LIVIVO - Search results -

Search results

Result 1 - 10 of total 10

Search options

Article ; Online: Time to Recurrence as a Prognostic Factor in Parathyroid Carcinoma.

Magnabosco, Felipe Ferraz / Brescia, Marilia D'Elboux Guimarães / Nascimento Júnior, Climério Pereira / Massoni Neto, Ledo Mazzei / Arap, Sergio Samir / de Castro Junior, Gilberto / Ledesma, Felipe Lourenço / Ferreira Alves, Venancio Avancini / Kowalski, Luiz Paulo / Martin, Regina Matsunaga / de Menezes Montenegro, Fabio Luiz

Journal of the Endocrine Society

2023 Volume 7, Issue 7, Page(s) bvad067

Abstract: Background: Parathyroid carcinoma (PC) is a rare and challenging disease without clearly understood prognostic factors. Adequate management can improve outcomes. Characteristics of patients treated for PC over time and factors affecting prognosis were ... ...

Abstract	Background: Parathyroid carcinoma (PC) is a rare and challenging disease without clearly understood prognostic factors. Adequate management can improve outcomes. Characteristics of patients treated for PC over time and factors affecting prognosis were analyzed. Methods: Retrospective cohort study including surgically treated patients for PC between 2000 and 2021. If malignancy was suspected, free-margin resection was performed. Demographic, clinical, laboratory, surgical, pathological, and follow-up characteristics were assessed. Results: Seventeen patients were included. Mean tumor size was 32.5 mm, with 64.7% staged as pT1/pT2. None had lymph node involvement at admission, and 2 had distant metastases. Parathyroidectomy with ipsilateral thyroidectomy was performed in 82.2%. Mean postoperative calcium levels were different between patients who developed recurrence vs those who did not ( Conclusion: Patients with PC can survive for decades and have indolent disease course. Free margins seem to be the most important factor in initial surgery. Recurrence was common (60%), but patients with disease recurrence within 36 months of initial surgery had a lower survival rate.
Language	English
Publishing date	2023-05-23
Publishing country	United States
Document type	Journal Article
ISSN	2472-1972
ISSN (online)	2472-1972
DOI	10.1210/jendso/bvad067
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Article ; Online: Understanding yellow fever-associated myocardial injury: an autopsy study.

Giugni, Fernando Rabioglio / Aiello, Vera Demarchi / Faria, Caroline Silverio / Pour, Shahab Zaki / Cunha, Marielton Dos Passos / Giugni, Melina Valdo / Pinesi, Henrique Trombini / Ledesma, Felipe Lourenço / Morais, Carolina Esteves / Ho, Yeh-Li / Sztajnbok, Jaques / de Morais Fernezlian, Sandra / Ferraz da Silva, Luiz Fernando / Mauad, Thais / Ferreira Alves, Venâncio Avancini / Hilário do Nascimento Saldiva, Paulo / Antonangelo, Leila / Dolhnikoff, Marisa / Duarte-Neto, Amaro Nunes

EBioMedicine

2023 Volume 96, Page(s) 104810

Abstract: Background: Yellow fever (YF) is a viral hemorrhagic fever, endemic in parts of South America and Africa. There is scarce evidence about the pathogenesis of the myocardial injury. The objective of this study is to evaluate the cardiac pathology in fatal ...

