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  1. Article: Serological evidence of exposure to ebolaviruses in domestic pigs from Guinea

    Fischer, Kerstin / Camara, Alimou / Troupin, Cécile / Fehling, Sarah K / Strecker, Thomas / Groschup, Martin H / Tordo, Noel / Diederich, Sandra

    Transboundary and emerging diseases. 2020 Mar., v. 67, no. 2

    2020  

    Abstract: The genus Ebolavirus comprises several virus species with zoonotic potential and varying pathogenicity for humans. Ebolaviruses are considered to circulate in wildlife with occasional spillover events into the human population which then often leads to ... ...

    Abstract The genus Ebolavirus comprises several virus species with zoonotic potential and varying pathogenicity for humans. Ebolaviruses are considered to circulate in wildlife with occasional spillover events into the human population which then often leads to severe disease outbreaks. Several studies indicate a significant role of bats as reservoir hosts in the ebolavirus ecology. However, pigs from the Philippines have been found to be naturally infected with Reston virus (RESTV), an ebolavirus that is thought to only cause asymptomatic infections in humans. The recent report of ebolavirus‐specific antibodies in pigs from Sierra Leone further supports natural infection of pigs with ebolaviruses. However, susceptibility of pigs to highly pathogenic Ebola virus (EBOV) was only shown under experimental settings and evidence for natural infection of pigs with EBOV is currently lacking. Between October and December 2017, we collected 308 serum samples from pigs in Guinea, West Africa, and tested for the presence of ebolavirus‐specific antibodies with different serological assays. Besides reactivity to EBOV nucleoproteins in ELISA and Western blot for 19 (6.2%) and 13 (4.2%) samples, respectively, four sera recognized Sudan virus (SUDV) NP in Western blot. Furthermore, four samples specifically detected EBOV or SUDV glycoprotein (GP) in an indirect immunofluorescence assay under native conditions. Virus neutralization assay based on EBOV (Mayinga isolate) revealed five weakly neutralizing sera. The finding of (cross‐) reactive and weakly neutralizing antibodies suggests the exposure of pigs from Guinea to ebolaviruses or ebola‐like viruses with their pathogenicity as well as their zoonotic potential remaining unknown. Future studies should investigate whether pigs can act as an amplifying host for ebolaviruses and whether there is a risk for spillover events.
    Keywords Chiroptera ; Ebolavirus ; Western blotting ; blood serum ; disease outbreaks ; disease reservoirs ; disease severity ; ecology ; emerging diseases ; enzyme-linked immunosorbent assay ; fluorescent antibody technique ; glycoproteins ; human population ; humans ; neutralization ; neutralization tests ; neutralizing antibodies ; nucleoproteins ; pathogenicity ; risk ; swine ; swine diseases ; viruses ; wildlife ; zoonoses ; Guinea ; Philippines ; Sierra Leone ; Sudan
    Language English
    Dates of publication 2020-03
    Size p. 724-732.
    Publishing place John Wiley & Sons, Ltd
    Document type Article
    Note JOURNAL ARTICLE
    ZDB-ID 2414822-2
    ISSN 1865-1682 ; 1865-1674
    ISSN (online) 1865-1682
    ISSN 1865-1674
    DOI 10.1111/tbed.13391
    Database NAL-Catalogue (AGRICOLA)

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  2. Article ; Online: Proteomic landscape of SARS-CoV-2- and MERS-CoV-infected primary human renal epithelial cells.

    Kohli, Aneesha / Sauerhering, Lucie / Fehling, Sarah K / Klann, Kevin / Geiger, Helmut / Becker, Stephan / Koch, Benjamin / Baer, Patrick C / Strecker, Thomas / Münch, Christian

    Life science alliance

    2022  Volume 5, Issue 5

    Abstract: Acute kidney injury is associated with mortality in COVID-19 patients. However, host cell changes underlying infection of renal cells with SARS-CoV-2 remain unknown and prevent understanding of the molecular mechanisms that may contribute to renal ... ...

