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  1. Article ; Online: Analysis of

    Fernández-Fernández, Rocío / López-Igual, Rocío / Casadesús, Josep / Sánchez-Romero, María Antonia

    Frontiers in cellular and infection microbiology

    2023  Volume 13, Page(s) 1146070

    Abstract: Microbial cell individuality is receiving increasing interest in the scientific community. Individual cells within clonal populations exhibit noticeable phenotypic heterogeneity. The advent of fluorescent protein technology and advances in single-cell ... ...

    Abstract Microbial cell individuality is receiving increasing interest in the scientific community. Individual cells within clonal populations exhibit noticeable phenotypic heterogeneity. The advent of fluorescent protein technology and advances in single-cell analysis has revealed phenotypic cell variant in bacterial populations. This heterogeneity is evident in a wide range of phenotypes, for example, individual cells display variable degrees of gene expression and survival under selective conditions and stresses, and can exhibit differing propensities to host interactions. Last few years, numerous cell sorting approaches have been employed for resolving the properties of bacterial subpopulations. This review provides an overview of applications of cell sorting to analyze
    MeSH term(s) Salmonella/genetics ; Phenotype ; Bacteria ; Gene Expression Profiling
    Language English
    Publishing date 2023-03-29
    Publishing country Switzerland
    Document type Journal Article ; Review ; Research Support, Non-U.S. Gov't
    ZDB-ID 2619676-1
    ISSN 2235-2988 ; 2235-2988
    ISSN (online) 2235-2988
    ISSN 2235-2988
    DOI 10.3389/fcimb.2023.1146070
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: Identifying Bacterial Lineages in Salmonella by Flow Cytometry.

    Fernández-Fernández, Rocío / Olivenza, David R / Sánchez-Romero, María Antonia

    EcoSal Plus

    2022  , Page(s) eESP00182021

    Abstract: Advances in technologies that permit high-resolution analysis of events in single cells have revealed that phenotypic heterogeneity is a widespread phenomenon in bacteria. Flow cytometry has the potential to describe the distribution of cellular ... ...

    Abstract Advances in technologies that permit high-resolution analysis of events in single cells have revealed that phenotypic heterogeneity is a widespread phenomenon in bacteria. Flow cytometry has the potential to describe the distribution of cellular properties within a population of bacterial cells and has yielded invaluable information about the ability of isogenic cells to diversify into phenotypic subpopulations. This review will discuss several single-cell approaches that have recently been applied to define phenotypic heterogeneity in populations of Salmonella enterica.
    Language English
    Publishing date 2022-02-11
    Publishing country United States
    Document type Journal Article
    ISSN 2324-6200
    ISSN (online) 2324-6200
    DOI 10.1128/ecosalplus.ESP-0018-2021
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article ; Online: Transcription-driven DNA supercoiling counteracts H-NS-mediated gene silencing in bacterial chromatin.

    Figueroa-Bossi, Nara / Fernández-Fernández, Rocío / Kerboriou, Patricia / Bouloc, Philippe / Casadesús, Josep / Sánchez-Romero, María Antonia / Bossi, Lionello

    Nature communications

    2024  Volume 15, Issue 1, Page(s) 2787

    Abstract: In all living cells, genomic DNA is compacted through interactions with dedicated proteins and/or the formation of plectonemic coils. In bacteria, DNA compaction is achieved dynamically, coordinated with dense and constantly changing transcriptional ... ...

    Abstract In all living cells, genomic DNA is compacted through interactions with dedicated proteins and/or the formation of plectonemic coils. In bacteria, DNA compaction is achieved dynamically, coordinated with dense and constantly changing transcriptional activity. H-NS, a major bacterial nucleoid structuring protein, is of special interest due to its interplay with RNA polymerase. H-NS:DNA nucleoprotein filaments inhibit transcription initiation by RNA polymerase. However, the discovery that genes silenced by H-NS can be activated by transcription originating from neighboring regions has suggested that elongating RNA polymerases can disassemble H-NS:DNA filaments. In this study, we present evidence that transcription-induced counter-silencing does not require transcription to reach the silenced gene; rather, it exerts its effect at a distance. Counter-silencing is suppressed by introducing a DNA gyrase binding site within the intervening segment, suggesting that the long-range effect results from transcription-driven positive DNA supercoils diffusing toward the silenced gene. We propose a model wherein H-NS:DNA complexes form in vivo on negatively supercoiled DNA, with H-NS bridging the two arms of the plectoneme. Rotational diffusion of positive supercoils generated by neighboring transcription will cause the H-NS-bound negatively-supercoiled plectoneme to "unroll" disrupting the H-NS bridges and releasing H-NS.
    MeSH term(s) Chromatin/metabolism ; DNA-Binding Proteins/metabolism ; Bacterial Proteins/genetics ; Bacterial Proteins/metabolism ; Bacteria/genetics ; DNA-Directed RNA Polymerases/genetics ; DNA-Directed RNA Polymerases/metabolism ; DNA/metabolism ; Gene Silencing ; Gene Expression Regulation, Bacterial ; DNA, Bacterial/genetics ; DNA, Bacterial/metabolism ; Transcription, Genetic
    Chemical Substances Chromatin ; DNA-Binding Proteins ; Bacterial Proteins ; DNA-Directed RNA Polymerases (EC 2.7.7.6) ; DNA (9007-49-2) ; DNA, Bacterial
    Language English
    Publishing date 2024-03-30
    Publishing country England
    Document type Journal Article
    ZDB-ID 2553671-0
    ISSN 2041-1723 ; 2041-1723
    ISSN (online) 2041-1723
    ISSN 2041-1723
    DOI 10.1038/s41467-024-47114-w
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article: Evidence for Involvement of the