Abstract	Background: Yellow fever (YF) is a viral hemorrhagic fever, endemic in parts of South America and Africa. There is scarce evidence about the pathogenesis of the myocardial injury. The objective of this study is to evaluate the cardiac pathology in fatal cases of YF. Methods: This retrospective autopsy study included cases from the São Paulo (Brazil) epidemic of 2017-2019. We reviewed medical records and performed cardiac tissue histopathological evaluation, electron microscopy, immunohistochemical assays, RT-qPCR for YF virus (YFV)-RNA, and proteomics analysis on inflammatory and endothelial biomarkers. Findings: Seventy-three confirmed YF cases with a median age of 48 (34-60) years were included. We observed myocardial fibrosis in 68 (93.2%) patients; cardiomyocyte hypertrophy in 68 (93.2%); endothelial alterations in 67 (91.8%); fiber necrosis in 50 (68.5%); viral myocarditis in 9 (12.3%); and secondary myocarditis in 5 (6.8%). Four out of five patients with 17DD vaccine-associated viscerotropic disease presented with myocarditis. The cardiac conduction system showed edema, hemorrhages and endothelial fibrinoid necrosis. Immunohistochemistry detected CD68-positive inflammatory interstitial cells and YFV antigens in endothelial and inflammatory cells. YFV-RNA was detected positive in 95.7% of the cardiac samples. The proteomics analysis demonstrated that YF patients had higher levels of multiple inflammatory and endothelial biomarkers in comparison to cardiovascular controls, and higher levels of interferon gamma-induced protein 10 (IP-10) in comparison to sepsis (p = 0.01) and cardiovascular controls (p < 0.001) in Dunn test. Interpretation: Myocardial injury is frequent in severe YF, due to multifactorial mechanisms, including direct YFV-mediated damage, endothelial cell injury, and inflammatory response, with a possible prominent role for IP-10. Funding: This study was funded by Fundação de Amparo à Pesquisa do Estado de São Paulo, Bill and Melinda Gates Foundation, Conselho Nacional de Desenvolvimento Científico e Tecnológico, Coordenação de Aperfeiçoamento de Pessoal de Nível Superior.
MeSH term(s)	Humans ; Middle Aged ; Yellow Fever/epidemiology ; Myocarditis/etiology ; Chemokine CXCL10 ; Retrospective Studies ; Brazil/epidemiology ; Heart Injuries ; RNA ; Autopsy ; Biomarkers ; Necrosis
Chemical Substances	Chemokine CXCL10 ; RNA (63231-63-0) ; Biomarkers
Language	English
Publishing date	2023-09-25
Publishing country	Netherlands
Document type	Review ; Journal Article
ZDB-ID	2851331-9
ISSN	2352-3964
ISSN (online)	2352-3964
DOI	10.1016/j.ebiom.2023.104810
Database	MEDical Literature Analysis and Retrieval System OnLINE

In stock of ZB MED Cologne/Königswinter

Zs.A 7090: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular
ab Jg. 2022: Lesesaal (EG)

Order via subito

Details ▾
- See ZB MED holdings
- Order with fees

Article ; Online: The complement component C5 promotes liver steatosis and inflammation in murine non-alcoholic liver disease model.

Bavia, Lorena / Cogliati, Bruno / Dettoni, Juliano Bertollo / Ferreira Alves, Venancio Avancini / Isaac, Lourdes

Immunology letters

2016 Volume 177, Page(s) 53–61

Abstract: Non-Alcoholic Fatty Liver Disease (NALD) is considering a hepatic manifestation of metabolic syndrome. Although the pathogenesis of NALD is not completely understood, insulin resistance and inflammatory cytokines are implicated. Considering that ... ...

Abstract	Non-Alcoholic Fatty Liver Disease (NALD) is considering a hepatic manifestation of metabolic syndrome. Although the pathogenesis of NALD is not completely understood, insulin resistance and inflammatory cytokines are implicated. Considering that component C5 is a central mediator of inflammation, we investigated the role of C5 in the establishment of NALD. Eight to ten-week old B6 C5(+) and A/J C5(-) male mice were fed a high fat diet containing glucose (HFDG) for 6 and 10 weeks. We observed that B6 C5(+) mice HFDG-fed for 10 weeks developed hepatomegaly, triglycerides (TG) accumulation, steatosis and enhanced liver TNF-α, IL-6, IL-12p70 and IL-17 levels when compared to A/J C5(-) mice. Next, B6 C5(+) mice were compared with congenic B6 C5(-) mice. Again, B6 C5(+) HFDG-fed mice developed more steatosis, liver centro-lobular inflammation and presented higher levels of liver IL-1β, IL-12p70, IL-17 and TFG-β than B6 C5(-) mice under the same conditions. B6 C5(+) mice HFDG-fed also presented lower concentrations of serum albumin, serum cholesterol, blood leukocytes and liver NO production when compared with B6 C5(-) mice. We concluded that murine C5 contributes effectively to liver steatosis and inflammation in NALD pathogenesis. In addition, C5 is also important to control serum cholesterol and albumin levels in the C57BL/6 genetic background.
Language	English
Publishing date	2016-09
Publishing country	Netherlands
Document type	Journal Article
ZDB-ID	445150-8
ISSN	1879-0542 ; 0165-2478
ISSN (online)	1879-0542
ISSN	0165-2478
DOI	10.1016/j.imlet.2016.07.014
Database	MEDical Literature Analysis and Retrieval System OnLINE

