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  1. Article: Endovascular treatment of a critical common iliac artery stenosis using intravascular ultrasound guidance in a patient with a known severe iodine contrast allergy.

    Fee, Charles / Lewis, Katharine

    Ultrasound (Leeds, England)

    2023  Volume 31, Issue 4, Page(s) 308–311

    Abstract: Introduction: The mainstay of endovascular treatment in peripheral arterial disease is digital subtraction angiography with an iodinated contrast. In patients with a known contrast allergy, the current most commonly used alternative is to perform carbon ...

    Abstract Introduction: The mainstay of endovascular treatment in peripheral arterial disease is digital subtraction angiography with an iodinated contrast. In patients with a known contrast allergy, the current most commonly used alternative is to perform carbon dioxide angiography. Operator experience and availability of carbon dioxide (CO
    Case report: We present a case describing the use of intravascular ultrasound and fluoroscopy to guide endovascular treatment of a critical left common iliac artery stenosis in a patient with known anaphylaxis to iodinated contrast.
    Discussion: Intravascular ultrasound is fast becoming the gold standard treatment option in peripheral venous interventions; however, the use in peripheral arterial disease is limited. We believe this is the first case that reports the use of predominantly intravascular ultrasound in endovascular peripheral arterial intervention with a contrast adjunct.
    Conclusion: We have demonstrated that intravascular ultrasound is a safe and effective alternative or adjunct to iodinated contrast for arterial angiography and stenting in the setting of patient contra-indications.
    Language English
    Publishing date 2023-01-27
    Publishing country United States
    Document type Case Reports
    ZDB-ID 2298926-2
    ISSN 1743-1344 ; 1742-271X
    ISSN (online) 1743-1344
    ISSN 1742-271X
    DOI 10.1177/1742271X221147721
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 7025: Show issues Location:
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  2. Article ; Online: Ultrasound assessment of the hamstrings complex of athletes with MRI correlation.

    Fee, C / Sergot, L / Kho, J / Chakraverty, J

    Clinical radiology

    2022  Volume 77, Issue 5, Page(s) 337–344

    Abstract: Due to anatomical complexity, ultrasound examination of the hamstring muscles is challenging, resulting in potential diagnostic uncertainty and under-confidence in the technique. This leads to a subsequent tendency to favour magnetic resonance imaging ( ... ...

    Abstract Due to anatomical complexity, ultrasound examination of the hamstring muscles is challenging, resulting in potential diagnostic uncertainty and under-confidence in the technique. This leads to a subsequent tendency to favour magnetic resonance imaging (MRI) evaluation, which can delay diagnosis and potential intervention. This article describes a comprehensive technique of ultrasound evaluation of the hamstrings complex, using key anatomical landmarks. A direct comparison of the sonographic landmarks with corresponding MRI appearances is also provided. If these landmarks can be identified successfully, the complex anatomy can be unlocked, thus improving the time and sensitivity of diagnosis of acute injuries, as well as providing a reliable framework for monitoring injury progress and helping to identify candidates for potential intervention. Many of the anatomical landmarks discussed are common areas of injury in elite athletes, encountered frequently in clinical practice.
    MeSH term(s) Athletes ; Athletic Injuries/diagnostic imaging ; Hamstring Muscles/diagnostic imaging ; Humans ; Magnetic Resonance Imaging/methods ; Ultrasonography
    Language English
    Publishing date 2022-02-28
    Publishing country England
    Document type Journal Article ; Review
    ZDB-ID 391227-9
    ISSN 1365-229X ; 0009-9260
    ISSN (online) 1365-229X
    ISSN 0009-9260
    DOI 10.1016/j.crad.2022.01.049
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  3. Article ; Online: Performance of various 3D-printed monolith geometries as an alternative to expanded bed adsorption for protein purification.

    Pei, Yuanjun / Feast, Sean / Holland, Daniel / Fee, Conan

    Biotechnology and bioengineering

    2023  

    Abstract: Here, we compare the performance of various three-dimensional-printed Monolith Adsorption (PMA) columns designed from a triply periodic minimal surface geometry, the Schoen gyroid. The structures examined had designed hydraulic diameters between 203 and ... ...

