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Article ; Online: Measuring Protein-Protein Interactions and Quantifying Their Dissociation Constants with Mass Photometry.

Kofinova, Zornitsa / Karunanithy, Gogulan / Ferreira, Ana Sofia / Struwe, Weston B

Current protocols

2023 Volume 4, Issue 1, Page(s) e962

Abstract: Protein-protein interactions underlie most biological processes, and determining the affinity and abundance of binding partners for each interaction is often a challenging task because these interactions often involve multiple ligands and binding sites. ... ...

Abstract	Protein-protein interactions underlie most biological processes, and determining the affinity and abundance of binding partners for each interaction is often a challenging task because these interactions often involve multiple ligands and binding sites. Standard methods for determining the affinity of protein interactions often require a large amount of starting material in addition to potentially disruptive labeling or immobilization of the binding partners. Mass photometry is a bioanalytical technique that measures the mass of single biomolecules in solution, quickly and with minimal sample requirements. This article describes how mass photometry can be used to determine the mass distribution of binding partners, the complexes they form, the relative abundance of each species, and, accordingly, the dissociation constant (K
MeSH term(s)	Protein Binding ; Binding Sites ; Photometry
Language	English
Publishing date	2023-12-29
Publishing country	United States
Document type	Journal Article
ISSN	2691-1299
ISSN (online)	2691-1299
DOI	10.1002/cpz1.962
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Article ; Online: Natural Products for the Prevention and Treatment of Oral Mucositis-A Review.

Ferreira, Ana Sofia / Macedo, Catarina / Silva, Ana Margarida / Delerue-Matos, Cristina / Costa, Paulo / Rodrigues, Francisca

International journal of molecular sciences

2022 Volume 23, Issue 8

Abstract: Cancer, a major world public health problem, is associated with chemotherapy treatments whose administration leads to secondary concerns, such as oral mucositis (OM). The OM disorder is characterized by the presence of ulcers in the oral mucosa that ... ...

Abstract	Cancer, a major world public health problem, is associated with chemotherapy treatments whose administration leads to secondary concerns, such as oral mucositis (OM). The OM disorder is characterized by the presence of ulcers in the oral mucosa that cause pain, bleeding, and difficulty in ingesting fluids and solids, or speaking. Bioactive compounds from natural sources have arisen as an effective approach for OM. This review aims to summarize the new potential application of different natural products in the prevention and treatment of OM in comparison to conventional ones, also providing a deep insight into the most recent clinical studies. Natural products, such as
MeSH term(s)	Aloe/chemistry ; Animals ; Biological Products/pharmacology ; Biological Products/therapeutic use ; Mouth Mucosa ; Neoplasms/drug therapy ; Stomatitis/drug therapy ; Stomatitis/etiology ; Stomatitis/prevention & control
Chemical Substances	Biological Products
Language	English
Publishing date	2022-04-15
Publishing country	Switzerland
Document type	Journal Article ; Review
ZDB-ID	2019364-6
ISSN	1422-0067 ; 1422-0067 ; 1661-6596
ISSN (online)	1422-0067
ISSN	1422-0067 ; 1661-6596
DOI	10.3390/ijms23084385
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Article ; Online: The Post-Dictatorship Memory Politics in Portugal Which Erased Political Violence from the Collective Memory.

da Silva, Raquel / Ferreira, Ana Sofia

Integrative psychological & behavioral science

2018 Volume 53, Issue 1, Page(s) 24–43

Abstract: Former clandestine militants' voices and stories have been recurrently silenced in the Portuguese "battle over memory", because their activities were linked to events, such as the Revolution of 25 April 1974, which have themselves been politically and ... ...

