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  1. Book ; Online: Fc-Mediated Antibody Functions and Fc-Receptor Polymorphism

    Ferrari, Guido / Tomaras, Georgia / Keith Reeves, R. / Scarlatti, Gabriella

    2020  

    Keywords Medicine ; Immunology ; Antibodies ; Fc-Mediated Antibody Functions ; Fc-Receptor ; Polymorphism ; FcR phenotypes ; FcR
    Size 1 electronic resource (273 pages)
    Publisher Frontiers Media SA
    Document type Book ; Online
    Note English ; Open Access
    HBZ-ID HT021231306
    ISBN 9782889638901 ; 2889638901
    Database ZB MED Catalogue: Medicine, Health, Nutrition, Environment, Agriculture

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  2. Article ; Online: Tandem bispecific broadly neutralizing antibody - a novel approach to HIV-1 treatment.

    Ferrari, Guido

    The Journal of clinical investigation

    2018  Volume 128, Issue 6, Page(s) 2189–2191

    Abstract: The last decade has led to a significant advance in our knowledge of HIV-1 latency and immunity. However, we are still not close to finding a cure for HIV-1. Although combination antiretroviral therapy (cART) has led to increased survival, almost close ... ...

    Abstract The last decade has led to a significant advance in our knowledge of HIV-1 latency and immunity. However, we are still not close to finding a cure for HIV-1. Although combination antiretroviral therapy (cART) has led to increased survival, almost close to that of the general population, it is still not curative. In the current issue of the JCI, Wu et al. studied the prophylactic and therapeutic potential of an engineered tandem bispecific broadly neutralizing antibody (bs-bnAb), BiIA-SG. This bnAb's breadth and potency were highly effective in protection and treatment settings, as measured by complete viremia control following direct infusion, as well as elimination of infected cells and delay in viral rebound when delivered with a recombinant vector. These observations underscore the need for the clinical development of BiIA-SG for the prevention of HIV-1.
    MeSH term(s) Animals ; Antibodies, Bispecific ; Antibodies, Neutralizing ; HIV Antibodies ; HIV Infections ; HIV-1/immunology ; Mice
    Chemical Substances Antibodies, Bispecific ; Antibodies, Neutralizing ; HIV Antibodies
    Language English
    Publishing date 2018-04-23
    Publishing country United States
    Document type Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't ; Comment
    ZDB-ID 3067-3
    ISSN 1558-8238 ; 0021-9738
    ISSN (online) 1558-8238
    ISSN 0021-9738
    DOI 10.1172/JCI121078
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Ua VI Zs.184: Show issues Location:
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    bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular
    ab Jg. 2022: Lesesaal (EG)

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  3. Article: Bispecific antibody-derived molecules to target persistent HIV infection.

    Nordstrom, Jeffrey L / Ferrari, Guido / Margolis, David M

    Journal of virus eradication

    2022  Volume 8, Issue 3, Page(s) 100083

    Abstract: HIV infection persists despite durable and potent antiviral therapy. To target persistent HIV infection, one major strategy aims to induce HIV provirus expression using latency reversing agents and then eliminate these reservoir cells via immune ... ...

    Abstract HIV infection persists despite durable and potent antiviral therapy. To target persistent HIV infection, one major strategy aims to induce HIV provirus expression using latency reversing agents and then eliminate these reservoir cells via immune responses enhanced by treatment with antibody-derived bispecific molecules. The specificities of anti-HIV-1 envelope monoclonal antibodies have been incorporated into bispecific molecules that can recognize infected cells and recruit cytotoxic immune cells to eliminate them. This concept seeks to engineer a unique and potent effector response based on the opportunity to target conserved viral epitopes on infected cells, and recruit broad populations of immune effector cells that are not limited by major histocompatibility complex restrictions or other programmed specificity constraints. This article provides a review of bispecific DART® molecules and other dual-specificity antibody-based molecules that function by co-engaging CD3-expressing T cells or CD16A-expressing NK cells with HIV-1-infected cells.
    Language English
    Publishing date 2022-08-28
    Publishing country England
    Document type Journal Article
    ZDB-ID 2868549-0
    ISSN 2055-6659 ; 2055-6640
    ISSN (online) 2055-6659
    ISSN 2055-6640
    DOI 10.1016/j.jve.2022.100083
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article ; Online: Engineering antibody-based molecules for HIV treatment and cure.

