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Article ; Online: Initial Steps of XY Sex Chromosome Differentiation in the Armored Catfish

Balini, Ligia Carla / Fernandes, Carlos Alexandre / Portela-Castro, Ana Luiza de Brito / Melo, Rafael Fernando de / Zawadzki, Cláudio Henrique / Borin-Carvalho, Luciana Andreia

Zebrafish

2024

Abstract: In fish species, heterochromatinization is one process that could trigger sex chromosome differentiation. The present article describes a nascent XX/XY sex chromosome system evidenced by heterochromatin accumulation and microsatellite (GATA) ...

Abstract	In fish species, heterochromatinization is one process that could trigger sex chromosome differentiation. The present article describes a nascent XX/XY sex chromosome system evidenced by heterochromatin accumulation and microsatellite (GATA)
Language	English
Publishing date	2024-02-20
Publishing country	United States
Document type	Journal Article
ZDB-ID	2156020-1
ISSN	1557-8542 ; 1545-8547
ISSN (online)	1557-8542
ISSN	1545-8547
DOI	10.1089/zeb.2023.0100
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Article ; Online: Genotoxicity and cytotoxicity of textile production effluents, before and after Bacillus subitilis bioremediation, in Astyanax lacustris (Pisces, Characidae).

da Silva, Josiane Rodrigues Rocha / Gregorio, Angelivia / Portela-Castro, Ana Luiza de Brito / Fernandes, Carlos Alexandre

Mutation research. Genetic toxicology and environmental mutagenesis

2023 Volume 886, Page(s) 503588

Abstract: Textile effluents may be highly toxic and mutagenic. Monitoring studies are important for sustaining the aquatic ecosystems contaminated by these materials, which can cause damage to organisms and loss of biodiversity. We have evaluated the cyto- and ... ...

Abstract	Textile effluents may be highly toxic and mutagenic. Monitoring studies are important for sustaining the aquatic ecosystems contaminated by these materials, which can cause damage to organisms and loss of biodiversity. We have evaluated the cyto- and genotoxicity of textile effluents on erythrocytes of Astyanax lacustris, before and after bioremediation by Bacillus subitilis treatment. We tested 60 fish (five treatment conditions, four fish per condition, in triplicate). Fish were exposed to contaminants for 7 days. The assays used were biomarker analysis, the micronucleus (MN) test, analysis of cellular morphological changes (CMC), and the comet assay. All concentrations of effluent tested, and the bioremediated effluent, showed damage significantly different from the controls. We conclude that water pollution assessment can be accomplished with these biomarkers. Biodegradation of the textile effluent was only partial, indicating the need for more thorough bioremediation to effect complete neutralization of toxicity.
MeSH term(s)	Animals ; Characidae ; Biodegradation, Environmental ; Ecosystem ; Bacillus ; Textiles
Language	English
Publishing date	2023-01-16
Publishing country	Netherlands
Document type	Journal Article
ISSN	1879-3592
ISSN (online)	1879-3592
DOI	10.1016/j.mrgentox.2023.503588
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Article ; Online: Assessment of the attributes of Primary Health Care with women of reproductive age.

Martins, Débora Cristina / Silva, Giordana Maronezzi da / Pesce, Giovanna Brichi / Fernandes, Carlos Alexandre Molena

Revista brasileira de enfermagem

2022 Volume 75, Issue 3, Page(s) e20210015

Abstract: Objectives: to evaluate the quality of Primary Health Care attributes according to the characterization and perspective of women of reproductive age.: Methods: descriptive, quantitative, cross-sectional study, with 397 women of reproductive age from ... ...

