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  1. Article ; Online: Triladyl® improves the cryopreserved quality and in vivo fertilization potential of Beetal buck (Capra hircus) spermatozoa

    Shah, Syed Aftab Hussain / Haider, Muhammad Shafiq / Ahmed, Hussain / Fayyaz, Muhammad Hammad / Andrabi, Syed Murtaza Hassan

    Small Ruminant Research. 2023 Apr., v. 221 p.106938-

    2023  

    Abstract: This study compared differential concentrations of chicken egg yolk (v/v; T1, 10%; T2, 15%; and T3, 20%) in commercial extender Triladyl® with tris-citric acid based extender (v/v; chicken egg yolk: 20%; C, control) for improving the in vitro quality and ...

    Abstract This study compared differential concentrations of chicken egg yolk (v/v; T1, 10%; T2, 15%; and T3, 20%) in commercial extender Triladyl® with tris-citric acid based extender (v/v; chicken egg yolk: 20%; C, control) for improving the in vitro quality and in vivo fertilization potential of Beetal buck (Capra hircus) spermatozoa at post-thawing. The mean values of progressive motility (PM, %), rapid velocity (RV, %), average path velocity (VAP, μm sec⁻¹), straight line velocity (VSL, μm sec⁻¹), curvilinear velocity (VCL, μm sec⁻¹), supra-vital plasma membrane integrity (SV−PMI, %), mitochondrial transmembrane potential (MMP, %), and viable with intact acrosome (V−IACR, %) were significantly higher(except significantly lower medium velocity; MV, %)in T1 and T2with than control at all stages. Mean values of straightness (STR - VSL: VAP %) and linearity (LIN - VSL: VCL %) were significantly higher in T1 and T2 than control at PD. In vitro longevity, from 0 to 3 h incubation (37 °C), showed significantly low PM (T1) and RV (T1 and T2) percentage loss than control at PT. A significantly positive Pearson's correlation was noticed between CASA and SV−PMI, MMP, V−IACR, and DNA integrity (%). Significantly higher in vivo fertilization rates (%) were recorded in T1 (73.53) and T2 (74.19) compared to the C (46.88) and T3 (48.28), respectively. In conclusion, addition of even chicken egg yolk at 10% (v/v) in Triladyl® improves the cryopreserved in vitro and in vivo quality of Beetal buck spermatozoa as compared to tris-citric acid extender with 20% (v/v) egg yolk.
    Keywords Beetal ; Capra hircus ; DNA ; acrosome ; chickens ; cryopreservation ; egg yolk ; longevity ; membrane potential ; mitochondria ; plasma membrane ; research ; small ruminants ; Beetal buck (Capra hircus) ; Spermatozoa ; Triladyl® ; Tris chicken egg yolkbased extender ; CASA ; Assays ; In vivo fertilization
    Language English
    Dates of publication 2023-04
    Publishing place Elsevier B.V.
    Document type Article ; Online
    ZDB-ID 286928-7
    ISSN 0921-4488
    ISSN 0921-4488
    DOI 10.1016/j.smallrumres.2023.106938
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  2. Article ; Online: Kisspeptin-10 in cryodiluent improves the post-thaw quality of Nili-Ravi buffalo (Bubalus bubalis) bull spermatozoa.

    Fayyaz, Muhammad Hammad / Andrabi, Syed Murtaza Hassan / Haider, Muhammad Shafiq / Khalique, Mubashir Ali / Shah, Syed Aftab Hussain

    Andrologia

    2022  Volume 54, Issue 10, Page(s) e14564

    Abstract: Effects of kisspeptin-10 as antioxidant in cryodiluent were evaluated on post-thaw quality of buffalo spermatozoa. Qualified semen samples from five bulls were pooled, divided into five aliquots and extended in Tris-citric acid cryodiluent containing ... ...

