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  1. Book ; Online: Embryology

    Wu, Bin / Feng, Huai L

    Theory and Practice

    2019  

    Keywords Embryology ; proteomics, epigenetic, oocyte, in vitro fertilization, ion channels, bovine
    Language English
    Size 1 electronic resource (146 pages)
    Publisher IntechOpen
    Document type Book ; Online
    Note English
    HBZ-ID HT030647143
    ISBN 9781789856392 ; 1789856396
    Database ZB MED Catalogue: Medicine, Health, Nutrition, Environment, Agriculture

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  2. Article ; Online: Evaluation of an automated dish preparation system for IVF and embryo culture using a mouse mode.

    Zhu, Yan / Feng, Huai L / Jiang, Man-Xi

    Scientific reports

    2023  Volume 13, Issue 1, Page(s) 16490

    Abstract: Manual dish preparation for IVF in human fertility clinics or animal laboratories heavily relies on embryologists' experience, which can lead to occupational illness due to long-term and monotonous operation. Therefore, introducing an automated technique ...

    Abstract Manual dish preparation for IVF in human fertility clinics or animal laboratories heavily relies on embryologists' experience, which can lead to occupational illness due to long-term and monotonous operation. Therefore, introducing an automated technique to replace traditional methods is crucial for improving working efficiency and reducing work burden for embryologists. In the current study in the mouse, both manual and automated methods were used to prepare IVF or embryo culture dishes. A one-way analysis of variance was conducted to compare several factors, including preparation time, qualified rates, media osmolality of dishes, fertilization rates, and embryonic development to assess the efficiency and potential of automated preparation. The results showed that automation system significantly reduced the required time and increased the efficiencies and qualified rates of dish preparation, especially for embryo culture dishes, without significantly altering medium osmolalities. There were no significant differences between two preparations in fertilization rates and embryo development in mice. Thus, automated dish preparation can improve working efficiency and qualified rates while maintaining fertilization rates and subsequent embryonic development without compromising osmolality stability of medium. It presents a superior alternative to manual preparation, reducing the workload of embryologists and facilitating the standardization of operational procedures.
    MeSH term(s) Humans ; Pregnancy ; Animals ; Female ; Fertilization in Vitro/methods ; Embryo Culture Techniques/methods ; Embryonic Development ; Time-Lapse Imaging/methods ; Osmolar Concentration ; Culture Media
    Chemical Substances Culture Media
    Language English
    Publishing date 2023-10-01
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2615211-3
    ISSN 2045-2322 ; 2045-2322
    ISSN (online) 2045-2322
    ISSN 2045-2322
    DOI 10.1038/s41598-023-43665-y
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article: Automation in vitrification and thawing of mouse oocytes and embryos.

    Zhu, Yan / Zhang, Quan-Jun / Feng, Huai L / Luo, Jin / Miao, Shu / Jiang, Man-Xi

    Frontiers in cell and developmental biology

    2023  Volume 11, Page(s) 1330684

    Abstract: Vitrification is a common technique for cryopreserving oocytes or embryos. However, manual vitrification is tedious and labor-intensive, and can be subject to variations caused by human factors. To address these challenges, we developed an automated ... ...

    Abstract Vitrification is a common technique for cryopreserving oocytes or embryos. However, manual vitrification is tedious and labor-intensive, and can be subject to variations caused by human factors. To address these challenges, we developed an automated vitrification-thawing system (AVTS) based on a cryo-handle. Our study firstly assessed the efficiency of cryoprotectant exchange through comparing the osmolalities of fresh and collected solutions during automated vitrification and thawing, and evaluated the cooling and warming rates of the cryo-handle. We also compared mouse oocyte survival, fertilization and embryo development after thawing and ICSI, and the development of re-frozen cleavage embryos between manual operation and automated system. The results showed that the osmolalities of collected samples were within normal range and comparable to fresh solutions. Furthermore, the automated system could obtain the reliable cooling and warming rates. Particularly, there were no significant differences in oocyte survival rates, fertilization rates, and subsequent embryo development and its quality between two procedures. Our findings suggest that AVTS has no impact on osmolalities of vitrification and thawing solutions, ensuring the proper exchange of cryoprotectants. The cryo-handle also shows the ability to achieve reliable cooling and warming rates, which benefits for the cryopreservation and thawing process. Moreover, the results from mouse oocytes and embryos indicate that automated system has effectively maintained the survival and fertilization of frozen oocytes and supported subsequent embryo development. Therefore, the automated vitrification and thawing system will inevitably represent a superior alternative to manual operation.
    Language English
    Publishing date 2023-12-21
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2737824-X
    ISSN 2296-634X
    ISSN 2296-634X
    DOI 10.3389/fcell.2023.1330684
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article ; Online: Oocyte cryopreservation in women after childhood and adolescent cancer.

