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  1. Article ; Online: Short Peripheral Venous Catheters Contamination and the Dangers of Bloodstream Infection in Portugal

    Nádia Osório / Vânia Oliveira / Maria Inês Costa / Paulo Santos-Costa / Beatriz Serambeque / Fernando Gama / David Adriano / João Graveto / Pedro Parreira / Anabela Salgueiro-Oliveira

    Microorganisms, Vol 11, Iss 709, p

    An Analytic Study

    2023  Volume 709

    Abstract: Peripheral venous catheters (PVCs) are the most used vascular access devices in the world. However, failure rates remain considerably high, with complications such as PVC-related infections posing significant threats to patients’ well-being. In Portugal, ...

    Abstract Peripheral venous catheters (PVCs) are the most used vascular access devices in the world. However, failure rates remain considerably high, with complications such as PVC-related infections posing significant threats to patients’ well-being. In Portugal, studies evaluating the contamination of these vascular medical devices and characterizing the associated microorganisms are scarce and lack insight into potential virulence factors. To address this gap, we analyzed 110 PVC tips collected in a large tertiary hospital in Portugal. Experiments followed Maki et al.’s semi-quantitative method for microbiological diagnosis. Staphylococcus spp. were subsequently studied for the antimicrobial susceptibility profile by disc diffusion method and based on the cefoxitin phenotype, were further classified into strains resistant to methicillin. Screening for the mec A gene was also done by a polymerase chain reaction and minimum inhibitory concentration (MIC)-vancomycin as determined by E-test, proteolytic and hemolytic activity on skimmed milk 1% plate and blood agar, respectively. The biofilm formation was evaluated on microplate reading through iodonitrotetrazolium chloride 95% (INT). Overall, 30% of PVCs were contaminated, and the most prevalent genus was Staphylococcus spp., 48.8%. This genus presented resistance to penicillin (91%), erythromycin (82%), ciprofloxacin (64%), and cefoxitin (59%). Thus, 59% of strains were considered resistant to methicillin; however, we detected the mec A gene in 82% of the isolates tested. Regarding the virulence factors, 36.4% presented α-hemolysis and 22.7% β-hemolysis, 63.6% presented a positive result for the production of proteases, and 63.6% presented a biofilm formation capacity. Nearly 36.4% were simultaneously resistant to methicillin and showed expression of proteases and/or hemolysins, biofilm formation, and the MIC to vancomycin were greater than 2 µg/mL. Conclusion: PVCs were mainly contaminated with Staphylococcus spp., with high pathogenicity and resistance to antibiotics. ...
    Keywords peripheral intravenous catheter ; Staphylococcus spp ; virulence factors ; antibiotic resistance ; Biology (General) ; QH301-705.5
    Subject code 610
    Language English
    Publishing date 2023-03-01T00:00:00Z
    Publisher MDPI AG
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  2. Article ; Online: Environmental and agronomic assessment of soil conditioners produced from bauxite residue and oil palm wastes.

    Leite, Robson da Costa / Lucheta, Adriano Reis / Holanda, Roseanne Barata / Silva, Patricia Magalhães Pereira / Carmo, André Luiz Vilaça do / Gomes, Fernando Gama / Leite, Rubson da Costa / Melo, Caio Cesar Amorim de / Costa, Raphael Vieira da / Montini, Marcelo / Fernandes, Antonio Rodrigues

    Environmental research

    2023  Volume 233, Page(s) 116474

    Abstract: Soil conditioner is class of products used to enhance physics, physicochemical or soil biological activities, being able to recover disturbed or nutritional unbalanced soils. The formulation of a soil conditioner composed by bauxite residue (BR), and ... ...

    Abstract Soil conditioner is class of products used to enhance physics, physicochemical or soil biological activities, being able to recover disturbed or nutritional unbalanced soils. The formulation of a soil conditioner composed by bauxite residue (BR), and organic oil palm wastes, as raw materials, was recently proposed as an innovative strategy for the Brazilian acid soils amendment. Here we show the results of soil conditioner amended soil leaching tests and agronomical performance. The soil conditioners were formulated by BR mixed with decomposed POC (palm oil compost) and non-decomposed POMW (palm oil mill waste) oil palm wastes, in the proportion of 25% BR + 75% POC (T1) and 50% BR + 50% POMW (T2), in addition to the treatment with 100% POMW without BR (T3) and limestone at a dose calculated to raise soil pH to 6.0 (T4). Except for T4, all conditioners were applied to the soil at doses of 40, 80, and 120 t ha
    MeSH term(s) Soil/chemistry ; Aluminum Oxide ; Palm Oil ; Agriculture/methods ; Poaceae ; Soil Pollutants/analysis ; Calcium Carbonate
    Chemical Substances Soil ; Aluminum Oxide (LMI26O6933) ; Palm Oil (5QUO05548Z) ; Soil Pollutants ; Calcium Carbonate (H0G9379FGK)
    Language English
    Publishing date 2023-06-22
    Publishing country Netherlands
    Document type Journal Article
    ZDB-ID 205699-9
    ISSN 1096-0953 ; 0013-9351
    ISSN (online) 1096-0953
    ISSN 0013-9351
    DOI 10.1016/j.envres.2023.116474
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article ; Online: JUIZADOS ESPECIAIS CÍVEIS E AS GARANTIAS DO PROCESSO JUSTO