In stock of ZB MED Cologne/Königswinter

Zs.A 1512: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
Jg. 1995 - 2021: Lesesall (1.OG)
ab Jg. 2022: Lesesaal (EG)

Order via subito

Details ▾
- See ZB MED holdings
- Order with fees

Article ; Online: Omega-3 polyunsaturated fatty acids in treating non-alcoholic steatohepatitis: A randomized, double-blind, placebo-controlled trial.

Nogueira, Monize Aydar / Oliveira, Claudia Pinto / Ferreira Alves, Venâncio Avancini / Stefano, José Tadeu / Rodrigues, Lívia Samara Dos Reis / Torrinhas, Raquel Susana / Cogliati, Bruno / Barbeiro, Hermes / Carrilho, Flair José / Waitzberg, Dan Linetzky

Clinical nutrition (Edinburgh, Scotland)

2016 Volume 35, Issue 3, Page(s) 578–586

Abstract: Background: & aims: Few clinical trials have addressed the potential benefits of omega-3 polyunsaturated fatty acids (PUFAs) on non-alcoholic steatohepatitis (NASH). We evaluated the effects of supplementation with omega-3 PUFAs from flaxseed and fish ... ...

Abstract	Background: & aims: Few clinical trials have addressed the potential benefits of omega-3 polyunsaturated fatty acids (PUFAs) on non-alcoholic steatohepatitis (NASH). We evaluated the effects of supplementation with omega-3 PUFAs from flaxseed and fish oils in patients with biopsy-proven NASH. Methods: Patients received three capsules daily, each containing 0.315 g of omega-3 PUFAs (64% alpha-linolenic [ALA], 16% eicosapentaenoic [EPA], and 21% docosahexaenoic [DHA] acids; n-3 group, n = 27) or mineral oil (placebo group, n = 23). Liver biopsies were evaluated histopathologically by the NASH activity score (NAS). Plasma levels of omega-3 PUFAs were assessed as a marker of intake at baseline and after 6 months of treatment. Secondary endpoints included changes in plasma biochemical markers of lipid metabolism, inflammation, and liver function at baseline and after 3 and 6 months of treatment. Results: At baseline, NAS was comparable between the groups (p = 0.98). After intervention with omega-3 PUFAs, plasma ALA and EPA levels increased (p ≤ 0.05). However in the placebo group, we also observed increased EPA and DHA (p ≤ 0.05), suggesting an off-protocol intake of PUFAs. NAS improvement/stabilization was correlated with increased ALA in the n-3 group (p = 0.02) and with increased EPA (p = 0.04) and DHA (p = 0.05) in the placebo group. Triglycerides were reduced after 3 months in the n-3 group compared to baseline (p = 0.01). Conclusions: In NASH patients, the supplementation of omega-3 PUFA from flaxseed and fish oils significantly impacts on plasma lipid profile of patients with NASH. Plasma increase of these PUFAs was associated with better liver histology. (ID 01992809).
Language	English
Publishing date	2016-06
Publishing country	England
Document type	Journal Article
ZDB-ID	604812-2
ISSN	1532-1983 ; 0261-5614
ISSN (online)	1532-1983
ISSN	0261-5614
DOI	10.1016/j.clnu.2015.05.001
Database	MEDical Literature Analysis and Retrieval System OnLINE

In stock of ZB MED Cologne/Königswinter

Zs.A 1774: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
Jg. 1995 - 2021: Lesesall (1.OG)
ab Jg. 2022: Lesesaal (EG)

Order via subito

Details ▾
- See ZB MED holdings
- Order with fees

Article: Identi.cation of the Primary Site of Metastatic Adenocarcinoma in Serous Effusions