    Li, Huiying / Xiang, Wenpei / Feng, Huai L

    Fertility and sterility

    2021  Volume 116, Issue 4, Page(s) 1096–1097

    MeSH term(s) Adolescent ; Child ; Cryopreservation ; Female ; Humans ; Neoplasms/epidemiology ; Oocytes
    Language English
    Publishing date 2021-09-04
    Publishing country United States
    Document type Editorial ; Comment
    ZDB-ID 80133-1
    ISSN 1556-5653 ; 0015-0282
    ISSN (online) 1556-5653
    ISSN 0015-0282
    DOI 10.1016/j.fertnstert.2021.08.003
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 73: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (1.OG)
    ab Jg. 2022: Lesesaal (EG)

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  5. Article: Editorial: Ovarian aging and reproduction.

    Liu, Mengyu / Zhang, Dan / Li, Wenzhu / Xu, Bufang / Feng, Huai L

    Frontiers in endocrinology

    2022  Volume 13, Page(s) 1081348

    MeSH term(s) Female ; Humans ; Ovary ; Reproduction
    Language English
    Publishing date 2022-11-28
    Publishing country Switzerland
    Document type Editorial ; Research Support, Non-U.S. Gov't
    ZDB-ID 2592084-4
    ISSN 1664-2392
    ISSN 1664-2392
    DOI 10.3389/fendo.2022.1081348
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  6. Article ; Online: Ovulation, fertilization and preimplantation embryonic development in raccoon dogs (Nyctereutes procyonoides).

    Xu, Baozeng / Feng, Huai L

    Animal reproduction science

    2017  Volume 176, Page(s) 78–84

    Abstract: A study involving 32 sexual mature females was conducted to characterize ovulation, fertilization and early embryonic development in vivo in raccoon dogs. Oocytes and embryos were collected from the oviducts and uteri, evaluated by stereomicroscopy. ... ...

    Abstract A study involving 32 sexual mature females was conducted to characterize ovulation, fertilization and early embryonic development in vivo in raccoon dogs. Oocytes and embryos were collected from the oviducts and uteri, evaluated by stereomicroscopy. Ovulation occurred 25-32h after a female first accepted mounting, regardless of copulation, when the females were paired with a male in the same cage. Ovulated oocytes were at the primary stage. The number of ovulated eggs in females with or without mating was 9.96±2.65 and 9.00±1.92, respectively. Embryos at 2-4 cell, 8-16 cell, morula, blastocyst, and hatched blastocyst stage were observed at 29-73, 48-100, 98-126, 169-198 and 217-268h after first mating, respectively. Embryos were located in the oviduct prior to 4-cell stage and moved into the uterus after 16-cell stage. Embryos at different stages were often obtained from the same female. During the zygote underwent a series of cleavage divisions, the diameter of the embryo cell mass continuously increased through the 2-cell and 4-cell stage, then started to decrease and was the minimum size at the morula stage. At the blastocyst stage, embryos increased in volume, and finally developed into a hatching blastocyst with a thinner zona pellucida. This is the first full report of preimplantation embryonic development in the raccoon dog, which will facilitate the application of advanced assisted reproductive technology in canine species.
    MeSH term(s) Animals ; Blastula/growth & development ; Female ; Fertilization/physiology ; Male ; Ovulation/physiology ; Raccoon Dogs/embryology
    Language English
    Publishing date 2017-01
    Publishing country Netherlands
    Document type Journal Article
    ZDB-ID 429674-6
    ISSN 1873-2232 ; 0378-4320
    ISSN (online) 1873-2232
    ISSN 0378-4320
    DOI 10.1016/j.anireprosci.2016.12.001
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Bonn / Germany

    Z 3232: Show issues
    Z 80/707: Show issues

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  7. Article ; Online: Biological roles of l-carnitine in oocyte and early embryo development.