    Abstract Acute kidney injury is associated with mortality in COVID-19 patients. However, host cell changes underlying infection of renal cells with SARS-CoV-2 remain unknown and prevent understanding of the molecular mechanisms that may contribute to renal pathology. Here, we carried out quantitative translatome and whole-cell proteomics analyses of primary renal proximal and distal tubular epithelial cells derived from human donors infected with SARS-CoV-2 or MERS-CoV to disseminate virus and cell type-specific changes over time. Our findings revealed shared pathways modified upon infection with both viruses, as well as SARS-CoV-2-specific host cell modulation driving key changes in innate immune activation and cellular protein quality control. Notably, MERS-CoV infection-induced specific changes in mitochondrial biology that were not observed in response to SARS-CoV-2 infection. Furthermore, we identified extensive modulation in pathways associated with kidney failure that changed in a virus- and cell type-specific manner. In summary, we provide an overview of the effects of SARS-CoV-2 or MERS-CoV infection on primary renal epithelial cells revealing key pathways that may be essential for viral replication.
    MeSH term(s) Biomarkers ; COVID-19/metabolism ; COVID-19/virology ; Cell Nucleus/genetics ; Cell Nucleus/metabolism ; Cells, Cultured ; Computational Biology/methods ; Coronavirus Infections/metabolism ; Coronavirus Infections/virology ; Epithelial Cells/metabolism ; Epithelial Cells/virology ; Gene Expression Regulation ; Host-Pathogen Interactions/genetics ; Host-Pathogen Interactions/immunology ; Humans ; Kidney ; Kidney Tubules, Distal ; Kidney Tubules, Proximal ; Middle East Respiratory Syndrome Coronavirus/physiology ; Mitochondria/genetics ; Mitochondria/metabolism ; Primary Cell Culture ; Proteome ; Proteomics/methods ; SARS-CoV-2/physiology ; Virus Replication
    Chemical Substances Biomarkers ; Proteome
    Language English
    Publishing date 2022-02-02
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ISSN 2575-1077
    ISSN (online) 2575-1077
    DOI 10.26508/lsa.202201371
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article ; Online: Serological evidence of exposure to ebolaviruses in domestic pigs from Guinea.

    Fischer, Kerstin / Camara, Alimou / Troupin, Cécile / Fehling, Sarah K / Strecker, Thomas / Groschup, Martin H / Tordo, Noel / Diederich, Sandra

    Transboundary and emerging diseases

    2019  Volume 67, Issue 2, Page(s) 724–732

    Abstract: The genus Ebolavirus comprises several virus species with zoonotic potential and varying pathogenicity for humans. Ebolaviruses are considered to circulate in wildlife with occasional spillover events into the human population which then often leads to ... ...