    Fernández-Fernández, Rocío / Hernández, Sara B / Puerta-Fernández, Elena / Sánchez-Romero, María A / Urdaneta, Verónica / Casadesús, Josep

    Frontiers in microbiology

    2021  Volume 12, Page(s) 647305

    Abstract: Genes annotated ... ...

    Abstract Genes annotated as
    Language English
    Publishing date 2021-02-25
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2587354-4
    ISSN 1664-302X
    ISSN 1664-302X
    DOI 10.3389/fmicb.2021.647305
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article ; Online: Use of monoclonal antibodies for metastatic colorectal cancer in the Andalusian public health system.

    Santos-Ramos, Bernardo / Fernández-Fernández, Rocío / Marín-Gil, Roberto / Espinosa-Bosch, María / Peiró-Moreno, Salvador / Pérez-Guerrero, Concepción / Bautista-Paloma, Javier

    International journal of clinical pharmacy

    2013  Volume 35, Issue 4, Page(s) 550–553

    Abstract: Background: The place of monoclonal antibodies in metastatic colorectal cancer has not been clearly defined.: Objective: To determine the treatment pattern of monoclonal antibodies in colorectal cancer patients in the Andalusian Public Healthcare ... ...

    Abstract Background: The place of monoclonal antibodies in metastatic colorectal cancer has not been clearly defined.
    Objective: To determine the treatment pattern of monoclonal antibodies in colorectal cancer patients in the Andalusian Public Healthcare System.
    Method: Data were collected from all patients treated with these drugs from July 2009 to December 2010 from pharmacy programs and medical records.
    Results: Three hundred patients were included, of whom 227 received the antibody at the forefront. The proportion of patients who received bevacizumab in the first line is greater than that of cetuximab (62.1 vs. 37.5 % respectively) and similar in the second line and subsequent (47.8 vs. 53.8 % and 48.5 vs. 46.2 % respectively). XELOXbevacizumab was the most frequently prescribed scheme (35.3 %) followed by FOLFOX-monoclonal antibody schemes, regardless that this was bevacizumab or cetuximab (22.5 %). The median progression free survival (PFS) was 11.7 months for patients receiving cetuximab, 9.6 months for patients receiving bevacizumab and 8.2 months for those who received no monoclonal antibody in the first line.
    Conclusion: Bevacizumab was the antibody of choice in first line, showing utilization rates similar to cetuximab in second line and subsequent. The median PFS in our study is related to the PFS of the major clinical trials.
    MeSH term(s) Aged ; Angiogenesis Inhibitors/administration & dosage ; Antibodies, Monoclonal/administration & dosage ; Antibodies, Monoclonal/therapeutic use ; Antibodies, Monoclonal, Humanized/administration & dosage ; Antineoplastic Combined Chemotherapy Protocols/administration & dosage ; Antineoplastic Combined Chemotherapy Protocols/therapeutic use ; Bevacizumab ; Cetuximab ; Colorectal Neoplasms/drug therapy ; Colorectal Neoplasms/pathology ; Disease-Free Survival ; Humans ; Medical Records ; Middle Aged ; Neoplasm Metastasis ; Panitumumab ; Spain
    Chemical Substances Angiogenesis Inhibitors ; Antibodies, Monoclonal ; Antibodies, Monoclonal, Humanized ; Bevacizumab (2S9ZZM9Q9V) ; Panitumumab (6A901E312A) ; Cetuximab (PQX0D8J21J)
    Language English
    Publishing date 2013-05-15
    Publishing country Netherlands
    Document type Comparative Study ; Journal Article ; Multicenter Study ; Observational Study ; Research Support, Non-U.S. Gov't
    ZDB-ID 2601204-2
    ISSN 2210-7711 ; 2210-7703 ; 0928-1231
    ISSN (online) 2210-7711
    ISSN 2210-7703 ; 0928-1231
    DOI 10.1007/s11096-013-9791-y
    Database MEDical Literature Analysis and Retrieval System OnLINE

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