    Li, Jiajian / Liu, Lingyan / Weng, Jing / Yin, Tai-Lang / Yang, Jing / Feng, Huai L

    Molecular reproduction and development

    2021  Volume 88, Issue 10, Page(s) 673–685

    Abstract: Poor oocyte quality is responsible for female infertility. Multiple studies have been carried out to find supplements to enhance oocyte quality and mitigate infertility problems. l-carnitine and its derivatives have diverse roles in developing oocytes ... ...

    Abstract Poor oocyte quality is responsible for female infertility. Multiple studies have been carried out to find supplements to enhance oocyte quality and mitigate infertility problems. l-carnitine and its derivatives have diverse roles in developing oocytes and early embryos. This review focuses on the in vitro and in vivo studies that using l-carnitine alone or in combination with other supplements for oocyte quality enhancement. The key roles of l-carnitine in oocyte quality and embryo growth were summarized, and the underlying mechanism was also elucidated. l-carnitine helps in the lipid metabolism process by controlling the transfer of fatty acids to mitochondria for β-oxidation. l-carnitine modulates glucose metabolism and enhances respiratory chain enzyme activity. Furthermore, it acts as an antioxidant to prevent oxidative damage and inhibit apoptosis, a signal in response to oxidative stress. Results show the potential of l-carnitine as a potential agent in assisted reproductive technology to improve oocyte quality and the subsequent embryonic development.
    MeSH term(s) Antioxidants/metabolism ; Carnitine/metabolism ; Carnitine/pharmacology ; Embryonic Development ; Female ; Humans ; In Vitro Oocyte Maturation Techniques ; Oocytes/metabolism ; Pregnancy
    Chemical Substances Antioxidants ; Carnitine (S7UI8SM58A)
    Language English
    Publishing date 2021-10-07
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't ; Review
    ZDB-ID 20321-x
    ISSN 1098-2795 ; 1040-452X
    ISSN (online) 1098-2795
    ISSN 1040-452X
    DOI 10.1002/mrd.23542
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 2759: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (2.OG)
    ab Jg. 2022: Lesesaal (EG)

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  8. Article: Assisted reproductive technology in China: compliance and non-compliance.

    Qiao, Jie / Feng, Huai L

    Translational pediatrics

    2015  Volume 3, Issue 2, Page(s) 91–97

    Abstract: According to the WHO, infertility and sterility will be the third-most serious disease worldwide in the 21st century, after cancer and cardiovascular diseases. In contrast to developed countries, assisted reproductive technology (ART) were not offered in ...

    Abstract According to the WHO, infertility and sterility will be the third-most serious disease worldwide in the 21st century, after cancer and cardiovascular diseases. In contrast to developed countries, assisted reproductive technology (ART) were not offered in China until the mid-1980s with the first in vitro fertilization (IVF) infant born in Taiwan in 1985, then Hong Kong in 1986, and mainland China in 1988, respectively. Since those inceptions, the practice of ART in China has evoked a variety of social, cultural, political and one-child policy responses that have resulted in restrictions on the number of IVF cycles performed annually. According to recent survey, an estimate 40-50 million women and 45 million men suffered from infertility, which is estimated that more than ten million Chinese infertile couples require ART treatment. However, it has limited access to ART facilities, many of them may not have a child are whirling to all types of fertility therapies. Exposure to radiation, pesticides and other environmental pollutants, work-related stress and unhealthy lifestyles are believed to contribute to the increasing incidence of infertility in China. The aim of this first report is to provide China nationwide ART data and government policy in compliance and 
non-compliance, particularly related to family plan policy in China.
    Language English
    Publishing date 2015-08-28
    Publishing country China
    Document type Journal Article ; Review
    ZDB-ID 2901309-4
    ISSN 2224-4344 ; 2224-4344 ; 2224-4336
    ISSN (online) 2224-4344
    ISSN 2224-4344 ; 2224-4336
    DOI 10.3978/j.issn.2224-4336.2014.01.06
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  9. Article: Comparison of different stimulation protocols used in in vitro fertilization: a review.

    Shrestha, Deekshya / La, Xiaolin / Feng, Huai L

    Annals of translational medicine

    2015  Volume 3, Issue 10, Page(s) 137

    Abstract: Infertility is one of the major medical problems in the western world caused by genetic or epigenetic factors, or both, which has led to continuous research and advancements in the field of assisted reproductive technology (ART). Many stimulation ... ...