    Abstract The genus Ebolavirus comprises several virus species with zoonotic potential and varying pathogenicity for humans. Ebolaviruses are considered to circulate in wildlife with occasional spillover events into the human population which then often leads to severe disease outbreaks. Several studies indicate a significant role of bats as reservoir hosts in the ebolavirus ecology. However, pigs from the Philippines have been found to be naturally infected with Reston virus (RESTV), an ebolavirus that is thought to only cause asymptomatic infections in humans. The recent report of ebolavirus-specific antibodies in pigs from Sierra Leone further supports natural infection of pigs with ebolaviruses. However, susceptibility of pigs to highly pathogenic Ebola virus (EBOV) was only shown under experimental settings and evidence for natural infection of pigs with EBOV is currently lacking. Between October and December 2017, we collected 308 serum samples from pigs in Guinea, West Africa, and tested for the presence of ebolavirus-specific antibodies with different serological assays. Besides reactivity to EBOV nucleoproteins in ELISA and Western blot for 19 (6.2%) and 13 (4.2%) samples, respectively, four sera recognized Sudan virus (SUDV) NP in Western blot. Furthermore, four samples specifically detected EBOV or SUDV glycoprotein (GP) in an indirect immunofluorescence assay under native conditions. Virus neutralization assay based on EBOV (Mayinga isolate) revealed five weakly neutralizing sera. The finding of (cross-) reactive and weakly neutralizing antibodies suggests the exposure of pigs from Guinea to ebolaviruses or ebola-like viruses with their pathogenicity as well as their zoonotic potential remaining unknown. Future studies should investigate whether pigs can act as an amplifying host for ebolaviruses and whether there is a risk for spillover events.
    MeSH term(s) Animals ; Antibodies, Neutralizing/blood ; Antibodies, Viral/blood ; Cross Reactions ; Disease Outbreaks/veterinary ; Disease Reservoirs/veterinary ; Ebolavirus/immunology ; Ebolavirus/isolation & purification ; Enzyme-Linked Immunosorbent Assay/veterinary ; Farms ; Female ; Guinea/epidemiology ; Hemorrhagic Fever, Ebola/epidemiology ; Hemorrhagic Fever, Ebola/veterinary ; Hemorrhagic Fever, Ebola/virology ; Humans ; Immunoglobulin G/blood ; Male ; Nucleoproteins/immunology ; Sus scrofa ; Swine ; Swine Diseases/epidemiology ; Swine Diseases/virology
    Chemical Substances Antibodies, Neutralizing ; Antibodies, Viral ; Immunoglobulin G ; Nucleoproteins
    Language English
    Publishing date 2019-11-02
    Publishing country Germany
    Document type Journal Article
    ZDB-ID 2414822-2
    ISSN 1865-1682 ; 1865-1674
    ISSN (online) 1865-1682
    ISSN 1865-1674
    DOI 10.1111/tbed.13391
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article ; Online: CP100356 Hydrochloride, a P-Glycoprotein Inhibitor, Inhibits Lassa Virus Entry

    Takenaga, Toru / Zhang, Zihan / Muramoto, Yukiko / Fehling, Sarah K. / Hirabayashi, Ai / Takamatsu, Yuki / Kajikawa, Junichi / Miyamoto, Sho / Nakano, Masahiro / Urata, Shuzo / Groseth, Allison / Strecker, Thomas / Noda, Takeshi

    Implication of a Candidate Pan-Mammarenavirus Entry Inhibitor

    2021  

    Abstract: Lassa virus (LASV)—a member of the family Arenaviridae—causes Lassa fever in humans and is endemic in West Africa. Currently, no approved drugs are available. We screened 2480 small compounds for their potential antiviral activity using pseudotyped ... ...

    Abstract Lassa virus (LASV)—a member of the family Arenaviridae—causes Lassa fever in humans and is endemic in West Africa. Currently, no approved drugs are available. We screened 2480 small compounds for their potential antiviral activity using pseudotyped vesicular stomatitis virus harboring the LASV glycoprotein (VSV-LASVGP) and a related prototypic arenavirus, lymphocytic choriomeningitis virus (LCMV). Follow-up studies confirmed that CP100356 hydrochloride (CP100356), a specific P-glycoprotein (P-gp) inhibitor, suppressed VSV-LASVGP, LCMV, and LASV infection with half maximal inhibitory concentrations of 0.52, 0.54, and 0.062 μM, respectively, without significant cytotoxicity. Although CP100356 did not block receptor binding at the cell surface, it inhibited low-pH-dependent membrane fusion mediated by arenavirus glycoproteins. P-gp downregulation did not cause a significant reduction in either VSV-LASVGP or LCMV infection, suggesting that P-gp itself is unlikely to be involved in arenavirus entry. Finally, our data also indicate that CP100356 inhibits the infection by other mammarenaviruses. Thus, our findings suggest that CP100356 can be considered as an effective virus entry inhibitor for LASV and other highly pathogenic mammarenaviruses.
    Keywords Text ; ddc:570 ; Lassa virus -- lymphocytic choriomeningitis virus -- arenavirus -- pseudotyped vesicular stomatitis virus -- entry inhibitor
    Subject code 570
    Language English
    Publishing date 2021-09-03
    Publishing country de
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  5. Article ; Online: Serological Evidence of Exposure to Ebolaviruses in Domestic Pigs from Guinea

    Fischer, Kerstin / Troupin, Cécile / Tordo, Noel / Fehling, Sarah K. / Groschup, Martin H. / Strecker, Thomas / Camara, Alimou / Diederich, Sandra

    2019  

    Abstract: The genus Ebolavirus comprises several virus species with zoonotic potential and varying pathogenicity for humans. Ebolaviruses are considered to circulate in wildlife with occasional spillover events into the human population which then often leads to ... ...

