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  1. Article ; Online: Inhibiting miR-195-5p Induces Proliferation of Human Corneal Endothelial Cells.

    Parekh, Mohit / Ramos, Tiago / Ferrari, Stefano / Ahmad, Sajjad

    International journal of molecular sciences

    2023  Volume 24, Issue 14

    Abstract: Transparency of the human cornea is responsible for clear vision, which is maintained by a monolayer of non-proliferative human corneal endothelial cells (HCEnCs). Dysfunction of these cells can result in irreversible corneal blindness. It is important ... ...

    Abstract Transparency of the human cornea is responsible for clear vision, which is maintained by a monolayer of non-proliferative human corneal endothelial cells (HCEnCs). Dysfunction of these cells can result in irreversible corneal blindness. It is important to identify key factors that limit the proliferation of HCEnCs and thus attempt to reverse them. Extracellular vesicles contain cargo which includes microRNAs (miRNAs) that can modulate a cellular function. In non small cell lung cancer, expression of miR-195-5p has been shown to inhibit proliferation; therefore, we aimed to investigate the inhibitory effect of miR-195-5p in inducing the proliferation of HCEnCs. Human corneal endothelial cell line (HCEC-12) and primary HCEnCs were cultured with miR-195-5p scramble, mimic or inhibitor. Corneal tissues from human cadaveric and FECD donors, and from pigs, mice and rabbits, were used for RT-PCR. miR-195-5p showed an abundance value of 11,363.31 a.u. When normalized against HCEnCs from cadaveric donors, FECD tissues showed a significant upregulation of miR-195-5p (
    MeSH term(s) Humans ; Animals ; Mice ; Rabbits ; Swine ; Endothelial Cells/metabolism ; Carcinoma, Non-Small-Cell Lung ; Lung Neoplasms ; MicroRNAs/genetics ; MicroRNAs/metabolism ; Cell Proliferation ; Cadaver
    Chemical Substances MicroRNAs ; MIRN195 microRNA, human
    Language English
    Publishing date 2023-07-15
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2019364-6
    ISSN 1422-0067 ; 1422-0067 ; 1661-6596
    ISSN (online) 1422-0067
    ISSN 1422-0067 ; 1661-6596
    DOI 10.3390/ijms241411490
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  2. Article: Effect of Low-Temperature Preservation in Optisol-GS on Preloaded, Endothelium-Out DMEK Grafts.

    Ruzza, Alessandro / Ferrari, Stefano / Airaldi, Matteo / Romano, Vito / Ponzin, Diego

    Journal of clinical medicine

    2023  Volume 12, Issue 3

    Abstract: The aim of the study was to assess different temperature ranges for the preservation of pre-loaded Descemet Membrane Endothelial Keratoplasty (DMEK) grafts in the DMEK RAPID Mini device.: Methods: Three groups of 15 DMEK grafts (five per group) were ... ...

    Abstract The aim of the study was to assess different temperature ranges for the preservation of pre-loaded Descemet Membrane Endothelial Keratoplasty (DMEK) grafts in the DMEK RAPID Mini device.
    Methods: Three groups of 15 DMEK grafts (five per group) were pre-loaded in the DMEK RAPID Mini and preserved in Optisol-GS for 72 h at different temperatures: group A at >8 °C, group B between 2-8 °C and group C at <2 °C. After stripping and preservation, the viability of the endothelium, cell loss and morphology were assessed through light microscopy following trypan blue and alizarin red staining.
    Results: Overall mortality was 4.07%, 3.97% and 7.66%, in groups A, B and C, respectively, with percentages of uncovered areas of 0.31%, 1.36% and 0.20% (all
    Conclusions: In the DMEK RAPID Mini, low temperatures (<2 °C) may affect the quality of pre-loaded grafts, inducing a higher ECL after 72 h of preservation, although no significant differences among groups could be proved. Our data would suggest maintaining grafts loaded in the DMEK RAPID Mini at temperatures between 2-8 °C for appropriate preservation.
    Language English
    Publishing date 2023-01-28
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2662592-1
    ISSN 2077-0383
    ISSN 2077-0383
    DOI 10.3390/jcm12031026
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  3. Article ; Online: Innovative Therapeutic Approaches for the Treatment of the Ocular Morbidities in Patients with EEC Syndrome.