Filho, Adhemar Longatto / Ferreira Alves, Venâncio Avancini / Takami Kanamura, Cristina / Nonogaki, Suely / Bortolan, Jeni / Lombardo, Valeria / Bisi, Helio

Acta Cytologica

2011 Volume 46, Issue 4, Page(s) 651–658

Institution	From the Pathology Division, Alfredo Lutz Institute; Pathology Department, São Paolo State University Medical School; Surgical Pathology Department, A. C. Camargo Hospital; and Division of Epidemiology and Biostatistics, Oncocentro Foundation, São Paolo, Brazil
Language	English
Publishing date	2011-03-04
Publisher	S. Karger AG
Publishing place	Basel, Switzerland
Document type	Article
Note	Original Articles
ZDB-ID	80003-x
ISSN	1938-2650 ; 0001-5547
ISSN (online)	1938-2650
ISSN	0001-5547
DOI	10.1159/000326970
Database	Karger publisher's database

In stock of ZB MED Cologne/Königswinter

Zs.A 358: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
Jg. 1995 - 2021: Lesesall (1.OG)
ab Jg. 2022: Lesesaal (EG)

Order via subito

Details ▾
- See ZB MED holdings
- Order with fees

Article: Contribution of immunohistochemistry to small B-cell lymphoma classification.

Coelho Siqueira, Sheila Aparecida / Ferreira Alves, Venancio Avancini / Beitler, Beatriz / Otta, Marta Mitiko / Nascimento Saldiva, Paulo Hilario

Applied immunohistochemistry & molecular morphology : AIMM

2006 Volume 14, Issue 1, Page(s) 1–6

Abstract: Although the small B-cell lymphomas show major morphologic overlapping, they have been recently shown to be distinct entities with several biologic and clinical differences. Therefore, the utility of a panel of paraffin-reactive antibodies in ... ...

Abstract	Although the small B-cell lymphomas show major morphologic overlapping, they have been recently shown to be distinct entities with several biologic and clinical differences. Therefore, the utility of a panel of paraffin-reactive antibodies in differentiating these neoplasms was investigated. Using clinical data and morphologic criteria, 134 cases of small B-cell lymphomas were grouped as those with (1) one strongly suggested diagnosis, (2) differential diagnosis between two types of lymphomas, and (3) small B-cell lymphoma without hints for further subclassification. With a panel of antibodies including CD5, CD10, CD23, CD43, bcl-2, and cyclin D1, most but not all cases could be precisely categorized. This panel confirmed the diagnosis in 96.5% of the cases from group 1. In group 2 it confirmed one of the two diagnoses in 81.5% of the cases. In group 3 it established a definitive diagnosis in 55% of the cases. When all groups were considered, a correct diagnosis could be established for 88.1% of cases; for 6.7% of them the authors remained with two possible diagnosis, and the broad "small B-cell lymphoma" was the only diagnosis for 5.2% of cases. CD10 separated most follicular lymphomas from other small B-cell lymphoid neoplasms. CD23 separated small lymphocytic lymphoma/chronic lymphocytic leukemia. Cyclin D1 separated mantle cell lymphoma. The present study selected CD10, CD23, and cyclin D1 as a minimal panel for the classification of small B-cell lymphomas, yielding a final diagnosis in 88.1% of the cases.
MeSH term(s)	Antigens, CD/biosynthesis ; B-Lymphocytes/immunology ; Diagnosis, Differential ; Humans ; Immunohistochemistry ; Immunophenotyping ; Leukemia, Lymphocytic, Chronic, B-Cell/classification ; Leukemia, Lymphocytic, Chronic, B-Cell/diagnosis ; Leukemia, Lymphocytic, Chronic, B-Cell/pathology ; Lymphoma, Non-Hodgkin/classification ; Lymphoma, Non-Hodgkin/diagnosis ; Lymphoma, Non-Hodgkin/pathology ; Proto-Oncogene Proteins c-bcl-2/biosynthesis
Chemical Substances	Antigens, CD ; Proto-Oncogene Proteins c-bcl-2
Language	English
Publishing date	2006-03
Publishing country	United States
Document type	Journal Article
ISSN	1541-2016
ISSN	1541-2016
DOI	10.1097/01.pai.0000153721.13531.c2
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Article ; Online: Association of polymorphisms of glutamate-cystein ligase and microsomal triglyceride transfer protein genes in non-alcoholic fatty liver disease.