    Abstract Here, we compare the performance of various three-dimensional-printed Monolith Adsorption (PMA) columns designed from a triply periodic minimal surface geometry, the Schoen gyroid. The structures examined had designed hydraulic diameters between 203 and 458 µm and voidages of 40%-60%. We compare column efficiency, porosity, static binding capacity and dynamic binding capacity for various load volumes and flow rates. The results show that all structures allowed efficient passage of yeast cells (>97%) over a wide range of interstitial velocities (191 to 1911 cm/h) while maintaining a low pressure drop (<0.1 MPa). The structure with a voidage of 40% and a hydraulic diameter of 203 µm showed the best performance in all aspects evaluated. Bovine serum albumin (BSA) recoveries for all structures (27%-91% when the loaded volume was 180 mL) were significantly affected by hydraulic diameter, mean channel wall thickness, velocity and voidage. Moreover, biomass addition resulted in a decrease in BSA recovery, which became more obvious at high velocities. However, this did not lead to a dramatic reduction in saturated binding capacity, significant changes in axial dispersion, or blockage of channels and could be compensated for by recirculation of the feed, even at high velocity. PMA thus potentially provides an appealing alternative to Expanded Bed Adsorption, retaining the latter's advantages, while eliminating fluidization issues and minimizing both processing time and buffer consumption.
    Language English
    Publishing date 2023-04-11
    Publishing country United States
    Document type Journal Article
    ZDB-ID 280318-5
    ISSN 1097-0290 ; 0006-3592
    ISSN (online) 1097-0290
    ISSN 0006-3592
    DOI 10.1002/bit.28398
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  4. Article ; Online: Growth phase-dependent surface properties of Legionella pneumophila and their role in adhesion to stainless steel coated QCM-D sensors.

    Ariyadasa, S / Abeysekera, G / Billington, C / Fee, C / Pang, L

    Letters in applied microbiology

    2021  Volume 73, Issue 2, Page(s) 257–267

    Abstract: Legionella pneumophila cell surface hydrophobicity and charge are important determinants of their mobility and persistence in engineered water systems (EWS). These surface properties may differ depending on the growth phase of L. pneumophila resulting in ...

    Abstract Legionella pneumophila cell surface hydrophobicity and charge are important determinants of their mobility and persistence in engineered water systems (EWS). These surface properties may differ depending on the growth phase of L. pneumophila resulting in variable adhesion and persistence within EWS. We describe the growth-dependent variations in L. pneumophila cell surface hydrophobicity and surface charge using the microbial adhesion to hydrocarbon assay and microelectrophoresis, respectively, and their role in cell adhesion to stainless steel using a quartz crystal microbalance with dissipation (QCM-D) monitoring instrument. We observed a steady increase in L. pneumophila hydrophobicity during their lifecycle in culture media. Cell surfaces of stationary phase L. pneumophila were significantly more hydrophobic than their lag and midexponential counterparts. No significant changes in L. pneumophila cell surface charge were noted. Morphology of L. pneumophila remained relatively constant throughout their lifecycle. In the QCM-D study, lag and exponential phase L. pneumophila weakly adhered to stainless steel surfaces resulting in viscoelastic layers. In contrast, stationary phase bacteria were tightly and irreversibly bound to the surfaces, forming rigid layers. Our results suggest that the stationary phase of L. pneumophila would highly favour their adhesion to plumbing surfaces and persistence in EWS.
    MeSH term(s) Bacterial Adhesion ; Hydrophobic and Hydrophilic Interactions ; Legionella pneumophila/physiology ; Legionella pneumophila/ultrastructure ; Microscopy, Electron, Scanning ; Quartz Crystal Microbalance Techniques ; Stainless Steel ; Surface Properties
    Chemical Substances Stainless Steel (12597-68-1)
    Language English
    Publishing date 2021-06-03
    Publishing country England
    Document type Journal Article
    ZDB-ID 632584-1
    ISSN 1472-765X ; 0266-8254
    ISSN (online) 1472-765X
    ISSN 0266-8254
    DOI 10.1111/lam.13510
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article ; Online: Printed monolith adsorption as an alternative to expanded bed adsorption for purifying M13 bacteriophage.

