse transcriptase (rRT-PCR) (Allplex™ 2019-nCoV Assay) in detecting coronavirus disease 2019 (COVID-19).: ...

Abstract	Introduction: We aim to describe the performance of combined IgM and IgG point-of-care antibody test (POC-Ab) (Wondfo®) compared to real-time reverse transcriptase (rRT-PCR) (Allplex™ 2019-nCoV Assay) in detecting coronavirus disease 2019 (COVID-19). Methodology: We compared POC-Ab with rRT-PCR results among patients in a tertiary hospital from January to March 2020 in Bandung, Indonesia. We selected presumptive COVID-19 patients with positive rRT-PCR consecutively and 20 patients with negative rRT-PCR results were selected randomly from the same group of patients as controls. We described the sensitivity, specificity, positive predictive value (PPV), negative predictive value (NPV) with corresponding 95% confidence interval using serum and capillary blood samples. We also tested POC-Ab using non-COVID-19 (confirmed dengue and typhoid) patients' sera. Results: Twenty-seven patients with positive rRT-PCR result and 20 negative controls were included (68.1% males, mean age 46 (SD: 15.4)). Using the serum, the sensitivity of the POC-Ab was 63.0% (42.4-80.6), specificity was 95.0% (75.1-99.9), PPV was 94.4% (72.7-99.8), NPV was 65.5% (45.7-82.1). A subset of 20 patients was tested using a capillary blood sample. The accuracy of the capillary blood sample is lower compared to serum (50.0% vs. 78.7%). None of the non-COVID-19 sera tested were reactive. Conclusions: POC-Ab for COVID-19 has a high specificity with no false-positive result in non-COVID-19 sera. Therefore, it can be used to guide diagnostic among symptomatic patients in resource limited settings. Given its low sensitivity, patients with high suspicion of COVID-19 but non-reactive result should be prioritized for rRT-PCR testing.
MeSH term(s)	Adult ; Aged ; COVID-19/diagnosis ; COVID-19/etiology ; COVID-19 Nucleic Acid Testing/methods ; COVID-19 Serological Testing/methods ; False Positive Reactions ; Female ; Humans ; Immunoglobulin G/blood ; Immunoglobulin M/blood ; Indonesia ; Male ; Middle Aged ; Nasopharynx/virology ; Point-of-Care Systems ; Reverse Transcriptase Polymerase Chain Reaction/methods ; Sensitivity and Specificity ; Tertiary Care Centers
Chemical Substances	Immunoglobulin G ; Immunoglobulin M
Language	English
Publishing date	2021-03-07
Publishing country	Italy
Document type	Journal Article
ZDB-ID	2394024-4
ISSN	1972-2680 ; 2036-6590
ISSN (online)	1972-2680
ISSN	2036-6590
DOI	10.3855/jidc.13370
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