    Tuyishime, Marina / Ferrari, Guido

    Current opinion in HIV and AIDS

    2020  Volume 15, Issue 5, Page(s) 290–299

    Abstract: Purpose of review: Immunotherapy strategies alternative to current antiretroviral therapies will need to address viral diversity while increasing the immune system's ability to efficiently target the latent virus reservoir. Antibody-based molecules can ... ...

    Abstract Purpose of review: Immunotherapy strategies alternative to current antiretroviral therapies will need to address viral diversity while increasing the immune system's ability to efficiently target the latent virus reservoir. Antibody-based molecules can be designed based on broadly neutralizing and non-neutralizing antibodies that target free virions and infected cells. These multispecific molecules, either by IgG-like or non-IgG-like in structure, aim to target several independent HIV-1 epitopes and/or engage effector cells to eliminate the replicating virus and infected cells. This detailed review is intended to stimulate discussion on future requirements for novel immunotherapeutic molecules.
    Recent findings: Bispecific and trispecific antibodies are engineered as a single molecules to target two or more independent epitopes on the HIV-1 envelope (Env). These antibody-based molecules have increased avidity for Env, leading to improved neutralization potency and breadth compared with single parental antibodies. Furthermore, bispecific and trispecific antibodies that engage cellular receptors with one arm of the molecule help concentrate inhibitory molecules to the sites of potential infection and facilitate engagement of immune effector cells and Env-expressing target cells for their elimination.
    Summary: Recently engineered antibody-based molecules of different sizes and structures show promise in vitro or in vivo and are encouraging candidates for HIV treatment.
    MeSH term(s) Antibodies, Neutralizing ; Epitopes ; HIV Antibodies ; HIV Infections/drug therapy ; HIV-1/genetics ; Humans ; env Gene Products, Human Immunodeficiency Virus/genetics
    Chemical Substances Antibodies, Neutralizing ; Epitopes ; HIV Antibodies ; env Gene Products, Human Immunodeficiency Virus
    Language English
    Publishing date 2020-07-30
    Publishing country United States
    Document type Journal Article ; Research Support, N.I.H., Extramural ; Review
    ZDB-ID 2502511-9
    ISSN 1746-6318 ; 1746-630X
    ISSN (online) 1746-6318
    ISSN 1746-630X
    DOI 10.1097/COH.0000000000000640
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 6812: Show issues Location:
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  5. Article ; Online: Validation of the performance and suitability of a new class of FBS optimized for use in single-cell functional assays.

    Berrong, Mark / Ferrari, Guido / Porth, Cassie / Davis, Devin / Denny, Thomas / Janetzki, Sylvia / Rountree, Wes

    Journal of immunological methods

    2023  Volume 515, Page(s) 113452

    Abstract: The use of conventional serum for supplementation of media in cell-based and single-cell functional assays has been a major challenge for assay performance, standardization, optimization, and reproducibility. It has been identified as the leading cause ... ...

    Abstract The use of conventional serum for supplementation of media in cell-based and single-cell functional assays has been a major challenge for assay performance, standardization, optimization, and reproducibility. It has been identified as the leading cause of variability and suboptimal performance in large, international Elispot proficiency panels (Janetzki et al., 2008; Rountree et al., 2016). Extensive pretesting and optimization activities are one approach to overcome these challenges, but they are time-consuming and resource-intensive because suitable lots of serum are difficult to identify and secure in sufficient quantities to provide stability in long-term studies. Advancements in manufacturing methods have resulted in a new class of serum with the potential to solve these challenges. An IFNɣ Elispot study was designed by the External Quality Assurance Program Oversight Laboratory (EQAPOL) at Duke Human Vaccine Institute's (DHVI) Immunology and Virology Quality Assessment Center (IVQAC) to test this new class of serum against their in-house, validated control serum, which is regarded as a global standard in performance for high functionality, recovery, and viability. Commonly used serum-free media were also included in the study. The results of this study compellingly demonstrate that this new class of serum produces high responses and low background reactivity comparable to the included serum standard, with excellent recovery and viability of cells. A protocol for ongoing testing has been developed to continuously validate new batches of this serum with the goal to make available to the field a pretested and validated serum that can be used with confidence in functional cell-based assays.
    MeSH term(s) Humans ; Reproducibility of Results ; Enzyme-Linked Immunospot Assay ; Laboratories ; Reference Standards
    Language English
    Publishing date 2023-02-28
    Publishing country Netherlands
    Document type Journal Article ; Review ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
    ZDB-ID 120142-6
    ISSN 1872-7905 ; 0022-1759
    ISSN (online) 1872-7905
    ISSN 0022-1759
    DOI 10.1016/j.jim.2023.113452
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 795: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (1.OG)
    ab Jg. 2022: Lesesaal (EG)

