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  1. Article ; Online: A subset of leukocyte immune-type receptors (LITRs) regulates phagocytosis in channel catfish (Ictalurus punctatus) leukocytes

    Crider, Jonathan / Wilson, Melanie / Felch, Kristianna L. / Dupre, Rebecca A. / Quiniou, Sylvie M.A. / Bengtén, Eva

    Molecular Immunology. 2023 Feb., v. 154 p.33-44

    2023  

    Abstract: Channel catfish, Ictalurus punctatus, leukocyte immune-type receptors (LITRs) constitute a large family of paired, immunoregulatory receptors unique to teleosts. A role for LITRs in phagocytosis has been proposed based on studies in mammalian cell lines; ...

    Abstract Channel catfish, Ictalurus punctatus, leukocyte immune-type receptors (LITRs) constitute a large family of paired, immunoregulatory receptors unique to teleosts. A role for LITRs in phagocytosis has been proposed based on studies in mammalian cell lines; however, LITR-mediated phagocytosis has not been examined in the catfish model. In this study, we use two anti-LITR monoclonal antibodies, CC41 and 125.2, to contrast the effects of crosslinking subsets of inhibitory and activating LITRs. Briefly, LITRs expressed by catfish γδ T cells, αβ T cells, and macrophage cell lines were crosslinked using mAb-conjugated fluorescent microbeads, and bead uptake was evaluated by flow cytometry and confirmed by confocal microscopy. A clear difference in the uptake of 125.2- and CC41-conjugated beads was observed. Crosslinking LITRs with mAb 125.2 resulted in efficient bead internalization, while mAb CC41 crosslinking of inhibitory LITRs resulted predominantly in a capturing phenotype. Pretreating catfish macrophages with mAb CC41 resulted in a marked decrease in LITR-mediated phagocytosis of 125.2-conjugated beads. Overall, these findings provide insight into fish immunobiology and validate LITRs as regulators of phagocytosis in catfish macrophages and γδ T cells.
    Keywords Ictalurus punctatus ; catfish ; confocal microscopy ; crosslinking ; flow cytometry ; fluorescence ; immunomodulation ; leukocytes ; macrophages ; mammals ; microbeads ; models ; phagocytosis ; phenotype ; APC ; μME ; BSA ; cfPBS ; cfRPMI ; CCV ; CEACAM ; Csk ; CTL ; CYT ; DAP10 ; DAP12 ; DICP ; FcR ; FcRL ; HA ; Ig ; IgSF ; ITAM ; ITIM ; ITSM ; KIR ; LAK ; LILR ; LITR ; MHC ; MLC ; nanoLC-MS/MS ; Nck ; NILT ; NITR ; NK ; NKG2D ; PBL ; PIGRL ; RBL ; TM ; TREM ; SHP-2 ; Siglec ; SIRP ; WGA ; Paired receptors ; Immunoregulatory receptors ; Inhibitory signaling ; Cytoplasmic tails ; Innate immunity ; Leukocyte immune-type receptors ; Channel catfish
    Language English
    Dates of publication 2023-02
    Size p. 33-44.
    Publishing place Elsevier Ltd
    Document type Article ; Online
    Note Use and reproduction
    ZDB-ID 424427-8
    ISSN 1872-9142 ; 0161-5890
    ISSN (online) 1872-9142
    ISSN 0161-5890
    DOI 10.1016/j.molimm.2022.12.009
    Database NAL-Catalogue (AGRICOLA)

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 166: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (1.OG)
    ab Jg. 2022: Lesesaal (EG)

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  2. Article ; Online: Interferons and interferon receptors in the channel catfish, Ictalurus punctatus

    Quiniou, Sylvie M.A. / Crider, Jonathan / Felch, Kristianna L. / Bengtén, Eva / Boudinot, Pierre

    Fish and Shellfish Immunology. 2022 Apr., v. 123 p.442-452

    2022  

    Abstract: In this work, we describe the complete repertoire of channel catfish, Ictalurus punctatus, IFNs and IFN receptor genes. Based on multiple genomic and transcriptomic resources we identified 16 type I IFN genes, which represent the six type I IFN subgroups ...

