LIVIVO - Search results -

Search results

Result 1 - 10 of total 23

Search options

Article ; Online: Standardized guidelines for Africanized honeybee venom production needed for development of new apilic antivenom.

Oliveira Orsi, Ricardo / Zaluski, Rodrigo / de Barros, Luciana Curtolo / Barraviera, Benedito / Pimenta, Daniel Carvalho / Ferreira Junior, Rui Seabra

Journal of toxicology and environmental health. Part B, Critical reviews

2024 Volume 27, Issue 2, Page(s) 73–90

Abstract: Africanized bees have spread across the Americas since 1956 and consequently resulted in human and animal deaths attributed to massive attacks related to exposure from Argentina to the USA. In Brazil, more than 100,000 accidents were registered in the ... ...

Abstract	Africanized bees have spread across the Americas since 1956 and consequently resulted in human and animal deaths attributed to massive attacks related to exposure from Argentina to the USA. In Brazil, more than 100,000 accidents were registered in the last 5 years with a total of 303 deaths. To treat such massive attacks, Brazilian researchers developed the first specific antivenom against Africanized honey bee sting exposure. This unique product, the first of its kind in the world, has been safely tested in 20 patients during a Phase 2 clinical trial. To develop the antivenom, a standardized process was undertaken to extract primary venom antigens from the Africanized bees for immunization of serum-producing horses. This process involved extracting, purifying, fractionating, characterizing, and identifying the venom (apitoxin) employing mass spectrometry to generate standardized antigen for hyperimmunization of horses using the major toxins (melittin and its isoforms and phospholipase A2). The current guide describes standardization of the entire production chain of venom antigens in compliance with good manufacturing practices (GMP) required by regulatory agencies. Emphasis is placed upon the welfare of bees and horses during this process, as well as the development of a new biopharmaceutical to ultimately save lives.
MeSH term(s)	Bees ; Humans ; Animals ; Antivenins/therapeutic use ; Insect Bites and Stings/drug therapy ; Bee Venoms/analysis ; Bee Venoms/chemistry ; Melitten/analysis ; Melitten/chemistry ; Phospholipases A2 ; Antigens
Chemical Substances	Antivenins ; Bee Venoms ; Melitten (20449-79-0) ; Phospholipases A2 (EC 3.1.1.4) ; Antigens
Language	English
Publishing date	2024-01-26
Publishing country	England
Document type	Journal Article ; Review
ZDB-ID	1415246-0
ISSN	1521-6950 ; 1093-7404
ISSN (online)	1521-6950
ISSN	1093-7404
DOI	10.1080/10937404.2023.2300786
Database	MEDical Literature Analysis and Retrieval System OnLINE

Full text online

Accessible to users with ZB MED library card

In stock of ZB MED Cologne/Königswinter

Zs.A 4944: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
Jg. 1995 - 2021: Lesesall (2.OG)
ab Jg. 2022: Lesesaal (EG)

Order via subito

This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

Details ▾

Article: Unveiling the Pain Relief Potential: Harnessing Analgesic Peptides from Animal Venoms.

Pereira, Ana Flávia Marques / Cavalcante, Joeliton S / Angstmam, Davi Gomes / Almeida, Cayo / Soares, Gean S / Pucca, Manuela B / Ferreira Junior, Rui Seabra

Pharmaceutics

2023 Volume 15, Issue 12

Abstract: The concept of pain encompasses a complex interplay of sensory and emotional experiences associated with actual or potential tissue damage. Accurately describing and localizing pain, whether acute or chronic, mild or severe, poses a challenge due to its ... ...