Abstract	Former clandestine militants' voices and stories have been recurrently silenced in the Portuguese "battle over memory", because their activities were linked to events, such as the Revolution of 25 April 1974, which have themselves been politically and socially depreciated in mainstream political narratives. Only recently did the traditional political narratives start to be questioned and debated by Portuguese scholars. Such political narratives took root in the country in the decades that followed the April Revolution, with various scholars and politicians denying the fascist categorisation of Estado Novo and adopting an authoritarian, non-totalitarian and non-fascist perspective, while recurrently depicting the Revolution as highly negative (namely as the source of the economic troubles of the country). Thus, for a long time, Portuguese conservatives opted to avoid debates on the 48 years of the Estado Novo's regime which, among other things, maintained a very repressive and violent political police force, a camp of forced labour in Cape Vert known as Tarrafal, and a Colonial War on three African fronts. This article examines the existent academic publications which counter such oblivion of memory regarding armed struggle in Portugal. It also explores the reasons behind, on the one hand, the whitewashing of Estado Novo and the historical revisionism typical of the 1970s and 1980s, and, on the other hand, the "rebellion of memory" which emerged in the 1990s.
MeSH term(s)	History, 20th Century ; Humans ; Memory ; Politics ; Portugal ; Social Sciences ; Violence
Language	English
Publishing date	2018-07-24
Publishing country	United States
Document type	Historical Article ; Journal Article
ZDB-ID	2396310-4
ISSN	1936-3567 ; 1932-4502
ISSN (online)	1936-3567
ISSN	1932-4502
DOI	10.1007/s12124-018-9452-8
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Article ; Online: Impact on mortality of direct admission versus interhospital transfer in patients with ST-segment elevation myocardial infarction undergoing primary percutaneous coronary intervention.

Ferreira, Ana Sofia / Costa, João / Braga, Carlos Galvão / Marques, Jorge

Revista portuguesa de cardiologia

2019 Volume 38, Issue 9, Page(s) 621–631

Abstract: Introduction: In a primary percutaneous coronary intervention (PCI) program, interhospital transfer of patients with ST-elevation myocardial infarction (STEMI) can increase ischemic time, compared to patients who are admitted directly to a ... ...

Title translation	Impacto na mortalidade da admissão direta versus transferência inter‐hospitalar nos doentes com enfarte agudo do miocárdio com elevação do segmento ST submetidos a intervenção coronária percutânea primária.
Abstract	Introduction: In a primary percutaneous coronary intervention (PCI) program, interhospital transfer of patients with ST-elevation myocardial infarction (STEMI) can increase ischemic time, compared to patients who are admitted directly to a catheterization laboratory. Objectives: To assess the impact of interhospital transfer in patients with STEMI undergoing primary PCI, in terms of time to reperfusion and one-year mortality. Methods: This was an observational, retrospective, longitudinal study of patients with STEMI admitted to Hospital de Braga between June 2011 and May 2016, who were treated successfully within 12 hours of symptom onset. A total of 1222 patients were included and divided into two groups according to admission to Hospital de Braga: direct or interhospital transfer. Results: In this study, 37.0% (n=452) of the population were admitted directly to Hospital de Braga and 63.0% (n=770) were transferred from other hospitals. Although timings (in min) until reperfusion were longer in interhospital transfer patients (symptom onset-first medical contact (median 76.5, IQR 40.3-150 vs. 91.0, IQR 50-180, p=0.002), first medical contact-reperfusion (median 87.5, IQR 69.0-114 vs. 145, IQR 115-199, p<0.001) and symptom onset-reperfusion (median 177, IQR 125-265 vs. 265, IQR 188-400, p<0.001)), one-year mortality did not differ significantly between the groups (53 [11.7%] vs. 71 [9.2%], p=0.193). In multivariate analysis, age, symptom onset-reperfusion time and especially Killip class IV at admission (HR 11.2, 95% CI 6.35-19.8, p<0.001) were the main independent predictors of one-year mortality. Conclusion: Interhospital transfer of patients with STEMI increased the time before PCI. No differences were detected between groups in one-year mortality. This may be related to the fact that the direct admission group had twice as many patients in Killip class IV as the interhospital transfer group.
MeSH term(s)	Aged ; Aged, 80 and over ; Female ; Hospitalization/statistics & numerical data ; Humans ; Male ; Middle Aged ; Patient Transfer/statistics & numerical data ; Percutaneous Coronary Intervention/mortality ; Percutaneous Coronary Intervention/statistics & numerical data ; Retrospective Studies ; ST Elevation Myocardial Infarction/epidemiology ; ST Elevation Myocardial Infarction/mortality ; ST Elevation Myocardial Infarction/surgery
Language	Portuguese
Publishing date	2019-11-26
Publishing country	Spain
Document type	Journal Article
ZDB-ID	2646972-8
ISSN	2174-2049 ; 2174-2049
ISSN (online)	2174-2049
ISSN	2174-2049
DOI	10.1016/j.repc.2019.02.005
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Article ; Online: Insights into the polyphenols extraction from Actinidia arguta fruit (kiwiberry): A source of pro-healthy compounds