    Abstract Effects of kisspeptin-10 as antioxidant in cryodiluent were evaluated on post-thaw quality of buffalo spermatozoa. Qualified semen samples from five bulls were pooled, divided into five aliquots and extended in Tris-citric acid cryodiluent containing differential doses of kisspeptin-10 (5, 10, 15, and 20 μmol L
    MeSH term(s) Animals ; Antioxidants/pharmacology ; Buffaloes ; Catalase/metabolism ; Citric Acid/pharmacology ; Cryopreservation/veterinary ; Cryoprotective Agents/pharmacology ; Glutathione ; Kisspeptins ; Male ; Semen/metabolism ; Semen Analysis ; Semen Preservation/veterinary ; Sperm Motility ; Spermatozoa
    Chemical Substances Antioxidants ; Cryoprotective Agents ; KISS1 protein, human ; Kisspeptins ; Citric Acid (2968PHW8QP) ; Catalase (EC 1.11.1.6) ; Glutathione (GAN16C9B8O)
    Language English
    Publishing date 2022-08-19
    Publishing country Germany
    Document type Journal Article
    ZDB-ID 7280-1
    ISSN 1439-0272 ; 0303-4569
    ISSN (online) 1439-0272
    ISSN 0303-4569
    DOI 10.1111/and.14564
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    Zs.A 665: Show issues Location:
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  3. Article ; Online: Validation of double freezing protocol for Beetal buck (Capra hircus) spermatozoa.

    Shah, Syed Aftab Hussain / Haider, Muhammad Shafiq / Ahmed, Hussain / Fayyaz, Muhammad Hammad / Andrabi, Syed Murtaza Hassan

    Reproduction in domestic animals = Zuchthygiene

    2022  Volume 57, Issue 12, Page(s) 1615–1622

    Abstract: The study aimed to validate the double versus single freezing protocol for Beetal buck (Capra hircus) spermatozoa in tris-citric acid (TCA) based extender both in terms of quality and fertilization potential. Computer-assisted sperm motion and kinematic ( ...

    Abstract The study aimed to validate the double versus single freezing protocol for Beetal buck (Capra hircus) spermatozoa in tris-citric acid (TCA) based extender both in terms of quality and fertilization potential. Computer-assisted sperm motion and kinematic (CASA) variables, i.e. total (%), and progressive motilities (TM and PM, %) and rapid velocity (RV, %), average path (VAP, μm/s), straight line (VSL, μm/s) and curved line velocities (VCL, μm/s), straightness, (VSL/VAP, %) and linearity, (VSL/VCL, %) as well as supra-vital plasma membrane integrity (SV-PMI, %), mitochondrial membrane potential (MMP, %), viable/intact acrosome (V-IACR, %) and DNA integrity (DNA-I, %) had significantly greater values (p < .05) during single freeze-thawing as compared with the double freeze-thawing at 0, 30, 90, 150 and 210 days, respectively. All CASA and other assays alone did not show significant differences (p > .05) between both freeze-thaw cycles at all treatment durations, respectively. No statistical significance (p > .05) was observed for the in vivo fertility between single (n = 84/141 = 59.72%) and double freeze-thawing (n = 72/136 = 52.9%) cycles, respectively. In conclusion, sperm motion, kinematics, plasma membrane, acrosome, mitochondria and DNA integrities and in vivo fertility are acceptable after the double freezing protocol despite being lower than after one freeze cycle in Beetal buck.
    MeSH term(s) Male ; Animals ; Semen Preservation/veterinary ; Semen Preservation/methods ; Freezing ; Cryoprotective Agents ; Sperm Motility ; Cryopreservation/veterinary ; Cryopreservation/methods ; Semen ; Spermatozoa ; Goats ; DNA
    Chemical Substances Cryoprotective Agents ; DNA (9007-49-2)
    Language English
    Publishing date 2022-09-05
    Publishing country Germany
    Document type Journal Article
    ZDB-ID 1015187-4
    ISSN 1439-0531 ; 0936-6768
    ISSN (online) 1439-0531
    ISSN 0936-6768
    DOI 10.1111/rda.14238
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    In stock of ZB MED Bonn / Germany

    Z 2117: Show issues

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  4. Article ; Online: Validation of double freezing protocol for Beetal buck (Capra hircus) spermatozoa

    Shah, Syed Aftab Hussain / Haider, Muhammad Shafiq / Ahmed, Hussain / Fayyaz, Muhammad Hammad / Andrabi, Syed Murtaza Hassan

    Reproduction in Domestic Animals. 2022 Dec., v. 57, no. 12 p.1615-1622

    2022  

    Abstract: The study aimed to validate the double versus single freezing protocol for Beetal buck (Capra hircus) spermatozoa in tris‐citric acid (TCA) based extender both in terms of quality and fertilization potential. Computer‐assisted sperm motion and kinematic ( ...