    Barbaro, Vanessa / Bonelli, Filippo / Ferrari, Stefano / La Vella, Giulia / Di Iorio, Enzo

    Cells

    2023  Volume 12, Issue 3

    Abstract: Ectrodactyly-Ectodermal dysplasia-Clefting (EEC) syndrome is caused by heterozygous missense point mutations in ... ...

    Abstract Ectrodactyly-Ectodermal dysplasia-Clefting (EEC) syndrome is caused by heterozygous missense point mutations in the
    MeSH term(s) Humans ; Cleft Palate/genetics ; Cleft Lip/genetics ; Cleft Lip/therapy ; Ectodermal Dysplasia/genetics ; Ectodermal Dysplasia/therapy ; Ectodermal Dysplasia/diagnosis ; Transcription Factors/metabolism
    Chemical Substances Transcription Factors
    Language English
    Publishing date 2023-02-02
    Publishing country Switzerland
    Document type Journal Article ; Review ; Research Support, Non-U.S. Gov't
    ZDB-ID 2661518-6
    ISSN 2073-4409 ; 2073-4409
    ISSN (online) 2073-4409
    ISSN 2073-4409
    DOI 10.3390/cells12030495
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  4. Article ; Online: Comment on: A novel device to visualize Descemet membrane during donor preparation for Descemet membrane endothelial keratoplasty.

    Parekh, Mohit / Wongvisavavit, Rintra / Ferrari, Stefano / Romano, Vito

    Indian journal of ophthalmology

    2021  Volume 70, Issue 1, Page(s) 335–336

    MeSH term(s) Descemet Membrane/surgery ; Descemet Stripping Endothelial Keratoplasty ; Endothelium, Corneal ; Humans ; Tissue Donors
    Language English
    Publishing date 2021-12-22
    Publishing country India
    Document type Letter ; Comment
    ZDB-ID 187392-1
    ISSN 1998-3689 ; 0301-4738
    ISSN (online) 1998-3689
    ISSN 0301-4738
    DOI 10.4103/ijo.IJO_1777_21
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    In stock of ZB MED Cologne/Königswinter

    Zs.B 329: Show issues Location:
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  5. Article: Editorial: Corneal transplantation and eye banking.

    Romano, Vito / Ferrari, Stefano / Levis, Hannah J / Parekh, Mohit

    Frontiers in medicine

    2022  Volume 9, Page(s) 983580

    Language English
    Publishing date 2022-07-21
    Publishing country Switzerland
    Document type Editorial
    ZDB-ID 2775999-4
    ISSN 2296-858X
    ISSN 2296-858X
    DOI 10.3389/fmed.2022.983580
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  6. Article: Extracellular Vesicles Derived From Human Corneal Endothelial Cells Inhibit Proliferation of Human Corneal Endothelial Cells.

    Parekh, Mohit / Rhys, Hefin / Ramos, Tiago / Ferrari, Stefano / Ahmad, Sajjad

    Frontiers in medicine

    2022  Volume 8, Page(s) 753555

    Abstract: Corneal endothelial cells (CEnCs) are a monolayer of hexagonal cells that are responsible for maintaining the function and transparency of the cornea. Damage or dysfunction of CEnCs could lead to blindness. Human CEnCs (HCEnCs) have shown limited ... ...

    Abstract Corneal endothelial cells (CEnCs) are a monolayer of hexagonal cells that are responsible for maintaining the function and transparency of the cornea. Damage or dysfunction of CEnCs could lead to blindness. Human CEnCs (HCEnCs) have shown limited proliferative capacity
    Language English
    Publishing date 2022-02-04
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2775999-4
    ISSN 2296-858X
    ISSN 2296-858X
    DOI 10.3389/fmed.2021.753555
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  7. Article ; Online: Long-term preservation of human donor corneal tissues in organ culture.

    Parekh, Mohit / Ferrari, Stefano / Romano, Vito

    Cell and tissue banking

    2021  Volume 23, Issue 4, Page(s) 949–951

    MeSH term(s) Humans ; Organ Culture Techniques ; Cornea ; Corneal Transplantation ; Tissue Donors ; Organ Preservation ; Endothelium, Corneal ; Cell Count
    Language English
    Publishing date 2021-11-13
    Publishing country Netherlands
    Document type Journal Article
    ZDB-ID 2170897-6
    ISSN 1573-6814 ; 1389-9333
    ISSN (online) 1573-6814
    ISSN 1389-9333
    DOI 10.1007/s10561-021-09974-0
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    In stock of ZB MED Cologne/Königswinter

    Zs.A 5972: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
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  8. Article ; Online: Performance outcomes from a DMEK peeling and preparation wet lab.