Macedo, Catarina / Silva, Ana Margarida / Ferreira, Ana Sofia / Cádiz-Gurrea, María de la Luz / Fernández-Ochoa, Álvaro / Segura-Carretero, Antonio / Delerue-Matos, Cristina / Costa, Paulo / Rodrigues, Francisca

Scientia Horticulturae. 2023 Apr., v. 313 p.111910-

2023

Abstract: Actinidia arguta fruit (also known as kiwiberry) has a remarkable phytochemical profile, being reported as a promising preventive agent/treatment for chronic diseases and a desirable source of bioactive molecules for pharmaceutical and cosmetic ... ...

Abstract	Actinidia arguta fruit (also known as kiwiberry) has a remarkable phytochemical profile, being reported as a promising preventive agent/treatment for chronic diseases and a desirable source of bioactive molecules for pharmaceutical and cosmetic industries. Despite these promising indications, investigating the full potential of A. arguta fruit remains an unmet need for health applications. This work aims to determine the optimal extraction conditions of antioxidant/antiradical compounds from A. arguta fruit (commonly known as kiwiberry), using the Response Surface Methodology (RSM). The effects of probe amplitude (30%-70%), sonication time (5-30 min) and water:ethanol solvent ratio (0%-100%) were assessed. The optimal extraction conditions were achieved using 50% of water, during 17.5 min and using an amplitude of 50%. A total of 22 compounds, including 6 flavonoids and 4 phenolic acids, were identified in the optimal extract through HPLC-ESI-QTOF-MS, which exhibited outstanding antioxidant and antiradical activities (TPC = 18.705 mg GAE/g dw; FRAP = 186.876 µmol FSE/g dw; ABTS = 16.334 mg AAE/g dw; O₂·⁻ IC₅₀ = 829.384 µg/mL; HOCl IC₅₀ = 16.895 µg/mL; ROO· = 0.18 µg TE/mg dw). Moreover, it displayed antimicrobial activity against Staphylococcus aureus (MIC = 32 mg/mL) and Pseudomonas gingivalis (MIC = 64 mg/mL) and reduced the growth rate of Escherichia coli. In-vitro assays demonstrated that the optimal extract successfully decreased the viability of two human carcinoma cell lines, namely TR146 and HSC-3, at 500 µg/mL and above 500 µg/mL, respectively. The present study showed that A. arguta fruit is a rich source of compounds with potential to be used for pro-healthy purposes.
Keywords	Actinidia arguta ; Escherichia coli ; Pseudomonas ; Staphylococcus aureus ; antimicrobial properties ; antioxidants ; chemical constituents of plants ; flavonoids ; fruits ; humans ; neoplasm cells ; polyphenols ; response surface methodology ; solvents ; sonication ; viability ; Ultrasound-assisted extraction ; Phenolic compounds ; Pro-healthy properties
Language	English
Dates of publication	2023-04
Publishing place	Elsevier B.V.
Document type	Article ; Online
Note	Use and reproduction
ZDB-ID	185557-8
ISSN	0304-4238
ISSN	0304-4238
DOI	10.1016/j.scienta.2023.111910
Database	NAL-Catalogue (AGRICOLA)

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Article ; Online: Revisiting Ionic Liquid Structure-Property Relationship: A Critical Analysis.

Silva, Wagner / Zanatta, Marcileia / Ferreira, Ana Sofia / Corvo, Marta C / Cabrita, Eurico J

International journal of molecular sciences

2020 Volume 21, Issue 20

Abstract: In the last few years, ionic liquids (ILs) have been the focus of extensive studies concerning the relationship between structure and properties and how this impacts their application. Despite a large number of studies, several topics remain ... ...