Abstract	The concept of pain encompasses a complex interplay of sensory and emotional experiences associated with actual or potential tissue damage. Accurately describing and localizing pain, whether acute or chronic, mild or severe, poses a challenge due to its diverse manifestations. Understanding the underlying origins and mechanisms of these pain variations is crucial for effective management and pharmacological interventions. Derived from a wide spectrum of species, including snakes, arthropods, mollusks, and vertebrates, animal venoms have emerged as abundant repositories of potential biomolecules exhibiting analgesic properties across a broad spectrum of pain models. This review focuses on highlighting the most promising venom-derived toxins investigated as potential prototypes for analgesic drugs. The discussion further encompasses research prospects, challenges in advancing analgesics, and the practical application of venom-derived toxins. As the field continues its evolution, tapping into the latent potential of these natural bioactive compounds holds the key to pioneering approaches in pain management and treatment. Therefore, animal toxins present countless possibilities for treating pain caused by different diseases. The development of new analgesic drugs from toxins is one of the directions that therapy must follow, and it seems to be moving forward by recommending the composition of multimodal therapy to combat pain.
Language	English
Publishing date	2023-12-13
Publishing country	Switzerland
Document type	Journal Article ; Review
ZDB-ID	2527217-2
ISSN	1999-4923
ISSN	1999-4923
DOI	10.3390/pharmaceutics15122766
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Article: Photobiomodulation Therapy Improves Repair of Bone Defects Filled by Inorganic Bone Matrix and Fibrin Heterologous Biopolymer.

Vigliar, Maria Fernanda Rossi / Marega, Lais Furlaneto / Duarte, Marco Antonio Hungaro / Alcalde, Murilo Priori / Rosso, Marcelie Priscila de Oliveira / Ferreira Junior, Rui Seabra / Barraviera, Benedito / Reis, Carlos Henrique Bertoni / Buchaim, Daniela Vieira / Buchaim, Rogerio Leone

Bioengineering (Basel, Switzerland)

2024 Volume 11, Issue 1

Abstract: Biomaterials are used extensively in graft procedures to correct bone defects, interacting with the body without causing adverse reactions. The aim of this pre-clinical study was to analyze the effects of photobiomodulation therapy (PBM) with the use of ... ...

Abstract	Biomaterials are used extensively in graft procedures to correct bone defects, interacting with the body without causing adverse reactions. The aim of this pre-clinical study was to analyze the effects of photobiomodulation therapy (PBM) with the use of a low-level laser in the repair process of bone defects filled with inorganic matrix (IM) associated with heterologous fibrin biopolymer (FB). A circular osteotomy of 4 mm in the left tibia was performed in 30 Wistar male adult rats who were randomly divided into three groups: G1 = IM + PBM, G2 = IM + FB and G3 = IM + FB + PBM. PBM was applied at the time of the experimental surgery and three times a week, on alternate days, until euthanasia, with 830 nm wavelength, in two points of the operated site. Five animals from each group were euthanized 14 and 42 days after surgery. In the histomorphometric analysis, the percentage of neoformed bone tissue in G3 (28.4% ± 2.3%) was higher in relation to G1 (24.1% ± 2.91%) and G2 (22.2% ± 3.11%) at 14 days and at 42 days, the percentage in G3 (35.1% ± 2.55%) was also higher in relation to G1 (30.1% ± 2.9%) and G2 (31.8% ± 3.12%). In the analysis of the birefringence of collagen fibers, G3 showed a predominance of birefringence between greenish-yellow in the neoformed bone tissue after 42 days, differing from the other groups with a greater presence of red-orange fibers. Immunohistochemically, in all experimental groups, it was possible to observe immunostaining for osteocalcin (OCN) near the bone surface of the margins of the surgical defect and tartrate-resistant acid phosphatase (TRAP) bordering the newly formed bone tissue. Therefore, laser photobiomodulation therapy contributed to improving the bone repair process in tibial defects filled with bovine biomaterial associated with fibrin biopolymer derived from snake venom.
Language	English
Publishing date	2024-01-13
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2746191-9
ISSN	2306-5354
ISSN	2306-5354
DOI	10.3390/bioengineering11010078
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Article ; Online: Challenges and Opportunities in Clinical Diagnostic Routine of Envenomation Using Blood Plasma Proteomics.

Cavalcante, Joeliton Dos Santos / de Almeida, Denis Emanuel Garcia / Moraes, Micael Saggion / Santos, Sophia Ribeiro / Pincinato, Pedro Moriel / Riciopo, Pedro Marques / de Oliveira, Laís Lacerda B / Monteiro, Wuelton Marcelo / Ferreira-Junior, Rui Seabra

Toxins

2023 Volume 15, Issue 3

Abstract: Specific and sensitive tools for the diagnosis and monitoring of accidents by venomous animals are urgently needed. Several diagnostic and monitoring assays have been developed; however, they have not yet reached the clinic. This has resulted in late ... ...