Abstract	Objectives: to evaluate the quality of Primary Health Care attributes according to the characterization and perspective of women of reproductive age. Methods: descriptive, quantitative, cross-sectional study, with 397 women of reproductive age from six municipalities in the 16th Health Region of the state of Paraná, with variables for sociodemographic characterization and the Primary Care Assessment Tool (PCATool). Results: mean age, 30.43 years old, most were married, 70.2% worked outside the home and 55.5% had completed high school. The highest average score of the eight domains evaluated was "Family Guidance" and "Coordination - Integration of Care", with averages of 5.86 and 4.89. Domains "Integrality - Services Provided" and "Community Orientation" had lower averages (3.32 and 3.76). Conclusions: the study allowed tracing the characterization of the participants and identifying that the attributes of Primary Care are unsatisfactory, making it necessary to expand access to the services offered and to qualify the comprehensive care for women's health.
MeSH term(s)	Adult ; Brazil ; Comprehensive Health Care ; Cross-Sectional Studies ; Female ; Humans ; Primary Health Care ; Reproduction
Language	Portuguese
Publishing date	2022-02-25
Publishing country	Brazil
Document type	Journal Article
ZDB-ID	731983-6
ISSN	1984-0446 ; 0034-7167
ISSN (online)	1984-0446
ISSN	0034-7167
DOI	10.1590/0034-7167-2021-0015
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Article ; Online: Hospitalizations for mental and behavioral disorders due to alcohol use in Brazil and regions: a temporal trend analysis, 2010-2020.

Oliveira, Renata Savian Colvero de / Matias, Jéssica Campos / Fernandes, Carlos Alexandre Ortiz Raifone / Gavioli, Aroldo / Marangoni, Sônia Regina / Assis, Fátima Büchele

Epidemiologia e servicos de saude : revista do Sistema Unico de Saude do Brasil

2023 Volume 32, Issue 1, Page(s) e20211266

Abstract: Objective: to analyze the trend in hospitalization rates for mental and behavioral disorders due to alcohol use (ICD-10: F10.0), Brazil and its five regions, 2010-2020.: Methods: this was an ecological time-series study using data from the Hospital ... ...

Abstract	Objective: to analyze the trend in hospitalization rates for mental and behavioral disorders due to alcohol use (ICD-10: F10.0), Brazil and its five regions, 2010-2020. Methods: this was an ecological time-series study using data from the Hospital Information System of the Brazilian National Health System; to calculate the trend in hospitalizations, we used the Prais-Winsten generalized linear regression model and the Stata Statistical Software 14.0; a decreasing trend was considered when p-value < 0.05, and the regression coefficient was negative. Results: in the period analyzed, there were 423,290 hospitalizations for mental and behavioral disorders due to alcohol use in the country; the data analyzed showed a decreasing trend in adult hospitalization rates for this cause in the country (p-value < 0.001; 95%CI -0.094;-0.079) and in all its regions, for both sexes (p-value < 0.001). Conclusion: hospitalizations for mental and behavioral disorders due to alcohol use have reduced in Brazil and in its macro-regions.
MeSH term(s)	Male ; Adult ; Female ; Humans ; Brazil/epidemiology ; Time Factors ; Mental Disorders/epidemiology ; Linear Models ; Hospitalization
Language	Portuguese
Publishing date	2023-03-17
Publishing country	Brazil
Document type	Journal Article
ZDB-ID	2880588-4
ISSN	2237-9622 ; 1679-4974
ISSN (online)	2237-9622
ISSN	1679-4974
DOI	10.1590/S2237-96222023000100005
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Article ; Online: Hidden Diversity in

Mazetto, Raissa Caroline / Ramos, Laura Ivana / Vieira, Margarida Maria de Rossi / Carvalho, Fernando Rogério / de Carvalho, Luciana Andréia Borin / de Oliveira, Alessandra Valéria / Fernandes, Carlos Alexandre / Portela Castro, Ana Luiza de Brito

Zebrafish

2024 Volume 21, Issue 1, Page(s) 39–47

Abstract: ... ...

Abstract	Eigenmannia
MeSH term(s)	Humans ; Animals ; Gymnotiformes/genetics ; Brazil ; Rivers ; Paraguay ; Zebrafish ; Phylogeny
Language	English
Publishing date	2024-01-29
Publishing country	United States
Document type	Journal Article
ZDB-ID	2156020-1
ISSN	1557-8542 ; 1545-8547
ISSN (online)	1557-8542
ISSN	1545-8547
DOI	10.1089/zeb.2023.0107
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Article ; Online: Erythrocyte alterations in specimens of Danio rerio caused by exposure to metformin.

Barbieri, Pablo Americo / Lupepsa, Luara / Paupitz, Brennda Ribeiro / de Andrade, Camila Oliveira / Gigliolli, Adriana Aparecida Sinópolis / de Brito Portela-Castro, Ana Luiza / Ribeiro, Ricardo Pereira / Fernandes, Carlos Alexandre / Borin-Carvalho, Luciana Andreia

Environmental toxicology and pharmacology

2024 Volume 106, Page(s) 104373

Abstract: The antidiabetic drug metformin is widely prescribed around the world. However, its permanence in different environmental concentrations has been associated with adverse toxicological effects in organisms that do not target its therapeutic action. In the ...

Abstract	The antidiabetic drug metformin is widely prescribed around the world. However, its permanence in different environmental concentrations has been associated with adverse toxicological effects in organisms that do not target its therapeutic action. In the aquatic environment, fish such as the Zebrafish (Danio rerio) have been considered potential bioindicators of environmental impacts and used as experimental models in toxicological studies due to the sensitivity of these species to different types of contaminants, including pharmaceuticals. Thus, this study aimed to analyze metformin's cytotoxic effects on Danio rerio erythrocytes. The animals were submitted to different concentrations of the drug (50 µg/L, 100 µg/L, 150 µg/L, and 10000 µg/L) for 365 days and subsequently observed employing light microscopy and scanning electron microscopy (SEM) to evaluate the alterations that occurred. Exposure of animals to metformin led to significant erythrocyte cell abnormalities across all tested concentrations, with a particularly pronounced effect at the higher concentration previously defined as the NOEC (No Observed Effect Concentration). Remarkable abnormalities included cytoplasmic vacuoles, echinocytes, and vesicle-like cytoplasmic fragments. These findings suggest that metformin, even at concentrations similar to those found in nature and at the NOEC level, exhibits cytotoxic potential in D. rerio, raising concerns about its potential health impacts.
MeSH term(s)	Animals ; Zebrafish ; Water Pollutants, Chemical/toxicity ; Metformin/toxicity ; Erythrocytes
Chemical Substances	Water Pollutants, Chemical ; Metformin (9100L32L2N)
Language	English
Publishing date	2024-01-18
Publishing country	Netherlands
Document type	Journal Article
ZDB-ID	1318302-3
ISSN	1872-7077 ; 1382-6689
ISSN (online)	1872-7077
ISSN	1382-6689
DOI	10.1016/j.etap.2024.104373
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Article ; Online: Multiprofessional intervention and telenursing in the treatment of obese people in the COVID-19 pandemic: a pragmatic clinical trial.

Christinelli, Heloá Costa Borim / Westphal, Greice / Costa, Maria Antonia Ramos / Okawa, Rogério Toshiro Passos / Nardo Junior, Nelson / Fernandes, Carlos Alexandre Molena

Revista brasileira de enfermagem

2022 Volume 75Suppl 2, Issue Suppl 2, Page(s) e20210059

Abstract: Objectives: to analyze the effects of a multiprofessional remote intervention and telenursing in the treatment of obesity.: Methods: pragmatic clinical trial of intervention, conducted with obese adults. The multiprofessional remote intervention with ...

Abstract	Objectives: to analyze the effects of a multiprofessional remote intervention and telenursing in the treatment of obesity. Methods: pragmatic clinical trial of intervention, conducted with obese adults. The multiprofessional remote intervention with a nurse occurred for 16 weeks through a messaging application. The application analyzed body composition, hemodynamic and laboratory variables, comparing the results obtained with the group that participated in face-to-face multiprofessional intervention without a nurse. The study investigated the effects of the intervention comparing the intervention groups and the moments through the ANOVA test for repeated measures. Results: Group 1 obtained significance in the variables: percentage of body fat (p = 0.008); blood glucose (p = 0.014); insulin (p = 0.001); abdominal and waist circumference; and HDL cholesterol (p = 0.000). Conclusions: the effects of multiprofessional remote intervention and telenursing significantly decreased the risk variables for metabolic syndrome in the treatment of obesity.
MeSH term(s)	Adult ; COVID-19 ; Humans ; Metabolic Syndrome/complications ; Metabolic Syndrome/therapy ; Obesity/complications ; Obesity/therapy ; Pandemics ; Telenursing
Language	Portuguese
Publishing date	2022-04-22
Publishing country	Brazil
Document type	Journal Article
ZDB-ID	731983-6
ISSN	1984-0446 ; 0034-7167
ISSN (online)	1984-0446
ISSN	0034-7167
DOI	10.1590/0034-7167-2021-0059
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Article: Integrated analysis reveals a new species of Corydoras Lacépède, 1803 (Siluriformes: Callichthyidae) in the lower Iguassu River, Brazil

da Rocha, Rafael Henrique / Fernandes, Carlos Alexandre / Bignotto, Thaís Souto / Margarido, Vladimir Pavan / Tencatt, Luiz Fernando Caserta / da Graça, Weferson Júnio / Gubiani, Éder André

Organisms, diversity, & evolution. 2022 June, v. 22, no. 2

2022

Abstract: Corydoras is the richest genus of Corydoradinae, and many of its species have not been identified to date. We characterized Corydoras carlae and Corydoras sp. by performing cytogenetic, morphometric, and molecular analyses to facilitate correct ... ...

Abstract	Corydoras is the richest genus of Corydoradinae, and many of its species have not been identified to date. We characterized Corydoras carlae and Corydoras sp. by performing cytogenetic, morphometric, and molecular analyses to facilitate correct identification and species delimitation and contribute an understanding of the evolutionary process of this group of fish. Individuals of C. carlae were collected in the Florido River, a tributary of the Iguassu River upstream of Iguassu Falls, and individuals of Corydoras sp. were collected in the Poço Preto River, a tributary of the Iguassu River downstream of Iguassu Falls. Corydoras sp. presented an extra rDNA 5S marker in an interstitial position on the short arm of one of the chromosomes of the submetacentric pair 15. Mitochondrial (COI) and nuclear (RAG1) sequences were efficient in discriminating C. carlae and Corydoras sp. Both species had exclusive haplotypes, which suggests the absence of gene flow between species. Furthermore, species delimitation analysis (GMYC and ABGD) suggested two MOTUs for Corydoras specimens from the Iguassu River. Differences in morphometric proportions were also observed. Considering the data gathered in this study, C. carlae and Corydoras sp. comprise distinct evolutionary lineages that are probably undergoing a recent speciation process.
Keywords	Corydoras ; evolution ; fish ; gene flow ; haplotypes ; mitochondria ; morphometry ; new species ; rivers ; Brazil
Language	English
Dates of publication	2022-06
Size	p. 457-474.
Publishing place	Springer Berlin Heidelberg
Document type	Article
ZDB-ID	2040179-6
ISSN	1618-1077 ; 1439-6092
ISSN (online)	1618-1077
ISSN	1439-6092
DOI	10.1007/s13127-021-00534-8
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Article ; Online: Biochemical and structural characterization of the RT domain of Leishmania sp. telomerase reverse transcriptase.

da Silva, Vitor Luiz / de Paiva, Stephany Cacete / de Oliveira, Hamine Cristina / Fernandes, Carlos Alexandre H / Salvador, Guilherme Henrique Marchi / Fontes, Marcos Roberto de M / Cano, Maria Isabel N

Biochimica et biophysica acta. General subjects

2023 Volume 1867, Issue 11, Page(s) 130451

Abstract: Background: The Leishmania genus comprises parasites that cause leishmaniasis, a neglected disease spread worldwide. Leishmania sp. telomeres are composed of TTAGGG repeats maintained by telomerase. In most eukaryotes, the enzyme minimal complex ... ...

Abstract	Background: The Leishmania genus comprises parasites that cause leishmaniasis, a neglected disease spread worldwide. Leishmania sp. telomeres are composed of TTAGGG repeats maintained by telomerase. In most eukaryotes, the enzyme minimal complex contains the TER (telomerase RNA) and the TERT (telomerase reverse transcriptase) components. The TERT holds the enzyme catalytic core and is formed by four structural and functional domains (TEN, Telomerase Essential N-terminal; TRBD, Telomerase RNA Binding Domain; RT, the reverse transcriptase domain and CTE, C-Terminal Extension domain). Methods and results: Amino acid sequence alignments, protein structure prediction analysis, and protein: nucleic acid interaction assays were used to show that the Leishmania major RT domain preserves the canonical structural elements found in higher eukaryotes, including the canonical motifs and the aspartic acid residues that stabilize the Mg Conclusion: Our results highlight that the telomerase catalysis mechanism is conserved in a pathogen of medical importance despite the structural peculiarities present in the parasite's RT domain.
MeSH term(s)	Animals ; Telomerase/chemistry ; Parasites/genetics ; Parasites/metabolism ; Leishmania/genetics ; Nucleic Acid Conformation ; Catalytic Domain
Chemical Substances	Telomerase (EC 2.7.7.49)
Language	English
Publishing date	2023-09-24
Publishing country	Netherlands
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	60-7
ISSN	1872-8006 ; 1879-2596 ; 1879-260X ; 1879-2642 ; 1879-2618 ; 1879-2650 ; 0006-3002 ; 0005-2728 ; 0005-2736 ; 0304-4165 ; 0167-4838 ; 1388-1981 ; 0167-4889 ; 0167-4781 ; 0304-419X ; 1570-9639 ; 0925-4439 ; 1874-9399
ISSN (online)	1872-8006 ; 1879-2596 ; 1879-260X ; 1879-2642 ; 1879-2618 ; 1879-2650
ISSN	0006-3002 ; 0005-2728 ; 0005-2736 ; 0304-4165 ; 0167-4838 ; 1388-1981 ; 0167-4889 ; 0167-4781 ; 0304-419X ; 1570-9639 ; 0925-4439 ; 1874-9399
DOI	10.1016/j.bbagen.2023.130451
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Article ; Online: Exploring New Tools for Risk Classification among Adults with Several Degrees of Obesity.

Westphal-Nardo, Greice / Chaput, Jean-Philippe / Faúndez-Casanova, César / Fernandes, Carlos Alexandre Molena / de Andrade Gonçalves, Eliane Cristina / Utrila, Raquel Tomiazzi / Oltramari, Karine / Grizzo, Felipe Merchan Ferraz / Nardo-Junior, Nelson

International journal of environmental research and public health

2023 Volume 20, Issue 13

Abstract: The epidemic of obesity worldwide has been recognized as a very important challenge. Within its complexity, the identification of higher-risk patients is essential, as it is unsustainable to offer access to treatment to all people with obesity. Several ... ...

Abstract	The epidemic of obesity worldwide has been recognized as a very important challenge. Within its complexity, the identification of higher-risk patients is essential, as it is unsustainable to offer access to treatment to all people with obesity. Several new approaches have recently been presented as important tools for risk stratification. In this research, we applied several of these tools in a cross-sectional study involving adults with obesity classes I, II, III, and super-obesity. The participants had their cardiometabolic risk profiles assessed. The study included adults with obesity aged 18 to 50 years (
MeSH term(s)	Humans ; Adult ; Male ; Metabolic Syndrome/epidemiology ; Cross-Sectional Studies ; Body Mass Index ; Obesity/complications ; Risk Factors ; Cardiovascular Diseases/etiology ; Waist Circumference
Language	English
Publishing date	2023-06-30
Publishing country	Switzerland
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2175195-X
ISSN	1660-4601 ; 1661-7827
ISSN (online)	1660-4601
ISSN	1661-7827
DOI	10.3390/ijerph20136263
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