    Parekh, Mohit / Wallace, Alexander George / Airaldi, Matteo / Ruzza, Alessandro / Ferrari, Stefano / Romano, Vito / Ahmad, Sajjad

    BMJ open ophthalmology

    2024  Volume 9, Issue 1

    Abstract: Objective: To evaluate the Descemet membrane endothelial keratoplasty (DMEK) preparation performance of trainee surgeons in an ex vivo human donor cornea DMEK wet lab simulation setting.: Methods: Human donor corneoscleral rims unsuitable for ... ...

    Abstract Objective: To evaluate the Descemet membrane endothelial keratoplasty (DMEK) preparation performance of trainee surgeons in an ex vivo human donor cornea DMEK wet lab simulation setting.
    Methods: Human donor corneoscleral rims unsuitable for transplantation were obtained from Moorfields Lions Eye Bank. At the wet lab, graft stripping was performed by scoring the peripheral endothelium. The trypan blue positive cells (TBPC) and cell density (cells/mm
    Results: Eight trainee surgeons attended the wet lab each attempting three DMEKs. Between the first and last attempts a significant decrease was seen in the procedural time (17.6 min vs 10.6 min (p<0.05)) and the TBPC % (12.9% vs 3.8% (p<0.05)). The percentage of tears peripherally and centrally also reduced between the first and the last trials (50% vs 13% (p=0.2226) and 38% vs 0% (p=0.1327)). A significant correlation was found between longer peeling times and higher TBPC % (p<0.001) with a 0.7% endothelial mortality increase for each additional minute required to complete the peel.
    Conclusions: DMEK wet labs provide a controlled risk-free learning opportunity for trainee surgeons to improve confidence and competence. Wet labs improve the success rate of DMEK graft preparation as well as flatten the learning curve. This emphasises the importance of continued support for the expansion of this valuable learning resource, promoting wider uptake of DMEK surgery.
    MeSH term(s) Humans ; Descemet Stripping Endothelial Keratoplasty ; Cornea/surgery ; Eye Banks ; Tissue Donors ; Learning Curve ; Trypan Blue
    Chemical Substances Trypan Blue (I2ZWO3LS3M)
    Language English
    Publishing date 2024-01-25
    Publishing country England
    Document type Journal Article
    ISSN 2397-3269
    ISSN (online) 2397-3269
    DOI 10.1136/bmjophth-2023-001540
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  9. Article ; Online: Assessment of performance and safety of Corneal Chamber hypothermic storage medium and PSS-L corneal rinsing solution in human and porcine corneas.

    Giurgola, Laura / Rodella, Umberto / Gatto, Claudio / Rossi, Orietta / Honisch, Claudia / Ragazzi, Eugenio / Ruzza, Paolo / Ferrari, Stefano / D'Amato Tóthová, Jana

    BMJ open ophthalmology

    2024  Volume 9, Issue 1

    Abstract: Purpose: To prove the safety and performance of the hypothermic corneal storage medium "Corneal Chamber" and the rinsing solution "PSS-L" in support of the new : Methods: Fifteen (n=15) human donor corneas and 11 (n=11) porcine corneas were evaluated ...

    Abstract Purpose: To prove the safety and performance of the hypothermic corneal storage medium "Corneal Chamber" and the rinsing solution "PSS-L" in support of the new
    Methods: Fifteen (n=15) human donor corneas and 11 (n=11) porcine corneas were evaluated for the following parameters: endothelial cell density (ECD) and mortality, percentage of hexagonal cells (HEX%), coefficient of cellular area variation (CV%) and corneal transparency at Day 0 and after 14±1 days of storage in Corneal Chamber medium at 2-8°C. Then, the same parameters were assessed after rinsing of corneas in PSS-L for 1 min at room temperature. Evaluation of gentamicin sulfate carryover after corneal storage and PSS-L rinsing was performed by ultra-high performance liquid chromatography analysis on human corneas homogenates.
    Results: Human and porcine corneas stored in Corneal Chamber medium showed a good overall quality of the tissue according to the quality parameters evaluated. In particular, mean ECD, HEX% and CV% did not show statistically significant changes at the end of storage and endothelial mortality increased to 3.1±3.3 and 7.8±3.5% in human and porcine corneas, respectively. Tissue rinsing with PSS-L did not affect the quality parameters evaluated before and gentamicin sulfate residues were absent in human corneas.
    Conclusions: Corneal preservation in Corneal Chamber medium at 2-8°C for 14 days and the corneal rinse with PSS-L are safe and effective procedures allowing the preservation of the corneal quality parameters as well as the complete elimination of gentamicin sulfate from the tissues before transplantation.Cite Now.
    MeSH term(s) Humans ; Swine ; Animals ; Endothelium, Corneal ; Cornea ; Organ Preservation/methods ; Corneal Transplantation/methods ; Gentamicins/pharmacology
    Chemical Substances Gentamicins
    Language English
    Publishing date 2024-02-22
    Publishing country England
    Document type Journal Article
    ISSN 2397-3269
    ISSN (online) 2397-3269
    DOI 10.1136/bmjophth-2023-001453
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  10. Article ; Online: Reproductive outcomes after vaginal repair of isthmocele: A preliminary study and systematic review of the literature.