    Abstract Infertility is one of the major medical problems in the western world caused by genetic or epigenetic factors, or both, which has led to continuous research and advancements in the field of assisted reproductive technology (ART). Many stimulation protocols are available for controlled ovarian hyperstimulation (COH) in in vitro fertilization (IVF). This review compares the agonist long protocol, antagonist protocol and minimal stimulation protocol. Gonadotropin-releasing hormone (GnRH) antagonist and minimal stimulation protocol has shorter duration of treatment and less gonadotropin use. GnRH agonist long protocol is better in folliculogenesis and pregnancy rate, which is the imperative goal of COH. Despite its costly and lengthy approach, GnRH agonist long protocol has delivered satisfactory results in most women. On the other hand, patients with poor ovarian reserve may have greater advantage when considering minimal stimulation protocol. Evidently, it is crucial to have a larger scale studies with more focused comparisons, which take into account the differences in patients' response criteria and additional confounding variables (age, BMI, previous IVF outcomes etc.), in order to reach to a more definite conclusions.
    Language English
    Publishing date 2015-04-22
    Publishing country China
    Document type Journal Article ; Review
    ZDB-ID 2893931-1
    ISSN 2305-5847 ; 2305-5839
    ISSN (online) 2305-5847
    ISSN 2305-5839
    DOI 10.3978/j.issn.2305-5839.2015.04.09
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  10. Article ; Online: Extra- and intra-ovarian factors in polycystic ovary syndrome: impact on oocyte maturation and embryo developmental competence.

    Qiao, Jie / Feng, Huai L

    Human reproduction update

    2010  Volume 17, Issue 1, Page(s) 17–33

    Abstract: Background: Polycystic ovary syndrome (PCOS) is a common metabolic dysfunction and heterogeneous endocrine disorder in women of reproductive age. Although patients with PCOS are typically characterized by increased numbers of oocytes retrieved during ... ...

    Abstract Background: Polycystic ovary syndrome (PCOS) is a common metabolic dysfunction and heterogeneous endocrine disorder in women of reproductive age. Although patients with PCOS are typically characterized by increased numbers of oocytes retrieved during IVF, they are often of poor quality, leading to lower fertilization, cleavage and implantation rates, and a higher miscarriage rate.
    Methods: For this review, we searched the database MEDLINE (1950 to January 2010) and Google for all full texts and/or abstract articles published in English with content related to oocyte maturation and embryo developmental competence.
    Results: The search showed that alteration of many factors may directly or indirectly impair the competence of maturating oocytes through endocrine and local paracrine/autocrine actions, resulting in a lower pregnancy rate in patients with PCOS. The extra-ovarian factors identified included gonadotrophins, hyperandrogenemia and hyperinsulinemia, although intra-ovarian factors included members of the epidermal, fibroblast, insulin-like and neurotrophin families of growth factors, as well as the cytokines.
    Conclusions: Any abnormality in the extra- and/or intra-ovarian factors may negatively affect the granulosa cell-oocyte interaction, oocyte maturation and potential embryonic developmental competence, contributing to unsuccessful outcomes for patients with PCOS who are undergoing assisted reproduction.
    MeSH term(s) Embryonic Development ; Female ; Follicle Stimulating Hormone/deficiency ; Humans ; Hyperandrogenism/complications ; Hyperinsulinism/complications ; Intercellular Signaling Peptides and Proteins/physiology ; Luteinizing Hormone/metabolism ; Oocytes/growth & development ; Oocytes/metabolism ; Ovary/physiopathology ; Paracrine Communication ; Polycystic Ovary Syndrome/complications ; Polycystic Ovary Syndrome/embryology ; Polycystic Ovary Syndrome/metabolism ; Pregnancy
    Chemical Substances Intercellular Signaling Peptides and Proteins ; Luteinizing Hormone (9002-67-9) ; Follicle Stimulating Hormone (9002-68-0)
    Language English
    Publishing date 2010-07-16
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't ; Review
    ZDB-ID 1286738-x
    ISSN 1460-2369 ; 1355-4786
    ISSN (online) 1460-2369
    ISSN 1355-4786
    DOI 10.1093/humupd/dmq032
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 4378: Show issues Location:
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