    Abstract The genus Ebolavirus comprises several virus species with zoonotic potential and varying pathogenicity for humans. Ebolaviruses are considered to circulate in wildlife with occasional spillover events into the human population which then often leads to severe disease outbreaks. Several studies indicate a significant role of bats as reservoir hosts in the ebolavirus ecology. However, pigs from the Philippines have been found to be naturally infected with Reston virus (RESTV), an ebolavirus that is thought to only cause asymptomatic infections in humans. The recent report of ebolavirus‐specific antibodies in pigs from Sierra Leone further supports natural infection of pigs with ebolaviruses. However, susceptibility of pigs to highly pathogenic Ebola virus (EBOV) was only shown under experimental settings and evidence for natural infection of pigs with EBOV is currently lacking. Between October and December 2017, we collected 308 serum samples from pigs in Guinea, West Africa, and tested for the presence of ebolavirus‐specific antibodies with different serological assays. Besides reactivity to EBOV nucleoproteins in ELISA and Western Blot for 19 (6.2%) and 13 (4.2%) samples respectively, four sera recognized Sudan virus (SUDV) NP in Western blot. Furthermore, four samples specifically detected EBOV or SUDV glycoprotein (GP) in an indirect immunofluorescence assay under native conditions. Virus neutralization assay based on EBOV (Mayinga isolate) revealed five weakly neutralizing sera. The finding of (cross‐) reactive and weakly neutralizing antibodies suggests the exposure of pigs from Guinea to ebolaviruses or ebola‐like viruses with their pathogenicity as well as their zoonotic potential remaining unknown. Future studies should investigate whether pigs can act as an amplifying host for ebolaviruses and whether there is a risk for spillover events.
    Keywords Text ; ddc:570 ; ELISA -- Ebola -- West Africa -- antibodies -- ebolaviruses -- neutralization test -- pigs -- serology
    Subject code 630
    Language English
    Publishing date 2019-10-18
    Publisher Wiley
    Publishing country de
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  6. Article ; Online: Randomized, Blinded, Dose-Ranging Trial of an Ebola Virus Glycoprotein Nanoparticle Vaccine With Matrix-M Adjuvant in Healthy Adults.

    Fries, Louis / Cho, Iksung / Krähling, Verena / Fehling, Sarah K / Strecker, Thomas / Becker, Stephan / Hooper, Jay W / Kwilas, Steven A / Agrawal, Sapeckshita / Wen, Judy / Lewis, Maggie / Fix, Amy / Thomas, Nigel / Flyer, David / Smith, Gale / Glenn, Gregory

    The Journal of infectious diseases

    2019  Volume 222, Issue 4, Page(s) 572–582

    Abstract: Background: Ebola virus (EBOV) epidemics pose a major public health risk. There currently is no licensed human vaccine against EBOV. The safety and immunogenicity of a recombinant EBOV glycoprotein (GP) nanoparticle vaccine formulated with or without ... ...