    Abstract In this work, we describe the complete repertoire of channel catfish, Ictalurus punctatus, IFNs and IFN receptor genes. Based on multiple genomic and transcriptomic resources we identified 16 type I IFN genes, which represent the six type I IFN subgroups previously defined in salmonids (a-f.) No representatives of subgroup h previously only found in percomorphs were identified. An expansion in copy numbers of subgroup d IFN genes was of particular interest, as this has not been reported in other fish species to date. Furthermore, we confirmed the presence of two type II ifn genes encoding orthologs of IFNγ and the teleost-specific IFNγRel. Six homologs of IFN type I receptor genes were found in an array that shows conserved synteny with human chromosome 21. Three homologs of type II IFN receptor genes were also identified. These type I and type II receptor sequences are compatible with the dual type I IFN receptors, and the potentially more complex type II IFN receptors described in teleosts. Our data provide a comprehensive resource for future studies of channel catfish innate antiviral immunity.
    Keywords Ictalurus punctatus ; Salmonidae ; chromosomes ; fish ; genomics ; humans ; immunity ; interferons ; shellfish ; transcriptomics ; Interferon ; Antiviral immunity ; Teleost ; Inflammation ; Innate immunity ; Channelcatfish
    Language English
    Dates of publication 2022-04
    Size p. 442-452.
    Publishing place Elsevier Ltd
    Document type Article ; Online
    ZDB-ID 1067738-0
    ISSN 1095-9947 ; 1050-4648
    ISSN (online) 1095-9947
    ISSN 1050-4648
    DOI 10.1016/j.fsi.2022.02.019
    Database NAL-Catalogue (AGRICOLA)

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    In stock of ZB MED Bonn / Germany

    Z 5917: Show issues

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  3. Article ; Online: A subset of leukocyte immune-type receptors (LITRs) regulates phagocytosis in channel catfish (Ictalurus punctatus) leukocytes.

    Crider, Jonathan / Wilson, Melanie / Felch, Kristianna L / Dupre, Rebecca A / Quiniou, Sylvie M A / Bengtén, Eva

    Molecular immunology

    2022  Volume 154, Page(s) 33–44

    Abstract: Channel catfish, Ictalurus punctatus, leukocyte immune-type receptors (LITRs) constitute a large family of paired, immunoregulatory receptors unique to teleosts. A role for LITRs in phagocytosis has been proposed based on studies in mammalian cell lines; ...

    Abstract Channel catfish, Ictalurus punctatus, leukocyte immune-type receptors (LITRs) constitute a large family of paired, immunoregulatory receptors unique to teleosts. A role for LITRs in phagocytosis has been proposed based on studies in mammalian cell lines; however, LITR-mediated phagocytosis has not been examined in the catfish model. In this study, we use two anti-LITR monoclonal antibodies, CC41 and 125.2, to contrast the effects of crosslinking subsets of inhibitory and activating LITRs. Briefly, LITRs expressed by catfish γδ T cells, αβ T cells, and macrophage cell lines were crosslinked using mAb-conjugated fluorescent microbeads, and bead uptake was evaluated by flow cytometry and confirmed by confocal microscopy. A clear difference in the uptake of 125.2- and CC41-conjugated beads was observed. Crosslinking LITRs with mAb 125.2 resulted in efficient bead internalization, while mAb CC41 crosslinking of inhibitory LITRs resulted predominantly in a capturing phenotype. Pretreating catfish macrophages with mAb CC41 resulted in a marked decrease in LITR-mediated phagocytosis of 125.2-conjugated beads. Overall, these findings provide insight into fish immunobiology and validate LITRs as regulators of phagocytosis in catfish macrophages and γδ T cells.
    MeSH term(s) Animals ; Ictaluridae/genetics ; Ictaluridae/metabolism ; Receptors, Immunologic ; Phagocytosis ; Leukocytes ; Catfishes ; Mammals
    Chemical Substances Receptors, Immunologic
    Language English
    Publishing date 2022-12-29
    Publishing country England
    Document type Journal Article ; Research Support, N.I.H., Extramural ; Research Support, U.S. Gov't, Non-P.H.S.
    ZDB-ID 424427-8
    ISSN 1872-9142 ; 0161-5890
    ISSN (online) 1872-9142
    ISSN 0161-5890
    DOI 10.1016/j.molimm.2022.12.009
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    Kategorien

    In stock of ZB MED Cologne/Königswinter

    Zs.A 166: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (1.OG)
    ab Jg. 2022: Lesesaal (EG)

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

  4. Article ; Online: Interferons and interferon receptors in the channel catfish, Ictalurus punctatus.

    Quiniou, Sylvie M A / Crider, Jonathan / Felch, Kristianna L / Bengtén, Eva / Boudinot, Pierre

    Fish & shellfish immunology

    2022  Volume 123, Page(s) 442–452

    Abstract: In this work, we describe the complete repertoire of channel catfish, Ictalurus punctatus, IFNs and IFN receptor genes. Based on multiple genomic and transcriptomic resources we identified 16 type I IFN genes, which represent the six type I IFN subgroups ...