    Fernando Gama de Miranda Netto

    Revista da SJRJ, Vol 16, Iss 24, Pp 59-

    2010  Volume 74

    Abstract: O trabalho investiga os principais defeitos do microssistema dos juizados especiais cíveis. A partir do modelo constitucional de processo justo, examinam-se o princípio da paridade de armas no procedimento sumaríssimo e as garantias que podem ser ... ...

    Abstract O trabalho investiga os principais defeitos do microssistema dos juizados especiais cíveis. A partir do modelo constitucional de processo justo, examinam-se o princípio da paridade de armas no procedimento sumaríssimo e as garantias que podem ser renunciadas pelas partes, bem como a possibilidade de o magistrado retirar das partes mais garantias processuais, além das que o legislador expressamente subtraiu.
    Keywords Juizados Especiais Cíveis. Garantismo. Garantias do processo justo. ; Law in general. Comparative and uniform law. Jurisprudence ; K1-7720 ; Law ; K ; DOAJ:Law ; DOAJ:Law and Political Science
    Language Portuguese
    Publishing date 2010-05-01T00:00:00Z
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  4. Article ; Online: O tabu da relação do lobby e políticas públicas no Brasil

    Ana Claudia Santano / Fernando Gama de Miranda Netto / Luiz Alberto Blanchet

    Revista de Direito Econômico e Socioambiental, Vol 7, Iss 2, Pp 49-

    2016  Volume 72

    Abstract: Tema pouco estudado na literatura jurídica pátria, a pesquisa orienta-se pela possibilidade de o lobby trazer alguma contribuição para o desenvolvimento de políticas públicas no Brasil. Em tempos de desconfiança e de grande pressão exercidas sobre os ... ...

    Abstract Tema pouco estudado na literatura jurídica pátria, a pesquisa orienta-se pela possibilidade de o lobby trazer alguma contribuição para o desenvolvimento de políticas públicas no Brasil. Em tempos de desconfiança e de grande pressão exercidas sobre os poderes Executivo, Legislativo e Judiciário, torna-se importante analisar figuras marginalizadas e alvo de tabu no sistema, como os grupos de interesse, os grupos de pressão e o lobby. Neste sentido, fazendo uso do método bibliográfico, pretende-se estudar a relação existente entre esses fenômenos, bem como a outros aspectos que a eles estão vinculados. O trabalho finda por apresentar o lobby como um verdadeiro canal de comunicação entre as diversas esferas da organização política, ensejando a participação dos cidadãos em grupo das decisões do país, o que pode favorecer a transparência e até mesmo ao combate à corrupção.
    Keywords desenvolvimento ; políticas públicas ; interesse público ; participação política ; democracia ; Environmental sciences ; GE1-350 ; Law in general. Comparative and uniform law. Jurisprudence ; K1-7720
    Language English
    Publishing date 2016-07-01T00:00:00Z
    Publisher Pontifícia Universidade Católica do Paraná, Programa de Pós-Graduação em Direito
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  5. Article: Hospital microbial surface colonization revealed during monitoring of Klebsiella spp., Pseudomonas aeruginosa, and non-tuberculous mycobacteria

    Geadas Farias, Pedro / Fernando Gama / Diogo Reis / Susana Alarico / Nuno Empadinhas / José Carlos Martins / Ana Figueiredo de Almeida / Paula Vasconcelos Morais

    Antonie van Leeuwenhoek. 2017 July, v. 110, no. 7

    2017  

    Abstract: Hospital environmental conditions, human occupancy, and the characteristics of the equipment influence the survival of microbial communities and raise a concern with regard to nosocomial infections. The objective of the present work was to use the ... ...