Oliveira, Claudia Pinto Marques Souza / Stefano, José Tadeu / Cavaleiro, Ana Mercedes / Zanella Fortes, Maria Angela Henriques / Vieira, Suzana Maria / Rodrigues Lima, Vicência Mara / Santos, Telma Eugenio / Santos, Virginia Nascimento / de Azevedo Salgado, Ana Lucia Farias / Parise, Edson Roberto / Ferreira Alves, Venâncio Avancini / Carrilho, Flair José / Corrêa-Giannella, Maria Lucia

Journal of gastroenterology and hepatology

2010 Volume 25, Issue 2, Page(s) 357–361

Abstract: Background and aims: Although the metabolic risk factors for non-alcoholic fatty liver disease (NAFLD) progression have been recognized, the role of genetic susceptibility remains a field to be explored. The aim of this study was to examine the ... ...

Abstract	Background and aims: Although the metabolic risk factors for non-alcoholic fatty liver disease (NAFLD) progression have been recognized, the role of genetic susceptibility remains a field to be explored. The aim of this study was to examine the frequency of two polymorphisms in Brazilian patients with biopsy-proven simple steatosis or non-alcoholic steatohepatitis (NASH): -493 G/T in the MTP gene, which codes the protein responsible for transferring triglycerides to nascent apolipoprotein B, and -129 C/T in the GCLC gene, which codes the catalytic subunit of glutamate-cystein ligase in the formation of glutathione. Methods: One hundred and thirty-one biopsy-proven NAFLD patients (n = 45, simple steatosis; n = 86, NASH) and 141 unrelated healthy volunteers were evaluated. Genomic DNA was extracted from peripheral blood cells, and the -129 C/T polymorphism of the GCLC gene was determined by restriction fragment length polymorphism (RFLP). The -493 G/T polymorphism of the MTP gene was determined by direct sequencing of the polymerase chain reaction products. Results: The presence of at least one T allele in the -129 C/T polymorphism of the GCLC gene was independently associated with NASH (odds ratio 12.14, 95% confidence interval 2.01-73.35; P = 0.007), whereas, the presence of at least one G allele in the -493 G/T polymorphism of the MTP gene differed slightly between biopsy-proven NASH and simple steatosis. Conclusion: This difference clearly warrants further investigation in larger samples. These two polymorphisms could represent an additional factor for consideration in evaluating the risk of NAFLD progression. Further studies involving a larger population are necessary to confirm this notion.
MeSH term(s)	Biopsy ; Brazil ; Carrier Proteins/genetics ; Case-Control Studies ; Chi-Square Distribution ; Fatty Liver/enzymology ; Fatty Liver/genetics ; Fatty Liver/pathology ; Female ; Gene Frequency ; Genetic Predisposition to Disease ; Glutamate-Cysteine Ligase/genetics ; Humans ; Liver/enzymology ; Liver/pathology ; Male ; Middle Aged ; Odds Ratio ; Phenotype ; Polymerase Chain Reaction ; Polymorphism, Genetic ; Promoter Regions, Genetic ; Risk Assessment ; Risk Factors
Chemical Substances	Carrier Proteins ; microsomal triglyceride transfer protein ; Glutamate-Cysteine Ligase (EC 6.3.2.2)
Language	English
Publishing date	2010-02
Publishing country	Australia
Document type	Journal Article
ZDB-ID	632882-9
ISSN	1440-1746 ; 0815-9319
ISSN (online)	1440-1746
ISSN	0815-9319
DOI	10.1111/j.1440-1746.2009.06001.x
Database	MEDical Literature Analysis and Retrieval System OnLINE

Full text online

Accessible to users with ZB MED library card

In stock of ZB MED Cologne/Königswinter

Zs.A 2180: Show issues			Location: Je nach Verfügbarkeit (siehe Angabe bei Bestand) bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular Jg. 1995 - 2021: Lesesall (1.OG) ab Jg. 2022: Lesesaal (EG)
Zs.MO 299: Show issues