Abstract	In the last few years, ionic liquids (ILs) have been the focus of extensive studies concerning the relationship between structure and properties and how this impacts their application. Despite a large number of studies, several topics remain controversial or not fully answered, such as: the existence of ion pairs, the concept of free volume and the effect of water and its implications in the modulation of ILs physicochemical properties. In this paper, we present a critical review of state-of-the-art literature regarding structure-property relationship of ILs, we re-examine analytical theories on the structure-property correlations and present new perspectives based on the existing data. The interrelation between transport properties (viscosity, diffusion, conductivity) of IL structure and free volume are analysed and discussed at a molecular level. In addition, we demonstrate how the analysis of microscopic features (particularly using NMR-derived data) can be used to explain and predict macroscopic properties, reaching new perspectives on the properties and application of ILs.
MeSH term(s)	Diffusion ; Electric Conductivity ; Ionic Liquids/chemistry ; Ions ; Structure-Activity Relationship ; Viscosity
Chemical Substances	Ionic Liquids ; Ions
Language	English
Publishing date	2020-10-19
Publishing country	Switzerland
Document type	Journal Article ; Review
ZDB-ID	2019364-6
ISSN	1422-0067 ; 1422-0067 ; 1661-6596
ISSN (online)	1422-0067
ISSN	1422-0067 ; 1661-6596
DOI	10.3390/ijms21207745
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Article ; Online: Sex-specific changes in peripheral metabolism in a model of chronic anxiety induced by prenatal stress.

Ferreira, Ana Sofia / Galvão, Sofia / Gaspar, Rita / Rodrigues-Neves, Ana C / Ambrósio, António F / Matafome, Paulo / Gomes, Catarina A / Baptista, Filipa I

European journal of clinical investigation

2021 Volume 51, Issue 12, Page(s) e13639

Abstract: Background: Prenatal stress is associated with increased susceptibility to psychiatric and metabolic disorders later in life. Prenatal exposure to stress mediators may have sex-dependent effects on offspring brain and metabolic function, promoting a sex- ...

Abstract	Background: Prenatal stress is associated with increased susceptibility to psychiatric and metabolic disorders later in life. Prenatal exposure to stress mediators may have sex-dependent effects on offspring brain and metabolic function, promoting a sex-specific vulnerability to psychopathology and metabolic alterations at adulthood. In this work, the impact of prenatal stress on glucose homeostasis and peripheral metabolism of male and female offspring was investigated in a chronic anxiety animal model. Methods: Pregnant Wistar rats were injected with saline or glucocorticoid (dexamethasone: 1 mg/kg, subcutaneous) at gestational days 18 and 19. Male and female offspring weight was monitored, and anxious-like behaviour and peripheral insulin-sensitive tissues were analysed at adulthood. Results: At birth, females and males prenatally exposed to stress presented decreased body weight which remained low in females. At adulthood, a morphological disorganization of the Langerhans islets was observed in both sexes prenatally exposed to stress, yet not changes in insulin levels were detected. Also, prenatal stress increased glucose transporter 4 (GLUT-4) levels in female and male adipose tissues and decreased insulin receptor levels in the liver and skeleton muscle but only in females. Conclusions: Exposure to stress mediators in critical periods of development negatively affects behaviour and metabolism. Prenatal stress programmes offspring peripheral metabolism in a sex-specific manner, emphasizing that the response to stress in critical periods of development may be sex-specific having each sex different vulnerabilities to psychiatric and metabolic disorders. Considering sex-specificities may provide critical clues for the design of preventive strategies and for early therapeutic intervention.
MeSH term(s)	Adipose Tissue/metabolism ; Animals ; Anxiety/metabolism ; Disease Models, Animal ; Female ; Glucose/metabolism ; Glucose Transporter Type 4/metabolism ; Insulin/metabolism ; Islets of Langerhans/growth & development ; Islets of Langerhans/metabolism ; Islets of Langerhans/pathology ; Liver/metabolism ; Male ; Muscle, Skeletal/metabolism ; Pregnancy ; Pregnancy Complications/metabolism ; Prenatal Exposure Delayed Effects/metabolism ; Rats ; Receptor, Insulin/metabolism ; Sex Factors ; Stress, Psychological/metabolism
Chemical Substances	Glucose Transporter Type 4 ; Insulin ; Slc2a4 protein, rat ; Receptor, Insulin (EC 2.7.10.1) ; Glucose (IY9XDZ35W2)
Language	English
Publishing date	2021-06-22
Publishing country	England
Document type	Journal Article
ZDB-ID	186196-7
ISSN	1365-2362 ; 0014-2972 ; 0960-135X
ISSN (online)	1365-2362
ISSN	0014-2972 ; 0960-135X
DOI	10.1111/eci.13639
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Article ; Online: New Perspectives on the Sustainable Employment of Chestnut Shells as Active Ingredient against Oral Mucositis: A First Screening.