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  6. Article: Enhancing innovation-based urban competitiveness

    Ferrari, Guido

    Urban competitiveness and innovation , p. 53-66

    2014  , Page(s) 53–66

    Author's details Guido Ferrari
    Keywords Stadt ; Wettbewerb ; Innovation ; Allgemeines Gleichgewicht ; EU-Staaten
    Language English
    Publisher Elgar
    Publishing place Cheltenham [u.a.]
    Document type Article
    ISBN 978-1-78100-791-4 ; 1-78100-791-8
    Database ECONomics Information System

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  7. Article: CES and Translog parameter estimates in a SAM-based regional general equilibrium framework

    Ferrari, Guido / Secondi, Luca

    Economic systems research : journal of the International Input-Output Association Vol. 29, No. 1 , p. 124-141

    2017  Volume 29, Issue 1, Page(s) 124–141

    Author's details Guido Ferrari and Luca Secondi
    Keywords Regional CGE models ; SAM ; GCE estimation ; CES ; Translog
    Language English
    Publisher Routledge, Taylor & Francis Group
    Publishing place Abingdon
    Document type Article
    ZDB-ID 1007408-9 ; 2022495-3
    ISSN 1469-5758 ; 0953-5314
    ISSN (online) 1469-5758
    ISSN 0953-5314
    Database ECONomics Information System

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  8. Article ; Online: Single-cell analysis of immune cell transcriptome during HIV-1 infection and therapy.

    Pollara, Justin / Khanal, Santosh / Edwards, R Whitney / Hora, Bhavna / Ferrari, Guido / Haynes, Barton F / Bradley, Todd

    BMC immunology

    2022  Volume 23, Issue 1, Page(s) 48

    Abstract: Background: Cellular immune responses are phenotypically and functionally perturbed during HIV-1 infection, with the majority of function restored upon antiretroviral therapy (ART). Despite ART, residual inflammation remains that can lead to HIV-related ...

    Abstract Background: Cellular immune responses are phenotypically and functionally perturbed during HIV-1 infection, with the majority of function restored upon antiretroviral therapy (ART). Despite ART, residual inflammation remains that can lead to HIV-related co-morbidities and mortality, indicating that ART does not fully restore normal immune cell function. Thus, understanding the dynamics of the immune cell landscape during HIV-1 infection and ART is critical to defining cellular dysfunction that occurs during HIV-1 infection and imprints during therapy.
    Results: Here, we have applied single-cell transcriptome sequencing of peripheral blood immune cells from chronic untreated HIV-1 individuals, HIV-1-infected individuals receiving ART and HIV-1 negative individuals. We also applied single-cell transcriptome sequencing to a primary cell model of early HIV-1 infection using CD4+ T cells from healthy donors. We described changes in the transcriptome at high resolution that occurred during HIV-1 infection, and perturbations that remained during ART. We also determined transcriptional differences among T cells expressing HIV-1 transcripts that identified key regulators of HIV-1 infection that may serve as targets for future therapies to block HIV-1 infection.
    Conclusions: This work identified key molecular pathways that are altered in immune cells during chronic HIV-1 infection that could remain despite therapy. We also identified key genes that are upregulated during early HIV-1 infection that provide insights on the mechanism of HIV-1 infection and could be targets for future therapy.
    MeSH term(s) CD4-Positive T-Lymphocytes ; Graft vs Host Disease ; HIV Infections/drug therapy ; HIV-1 ; Humans ; Single-Cell Analysis ; Transcriptome
    Language English
    Publishing date 2022-09-29
    Publishing country England
    Document type Journal Article ; Research Support, N.I.H., Extramural
    ZDB-ID 2041500-X
    ISSN 1471-2172 ; 1471-2172
    ISSN (online) 1471-2172
    ISSN 1471-2172
    DOI 10.1186/s12865-022-00523-2
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  9. Article: Tourists’ Expenditure in Tuscany and its impact on the regional economic system

    Ferrari, Guido / José Mondéjar Jiménez / Luca Secondi

    Journal of cleaner production. 2018 Jan. 10, v. 171

    2018  

    Abstract: In this paper, we analyse the effects of tourists' demand on the economic system of Tuscany using a multiplier model based on the (64x64) regional Social Accounting Matrix 2011. To this end, after identifying the exogenous and endogenous accounts ( ... ...

