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  1. Article ; Online: Myonecrosis Induction by Intramuscular Injection of CTX.

    Feno, Simona / Munari, Fabio / Gherardi, Gaia / Reane, Denis Vecellio / D'Angelo, Donato / Viola, Antonella / Rizzuto, Rosario / Raffaello, Anna

    Bio-protocol

    2023  Volume 13, Issue 1, Page(s) e4587

    Abstract: Skeletal muscle, one of the most abundant tissue in the body, is a highly regenerative tissue. Indeed, compared to other tissues that are not able to regenerate after injury, skeletal muscle can fully regenerate upon mechanically, chemically, and ... ...

    Abstract Skeletal muscle, one of the most abundant tissue in the body, is a highly regenerative tissue. Indeed, compared to other tissues that are not able to regenerate after injury, skeletal muscle can fully regenerate upon mechanically, chemically, and infection-induced trauma. Several injury models have been developed to thoroughly investigate the physiological mechanisms regulating skeletal muscle regeneration. This protocol describes how to induce muscle regeneration by taking advantage of a cardiotoxin (CTX)-induced muscle injury model. The overall steps include CTX injection of tibialis anterior (TA) muscles of BL6N mice, collection of regenerating muscles at different time points after CTX injury, and histological characterization of regenerating muscles. Our protocol, compared with others such as those for freeze-induced injury models, avoids laceration or infections of the muscles since it involves neither surgery nor suture. In addition, our protocol is highly reproducible, since it causes homogenous myonecrosis of the whole muscle, and further reduces animal pain and stress. Graphical abstract.
    Language English
    Publishing date 2023-01-05
    Publishing country United States
    Document type Journal Article
    ZDB-ID 2833269-6
    ISSN 2331-8325 ; 2331-8325
    ISSN (online) 2331-8325
    ISSN 2331-8325
    DOI 10.21769/BioProtoc.4587
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article: T Cell Features in Glioblastoma May Guide Therapeutic Strategies to Overcome Microenvironment Immunosuppression.

    Losurdo, Agnese / Di Muzio, Antonio / Cianciotti, Beatrice Claudia / Dipasquale, Angelo / Persico, Pasquale / Barigazzi, Chiara / Bono, Beatrice / Feno, Simona / Pessina, Federico / Santoro, Armando / Simonelli, Matteo

    Cancers

    2024  Volume 16, Issue 3

    Abstract: Glioblastoma (GBM) is the most aggressive and lethal primary brain tumor, bearing a survival estimate below 10% at five years, despite standard chemoradiation treatment. At recurrence, systemic treatment options are limited and the standard of care is ... ...

    Abstract Glioblastoma (GBM) is the most aggressive and lethal primary brain tumor, bearing a survival estimate below 10% at five years, despite standard chemoradiation treatment. At recurrence, systemic treatment options are limited and the standard of care is not well defined, with inclusion in clinical trials being highly encouraged. So far, the use of immunotherapeutic strategies in GBM has not proved to significantly improve patients' prognosis in the treatment of newly diagnosed GBM, nor in the recurrent setting. Probably this has to do with the unique immune environment of the central nervous system, which harbors several immunosuppressive/pro-tumorigenic factors, both soluble (e.g., TGF-β, IL-10, STAT3, prostaglandin E2, and VEGF) and cellular (e.g., Tregs, M2 phenotype TAMs, and MDSC). Here we review the immune composition of the GBMs microenvironment, specifically focusing on the phenotype and function of the T cell compartment. Moreover, we give hints on the therapeutic strategies, such as immune checkpoint blockade, vaccinations, and adoptive cell therapy, that, interacting with tumor-infiltrating lymphocytes, might both target in different ways the tumor microenvironment and potentiate the activity of standard therapies. The path to be followed in advancing clinical research on immunotherapy for GBM treatment relies on a twofold strategy: testing combinatorial treatments, aiming to restore active immune anti-tumor responses, tackling immunosuppression, and additionally, designing more phase 0 and window opportunity trials with solid translational analyses to gain deeper insight into the on-treatment shaping of the GBM microenvironment.
    Language English
    Publishing date 2024-01-31
    Publishing country Switzerland
    Document type Journal Article ; Review
    ZDB-ID 2527080-1
    ISSN 2072-6694
    ISSN 2072-6694
    DOI 10.3390/cancers16030603
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article ; Online: The molecular complexity of the Mitochondrial Calcium Uniporter.

    Feno, Simona / Rizzuto, Rosario / Raffaello, Anna / Vecellio Reane, Denis

    Cell calcium

    2020  Volume 93, Page(s) 102322

    Abstract: The role of mitochondria in regulating cellular ... ...

    Abstract The role of mitochondria in regulating cellular Ca
    MeSH term(s) Animals ; Calcium Channels/chemistry ; Calcium Channels/metabolism ; Humans ; Models, Biological ; Organ Specificity ; Protein Subunits/metabolism ; Structure-Activity Relationship
    Chemical Substances Calcium Channels ; Protein Subunits ; mitochondrial calcium uniporter
    Language English
    Publishing date 2020-11-22
    Publishing country Netherlands
    Document type Journal Article ; Research Support, Non-U.S. Gov't ; Review
    ZDB-ID 757687-0
    ISSN 1532-1991 ; 0143-4160
    ISSN (online) 1532-1991
    ISSN 0143-4160
    DOI 10.1016/j.ceca.2020.102322
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 1596: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (1.OG)
    ab Jg. 2022: Lesesaal (EG)

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  4. Article ; Online: Crosstalk between Calcium and ROS in Pathophysiological Conditions.

    Feno, Simona / Butera, Gaia / Vecellio Reane, Denis / Rizzuto, Rosario / Raffaello, Anna

    Oxidative medicine and cellular longevity

    2019  Volume 2019, Page(s) 9324018

    Abstract: Calcium ions are highly versatile intracellular signals that regulate many cellular processes. The key to achieving this pleiotropic role is the spatiotemporal control of calcium concentration evoked by an extensive molecular repertoire of signalling ... ...

    Abstract Calcium ions are highly versatile intracellular signals that regulate many cellular processes. The key to achieving this pleiotropic role is the spatiotemporal control of calcium concentration evoked by an extensive molecular repertoire of signalling components. Among these, reactive oxygen species (ROS) signalling, together with calcium signalling, plays a crucial role in controlling several physiopathological events. Although initially considered detrimental by-products of aerobic metabolism, it is now widely accepted that ROS, in subtoxic levels, act as signalling molecules. However, dysfunctions in the mechanisms controlling the physiological ROS concentration affect cellular homeostasis, leading to the pathogenesis of various disorders.
    MeSH term(s) Calcium/metabolism ; Cell Physiological Phenomena/physiology ; Humans ; Reactive Oxygen Species/metabolism
    Chemical Substances Reactive Oxygen Species ; Calcium (SY7Q814VUP)
    Language English
    Publishing date 2019-04-24
    Publishing country United States
    Document type Journal Article ; Review
    ISSN 1942-0994
    ISSN (online) 1942-0994
    DOI 10.1155/2019/9324018
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article ; Online: High-Throughput Screening Using Photoluminescence Probe to Measure Intracellular Calcium Levels.

    Feno, Simona / Di Marco, Giulia / De Mario, Agnese / Monticelli, Halenya / Reane, Denis Vecellio

    Methods in molecular biology (Clifton, N.J.)

    2019  Volume 1925, Page(s) 1–14

    Abstract: Aequorin, a 22 kDa protein produced by the jellyfish Aequorea victoria, was the first probe used to measure ... ...

    Abstract Aequorin, a 22 kDa protein produced by the jellyfish Aequorea victoria, was the first probe used to measure Ca
    MeSH term(s) Aequorin/chemistry ; Aequorin/metabolism ; Animals ; Calcium/analysis ; Calcium/metabolism ; Cytosol/metabolism ; Endoplasmic Reticulum/metabolism ; HeLa Cells ; High-Throughput Screening Assays/methods ; Humans ; Imidazoles/chemistry ; Imidazoles/metabolism ; Luminescent Agents/chemistry ; Luminescent Agents/metabolism ; Luminescent Measurements/methods ; Mitochondria/metabolism ; Models, Molecular ; Pyrazines/chemistry ; Pyrazines/metabolism ; Scyphozoa/chemistry
    Chemical Substances Imidazoles ; Luminescent Agents ; Pyrazines ; coelenterazine (3O1CB88RRD) ; Aequorin (50934-79-7) ; Calcium (SY7Q814VUP)
    Language English
    Publishing date 2019-01-23
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ISSN 1940-6029
    ISSN (online) 1940-6029
    DOI 10.1007/978-1-4939-9018-4_1
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  6. Article ; Online: The dominant-negative mitochondrial calcium uniporter subunit MCUb drives macrophage polarization during skeletal muscle regeneration.

    Feno, Simona / Munari, Fabio / Reane, Denis Vecellio / Gissi, Rosanna / Hoang, Dieu-Huong / Castegna, Alessandra / Chazaud, Bénédicte / Viola, Antonella / Rizzuto, Rosario / Raffaello, Anna

    Science signaling

    2021  Volume 14, Issue 707, Page(s) eabf3838

    Abstract: Damaged skeletal muscle can regenerate because of the coordinated action of immune cells with muscle stem cells, called satellite cells. Proinflammatory macrophages infiltrate skeletal muscle soon after injury to sustain the proliferation of satellite ... ...

    Abstract Damaged skeletal muscle can regenerate because of the coordinated action of immune cells with muscle stem cells, called satellite cells. Proinflammatory macrophages infiltrate skeletal muscle soon after injury to sustain the proliferation of satellite cells. These macrophages later acquire the anti-inflammatory phenotype and promote the differentiation and fusion of satellite cells. Here, we showed that MCUb, the dominant-negative subunit of the mitochondrial calcium uniporter (MCU) complex, promotes muscle regeneration by controlling macrophage responses. Macrophages lacking MCUb lost the ability to efficiently acquire the anti-inflammatory profile, and mice with MCUb-deficient macrophages showed delayed regeneration through exhaustion of the satellite cell pool. MCUb ablation altered macrophage metabolism by promoting glycolysis and the accumulation of TCA cycle intermediates, which was accompanied by the stabilization of HIF-1α, the master transcriptional regulator of the macrophage proinflammatory program. Together, these data demonstrate that MCUb abundance is tightly controlled in macrophages to enable satellite cell functional differentiation and recovery of tissue homeostasis after damage.
    MeSH term(s) Calcium/metabolism ; Calcium Channels ; Macrophages/metabolism ; Muscle, Skeletal/metabolism
    Chemical Substances Calcium Channels ; mitochondrial calcium uniporter ; Calcium (SY7Q814VUP)
    Language English
    Publishing date 2021-11-02
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2417226-1
    ISSN 1937-9145 ; 1945-0877
    ISSN (online) 1937-9145
    ISSN 1945-0877
    DOI 10.1126/scisignal.abf3838
    Database MEDical Literature Analysis and Retrieval System OnLINE

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