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  1. Article ; Online: Cryptococcus neoformans VNII as the Main Cause of Cryptococcosis in Domestic Cats from Rio de Janeiro, Brazil

    Rosani Santos Reis / Isabel Cristina Fábregas Bonna / Isabela Maria da Silva Antonio / Sandro Antonio Pereira / Carlos Roberto Sobrinho do Nascimento / Fausto Klabund Ferraris / Fábio Brito-Santos / Isabella Dib Ferreira Gremião / Luciana Trilles

    Journal of Fungi, Vol 7, Iss 980, p

    2021  Volume 980

    Abstract: Cryptococcosis is a systemic fungal disease acquired from contaminated environments with propagules of the basidiomycetous yeasts of the Cryptococcus neoformans and C. gattii species complexes. The C. neoformans species complex classically comprises four ...

    Abstract Cryptococcosis is a systemic fungal disease acquired from contaminated environments with propagules of the basidiomycetous yeasts of the Cryptococcus neoformans and C. gattii species complexes. The C. neoformans species complex classically comprises four major molecular types (VNI, VNII, VNIII, and VNIV), and the C. gattii species complex comprises another four (VGI, VGII, VGIII, and VGIV) and the newly identified molecular type VGV. These major molecular types differ in their epidemiological and ecological features, clinical presentations, and therapeutic outcomes. Generally, the most common isolated types are VNI, VGI, and VGII. The epidemiological profile of cryptococcosis in domestic cats is poorly studied and cats can be the sentinels for human infections. Therefore, the present study aimed to determine the molecular characterization of Cryptococcus spp. isolated from domestic cats and their dwellings in the metropolitan area of Rio de Janeiro, Brazil. A total of 36 Cryptococcus spp. strains, both clinical and environmental, from 19 cats were subtyped using multilocus sequence typing (MLST). The ploidy was identified using flow cytometry and the mating type was determined through amplification with specific pheromone primers. All strains were mating type alpha and 6/36 were diploid (all VNII). Most isolates (63.88%) were identified as VNII, a rare molecular type, leading to the consideration that this genotype is more likely related to skin lesions, since there was a high percentage (68.75%) of cats with skin lesions, which is also considered rare. Further studies regarding the molecular epidemiology of cryptococcosis in felines are still needed to clarify the reason for the large proportion of the rare molecular type VNII causing infections in cats.
    Keywords cryptococcosis ; domestic cats ; MLST ; molecular epidemiology ; Biology (General) ; QH301-705.5
    Subject code 630
    Language English
    Publishing date 2021-11-01T00:00:00Z
    Publisher MDPI AG
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  2. Article ; Online: Importância da inserção de grupo controle em ensaios utilizando animais de laboratório

    Jussara Simmer Bravin / Magno Maciel-Magalhães / Yasmin da Silva Gomes Pinheiro / Miguel Ângelo Brück Gonçalves / Fausto Klabund Ferraris / Fábio Coelho Amendoeira

    Vigilância Sanitária em Debate: Sociedade, Ciência & Tecnologia, Vol 9, Iss

    2021  Volume 1

    Abstract: Introdução: O uso de animais de laboratório é fundamental em áreas em que ainda não podem ser substituídos na totalidade, como a toxicologia. Em estudos que se utilizam, para fins de comparação, apenas dados fornecidos pelos laboratórios de análises ... ...

    Abstract Introdução: O uso de animais de laboratório é fundamental em áreas em que ainda não podem ser substituídos na totalidade, como a toxicologia. Em estudos que se utilizam, para fins de comparação, apenas dados fornecidos pelos laboratórios de análises clínicas, na tentativa de reduzir o número de animais de um experimento, arrisca-se utilizar informações que não correspondem à realidade. Objetivo: Debater o uso de grupo controle em experimentos com animais. Método: Este artigo comparou valores hematológicos e bioquímicos de ratos da linhagem Wistar, obtidos em três biotérios de criação de diferentes estados brasileiros, com os resultados de animais provindos do Instituto de Ciência e Tecnologia em Biomodelos da Fundação Oswaldo Cruz, Rio de Janeiro, objetivando a discussão acerca da tomada de decisão sobre a inclusão de grupo controle em ensaios de toxicidade aguda oral. Resultados: Poucos foram os parâmetros que não apresentaram diferenças estatísticas, tais como: hemácia em fêmeas (duas referências), hemoglobina em fêmeas (uma referência) e machos (uma referência), hematócrito nos machos (duas referências) e hemoglobina corpuscular média nas fêmeas (duas referências), leucócitos nos machos (uma referência), creatinina e alanina aminotransferase (ALT) em todas as fêmeas. Conclusões: O uso do grupo controle se faz necessário quando valores analisados preliminarmente apresentam-se discrepantes daqueles tidos como referenciais da espécie.
    Keywords Animais de Laboratório ; Ratos Wistar ; Experimentação Animal ; Valores de Referência ; Grupo Controle ; Public aspects of medicine ; RA1-1270
    Language English
    Publishing date 2021-02-01T00:00:00Z
    Publisher Fundação Oswaldo Cruz (FIOCRUZ)
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  3. Article ; Online: Author Correction

