Article ; Online: Venipuncture Site Influences Blood-drop Volume in C57BL/6 Mice.
Journal of the American Association for Laboratory Animal Science : JAALAS
2024
Abstract: Many experiments require the collection of serial blood samples from mice. However, the size of mice limits the volume of blood that can be safely collected as a survival procedure. In IACUC protocols, investigators may report the amount of blood they ... ...
Abstract | Many experiments require the collection of serial blood samples from mice. However, the size of mice limits the volume of blood that can be safely collected as a survival procedure. In IACUC protocols, investigators may report the amount of blood they collect from mice as a number of drops. Many institutions, including ours, use an anecdotal conversion factor (1 drop of mouse blood = 25 µL) to ensure that blood-collection volumes are compliant with institutional guidelines. To our knowledge, previous work has not experimentally determined the volume of a drop of mouse blood. In this 10-wk crossover experiment, 2 phlebotomists bled 30 C57BL/6J mice from 3 sites (facial, saphenous, and tail) using one or 2 different needle gauge sizes per site. Male and female mice were weighed weekly and divided among 5 groups ( |
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Language | English |
Publishing date | 2024-03-01 |
Publishing country | United States |
Document type | Journal Article |
ISSN | 2769-6677 |
ISSN (online) | 2769-6677 |
DOI | 10.30802/AALAS-JAALAS-23-000083 |
Database | MEDical Literature Analysis and Retrieval System OnLINE |
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