    Feast, Sean / Fee, Conan / Huber, Tim / Clarke, Daniel

    Journal of chromatography. A

    2021  Volume 1652, Page(s) 462365

    Abstract: An ordered 3D printed chromatography stationary phase was used to purify M13 bacteriophage (M13) directly from crude cell culture. This new approach, which offers the same advantages as expanded bed adsorption (EBA) with regard to tolerating solids-laden ...

    Abstract An ordered 3D printed chromatography stationary phase was used to purify M13 bacteriophage (M13) directly from crude cell culture. This new approach, which offers the same advantages as expanded bed adsorption (EBA) with regard to tolerating solids-laden feed streams but without the corresponding issues associated with fluidized bed stability that affect the latter, can be described as "printed monolith adsorption (PMA)". PMA columns (5, 10 and 15 cm length by 1 cm diameter) were made via a wax templating method from cross-linked cellulose hydrogel and functionalized with a quaternary amine ligand. The recovery of M13 was found to be strongly linked to load flow rate, with the highest recovery 89.7% ± 6% for 1.4 × 10
    MeSH term(s) Adsorption ; Bacteriophage M13/isolation & purification ; Culture Media ; Virology/instrumentation ; Virology/methods
    Chemical Substances Culture Media
    Language English
    Publishing date 2021-06-29
    Publishing country Netherlands
    Document type Journal Article
    ZDB-ID 1171488-8
    ISSN 1873-3778 ; 0021-9673
    ISSN (online) 1873-3778
    ISSN 0021-9673
    DOI 10.1016/j.chroma.2021.462365
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  6. Article ; Online: Evaluation of Biopolymer Materials and Synthesis Techniques to Develop a Rod-Shaped Biopolymer Surrogate for

    Ariyadasa, Sujani / Daear, Weiam / Abeysekera, Gayan / Billington, Craig / Fee, Conan / Prenner, Elmar / Pang, Liping

    Polymers

    2022  Volume 14, Issue 13

    Abstract: Biopolymer microparticles have been developed for applications that require biocompatibility and biodegradability, such as drug delivery. In this study, we assessed the production of microparticles using carnauba wax, κ-carrageenan, alginate, and poly ( ... ...

    Abstract Biopolymer microparticles have been developed for applications that require biocompatibility and biodegradability, such as drug delivery. In this study, we assessed the production of microparticles using carnauba wax, κ-carrageenan, alginate, and poly (lactic-co-glycolic acid) (PLGA) with the aim of developing a novel, DNA-tracer-loaded, biopolymer surrogate with a size, shape, surface charge, and relative hydrophobicity similar to stationary-phase
    Language English
    Publishing date 2022-06-24
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2527146-5
    ISSN 2073-4360 ; 2073-4360
    ISSN (online) 2073-4360
    ISSN 2073-4360
    DOI 10.3390/polym14132571
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  7. Article ; Online: Use of a Novel DNA-Loaded Alginate-Calcium Carbonate Biopolymer Surrogate to Study the Engulfment of Legionella pneumophila by Acanthamoeba polyphaga in Water Systems.

    Ariyadasa, Sujani / Billington, Craig / Shaheen, Mohamed / Ashbolt, Nicholas J / Fee, Conan / Pang, Liping

    Microbiology spectrum

    2022  Volume 10, Issue 4, Page(s) e0221022

    Abstract: The engulfment of Legionella pneumophila by free-living amoebae (FLA) in engineered water systems (EWS) enhances L. pneumophila persistence and provides a vehicle for rapid replication and increased public health risk. Despite numerous legionellosis ... ...

    Abstract The engulfment of Legionella pneumophila by free-living amoebae (FLA) in engineered water systems (EWS) enhances L. pneumophila persistence and provides a vehicle for rapid replication and increased public health risk. Despite numerous legionellosis outbreaks worldwide, effective tools for studying interactions between L. pneumophila and FLA in EWS are lacking. To address this, we have developed a biopolymer surrogate with a similar size, shape, surface charge, and hydrophobicity to those of stationary-phase L. pneumophila. Parallel experiments were conducted to observe the engulfment of L. pneumophila and the surrogate by Acanthamoeba polyphaga in dechlorinated, filter-sterilised tap water at 30°C for 72 h. Trophozoites engulfed both the surrogate and L. pneumophila, reaching maximum uptake after 2 and 6 h, respectively, but the peak surrogate uptake was ~2-log lower. Expulsion of the engulfed surrogate from
    MeSH term(s) Acanthamoeba/microbiology ; Alginates ; Biopolymers ; Calcium Carbonate ; DNA ; Humans ; Legionella pneumophila/genetics ; Water
    Chemical Substances Alginates ; Biopolymers ; Water (059QF0KO0R) ; DNA (9007-49-2) ; Calcium Carbonate (H0G9379FGK)
    Language English
    Publishing date 2022-08-11
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2807133-5
    ISSN 2165-0497 ; 2165-0497
    ISSN (online) 2165-0497
    ISSN 2165-0497
    DOI 10.1128/spectrum.02210-22
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  8. Article ; Online: Pro: should evidence-based medicine be used more in clinical practice?