    Thais Sibioni Berti Bastos / André Guilherme Portela de Paula / Rebeca Bosso dos Santos Luz / Anali M. B. Garnique / Marco A. A. Belo / Silas Fernandes Eto / Dayanne Carla Fernandes / Fausto Klabund Ferraris / Leticia Gomes de Pontes / Tábata Takahashi França / Leonardo José Gil Barcellos / Flavio P. Veras / Pamela Bermejo / Giovanna Guidelli / Carla Maneira / Fellipe da Silveira Bezerra de Mello / Gleidson Teixeira / Gonçalo Amarante Guimarães Pereira / Bianca H. Ventura Fernandes /
    Paulo R. S. Sanches / Helyson Lucas Bezerra Braz / Roberta Jeane Bezerra Jorge / Guilherme Malafaia / Eduardo M. Cilli / Danilo da Silva Olivier / Marcos Serrou do Amaral / Renata J. Medeiros / Antonio Condino-Neto / Luciani R. Carvalho / Glaucia M. Machado-Santelli / Ives Charlie-Silva / Jorge Galindo-Villegas / Tárcio Teodoro Braga

    Scientific Reports, Vol 13, Iss 1, Pp 1-

    A novel insight on SARS-CoV-2 S-derived fragments in the control of the host immunity

    2023  Volume 2

    Keywords Medicine ; R ; Science ; Q
    Language English
    Publishing date 2023-07-01T00:00:00Z
    Publisher Nature Portfolio
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  4. Article ; Online: A novel insight on SARS-CoV-2 S-derived fragments in the control of the host immunity

    Thais Sibioni Berti Bastos / André Guilherme Portela de Paula / Rebeca Bosso dos Santos Luz / Anali M. B. Garnique / Marco A. A. Belo / Silas Fernandes Eto / Dayanne Carla Fernandes / Fausto Klabund Ferraris / Leticia Gomes de Pontes / Tábata Takahashi França / Leonardo José Gil Barcellos / Flavio P. Veras / Pamela Bermejo / Giovanna Guidelli / Carla Maneira / Fellipe da Silveira Bezerra de Mello / Gleidson Teixeira / Gonçalo Amarante Guimarães Pereira / Bianca H. Ventura Fernandes /
    Paulo R. S. Sanches / Helyson Lucas Bezerra Braz / Roberta Jeane Bezerra Jorge / Guilherme Malafaia / Eduardo M. Cilli / Danilo da Silva Olivier / Marcos Serrou do Amaral / Renata J. Medeiros / Antonio Condino-Neto / Luciani R. Carvalho / Glaucia M. Machado-Santelli / Ives Charlie-Silva / Jorge Galindo-Villegas / Tárcio Teodoro Braga

    Scientific Reports, Vol 13, Iss 1, Pp 1-

    2023  Volume 15

    Abstract: Abstract Despite all efforts to combat the pandemic of COVID-19, we are still living with high numbers of infected persons, an overburdened health care system, and the lack of an effective and definitive treatment. Understanding the pathophysiology of ... ...