Order via subito

Details ▾

Article: Hybrid Capture II and Polymerase Chain Reaction for Identifying HPV Infections in Samples Collected in a New Collection Medium

Nonogaki, Suely / Wakamatsu, Alda / Longatto Filho, Adhemar / Pereira, Sônia Maria Miranda / Utagawa, Maria Lúcia / FerreiraAlves, Venâncio Avancini / Di Loreto, Celso / Sakamoto Maeda, Marina Yoshiê / Lima, Temístocles P. / Roteli-Martins, Cecília / Syrjänen, Kari

Acta Cytologica

2011 Volume 48, Issue 4, Page(s) 514–520

Institution	From the Pathology Division, Adolfo Lutz Institute and Leonor Mendes de Barros Hospital, São Paulo, Brazil, and Istituto Superiore di Sanità, Rome, Italy
Language	English
Publishing date	2011-02-23
Publisher	S. Karger AG
Publishing place	Basel, Switzerland
Document type	Article
Note	Molecular Technique
ZDB-ID	80003-x
ISSN	1938-2650 ; 0001-5547
ISSN (online)	1938-2650
ISSN	0001-5547
DOI	10.1159/000326413
Database	Karger publisher's database

In stock of ZB MED Cologne/Königswinter

Zs.A 358: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
Jg. 1995 - 2021: Lesesall (1.OG)
ab Jg. 2022: Lesesaal (EG)

Order via subito

Details ▾
- See ZB MED holdings
- Order with fees

Article: Adrenocortical carcinoma: prognostic indices based on clinical and immunohistochemical markers.

Dichtchekenian, Vaê / de Bragança Pereira, Carlos Alberto / Kuperman, Hilton / Della Manna, Thais / Damiani, Durval / Ferreira Alves, Venancio Avancini / Filho, Adhemar Longato / Setian, Nuvarte

Journal of pediatric endocrinology & metabolism : JPEM

2005 Volume 18, Issue 4, Page(s) 347–353

Abstract: Adrenocortical carcinoma is a rare condition with an unpredictable prognosis as a rule. The authors retrospectively analyzed the clinical outcome of 46 patients (31 F, 15 M) during 16 years building up a numerical index for the prognosis, based on ... ...

Abstract	Adrenocortical carcinoma is a rare condition with an unpredictable prognosis as a rule. The authors retrospectively analyzed the clinical outcome of 46 patients (31 F, 15 M) during 16 years building up a numerical index for the prognosis, based on clinical and immunohistochemical data. Four indices were analyzed: J1= (Y + 2L + 4H)/T; J2 = (J1) square root of W/200; J3 = (O + Y + 2L + 4H)/T; J4 = (J3) square root W/200. Y = 1 when chronological age (CA) >33 mo, Y = 0 when CA < or =33 mo; L = 1 for right sided tumor and L = 0 for left sided tumor; H = 1 in presence of hypertension and H = 0 for normal blood pressure; T = length of disease in months; W = weight of tumor (g); O = 1 in the absence of p53 protein and O = 0 in the presence of p53. The chance of bad prognosis was observed when age is >33 mo, tumor is on the right side, systemic hypertension is present, tumor weight >250 g, in the absence of p53, J1, J2, J3 >0.4 (p <0.001) and J4 >0.5 (p <0.01). Clinical data and the mathematical model enabled us to establish probabilities of good prognosis in 78-96% and bad prognosis in 63-83%.
MeSH term(s)	Adolescent ; Adrenal Cortex Neoplasms/metabolism ; Adrenal Cortex Neoplasms/physiopathology ; Adrenocortical Carcinoma/metabolism ; Adrenocortical Carcinoma/physiopathology ; Biomarkers, Tumor/metabolism ; Child ; Child, Preschool ; Female ; Humans ; Infant ; Male ; Models, Biological ; Prognosis ; Retrospective Studies
Chemical Substances	Biomarkers, Tumor
Language	English
Publishing date	2005-03-30
Publishing country	Germany
Document type	Journal Article
ZDB-ID	1231070-0
ISSN	2191-0251 ; 0334-018X
ISSN (online)	2191-0251
ISSN	0334-018X
DOI	10.1515/jpem.2005.18.4.347
Database	MEDical Literature Analysis and Retrieval System OnLINE

In stock of ZB MED Cologne/Königswinter

Zs.A 2258: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
Jg. 1995 - 2021: Lesesall (1.OG)
ab Jg. 2022: Lesesaal (EG)

Order via subito

Details ▾
- See ZB MED holdings
- Order with fees

Article: Hybrid capture II and polymerase chain reaction for identifying HPV infections in samples collected in a new collection medium: a comparison.