    Abstract In this paper, we analyse the effects of tourists' demand on the economic system of Tuscany using a multiplier model based on the (64x64) regional Social Accounting Matrix 2011. To this end, after identifying the exogenous and endogenous accounts (variables), we obtain endogenous account coefficients and impact multipliers. Evidence exists that tourism activity is well integrated into the regional economic system. Moreover, this activity has a remarkable impact on food production, value added, and households' expenditure. A policy simulation shows that a positive shock in tourists’ expenditure results in an overall positive increase in demand for agriculture and industry products, as well as in an increase in regional value added, and institutional sector activity.
    Keywords accounting ; economic systems ; food production ; households ; issues and policy ; models ; multipliers ; tourism ; tourists ; value added ; Italy
    Language English
    Dates of publication 2018-0110
    Size p. 1437-1446.
    Publishing place Elsevier Ltd
    Document type Article
    ISSN 0959-6526
    DOI 10.1016/j.jclepro.2017.10.121
    Database NAL-Catalogue (AGRICOLA)

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  10. Article ; Online: A remarkable genetic shift in a transmitted/founder virus broadens antibody responses against HIV-1.

    Jain, Swati / Uritskiy, Gherman / Mahalingam, Marthandan / Batra, Himanshu / Chand, Subhash / Trinh, Hung V / Beck, Charles / Shin, Woong-Hee / Alsalmi, Wadad / Kijak, Gustavo / Eller, Leigh A / Kim, Jerome / Kihara, Daisuke / Tovanabutra, Sodsai / Ferrari, Guido / Robb, Merlin L / Rao, Mangala / Rao, Venigalla B

    eLife

    2024  Volume 13

    Abstract: A productive HIV-1 infection in humans is often established by transmission and propagation of a single transmitted/founder (T/F) virus, which then evolves into a complex mixture of variants during the lifetime of infection. An effective HIV-1 vaccine ... ...

    Abstract A productive HIV-1 infection in humans is often established by transmission and propagation of a single transmitted/founder (T/F) virus, which then evolves into a complex mixture of variants during the lifetime of infection. An effective HIV-1 vaccine should elicit broad immune responses in order to block the entry of diverse T/F viruses. Currently, no such vaccine exists. An in-depth study of escape variants emerging under host immune pressure during very early stages of infection might provide insights into such a HIV-1 vaccine design. Here, in a rare longitudinal study involving HIV-1 infected individuals just days after infection in the absence of antiretroviral therapy, we discovered a remarkable genetic shift that resulted in near complete disappearance of the original T/F virus and appearance of a variant with H173Y mutation in the variable V2 domain of the HIV-1 envelope protein. This coincided with the disappearance of the first wave of strictly H173-specific antibodies and emergence of a second wave of Y173-specific antibodies with increased breadth. Structural analyses indicated conformational dynamism of the envelope protein which likely allowed selection of escape variants with a conformational switch in the V2 domain from an α-helix (H173) to a β-strand (Y173) and induction of broadly reactive antibody responses. This differential breadth due to a single mutational change was also recapitulated in a mouse model. Rationally designed combinatorial libraries containing 54 conformational variants of V2 domain around position 173 further demonstrated increased breadth of antibody responses elicited to diverse HIV-1 envelope proteins. These results offer new insights into designing broadly effective HIV-1 vaccines.
    MeSH term(s) Animals ; Mice ; Humans ; HIV-1/genetics ; Antibody Formation ; Longitudinal Studies ; AIDS Vaccines/genetics ; Antibodies ; Antigens, Viral ; Dermatitis
    Chemical Substances AIDS Vaccines ; Antibodies ; Antigens, Viral
    Language English
    Publishing date 2024-04-15
    Publishing country England
    Document type Journal Article
    ZDB-ID 2687154-3
    ISSN 2050-084X ; 2050-084X
    ISSN (online) 2050-084X
    ISSN 2050-084X
    DOI 10.7554/eLife.92379
    Database MEDical Literature Analysis and Retrieval System OnLINE

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