    Abstract Abstract Despite all efforts to combat the pandemic of COVID-19, we are still living with high numbers of infected persons, an overburdened health care system, and the lack of an effective and definitive treatment. Understanding the pathophysiology of the disease is crucial for the development of new technologies and therapies for the best clinical management of patients. Since the manipulation of the whole virus requires a structure with an adequate level of biosafety, the development of alternative technologies, such as the synthesis of peptides from viral proteins, is a possible solution to circumvent this problem. In addition, the use and validation of animal models is of extreme importance to screen new drugs and to compress the organism's response to the disease. Peptides derived from recombinant S protein from SARS-CoV-2 were synthesized and validated by in silico, in vitro and in vivo methodologies. Macrophages and neutrophils were challenged with the peptides and the production of inflammatory mediators and activation profile were evaluated. These peptides were also inoculated into the swim bladder of transgenic zebrafish larvae at 6 days post fertilization (dpf) to mimic the inflammatory process triggered by the virus, which was evaluated by confocal microscopy. In addition, toxicity and oxidative stress assays were also developed. In silico and molecular dynamics assays revealed that the peptides bind to the ACE2 receptor stably and interact with receptors and adhesion molecules, such as MHC and TCR, from humans and zebrafish. Macrophages stimulated with one of the peptides showed increased production of NO, TNF-α and CXCL2. Inoculation of the peptides in zebrafish larvae triggered an inflammatory process marked by macrophage recruitment and increased mortality, as well as histopathological changes, similarly to what is observed in individuals with COVID-19. The use of peptides is a valuable alternative for the study of host immune response in the context of COVID-19. The use of zebrafish as an animal ...
    Keywords Medicine ; R ; Science ; Q
    Subject code 630
    Language English
    Publishing date 2023-05-01T00:00:00Z
    Publisher Nature Portfolio
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  5. Article: Protective effect of Myrsine parvifolia plant extract against the inflammatory process induced by Bothrops jararaca snake venom

    Corrêa, Arthur Luiz / Adriana Passos Oliveira / Bettina Monika Ruppelt / Erica Ribeiro de Azevedo de Araújo / Marcelo Guerra Santos / Gabriel Rocha Caldas / Flávia Fontenelle Muylaert / Fábio Coelho Amendoeira / Fausto Klabund Ferraris / Claudio Maurício Vieira de Souza / André Lopes Fuly / Leandro Rocha

    Toxicon. 2019 Jan., v. 157

    2019  

    Abstract: Accidents involving snakes from the genus Bothrops sp. constitute the most important cause of snake envenomation in Brazil. The Myrsine genus has been reported to be used in folk medicine against snakebites. In this work, the phytochemical profiles and ... ...

    Abstract Accidents involving snakes from the genus Bothrops sp. constitute the most important cause of snake envenomation in Brazil. The Myrsine genus has been reported to be used in folk medicine against snakebites. In this work, the phytochemical profiles and ability of extracts from Myrsine parvifolia leaves to reduce the inflammatory process (edema, vascular permeability increase and leukocyte migration) induced by Bothrops jararaca venom were investigated in vivo. Chemical compounds were identified by chromatographic and spectroscopy techniques. Total polyphenol, tannin, and flavonoid contents were determined by spectrophotometric methods. Swiss male mice received an oral administration of extracts (100 mg/kg) in different protocols. Paw edema, intraperitoneal vascular permeability and pleurisy models in mice were used to evaluate the antiophidic potential of the extracts. Paw edema was induced by subplantar injection of B. jararaca venom and quantified as the increase in paw volume. Changes in vascular permeability were assessed by measuring the amount of Evans blue dye extravasation. Leukocyte migration was assessed by total and differential counts in the pleural cavity washes. Myricetin, myricetin-3-O-β-arabinopyranoside, quercetin and kaempferol were isolated from the ethyl acetate extract and identified as the primary compounds of the dichloromethane extract. Terpenes and fatty acids were identified in the hexane and dichloromethane extracts. The pretreated group with hydroethanolic and dichloromethane extract reduced total edema (40 and 52%, respectively), vascular permeability increase (32.4 and 32.2%, respectively) and leukocyte influx into the pleural cavity (42 and 39%, respectively), while the group treated with hexane extract showed only reduced edema (37%) induced by B. jararaca venom. The hydroethanolic extract showed better results in all of the tests performed and was also administered by the protocol of post-poisoning, showing maintenance of paw edema reduction and cell migration. These data indicate a potential anti-inflammatory activity of M. parvifolia in poisoning by B. jararaca, especially to reduce local poison effects.
    Keywords Bothrops jararaca ; Myrsine ; accidents ; anti-inflammatory activity ; cell movement ; chromatography ; dyes ; edema ; ethyl acetate ; fatty acids ; hexane ; kaempferol ; laboratory animals ; leaves ; leukocytes ; males ; methylene chloride ; mice ; models ; myricetin ; oral administration ; permeability ; plant extracts ; pleurisy ; poisoning ; polyphenols ; protective effect ; quercetin ; snake bites ; snake venoms ; snakes ; spectroscopy ; terpenoids ; traditional medicine ; Brazil
    Language English
    Dates of publication 2019-01
    Size p. 66-76.
    Publishing place Elsevier Ltd
    Document type Article
    ZDB-ID 204479-1
    ISSN 1879-3150 ; 0041-0101
    ISSN (online) 1879-3150
    ISSN 0041-0101
    DOI 10.1016/j.toxicon.2018.11.289
    Database NAL-Catalogue (AGRICOLA)

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