    Abstract Hospital environmental conditions, human occupancy, and the characteristics of the equipment influence the survival of microbial communities and raise a concern with regard to nosocomial infections. The objective of the present work was to use the monitoring of Pseudomonas aeruginosa, Klebsiella spp. and non-tuberculous mycobacteria as a strategy to improve knowledge on microbial colonization of non-critical equipment and surfaces, in a tertiary hospital from Central Portugal. A 3-month microbiological survey was performed in a district teaching hospital. A total of 173 samples were obtained from the wards Hematology, Urology, Medicine, and Renal Transplants, and 102 presumptive strains recovered. Per sampling, Pseudomonas Isolation agar showed 42.8 to 73.3% of presumptive P. aeruginosa colonies and MacConkey agar recovered mostly Staphylococcus. Most of the colonies recovered in Middlebrook 7H10-PANTA belonged to the genus Methylobacterium. Taps and WC shower curtains carry high bacterial species diversity. The Redundancy Analysis grouped the samples in those mostly handled by patients, and those mostly handled by healthcare staff or of mixed use. This study shows that the preferential users of the space and equipment seem to be important contributors to the microbial community. The most recovered genus was Methylobacterium, known as colonizer of the water distribution system therefore, it is possible that the water points and biofilms in taps also contribute as dispersion hotspots.
    Keywords Klebsiella ; Methylobacterium ; Pseudomonas aeruginosa ; Staphylococcus ; agar ; biofilm ; environmental factors ; equipment ; health services ; hospitals ; humans ; microbial colonization ; microbial communities ; monitoring ; patients ; species diversity ; surveys ; water distribution ; Portugal
    Language English
    Dates of publication 2017-07
    Size p. 863-876.
    Publishing place Springer International Publishing
    Document type Article
    ZDB-ID 214861-4
    ISSN 1572-9699 ; 0003-6072
    ISSN (online) 1572-9699
    ISSN 0003-6072
    DOI 10.1007/s10482-017-0857-z
    Database NAL-Catalogue (AGRICOLA)

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  6. Article ; Online: Impact of an Innovative Securement Dressing and Tourniquet in Peripheral Intravenous Catheter-Related Complications and Contamination

    Pedro Parreira / Beatriz Serambeque / Paulo S. Costa / Lisete S. Mónico / Vânia Oliveira / Liliana B. Sousa / Fernando Gama / Rafael A. Bernardes / David Adriano / Inês A. Marques / Luciene M. Braga / João Graveto / Nádia Osório / Anabela Salgueiro-Oliveira

    International Journal of Environmental Research and Public Health, Vol 16, Iss 18, p

    An Interventional Study

    2019  Volume 3301

    Abstract: Reusable tourniquets and conventional securement dressings are considered risk factors for the occurrence of reported complications and catheter-related bloodstream infections. This study’s purpose is to assess the impact of single-use disposable ... ...

    Abstract Reusable tourniquets and conventional securement dressings are considered risk factors for the occurrence of reported complications and catheter-related bloodstream infections. This study’s purpose is to assess the impact of single-use disposable tourniquets and advanced occlusive polyurethane dressings with reinforced cloth borders on peripheral intravenous catheter (PIVC)-related complications and contamination. A pre- and post-interventional prospective observational study was conducted in a cardiology ward of a tertiary hospital between April 2018 and February 2019. Overall, demographic and clinical data from 156 patients and PIVC-related outcomes were collected ( n = 296) as well as PIVC tips for microbiological analysis ( n = 90). In the pre-intervention phase ( n = 118), complication rates of 62.1% were reported, while 44.1% of the PIVCs were contaminated ( n = 34). In the post-intervention phase ( n = 178), complication rates decreased to 57.3%, while contamination rates significantly decreased to 17.9% ( p = 0.014; n = 56). Through a logistic regression, it was found that the use of innovative technologies reduces the chance of PIVC contamination by 79% (odds ratio (OR): 0.21; 95% confidence interval (CI): 0.05−0.98; p = 0.046). Meanwhile, PIVC-related complications and fluid therapy emerged as predictors for PIVC contamination. Findings suggest that the adoption of these innovative devices in nurses’ practice contributes to the significant reduction of PIVC contamination.
    Keywords catheter-related bloodstream infections ; complications ; contamination ; infection prevention ; nursing ; occlusive dressings ; peripheral intravenous catheter ; tourniquets ; Medicine ; R
    Subject code 610
    Language English
    Publishing date 2019-09-01T00:00:00Z
    Publisher MDPI AG
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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