    Abstract In this work, we describe the complete repertoire of channel catfish, Ictalurus punctatus, IFNs and IFN receptor genes. Based on multiple genomic and transcriptomic resources we identified 16 type I IFN genes, which represent the six type I IFN subgroups previously defined in salmonids (a-f.) No representatives of subgroup h previously only found in percomorphs were identified. An expansion in copy numbers of subgroup d IFN genes was of particular interest, as this has not been reported in other fish species to date. Furthermore, we confirmed the presence of two type II ifn genes encoding orthologs of IFNγ and the teleost-specific IFNγRel. Six homologs of IFN type I receptor genes were found in an array that shows conserved synteny with human chromosome 21. Three homologs of type II IFN receptor genes were also identified. These type I and type II receptor sequences are compatible with the dual type I IFN receptors, and the potentially more complex type II IFN receptors described in teleosts. Our data provide a comprehensive resource for future studies of channel catfish innate antiviral immunity.
    MeSH term(s) Animals ; Genome ; Ictaluridae/genetics ; Interferons/genetics ; Phylogeny ; Receptors, Interferon/genetics
    Chemical Substances Receptors, Interferon ; Interferons (9008-11-1)
    Language English
    Publishing date 2022-03-15
    Publishing country England
    Document type Journal Article
    ZDB-ID 1067738-0
    ISSN 1095-9947 ; 1050-4648
    ISSN (online) 1095-9947
    ISSN 1050-4648
    DOI 10.1016/j.fsi.2022.02.019
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

    In stock of ZB MED Bonn / Germany

    Z 5917: Show issues

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

    Inter-library loan at ZB MED

    Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

  5. Article ; Online: A Comprehensive Annotation of the Channel Catfish (

    Crider, Jonathan / Quiniou, Sylvie M A / Felch, Kristianna L / Showmaker, Kurt / Bengtén, Eva / Wilson, Melanie

    Frontiers in immunology

    2021  Volume 12, Page(s) 786402

    Abstract: The complete germline repertoires of the channel catfish, ...

    Abstract The complete germline repertoires of the channel catfish,
    MeSH term(s) Animals ; Evolution, Molecular ; Fish Proteins/genetics ; Fish Proteins/immunology ; Genes, T-Cell Receptor ; Genes, T-Cell Receptor alpha ; Genes, T-Cell Receptor beta ; Genes, T-Cell Receptor delta ; Genes, T-Cell Receptor gamma ; Genetic Loci ; Ictaluridae/genetics ; Ictaluridae/immunology ; Phylogeny ; Receptors, Antigen, T-Cell/genetics ; Receptors, Antigen, T-Cell/immunology ; Receptors, Antigen, T-Cell, alpha-beta/genetics ; Receptors, Antigen, T-Cell, alpha-beta/immunology ; Receptors, Antigen, T-Cell, gamma-delta/genetics ; Receptors, Antigen, T-Cell, gamma-delta/immunology ; Species Specificity ; Zebrafish/genetics ; Zebrafish/immunology ; Zebrafish Proteins/genetics ; Zebrafish Proteins/immunology
    Chemical Substances Fish Proteins ; Receptors, Antigen, T-Cell ; Receptors, Antigen, T-Cell, alpha-beta ; Receptors, Antigen, T-Cell, gamma-delta ; Zebrafish Proteins
    Language English
    Publishing date 2021-11-25
    Publishing country Switzerland
    Document type Comparative Study ; Journal Article ; Research Support, N.I.H., Extramural ; Research Support, U.S. Gov't, Non-P.H.S.
    ZDB-ID 2606827-8
    ISSN 1664-3224 ; 1664-3224
    ISSN (online) 1664-3224
    ISSN 1664-3224
    DOI 10.3389/fimmu.2021.786402
    Database MEDical Literature Analysis and Retrieval System OnLINE

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