    Abstract The study aimed to validate the double versus single freezing protocol for Beetal buck (Capra hircus) spermatozoa in tris‐citric acid (TCA) based extender both in terms of quality and fertilization potential. Computer‐assisted sperm motion and kinematic (CASA) variables, i.e. total (%), and progressive motilities (TM and PM, %) and rapid velocity (RV, %), average path (VAP, μm/s), straight line (VSL, μm/s) and curved line velocities (VCL, μm/s), straightness, (VSL/VAP, %) and linearity, (VSL/VCL, %) as well as supra‐vital plasma membrane integrity (SV‐PMI, %), mitochondrial membrane potential (MMP, %), viable/intact acrosome (V–IACR, %) and DNA integrity (DNA–I, %) had significantly greater values (p < .05) during single freeze–thawing as compared with the double freeze–thawing at 0, 30, 90, 150 and 210 days, respectively. All CASA and other assays alone did not show significant differences (p > .05) between both freeze–thaw cycles at all treatment durations, respectively. No statistical significance (p > .05) was observed for the in vivo fertility between single (n = 84/141 = 59.72%) and double freeze–thawing (n = 72/136 = 52.9%) cycles, respectively. In conclusion, sperm motion, kinematics, plasma membrane, acrosome, mitochondria and DNA integrities and in vivo fertility are acceptable after the double freezing protocol despite being lower than after one freeze cycle in Beetal buck.
    Keywords Beetal ; Capra hircus ; DNA ; acrosome ; freeze-thaw cycles ; kinematics ; membrane potential ; mitochondria ; mitochondrial membrane ; plasma membrane ; reproduction
    Language English
    Dates of publication 2022-12
    Size p. 1615-1622.
    Publishing place John Wiley & Sons, Ltd
    Document type Article ; Online
    Note JOURNAL ARTICLE
    ZDB-ID 1015187-4
    ISSN 1439-0531 ; 0936-6768
    ISSN (online) 1439-0531
    ISSN 0936-6768
    DOI 10.1111/rda.14238
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    In stock of ZB MED Bonn / Germany

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  5. Article ; Online: Antioxidant effects of zinc-oxide nanoparticles on post-thaw quality and in vivo fertility of Beetal buck spermatozoa

    Khalique, Mubashir Ali / Rehman, Habib / Andrabi, Syed Murtaza Hassan / Majeed, Khalid Abdul / Ahmad, Nisar / Fayyaz, Muhammad Hammad / Haider, Muhammad Shafiq / Naz, Syeda Sohaila / Qureshi, Irfan Zia / Sulaiman, Sulaiman

    Small Ruminant Research. 2023 May 23, p.107012-

    2023  , Page(s) 107012–

    Abstract: Metallic nanoparticles are generally considered potential cryoprotectants during semen cryopreservation owing to their ability to improve the spermatozoa quality following cryopreservation when added in cryo-diluents. The present study aimed at ... ...