    Abstract Background: Ebola virus (EBOV) epidemics pose a major public health risk. There currently is no licensed human vaccine against EBOV. The safety and immunogenicity of a recombinant EBOV glycoprotein (GP) nanoparticle vaccine formulated with or without Matrix-M adjuvant were evaluated to support vaccine development.
    Methods: A phase 1, placebo-controlled, dose-escalation trial was conducted in 230 healthy adults to evaluate 4 EBOV GP antigen doses as single- or 2-dose regimens with or without adjuvant. Safety and immunogenicity were assessed through 1-year postdosing.
    Results: All EBOV GP vaccine formulations were well tolerated. Receipt of 2 doses of EBOV GP with adjuvant showed a rapid increase in anti-EBOV GP immunoglobulin G titers with peak titers observed on Day 35 representing 498- to 754-fold increases from baseline; no evidence of an antigen dose response was observed. Serum EBOV-neutralizing and binding antibodies using wild-type Zaire EBOV (ZEBOV) or pseudovirion assays were 3- to 9-fold higher among recipients of 2-dose EBOV GP with adjuvant, compared with placebo on Day 35, which persisted through 1 year.
    Conclusions: Ebola virus GP vaccine with Matrix-M adjuvant is well tolerated and elicits a robust and persistent immune response. These data suggest that further development of this candidate vaccine for prevention of EBOV disease is warranted.
    MeSH term(s) Adjuvants, Immunologic/administration & dosage ; Adult ; Antibodies, Neutralizing/blood ; Antibodies, Viral/blood ; Australia ; Ebola Vaccines/immunology ; Female ; Healthy Volunteers ; Hemorrhagic Fever, Ebola/prevention & control ; Humans ; Male ; Nanoparticles/administration & dosage ; Safety ; Saponins/administration & dosage ; Vaccination ; Viral Envelope Proteins/immunology ; Young Adult
    Chemical Substances Adjuvants, Immunologic ; Antibodies, Neutralizing ; Antibodies, Viral ; Ebola Vaccines ; Matrix-M ; Saponins ; Viral Envelope Proteins
    Language English
    Publishing date 2019-10-10
    Publishing country United States
    Document type Clinical Trial, Phase I ; Journal Article ; Multicenter Study ; Randomized Controlled Trial ; Research Support, Non-U.S. Gov't ; Research Support, U.S. Gov't, Non-P.H.S.
    ZDB-ID 3019-3
    ISSN 1537-6613 ; 0022-1899
    ISSN (online) 1537-6613
    ISSN 0022-1899
    DOI 10.1093/infdis/jiz518
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  7. Article ; Online: Comprehensive Characterization of Cellular Immune Responses Following Ebola Virus Infection.

    Dahlke, Christine / Lunemann, Sebastian / Kasonta, Rahel / Kreuels, Benno / Schmiedel, Stefan / Ly, My L / Fehling, Sarah K / Strecker, Thomas / Becker, Stephan / Altfeld, Marcus / Sow, Abdourahmane / Lohse, Ansgar W / Muñoz-Fontela, César / Addo, Marylyn M

    The Journal of infectious diseases

    2017  Volume 215, Issue 2, Page(s) 287–292

    Abstract: The West African Ebola virus disease (EVD) outbreak was the largest EVD outbreak in history. However, data on lymphocyte dynamics and the antigen specificity of T cells in Ebola survivors are scarce, and our understanding of EVD pathophysiology is ... ...

    Abstract The West African Ebola virus disease (EVD) outbreak was the largest EVD outbreak in history. However, data on lymphocyte dynamics and the antigen specificity of T cells in Ebola survivors are scarce, and our understanding of EVD pathophysiology is limited. A case of EVD survival in which the patient cleared Ebola virus (EBOV) infection without experimental drugs allowed for the detailed examination of lymphocyte dynamics. We demonstrate the persistence of T-cell activation well beyond viral clearance and detect EBOV-specific T cells. Our study provides significant insights into lymphocyte specificity during the recovery phase of EVD and may inform novel strategies to treat EVD.
    MeSH term(s) Ebolavirus/immunology ; Hemorrhagic Fever, Ebola/immunology ; Humans ; Immunity, Cellular ; Lymphocyte Activation ; T-Lymphocytes/immunology
    Language English
    Publishing date 2017-01-15
    Publishing country United States
    Document type Journal Article
    ZDB-ID 3019-3
    ISSN 1537-6613 ; 0022-1899
    ISSN (online) 1537-6613
    ISSN 0022-1899
    DOI 10.1093/infdis/jiw508
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  8. Article ; Online: Serological Evidence for the Circulation of Ebolaviruses in Pigs From Sierra Leone.