Ferreira, Ana Sofia / Silva, Ana Margarida / Pinto, Diana / Moreira, Manuela M / Ferraz, Ricardo / Švarc-Gajić, Jaroslava / Costa, Paulo C / Delerue-Matos, Cristina / Rodrigues, Francisca

International journal of molecular sciences

2022 Volume 23, Issue 23

Abstract: Oral mucositis (OM), a common side effect of oncological treatment, is an oral mucosal disorder characterized by painful ulcerations and increased risk of infection. The use of natural antioxidants to suppress the redox imbalance responsible for the OM ... ...

Abstract	Oral mucositis (OM), a common side effect of oncological treatment, is an oral mucosal disorder characterized by painful ulcerations and increased risk of infection. The use of natural antioxidants to suppress the redox imbalance responsible for the OM condition has emerged as an interesting approach to prevent/treat OM. This study aims to explore the chestnut (
Language	English
Publishing date	2022-11-29
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2019364-6
ISSN	1422-0067 ; 1422-0067 ; 1661-6596
ISSN (online)	1422-0067
ISSN	1422-0067 ; 1661-6596
DOI	10.3390/ijms232314956
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Article ; Online: Draft Genome Sequence of a Leptospira kirschneri Serovar Mozdok Type 2 Strain Isolated from a Horse in Portugal.

Inácio, João / Muñoz-Mérida, Antonio / Ahmed, Ahmed A / Rocha, Teresa / Mesquita, João Rodrigo / Thompson, Gertrude / Goris, Marga G A / Hartskeerl, Rudy A / Ferreira, Ana Sofia

Microbiology resource announcements

2021 Volume 10, Issue 28, Page(s) e0021721

Abstract: Leptospira kirschneri is an agent causing leptospirosis in animals and humans. We report the draft genome sequence of Leptospira kirschneri serovar Mozdok type 2 strain Horse 112, comprising 485 contigs and having a genome size of 4,301,784 bp. This ... ...

Abstract	Leptospira kirschneri is an agent causing leptospirosis in animals and humans. We report the draft genome sequence of Leptospira kirschneri serovar Mozdok type 2 strain Horse 112, comprising 485 contigs and having a genome size of 4,301,784 bp. This genome will facilitate studying important mechanisms for clinical outcomes.
Language	English
Publishing date	2021-07-15
Publishing country	United States
Document type	Journal Article
ISSN	2576-098X
ISSN (online)	2576-098X
DOI	10.1128/MRA.00217-21
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Article ; Online: SARS-CoV-2 and blood donations in Portugal, June-July 2020.

Mesquita, João R / Barradas, Patrícia / Gomes da Silva, Priscilla / Ferreira, Ana Sofia / Silva, Eliane / Matas, Isabel M / Thomson, Gertrude / Amorim, Irina / Duarte, Raquel / Gomes, Helena Cruz / Monteiro, Álvaro / Nascimento, Maria São José

Journal of medical virology

2021 Volume 94, Issue 1, Page(s) 42–43

MeSH term(s)	Blood Donors/statistics & numerical data ; Blood Transfusion/statistics & numerical data ; COVID-19/transmission ; Humans ; Immunoglobulin G/blood ; Immunoglobulin M/blood ; Portugal ; RNA, Viral/blood ; SARS-CoV-2/immunology ; SARS-CoV-2/isolation & purification ; Seroepidemiologic Studies
Chemical Substances	Immunoglobulin G ; Immunoglobulin M ; RNA, Viral
Language	English
Publishing date	2021-09-27
Publishing country	United States
Document type	Letter ; Research Support, Non-U.S. Gov't
ZDB-ID	752392-0
ISSN	1096-9071 ; 0146-6615
ISSN (online)	1096-9071
ISSN	0146-6615
DOI	10.1002/jmv.27353
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