    Abstract Metallic nanoparticles are generally considered potential cryoprotectants during semen cryopreservation owing to their ability to improve the spermatozoa quality following cryopreservation when added in cryo-diluents. The present study aimed at investigating the effects of incorporating zinc oxide nanoparticles (ZnO-NPs) in the cryo-diluent on in vitro post-thaw sperm quality attributes, oxidant-antioxidant profile, and in vivo fertility rate in Beetal buck. After characterization, ZnO-NPs were added to the Tris-citric acid-fructose yolk extender. Semen samples, collected from five breeding bucks (5 ejaculates per buck) using an artificial vagina, were pooled. The pooled semen samples, distributed into five equal aliquots, were diluted in the Tris-citric acid cryo-diluent containing five different concentrations of ZnO-NPs (0.5mg/ml, 1.0mg/ml, 1.5mg/ml, 2.0mg/ml, and 0.0mg/ml called control group), and were cryopreserved for 24hours. At post-thaw, various in vitro sperm quality parameters, and antioxidant enzyme profiles like superoxide dismutase (SOD), catalase (CAT), peroxidase (POD), and reduced glutathione (GSH) levels were determined. For the in vivo fertility experiment, breeding does (n = 152), aging from 1.5 to 4 years, were artificially inseminated with spermatozoa with intact acrosome containing the same five concentrations of ZnO-NPs. Results demonstrated that the 1.0mg/ml ZnO-NPs (crystallite size of 19.73nm) improved (p < 0.01) the activities of antioxidant enzymes (SOD, CAT, and POD) with concomitantly decreased levels (p < 0.001) of lipid peroxidation (LPO) and reactive oxygen species (ROS) when compared with the other groups. Total spermatozoa motility, rapid velocity, and average path velocity were higher (p < 0.05) with the 1.0mg/ml ZnO-NPs when compared with the control group. Progressive motility and straight-line velocity were higher (p < 0.05) with the 0.5, 1.0, and 1.5mg/ml of ZnO-NPs addition compared with the 2.0mg/ml and control groups. The sperm kinematics parameters; the amplitude of the lateral head displacement, the beat cross frequency, the straightness, and the linearity were not influenced by the ZnO-NPs inclusion. Supra-vital plasma membrane integrity was higher (p < 0.001) with the 0.5mg/ml and 1.0mg/ml of ZnO-NPs added groups when compared with either 2.0mg/ml ZnO-NPs or the control group. The viable sperm with intact acrosome was higher (p < 0.05) in the 1.0mg/ml ZnO-NPs group compared with the other groups. Low supplementation rates improved (p < 0.05) the DNA integrity when compared with the higher dose (2.0mg/ml ZnO-NPs). The pregnancy per AI was higher (59.3%; P < 0.05) in the does inseminated with semen added with the 0.5mg/ml ZnO-NPs followed by 1.0mg/ml (58.8%), 1.5mg/ml (58.6%), and 2.0mg/ml (46.6%) when compared with the control group (33.3%). We concluded that the 1.0mg/ml ZnO-NPs added in the citric acid-fructose yolk extender improved the post-thaw quality of Beetal buck spermatozoa in terms of elevated activities of endogenous antioxidant enzymes, improved structural and functional integrity along with higher fertility.
    Keywords Beetal ; DNA ; acrosome ; antioxidant enzymes ; artificial vagina ; catalase ; cryopreservation ; cryoprotectants ; crystallites ; glutathione ; head ; kinematics ; lipid peroxidation ; nanoparticles ; peroxidase ; plasma membrane ; pregnancy ; reactive oxygen species ; research ; semen ; small ruminants ; sperm quality ; superoxide dismutase ; zinc oxide ; zinc oxide nanoparticles ; Bucks spermatozoa ; antioxidants ; sperm motility ; artificial insemination ; fertility
    Language English
    Dates of publication 2023-0523
    Publishing place Elsevier B.V.
    Document type Article ; Online
    Note Pre-press version
    ZDB-ID 286928-7
    ISSN 0921-4488
    ISSN 0921-4488
    DOI 10.1016/j.smallrumres.2023.107012
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    In stock of ZB MED Bonn / Germany

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  6. Article ; Online: Cerium oxide nanoparticles improve the post-thaw quality and in-vivo fertility of Beetal buck spermatozoa.

    Khalique, Mubashir Ali / Andrabi, Syed Murtaza Hassan / Majeed, Khalid Abdul / Yousaf, Muhammad Shahbaz / Ahmad, Nisar / Tahir, Sajid Khan / Fayyaz, Muhammad Hammad / Haider, Muhammad Shafiq / Naz, Syeda Sohaila / Qureshi, Irfan Zia / Sulaiman, Sulaiman / Zaneb, Hafsa / Rehman, Habib

    Theriogenology

    2023  Volume 214, Page(s) 166–172

    Abstract: The motility, health quality, and membrane disorders of spermatozoa are adversely affected during the process of semen cryopreservation due to the over-production of reactive oxygen species (ROS). Cerium oxide nanoparticles ( ... ...

    Abstract The motility, health quality, and membrane disorders of spermatozoa are adversely affected during the process of semen cryopreservation due to the over-production of reactive oxygen species (ROS). Cerium oxide nanoparticles (CeO
    MeSH term(s) Pregnancy ; Female ; Male ; Animals ; Semen/metabolism ; Antioxidants/pharmacology ; Semen Analysis/veterinary ; Reactive Oxygen Species/pharmacology ; Sperm Motility ; Cryoprotective Agents/pharmacology ; Semen Preservation/veterinary ; Semen Preservation/methods ; Spermatozoa ; Cryopreservation/veterinary ; Cryopreservation/methods ; Fertility ; Citrates/pharmacology
    Chemical Substances ceric oxide (619G5K328Y) ; Antioxidants ; Reactive Oxygen Species ; Cryoprotective Agents ; Citrates
    Language English
    Publishing date 2023-10-20
    Publishing country United States
    Document type Journal Article
    ZDB-ID 189232-0
    ISSN 1879-3231 ; 0093-691X
    ISSN (online) 1879-3231
    ISSN 0093-691X
    DOI 10.1016/j.theriogenology.2023.10.022
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    In stock of ZB MED Cologne/Königswinter

    Zs.A 3929: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (2.OG)
    ab Jg. 2022: Lesesaal (EG)

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