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  1. Article ; Online: A Prospective Comparison of Standard Technique, Doppler Ultrasonography, and Pressure Waveform Analysis for Confirming Intraosseous Catheter Placement.

    Greenstein, Yonatan Y / Ferrara, Scott / Jomi, Jerry / Soetanto, Vanessa / Zuckerman, Omry / Sena, Ariel / Ostrovsky, Ilya / Alerhand, Stephen

    Journal of intensive care medicine

    2023  Volume 39, Issue 3, Page(s) 222–229

    Abstract: Purpose: ...

    Abstract Purpose:
    MeSH term(s) Humans ; Catheters ; Ultrasonography ; Infusions, Intraosseous/methods ; Administration, Intravenous
    Language English
    Publishing date 2023-08-30
    Publishing country United States
    Document type Observational Study ; Journal Article
    ZDB-ID 632828-3
    ISSN 1525-1489 ; 0885-0666
    ISSN (online) 1525-1489
    ISSN 0885-0666
    DOI 10.1177/08850666231199086
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: The effect of semen processing on sperm DNA integrity: comparison of two techniques using the novel Toluidine Blue Assay.

    Marchesi, Dennis E / Biederman, Hannah / Ferrara, Scott / Hershlag, Avner / Feng, Huai L

    European journal of obstetrics, gynecology, and reproductive biology

    2010  Volume 151, Issue 2, Page(s) 176–180

    Abstract: Objective: The first goal of this study was to determine the effect that semen processing has on sperm DNA integrity. The second goal was to assess which processing technique (modified swim-up versus density gradient centrifugation) results in a ... ...

    Abstract Objective: The first goal of this study was to determine the effect that semen processing has on sperm DNA integrity. The second goal was to assess which processing technique (modified swim-up versus density gradient centrifugation) results in a superior sample. DNA integrity was measured using a novel Toluidine Blue Assay.
    Study design: Side-by-side comparison.
    Materials and methods: Raw semen samples were collected from thirty-two male individuals and scored for routine semen analysis. Prior to discarding the specimens identical aliquots were divided and processed by density gradient centrifugation and a modified swim-up technique. The Toluidine Blue Assay was used to analyze raw and processed samples.
    Results: Both density gradient centrifugation and the modified swim-up improved DNA quality compared to the unprocessed sample. However, the modified swim-up technique proved superior.
    Conclusions: The swim-up technique generates a sperm sample with better DNA integrity. Should DNA integrity correlate with better pregnancy rates in IUI and IVF, respectively, the swim-up may be the sperm processing technique of choice for these procedures.
    MeSH term(s) DNA/analysis ; DNA/physiology ; Fertilization in Vitro/methods ; Humans ; Male ; Semen/chemistry ; Semen/physiology ; Specimen Handling/methods ; Sperm Motility/physiology ; Spermatozoa/chemistry ; Spermatozoa/physiology ; Tolonium Chloride/chemistry
    Chemical Substances Tolonium Chloride (15XUH0X66N) ; DNA (9007-49-2)
    Language English
    Publishing date 2010-08
    Publishing country Ireland
    Document type Comparative Study ; Journal Article
    ZDB-ID 190605-7
    ISSN 1872-7654 ; 0301-2115 ; 0028-2243
    ISSN (online) 1872-7654
    ISSN 0301-2115 ; 0028-2243
    DOI 10.1016/j.ejogrb.2010.05.003
    Database MEDical Literature Analysis and Retrieval System OnLINE

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