Abstract	Specific and sensitive tools for the diagnosis and monitoring of accidents by venomous animals are urgently needed. Several diagnostic and monitoring assays have been developed; however, they have not yet reached the clinic. This has resulted in late diagnoses, which represents one of the main causes of progression from mild to severe disease. Human blood is a protein-rich biological fluid that is routinely collected in hospital settings for diagnostic purposes, which can translate research progress from the laboratory to the clinic. Although it is a limited view, blood plasma proteins provide information about the clinical picture of envenomation. Proteome disturbances in response to envenomation by venomous animals have been identified, allowing mass spectrometry (MS)-based plasma proteomics to emerge as a tool in a range of clinical diagnostics and disease management that can be applied to cases of venomous animal envenomation. Here, we provide a review of the state of the art on routine laboratory diagnoses of envenomation by snakes, scorpions, bees, and spiders, as well as a review of the diagnostic methods and the challenges encountered. We present the state of the art on clinical proteomics as the standardization of procedures to be performed within and between research laboratories, favoring a more excellent peptide coverage of candidate proteins for biomarkers. Therefore, the selection of a sample type and method of preparation should be very specific and based on the discovery of biomarkers in specific approaches. However, the sample collection protocol (e.g., collection tube type) and the processing procedure of the sample (e.g., clotting temperature, time allowed for clotting, and anticoagulant used) are equally important to eliminate any bias.
MeSH term(s)	Animals ; Humans ; Proteomics/methods ; Snakes ; Blood Proteins/analysis ; Biomarkers ; Proteome ; Plasma/chemistry
Chemical Substances	Blood Proteins ; Biomarkers ; Proteome
Language	English
Publishing date	2023-02-27
Publishing country	Switzerland
Document type	Journal Article ; Review ; Research Support, Non-U.S. Gov't
ZDB-ID	2518395-3
ISSN	2072-6651 ; 2072-6651
ISSN (online)	2072-6651
ISSN	2072-6651
DOI	10.3390/toxins15030180
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Article: Morphofunctional Improvement of the Facial Nerve and Muscles with Repair Using Heterologous Fibrin Biopolymer and Photobiomodulation.

Bueno, Cleuber Rodrigo de Souza / Tonin, Maria Clara Cassola / Buchaim, Daniela Vieira / Barraviera, Benedito / Ferreira Junior, Rui Seabra / Santos, Paulo Sérgio da Silva / Reis, Carlos Henrique Bertoni / Pastori, Cláudio Maldonado / Pereira, Eliana de Souza Bastos Mazuqueli / Nogueira, Dayane Maria Braz / Cini, Marcelo Augusto / Rosa Junior, Geraldo Marco / Buchaim, Rogerio Leone

Pharmaceuticals (Basel, Switzerland)

2023 Volume 16, Issue 5

Abstract: Peripheral nerve injuries impair the patient's functional capacity, including those occurring in the facial nerve, which require effective medical treatment. Thus, we investigated the use of heterologous fibrin biopolymer (HFB) in the repair of the ... ...

Abstract	Peripheral nerve injuries impair the patient's functional capacity, including those occurring in the facial nerve, which require effective medical treatment. Thus, we investigated the use of heterologous fibrin biopolymer (HFB) in the repair of the buccal branch of the facial nerve (BBFN) associated with photobiomodulation (PBM), using a low-level laser (LLLT), analyzing the effects on axons, muscles facials, and functional recovery. This experimental study used twenty-one rats randomly divided into three groups of seven animals, using the BBFN bilaterally (the left nerve was used for LLLT): Control group-normal and laser (CGn and CGl); Denervated group-normal and laser (DGn and DGl); Experimental Repair Group-normal and laser (ERGn and ERGl). The photobiomodulation protocol began in the immediate postoperative period and continued for 5 weeks with a weekly application. After 6 weeks of the experiment, the BBFN and the perioral muscles were collected. A significant difference (
Language	English
Publishing date	2023-04-27
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2193542-7
ISSN	1424-8247
ISSN	1424-8247
DOI	10.3390/ph16050653
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Article ; Online: Human dental pulp stem cell monolayer and spheroid therapy after spinal motor root avulsion in adult rats.