    Candiani, Massimo / Dolci, Carolina / Schimberni, Matteo / Bartiromo, Ludovica / Villanacci, Roberta / Grisafi, Giorgia / Tandoi, Iacopo / Salvatore, Stefano / Ferrari, Stefano Maria

    European journal of obstetrics, gynecology, and reproductive biology

    2024  Volume 296, Page(s) 163–169

    Abstract: Objective: Although vaginal repair of isthmocele is an effective and safe surgical option, data on reproductive and obstetrical outcomes are lacking. The aim of this study is to evaluate reproductive outcomes of women undergone vaginal repair of ... ...

    Abstract Objective: Although vaginal repair of isthmocele is an effective and safe surgical option, data on reproductive and obstetrical outcomes are lacking. The aim of this study is to evaluate reproductive outcomes of women undergone vaginal repair of isthmocele. We also systematically reviewed the existent literature to offer a general view of available data.
    Study design: Retrospective analysis of a database prospectively collected between January 2018 and January 2022 at San Raffaele Hospital, Milan, Italy. We included secondary infertile women with ultrasound documented isthmocele who undergone vaginal repair. Post-surgical clinical, reproductive and obstetric outcomes were recorded. An advanced systematic search of the literature up to January 2023 was conducted.
    Results: 17 women were included. The mean age of the included patients was 37.2 ± 2.7 years. The median of previous caesarian sections was 1 (1-2). One intra-operative complication (5.9 %) was reported (bladder injury, repaired at the time of surgery). At follow up, bleeding was successfully treated in 8 women (8/10; 80 %). Pregnancy was obtained in 7 women (7/17; 41.2 %): the conception was spontaneous in 4 women (4/7; 57.1 %) and trough assisted reproductive technology in 3 patients (3/7; 42.9 %). The mean time from surgery to pregnancy was 10.8 (±6.7) months. One spontaneous abortion was reported (1/7; 14.3 %), while live birth was achieved in 6 pregnancies (6/7; 85.7 %). All deliveries were by caesarian section at a median gestational age of 37.5 (36-38.25) weeks. No obstetrical complications were reported. At the time of caesarean section, no defects on the lower segment were retrieved. Regarding the systematic research, among the 21 studies screened, only 4 articles were included in the review. Pregnancy rate was around 60-70 % with very few obstetrical complications (0.01 %) such as abnormal placentation or preterm birth.
    Conclusions: Vaginal repair of isthmocele is a minimally invasive, safe and effective surgical approach in terms of postsurgical residual myometrium tichness. Systematic review to date has found low-quality evidences on the impact of vaginal surgery in the management of secondary infertility and obstetrics outcomes in women with isthmocele.
    MeSH term(s) Infant, Newborn ; Pregnancy ; Humans ; Female ; Adult ; Infant ; Cesarean Section/adverse effects ; Infertility, Female/etiology ; Retrospective Studies ; Cicatrix/etiology ; Premature Birth
    Language English
    Publishing date 2024-02-13
    Publishing country Ireland
    Document type Systematic Review ; Journal Article ; Review
    ZDB-ID 190605-7
    ISSN 1872-7654 ; 0301-2115 ; 0028-2243
    ISSN (online) 1872-7654
    ISSN 0301-2115 ; 0028-2243
    DOI 10.1016/j.ejogrb.2024.02.025
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    In stock of ZB MED Cologne/Königswinter

    Zs.A 837: Show issues Location:
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