    Fee, Chris

    The California journal of emergency medicine

    2010  Volume 7, Issue 1, Page(s) 13–16

    Language English
    Publishing date 2010-05-22
    Publishing country United States
    Document type Journal Article
    ZDB-ID 2589126-1
    ISSN 1948-3392 ; 1948-3392
    ISSN (online) 1948-3392
    ISSN 1948-3392
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  9. Article ; Online: Rebuttal of con.

    Fee, Chris

    The California journal of emergency medicine

    2010  Volume 7, Issue 1, Page(s) 19–20

    Language English
    Publishing date 2010-05-22
    Publishing country United States
    Document type Journal Article
    ZDB-ID 2589126-1
    ISSN 1948-3392 ; 1948-3392
    ISSN (online) 1948-3392
    ISSN 1948-3392
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  10. Article: Prophylactic Efficacy of Riluzole against Anxiety- and Depressive-Like Behaviors in Two Rodent Stress Models.

    Bansal, Yashika / Fee, Corey / Misquitta, Keith A / Codeluppi, Sierra A / Sibille, Etienne / Berman, Robert M / Coric, Vladimir / Sanacora, Gerard / Banasr, Mounira

    Complex psychiatry

    2023  Volume 9, Issue 1-4, Page(s) 57–69

    Abstract: Introduction: Chronic stress-related illnesses such as major depressive disorder and post-traumatic stress disorder share symptomatology, including anxiety, anhedonia, and helplessness. Across disorders, neurotoxic dysregulated glutamate (Glu) signaling ...

    Abstract Introduction: Chronic stress-related illnesses such as major depressive disorder and post-traumatic stress disorder share symptomatology, including anxiety, anhedonia, and helplessness. Across disorders, neurotoxic dysregulated glutamate (Glu) signaling may underlie symptom emergence. Current first-line antidepressant drugs, which do not directly target Glu signaling, fail to provide adequate benefit for many patients and are associated with high relapse rates. Riluzole modulates glutamatergic neurotransmission by increasing metabolic cycling and modulating signal transduction. Clinical studies exploring riluzole's efficacy in stress-related disorders have provided varied results. However, the utility of riluzole for treating specific symptom dimensions or as a prophylactic treatment has not been comprehensively assessed.
    Methods: We investigated whether chronic prophylactic riluzole (∼12-15 mg/kg/day p.o.) could prevent the emergence of behavioral deficits induced by unpredictable chronic mild stress (UCMS) in mice. We assessed (i) anxiety-like behavior using the elevated-plus maze, open-field test, and novelty-suppressed feeding, (ii) mixed anxiety/anhedonia-like behavior in the novelty-induced hypophagia test, and (iii) anhedonia-like behavior using the sucrose consumption test. Z-scoring summarized changes across tests measuring similar dimensions. In a separate learned helplessness (LH) cohort, we investigated whether chronic prophylactic riluzole treatment could block the development of helplessness-like behavior.
    Results: UCMS induced an elevation in anhedonia-like behavior and overall behavioral emotionality that was blocked by prophylactic riluzole. In the LH cohort, prophylactic riluzole blocked the development of helplessness-like behavior.
    Discussion/conclusion: This study supports the utility of riluzole as a prophylactic medication for preventing anhedonia and helplessness symptoms associated with stress-related disorders.
    Language English
    Publishing date 2023-02-03
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 3021167-0
    ISSN 2673-298X ; 2673-3005
    ISSN (online) 2673-298X
    ISSN 2673-3005
    DOI 10.1159/000529534
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