Paes, Sabrina Moreira / Castro, Mateus Vidigal de / Barbosa, Rafael Maza / Politti Cartarozzi, Luciana / Coser, Lilian de Oliveira / Kempe, Paula Regina Gelinski / Decarli, Monize Caiado / Moraes, Ângela Maria / Barraviera, Benedito / Ferreira Júnior, Rui Seabra / Oliveira, Alexandre Leite Rodrigues de

Brain research

2022 Volume 1802, Page(s) 148229

Abstract: Spinal cord injuries result in severe neurological deficits and neuronal loss, with poor functional recovery. Mesenchymal stem cells have shown promising results; therefore the present objective of this work was to compare motor recovery after treatment ... ...

Abstract	Spinal cord injuries result in severe neurological deficits and neuronal loss, with poor functional recovery. Mesenchymal stem cells have shown promising results; therefore the present objective of this work was to compare motor recovery after treatment with human dental pulp stem cells (hDPSC) cultivated in monolayer (2D) or as spheroids (3D), following avulsion and reimplantation of spinal motor roots in adult rats. Thus, 72 adult female Lewis rats were divided into 4 groups: avulsion (AV); avulsion followed by reimplantation (AR); avulsion associated with reimplant and 2D cell therapy (AR + 2D), and avulsion associated with reimplant and 3D cell therapy (AR + 3D). The application of the cells in 2D and 3D was performed by microsurgery, with subsequent functional assessment using a walking track test (Catwalk system), immunohistochemistry, neuronal survival, and qRT-PCR in 1-, 4-, and 12-weeks post-injury. The animals in the AR + 2D and AR + 3D groups showed the highest neuronal survival rates, and immunofluorescence revealed downregulation of GFAP, and Iba-1, with preservation of synaptophysin, indicating a reduction in glial reactivity, combined with the maintenance of pre-synaptic inputs. There was an increase in anti-inflammatory (IL-4, TGFβ) and a reduction of pro-inflammatory factors (IL-6, TNFα) in animals treated with reimplantation and hDPSC. As for the functional recovery, in all analyzed parameters, the AR + 2D group performed better and was superior to the avulsion alone. Overall, our results indicate that the 2D and 3D cell therapy approaches provide successful immunomodulation and motor recovery, consistent with advanced therapies after spinal cord injury.
MeSH term(s)	Adult ; Animals ; Female ; Humans ; Rats ; Dental Pulp ; Motor Neurons/physiology ; Rats, Inbred Lew ; Spinal Cord ; Spinal Cord Injuries/therapy ; Spinal Nerve Roots/injuries ; Spinal Nerve Roots/physiology ; Stem Cells ; Cell Culture Techniques
Language	English
Publishing date	2022-12-30
Publishing country	Netherlands
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	1200-2
ISSN	1872-6240 ; 0006-8993
ISSN (online)	1872-6240
ISSN	0006-8993
DOI	10.1016/j.brainres.2022.148229
Database	MEDical Literature Analysis and Retrieval System OnLINE

Full text online

Accessible to users with ZB MED library card

In stock of ZB MED Cologne/Königswinter

Zs.A 161: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
Jg. 1995 - 2021: Lesesall (1.OG)
ab Jg. 2022: Lesesaal (EG)

Order via subito

Details ▾

Article: Phosphodiesterase-5 inhibitor sildenafil attenuates kidney injury induced by Bothrops alternatus snake venom

Jorge, Antônio Rafael Coelho / Marinho, Aline Diogo / Silveira, João Alison de Moraes / Nogueira Junior, Francisco Assis / de Aquino, Pedro Everson Alexandre / Alves, Ana Paula Negreiros Nunes / Jorge, Roberta Jeane Bezerra / Ferreira Junior, Rui Seabra / Monteiro, Helena Serra Azul

Toxicon. 2021 Oct. 30, v. 202

2021

Abstract: Acute kidney injury pathogenesis in envenoming by snakes is multifactorial and involves immunologic reactions, hemodynamic disturbances, and direct nephrotoxicity. Sildenafil (SFC), a phosphodiesterase 5 inhibitor, has been reported to protect against ... ...