Nonogaki, Suely / Wakamatsu, Alda / Longatto Filho, Adhemar / Pereira, Sonia Maria Miranda / Utagawa, Maria Lúcia / Ferreira Alves, Venâncio Avancini / Di Loreto, Celso / Sakamoto Maeda, Marina Yoshiê / Lima, Temístocles P / Roteli-Martins, Cecília / Syrjänen, Kari

Acta cytologica

2004 Volume 48, Issue 4, Page(s) 514–520

Abstract: Objective: To compare the performance of human papillomavirus (HPV) DNA detection by polymerase chain reaction (PCR) and Hybrid Capture II (HCII) test (Digene, Gaithersburg, Maryland, U.S.A.) in residual cells left in the collection vials of the ... ...

Abstract	Objective: To compare the performance of human papillomavirus (HPV) DNA detection by polymerase chain reaction (PCR) and Hybrid Capture II (HCII) test (Digene, Gaithersburg, Maryland, U.S.A.) in residual cells left in the collection vials of the DNACitoliq system (Digene Brasil, São Paulo, Brazil). Study design: A series of 263 cervical samples collected for liquid-based cytology with the DNACitoliq system was tested for oncogenic HPV types first with HCII and subsequently with PCR. After DNA purification with GFX Genomic Blood DNA Purification Kit (Amersham, Piscataway, New Jersey, U.S.A.), PCR was performed using AmpliTaq Gold DNA polymerase (Applied Biosystems). PGMY09/11 L1 consensus primers and GH20/PCO4 primers for human beta-globin target were coamplified. Results: Altogether, 260 samples were positive for beta-globin, and 3 negative ones were excluded from the analysis. PCR and HCII yielded concordant results in 199 cases (76.5%) (102 positive and 97 negative), with Cohen's kappa of .577 (95% CI .477-.677) and weighted kappa of .733 (95% CI .659-.791). HPV prevalence in different categories of cytologic abnormalities was practically identical with HCII and PCR assays (P=.989). Among the 61 (23.5%) discrepant cases, 28 samples were HCII+/PCR- cases. Of these, 27 of 28 samples showed a low viral load, and 1 had an intermediate viral load. Conclusion: The data suggest that residual material from the DNACitoliq system adequately preserves HPV DNA for detection by HCII and PCR, with performance similar to that of specimen transport medium.
MeSH term(s)	Adolescent ; Adult ; Biopsy/methods ; DNA Probes, HPV ; DNA, Viral/analysis ; Female ; Genotype ; Humans ; Middle Aged ; Papillomaviridae/classification ; Papillomaviridae/genetics ; Papillomaviridae/isolation & purification ; Papillomavirus Infections/diagnosis ; Papillomavirus Infections/virology ; Polymerase Chain Reaction ; Reagent Kits, Diagnostic ; Reproducibility of Results ; Specimen Handling/instrumentation ; Specimen Handling/methods
Chemical Substances	DNA Probes, HPV ; DNA, Viral ; Reagent Kits, Diagnostic
Language	English
Publishing date	2004-07
Publishing country	Switzerland
Document type	Comparative Study ; Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	80003-x
ISSN	1938-2650 ; 0001-5547
ISSN (online)	1938-2650
ISSN	0001-5547
Database	MEDical Literature Analysis and Retrieval System OnLINE

In stock of ZB MED Cologne/Königswinter

Zs.A 358: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
Jg. 1995 - 2021: Lesesall (1.OG)
ab Jg. 2022: Lesesaal (EG)

Order via subito

Details ▾
- See ZB MED holdings
- Order with fees

To top

More links

Kategorien

Order via subito

Inter-library loan at ZB MED

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

More links

Kategorien

Order via subito

Inter-library loan at ZB MED

Full text online

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito