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  1. Book ; Online ; Thesis: Single-molecule dynamics at a bottleneck: a systematic study of the narrow escape problem in a disc

    Meiser, Elisabeth [Verfasser] / Fenz, Susanne [Gutachter] / Sauer, Markus [Gutachter]

    2023  

    Author's details Elisabeth Meiser ; Gutachter: Susanne Fenz, Markus Sauer
    Keywords Biowissenschaften, Biologie ; Life Science, Biology
    Subject code sg570
    Language English
    Publisher Universität Würzburg
    Publishing place Würzburg
    Document type Book ; Online ; Thesis
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  2. Article ; Online: Measuring the Invisible: Determining the Size of Growing Nanodomains Using the "Inverse FCS".

    Fenz, Susanne F / Smith, Ana-Sunčana / Monzel, Cornelia

    Biophysical journal

    2017  Volume 112, Issue 11, Page(s) 2245–2246

    MeSH term(s) Cell Membrane ; Lipids
    Chemical Substances Lipids
    Language English
    Publishing date 2017-05-24
    Publishing country United States
    Document type Journal Article ; Comment
    ZDB-ID 218078-9
    ISSN 1542-0086 ; 0006-3495
    ISSN (online) 1542-0086
    ISSN 0006-3495
    DOI 10.1016/j.bpj.2017.04.014
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 235: Show issues Location:
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  3. Book ; Online ; Thesis: Single-molecule fluorescence microscopy in live Trypanosomabrucei and model membranes

    Glogger, Marius [Verfasser] / Fenz, Susanne [Gutachter] / Sauer, Markus [Gutachter]

    2018  

    Author's details Marius Glogger ; Gutachter: Susanne Fenz, Markus Sauer
    Keywords Tiere (Zoologie) ; Animals (Zoology)
    Subject code sg590
    Language English
    Publisher Universität Würzburg
    Publishing place Würzburg
    Document type Book ; Online ; Thesis
    Database Digital theses on the web

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  4. Article ; Online: Giant vesicles as cell models.

    Fenz, Susanne F / Sengupta, Kheya

    Integrative biology : quantitative biosciences from nano to macro

    2012  Volume 4, Issue 9, Page(s) 982–995

    Abstract: Tremendous progress has been made in recent years in understanding the working of the living cell, including its micro-anatomy, signalling networks, and regulation of genes. However, an understanding of cellular phenomena using fundamental laws starting ... ...

    Abstract Tremendous progress has been made in recent years in understanding the working of the living cell, including its micro-anatomy, signalling networks, and regulation of genes. However, an understanding of cellular phenomena using fundamental laws starting from first principles is still very far away. Part of the reason is that a cell is an active and exquisitely complex system where every part is linked to the other. Thus, it is difficult or even impossible to design experiments that selectively and exclusively probe a chosen aspect of the cell. Various kinds of idealised systems and cell models have been used to circumvent this problem. An important example is a giant unilamellar vesicle (GUV, also called giant liposome), which provides a cell-sized confined volume to study biochemical reactions as well as self-assembly processes that occur on the membrane. The GUV membrane can be designed suitably to present selected, correctly-oriented cell-membrane proteins, whose mobility is confined to two dimensions. Here, we present recent advances in GUV design and the use of GUVs as cell models that enable quantitative testing leading to insight into the working of real cells. We briefly recapitulate important classical concepts in membrane biophysics emphasising the advantages and limitations of GUVs. We then present results obtained over the last decades using GUVs, choosing the formation of membrane domains and cell adhesion as examples for in-depth treatment. Insight into cell adhesion obtained using micro-interferometry is treated in detail. We conclude by summarising the open questions and possible future directions.
    MeSH term(s) Cell Adhesion/physiology ; Cell Membrane/physiology ; Cell Physiological Phenomena ; Lipid Bilayers/chemistry ; Membrane Proteins/physiology ; Unilamellar Liposomes/chemistry
    Chemical Substances Lipid Bilayers ; Membrane Proteins ; Unilamellar Liposomes
    Language English
    Publishing date 2012-09
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't ; Review
    ZDB-ID 2480063-6
    ISSN 1757-9708 ; 1757-9694
    ISSN (online) 1757-9708
    ISSN 1757-9694
    DOI 10.1039/c2ib00188h
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article: Giant vesicles as cell models

    Fenz, Susanne F / Sengupta, Kheya

    Integrative biology. 2012 Aug. 21, v. 4, no. 9

    2012  

    Abstract: Tremendous progress has been made in recent years in understanding the working of the living cell, including its micro-anatomy, signalling networks, and regulation of genes. However, an understanding of cellular phenomena using fundamental laws starting ... ...

    Abstract Tremendous progress has been made in recent years in understanding the working of the living cell, including its micro-anatomy, signalling networks, and regulation of genes. However, an understanding of cellular phenomena using fundamental laws starting from first principles is still very far away. Part of the reason is that a cell is an active and exquisitely complex system where every part is linked to the other. Thus, it is difficult or even impossible to design experiments that selectively and exclusively probe a chosen aspect of the cell. Various kinds of idealised systems and cell models have been used to circumvent this problem. An important example is a giant unilamellar vesicle (GUV, also called giant liposome), which provides a cell-sized confined volume to study biochemical reactions as well as self-assembly processes that occur on the membrane. The GUV membrane can be designed suitably to present selected, correctly-oriented cell-membrane proteins, whose mobility is confined to two dimensions. Here, we present recent advances in GUV design and the use of GUVs as cell models that enable quantitative testing leading to insight into the working of real cells. We briefly recapitulate important classical concepts in membrane biophysics emphasising the advantages and limitations of GUVs. We then present results obtained over the last decades using GUVs, choosing the formation of membrane domains and cell adhesion as examples for in-depth treatment. Insight into cell adhesion obtained using micro-interferometry is treated in detail. We conclude by summarising the open questions and possible future directions.
    Keywords biophysics ; cell adhesion ; chemical reactions ; genes ; membrane proteins ; models
    Language English
    Dates of publication 2012-0821
    Size p. 982-995.
    Publishing place The Royal Society of Chemistry
    Document type Article
    ZDB-ID 2480063-6
    ISSN 1757-9708 ; 1757-9694
    ISSN (online) 1757-9708
    ISSN 1757-9694
    DOI 10.1039/c2ib00188h
    Database NAL-Catalogue (AGRICOLA)

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  6. Book ; Thesis: Cell-cell adhesion mediated by mobile receptor-ligand pairs

    Fenz, Susanne Franziska

    a biomimetic study

    2008  

    Author's details vorgelegt von Susanne Franziska Fenz
    Language English
    Size V, 200 S., Ill., graph. Darst.
    Document type Book ; Thesis
    Thesis / German Habilitation thesis Univ., Diss.--Bonn, 2008
    Database Library catalogue of the German National Library of Science and Technology (TIB), Hannover

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  7. Book ; Online ; Thesis: Cell-cell adhesion mediated by mobile receptor-ligand pairs

    Fenz, Susanne Franziska [Verfasser]

    a biomimetic study

    2009  

    Author's details vorgelegt von Susanne Franziska Fenz
    Keywords Biowissenschaften, Biologie ; Life Science, Biology
    Subject code sg570
    Language English
    Document type Book ; Online ; Thesis
    Database Digital theses on the web

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  8. Article: Cell-free synthesis of membrane proteins: tailored cell models out of microsomes.

    Fenz, Susanne F / Sachse, Rita / Schmidt, Thomas / Kubick, Stefan

    Biochimica et biophysica acta

    2014  Volume 1838, Issue 5, Page(s) 1382–1388

    Abstract: Incorporation of proteins in biomimetic giant unilamellar vesicles (GUVs) is one of the hallmarks towards cell models in which we strive to obtain a better mechanistic understanding of the manifold cellular processes. The reconstruction of transmembrane ... ...

    Abstract Incorporation of proteins in biomimetic giant unilamellar vesicles (GUVs) is one of the hallmarks towards cell models in which we strive to obtain a better mechanistic understanding of the manifold cellular processes. The reconstruction of transmembrane proteins, like receptors or channels, into GUVs is a special challenge. This procedure is essential to make these proteins accessible to further functional investigation. Here we describe a strategy combining two approaches: cell-free eukaryotic protein expression for protein integration and GUV formation to prepare biomimetic cell models. The cell-free protein expression system in this study is based on insect lysates, which provide endoplasmic reticulum derived vesicles named microsomes. It enables signal-induced translocation and posttranslational modification of de novo synthesized membrane proteins. Combining these microsomes with synthetic lipids within the electroswelling process allowed for the rapid generation of giant proteo-liposomes of up to 50 μm in diameter. We incorporated various fluorescent protein-labeled membrane proteins into GUVs (the prenylated membrane anchor CAAX, the heparin-binding epithelial growth factor like factor Hb-EGF, the endothelin receptor ETB, the chemokine receptor CXCR4) and thus presented insect microsomes as functional modules for proteo-GUV formation. Single-molecule fluorescence microscopy was applied to detect and further characterize the proteins in the GUV membrane. To extend the options in the tailoring cell models toolbox, we synthesized two different membrane proteins sequentially in the same microsome. Additionally, we introduced biotinylated lipids to specifically immobilize proteo-GUVs on streptavidin-coated surfaces. We envision this achievement as an important first step toward systematic protein studies on technical surfaces.
    MeSH term(s) Animals ; Biomimetic Materials/chemical synthesis ; Biomimetic Materials/metabolism ; Biomimetics/methods ; Cell-Free System ; Insecta ; Lipids/chemistry ; Liposomes/metabolism ; Membrane Proteins/chemical synthesis ; Membrane Proteins/metabolism ; Membranes/metabolism ; Microsomes/chemistry ; Microsomes/metabolism ; Models, Biological ; Unilamellar Liposomes/chemistry ; Unilamellar Liposomes/metabolism
    Chemical Substances Lipids ; Liposomes ; Membrane Proteins ; Unilamellar Liposomes
    Language English
    Publishing date 2014-05
    Publishing country Netherlands
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 60-7
    ISSN 1879-2596 ; 1879-260X ; 1872-8006 ; 1879-2642 ; 1879-2618 ; 1879-2650 ; 0006-3002 ; 0005-2728 ; 0005-2736 ; 0304-4165 ; 0167-4838 ; 1388-1981 ; 0167-4889 ; 0167-4781 ; 0304-419X ; 1570-9639 ; 0925-4439 ; 1874-9399
    ISSN (online) 1879-2596 ; 1879-260X ; 1872-8006 ; 1879-2642 ; 1879-2618 ; 1879-2650
    ISSN 0006-3002 ; 0005-2728 ; 0005-2736 ; 0304-4165 ; 0167-4838 ; 1388-1981 ; 0167-4889 ; 0167-4781 ; 0304-419X ; 1570-9639 ; 0925-4439 ; 1874-9399
    DOI 10.1016/j.bbamem.2013.12.009
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  9. Book ; Online ; Thesis: The role of nuclear architecture in the context of antigenic variation in Trypanosoma brucei

    Müller, Laura S. M. [Verfasser] / Siegel, T. Nicolai [Gutachter] / Engstler, Markus [Gutachter] / Förstner, Konrad U. [Gutachter] / Fenz, Susanne [Gutachter]

    2020  

    Author's details Laura Müller-Hübner ; Gutachter: T. Nicolai Siegel, Markus Engstler, Konrad U. Förstner, Susanne Fenz
    Keywords Medizin, Gesundheit ; Medicine, Health
    Subject code sg610
    Language English
    Publisher Universität Würzburg
    Publishing place Würzburg
    Document type Book ; Online ; Thesis
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  10. Article ; Online: Facilitating trypanosome imaging.

    Glogger, Marius / Subota, Ines / Pezzarossa, Anna / Denecke, Anna-Lena / Carrington, Mark / Fenz, Susanne F / Engstler, Markus

    Experimental parasitology

    2017  Volume 180, Page(s) 13–18

    Abstract: Research on trypanosomes as a model organism has provided a substantial contribution to a detailed understanding of basic cellular processes within the last few years. At the same time, major advances in super-resolution microscopy have been achieved, ... ...

    Abstract Research on trypanosomes as a model organism has provided a substantial contribution to a detailed understanding of basic cellular processes within the last few years. At the same time, major advances in super-resolution microscopy have been achieved, facilitating the resolution of biological structures in living cells at a scale of a few nm. However, the motility of trypanosomes has prevented access to high resolution microscopy of live cells. Here, we present a hydrogel based on poly(ethylene glycol) functionalized with either norbornene or thiol moieties for UV induced thiol-ene crosslinking for the embedding and imaging of live trypanosomes. The resulting gel exhibits low autofluorescence properties, immobilizes the cells efficiently on the nanometer scale and is compatible with cell viability for up to one hour at 24 °C. We applied super-resolution imaging to the inner plasma membrane leaflet using lipid-anchored eYFP as a probe. We find specific domains within the membrane where the fluorescence either accumulates or appears diluted rather than being homogenously distributed. Based on a Ripley's analysis, the size of the domains was determined to be r
    MeSH term(s) Antigens, Protozoan/chemistry ; Antigens, Protozoan/metabolism ; Bacterial Proteins/chemistry ; Bacterial Proteins/metabolism ; Biomarkers/chemistry ; Biomarkers/metabolism ; Cell Membrane/ultrastructure ; Fluorescent Antibody Technique ; Green Fluorescent Proteins/chemistry ; Green Fluorescent Proteins/metabolism ; Hydrogels ; Luminescent Proteins/chemistry ; Luminescent Proteins/metabolism ; Microscopy, Fluorescence ; Protozoan Proteins/chemistry ; Protozoan Proteins/metabolism ; Trypanosoma brucei brucei/cytology ; Trypanosoma brucei brucei/genetics ; Trypanosoma brucei brucei/ultrastructure
    Chemical Substances Antigens, Protozoan ; Bacterial Proteins ; Biomarkers ; HASPB protein, Leishmania ; Hydrogels ; Luminescent Proteins ; Protozoan Proteins ; yellow fluorescent protein, Bacteria ; Green Fluorescent Proteins (147336-22-9)
    Language English
    Publishing date 2017-09
    Publishing country United States
    Document type Journal Article
    ZDB-ID 391089-1
    ISSN 1090-2449 ; 0014-4894
    ISSN (online) 1090-2449
    ISSN 0014-4894
    DOI 10.1016/j.exppara.2017.03.010
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