    Fischer, Kerstin / Jabaty, Juliet / Suluku, Roland / Strecker, Thomas / Groseth, Allison / Fehling, Sarah K / Balkema-Buschmann, Anne / Koroma, Bashiru / Schmidt, Kristina M / Atherstone, Christine / Weingartl, Hana M / Mettenleiter, Thomas C / Groschup, Martin H / Hoenen, Thomas / Diederich, Sandra

    The Journal of infectious diseases

    2018  Volume 218, Issue suppl_5, Page(s) S305–S311

    Abstract: Many human ebolavirus outbreaks have been linked to contact with wildlife including nonhuman primates and bats, which are assumed to serve as host species. However, it is largely unknown to what extent other animal species, particularly livestock, are ... ...

    Abstract Many human ebolavirus outbreaks have been linked to contact with wildlife including nonhuman primates and bats, which are assumed to serve as host species. However, it is largely unknown to what extent other animal species, particularly livestock, are involved in the transmission cycle or act as additional hosts for filoviruses. Pigs were identified as a susceptible host for Reston virus with subsequent transmission to humans reported in the Philippines. To date, there is no evidence of natural Ebola virus (EBOV) infection in pigs, although pigs were shown to be susceptible to EBOV infection under experimental settings. To investigate the potential role of pigs in the ecology of EBOV, we analyzed 400 porcine serum samples from Sierra Leone for the presence of ebolavirus-specific antibodies. Three samples reacted with ebolavirus nucleoproteins but had no neutralizing antibodies. Our results (1) suggest the circulation of ebolaviruses in swine in Sierra Leone that are antigenically related but not identical to EBOV and (2) could represent undiscovered ebolaviruses with unknown pathogenic and/or zoonotic potential.
    MeSH term(s) Animals ; Animals, Wild/blood ; Animals, Wild/immunology ; Animals, Wild/virology ; Antibodies, Neutralizing/blood ; Antibodies, Neutralizing/immunology ; Antibodies, Viral/blood ; Antibodies, Viral/immunology ; Ebolavirus/genetics ; Ebolavirus/immunology ; Female ; Hemorrhagic Fever, Ebola/blood ; Hemorrhagic Fever, Ebola/immunology ; Hemorrhagic Fever, Ebola/virology ; Humans ; Male ; Nucleoproteins/immunology ; Philippines ; Serum/immunology ; Serum/virology ; Sierra Leone ; Swine/virology
    Chemical Substances Antibodies, Neutralizing ; Antibodies, Viral ; Nucleoproteins
    Language English
    Publishing date 2018-07-05
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 3019-3
    ISSN 1537-6613 ; 0022-1899
    ISSN (online) 1537-6613
    ISSN 0022-1899
    DOI 10.1093/infdis/jiy330
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  9. Article ; Online: Acidic pH-Induced Conformations and LAMP1 Binding of the Lassa Virus Glycoprotein Spike.

    Li, Sai / Sun, Zhaoyang / Pryce, Rhys / Parsy, Marie-Laure / Fehling, Sarah K / Schlie, Katrin / Siebert, C Alistair / Garten, Wolfgang / Bowden, Thomas A / Strecker, Thomas / Huiskonen, Juha T

    PLoS pathogens

    2016  Volume 12, Issue 2, Page(s) e1005418

    Abstract: Lassa virus is an enveloped, bi-segmented RNA virus and the most prevalent and fatal of all Old World arenaviruses. Virus entry into the host cell is mediated by a tripartite surface spike complex, which is composed of two viral glycoprotein subunits, ... ...

    Abstract Lassa virus is an enveloped, bi-segmented RNA virus and the most prevalent and fatal of all Old World arenaviruses. Virus entry into the host cell is mediated by a tripartite surface spike complex, which is composed of two viral glycoprotein subunits, GP1 and GP2, and the stable signal peptide. Of these, GP1 binds to cellular receptors and GP2 catalyzes fusion between the viral envelope and the host cell membrane during endocytosis. The molecular structure of the spike and conformational rearrangements induced by low pH, prior to fusion, remain poorly understood. Here, we analyzed the three-dimensional ultrastructure of Lassa virus using electron cryotomography. Sub-tomogram averaging yielded a structure of the glycoprotein spike at 14-Å resolution. The spikes are trimeric, cover the virion envelope, and connect to the underlying matrix. Structural changes to the spike, following acidification, support a viral entry mechanism dependent on binding to the lysosome-resident receptor LAMP1 and further dissociation of the membrane-distal GP1 subunits.
    MeSH term(s) Animals ; Chlorocebus aethiops ; Glycoproteins/chemistry ; Glycoproteins/metabolism ; Hydrogen-Ion Concentration ; Lassa virus/chemistry ; Lassa virus/metabolism ; Lassa virus/ultrastructure ; Lysosomal Membrane Proteins/chemistry ; Lysosomal Membrane Proteins/metabolism ; Models, Molecular ; Molecular Conformation ; Multiprotein Complexes ; Protein Binding ; Protein Sorting Signals ; Protein Structure, Tertiary ; Vero Cells ; Viral Envelope Proteins/chemistry ; Viral Envelope Proteins/metabolism ; Virion ; Virus Internalization
    Chemical Substances Glycoproteins ; LAMP1 protein, human ; Lysosomal Membrane Proteins ; Multiprotein Complexes ; Protein Sorting Signals ; Viral Envelope Proteins
    Language English
    Publishing date 2016-02-05
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2205412-1
    ISSN 1553-7374 ; 1553-7374
    ISSN (online) 1553-7374
    ISSN 1553-7374
    DOI 10.1371/journal.ppat.1005418
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  10. Article ; Online: Detectable Vesicular Stomatitis Virus (VSV)-Specific Humoral and Cellular Immune Responses Following VSV-Ebola Virus Vaccination in Humans.

    Poetsch, Joseph H / Dahlke, Christine / Zinser, Madeleine E / Kasonta, Rahel / Lunemann, Sebastian / Rechtien, Anne / Ly, My L / Stubbe, Hans C / Krähling, Verena / Biedenkopf, Nadine / Eickmann, Markus / Fehling, Sarah K / Olearo, Flaminia / Strecker, Thomas / Sharma, Piyush / Lang, Karl S / Lohse, Ansgar W / Schmiedel, Stefan / Becker, Stephan /
    Addo, Marylyn M

    The Journal of infectious diseases

    2018  Volume 219, Issue 4, Page(s) 556–561

    Abstract: In response to the Ebola virus (EBOV) crisis of 2013-2016, a recombinant vesicular stomatitis virus (VSV)-based EBOV vaccine was clinically tested (NCT02283099). A single-dose regimen of VSV-EBOV revealed a safe and immunogenic profile and demonstrated ... ...

    Abstract In response to the Ebola virus (EBOV) crisis of 2013-2016, a recombinant vesicular stomatitis virus (VSV)-based EBOV vaccine was clinically tested (NCT02283099). A single-dose regimen of VSV-EBOV revealed a safe and immunogenic profile and demonstrated clinical efficacy. While EBOV-specific immune responses to this candidate vaccine have previously been investigated, limited human data on immunity to the VSV vector are available. Within the scope of a phase 1 study, we performed a comprehensive longitudinal analysis of adaptive immune responses to internal VSV proteins following VSV-EBOV immunization. While no preexisting immunity to the vector was observed, more than one-third of subjects developed VSV-specific cytotoxic T-lymphocyte responses and antibodies.
    MeSH term(s) Adult ; Antibody Formation ; Ebola Vaccines/administration & dosage ; Ebola Vaccines/immunology ; Humans ; Immunity, Cellular ; Longitudinal Studies ; Vaccines, Synthetic/administration & dosage ; Vaccines, Synthetic/immunology ; Vesiculovirus/immunology
    Chemical Substances Ebola Vaccines ; Vaccines, Synthetic
    Language English
    Publishing date 2018-11-19
    Publishing country United States
    Document type Clinical Trial, Phase I ; Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 3019-3
    ISSN 1537-6613 ; 0022-1899
    ISSN (online) 1537-6613
    ISSN 0022-1899
    DOI 10.1093/infdis/jiy565
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