Abstract	Acute kidney injury pathogenesis in envenoming by snakes is multifactorial and involves immunologic reactions, hemodynamic disturbances, and direct nephrotoxicity. Sildenafil (SFC), a phosphodiesterase 5 inhibitor, has been reported to protect against pathological kidney changes.This study aimed to investigate the protective effect of sildenafil against Bothrops alternatus snake venom (BaV)-induced nephrotoxicity.Kidneys from Wistar rats (n = 6, weighing 260–300 g) were isolated and divided into four groups: (1) perfused with a modified Krebs-Henseleit solution (MKHS) containing 6 g% of bovine serum albumin; (2) administered 3 μg/mL SFC; (3) perfused with 3 μg/mL BaV; and (4) administered SFC + BaV, both at 3 μg/mL. Subsequently, the perfusion pressure (PP), renal vascular resistance (RVR), urinary flow (UF), glomerular filtration rate (GFR), and percentage of electrolyte tubular sodium and chloride transport (%TNa⁺, %TCl⁻, respectively) were evaluated. The cyclic guanosine monophosphate (cGMP) levels were analyzed in the perfusate, and the kidneys were removed to perform oxidative stress and histopathological analyses.All renal parameters evaluated were reduced with BaV. In the SFC + BaV group, SFC restored PP to normal values and promoted a significant increase in %TNa⁺ and %TCl⁻. cGMP levels were increased in the SFC + BaV group. The oxidative stress biomarkers, malondialdehyde (MDA) and glutathione (GSH), were reduced by BaV. In the SFC + BaV group, a decrease in MDA without an increase in GSH was observed. These findings were confirmed by histological analysis, which showed improvement mainly in tubulis.Our data suggest the involvement of phosphodiesterase-5 and cGMP in BaV-induced nephrotoxicity since its effects were attenuated by the administration of SFC.
Keywords	Bothrops ; acute kidney injury ; biomarkers ; bovine serum albumin ; chlorides ; cyclic GMP ; electrolytes ; glomerular filtration rate ; glutathione ; hemodynamics ; histology ; histopathology ; kidneys ; malondialdehyde ; nephrotoxicity ; oxidative stress ; pathogenesis ; protective effect ; snake venoms ; sodium ; urea cycle
Language	English
Dates of publication	2021-1030
Size	p. 46-52.
Publishing place	Elsevier Ltd
Document type	Article
ZDB-ID	204479-1
ISSN	1879-3150 ; 0041-0101
ISSN (online)	1879-3150
ISSN	0041-0101
DOI	10.1016/j.toxicon.2021.08.024
Database	NAL-Catalogue (AGRICOLA)

Full text online

Accessible to users with ZB MED library card

In stock of ZB MED Cologne/Königswinter

Zs.A 501: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
Jg. 1995 - 2021: Lesesall (1.OG)
ab Jg. 2022: Lesesaal (EG)

Order via subito

Details ▾

Article ; Online: Phosphodiesterase-5 inhibitor sildenafil attenuates kidney injury induced by Bothrops alternatus snake venom.

Toxicon : official journal of the International Society on Toxinology

2021 Volume 202, Page(s) 46–52

Abstract	Acute kidney injury pathogenesis in envenoming by snakes is multifactorial and involves immunologic reactions, hemodynamic disturbances, and direct nephrotoxicity. Sildenafil (SFC), a phosphodiesterase 5 inhibitor, has been reported to protect against pathological kidney changes. Objective: This study aimed to investigate the protective effect of sildenafil against Bothrops alternatus snake venom (BaV)-induced nephrotoxicity. Methods: Kidneys from Wistar rats (n = 6, weighing 260-300 g) were isolated and divided into four groups: (1) perfused with a modified Krebs-Henseleit solution (MKHS) containing 6 g% of bovine serum albumin; (2) administered 3 μg/mL SFC; (3) perfused with 3 μg/mL BaV; and (4) administered SFC + BaV, both at 3 μg/mL. Subsequently, the perfusion pressure (PP), renal vascular resistance (RVR), urinary flow (UF), glomerular filtration rate (GFR), and percentage of electrolyte tubular sodium and chloride transport (%TNa Results: All renal parameters evaluated were reduced with BaV. In the SFC + BaV group, SFC restored PP to normal values and promoted a significant increase in %TNa Conclusion: Our data suggest the involvement of phosphodiesterase-5 and cGMP in BaV-induced nephrotoxicity since its effects were attenuated by the administration of SFC.
MeSH term(s)	Animals ; Bothrops ; Cyclic Nucleotide Phosphodiesterases, Type 5 ; Kidney ; Phosphodiesterase 5 Inhibitors/therapeutic use ; Rats ; Rats, Wistar ; Sildenafil Citrate/therapeutic use ; Snake Venoms/toxicity
Chemical Substances	Phosphodiesterase 5 Inhibitors ; Snake Venoms ; Sildenafil Citrate (BW9B0ZE037) ; Cyclic Nucleotide Phosphodiesterases, Type 5 (EC 3.1.4.35)
Language	English
Publishing date	2021-09-10
Publishing country	England
Document type	Journal Article
ZDB-ID	204479-1
ISSN	1879-3150 ; 0041-0101
ISSN (online)	1879-3150
ISSN	0041-0101
DOI	10.1016/j.toxicon.2021.08.024
Database	MEDical Literature Analysis and Retrieval System OnLINE

Full text online

Accessible to users with ZB MED library card

In stock of ZB MED Cologne/Königswinter

Zs.A 501: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
Jg. 1995 - 2021: Lesesall (1.OG)
ab Jg. 2022: Lesesaal (EG)

Order via subito

Details ▾

Article ; Online: Honey bee envenoming in Santa Catarina, Brazil, 2007 through 2017: an observational, retrospective cohort study.

Oliveira, Suzana Kniphoff de / Trevisol, Daisson José / Parma, Gabriel Cremona / Ferreira Júnior, Rui Seabra / Barbosa, Alexandre Naime / Barraviera, Benedito / Schuelter-Trevisol, Fabiana

Revista da Sociedade Brasileira de Medicina Tropical

2019 Volume 52, Page(s) e20180418

Abstract: Introduction: We evaluated the epidemiological and clinical profile of reported cases of bee sting incidents in Santa Catarina, Brazil.: Methods: This retrospective cohort study included all reported cases of bee sting incidents among the population ... ...

Abstract	Introduction: We evaluated the epidemiological and clinical profile of reported cases of bee sting incidents in Santa Catarina, Brazil. Methods: This retrospective cohort study included all reported cases of bee sting incidents among the population of Santa Catarina from 2007 to 2017. Results: In total, 8,912 cases were reported, corresponding to an overall rate of 12.3/100,000 population. The mean age was 29,8 years with 60.2% men. The lethality rate was 0.2%. Conclusions: Santa Catarina has a high incidence rate of bee stings, which is higher than the national average. The data presented in this study may be underestimated.
MeSH term(s)	Adolescent ; Adult ; Aged ; Aged, 80 and over ; Animals ; Bee Venoms/poisoning ; Bees ; Brazil/epidemiology ; Child ; Child, Preschool ; Cohort Studies ; Disease Notification ; Female ; Humans ; Incidence ; Infant ; Infant, Newborn ; Insect Bites and Stings/mortality ; Male ; Middle Aged ; Retrospective Studies ; Spatial Analysis ; Young Adult
Chemical Substances	Bee Venoms
Language	English
Publishing date	2019-04-11
Publishing country	Brazil
Document type	Journal Article ; Observational Study
ZDB-ID	1038126-0
ISSN	1678-9849 ; 0037-8682
ISSN (online)	1678-9849
ISSN	0037-8682
DOI	10.1590/0037-8682-0418-2018
Database	MEDical Literature Analysis and Retrieval System OnLINE

In stock of ZB MED Cologne/Königswinter

Zs.A 2726: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
Jg. 1995 - 2021: Lesesall (2.OG)
ab Jg. 2022: Lesesaal (EG)

Order via subito

Details ▾
- See ZB MED holdings
- Order with fees

Article ; Online: Effects of fibrin sealant and bone fragments on defect regeneration performed on rat tibiae: An experimental study.

de Oliveira, Carla Teresa Barbosa / Leonel, Bruna Carlos / de Oliveira, Ana Clara / de Brito Paiva, Marcela / Ramos, Junia / Barraviera, Benedito / Ferreira Junior, Rui Seabra / Shimano, Antônio Carlos

Journal of the mechanical behavior of biomedical materials

2020 Volume 104, Page(s) 103662

Abstract: Fibrin sealant (FS) is a biomaterial that exhibits hemostatic and repairing properties. It has been successfully used as scaffolds and adhesives to improve repair and regeneration of tissues. The objective of this study was to evaluate the effect of FS ... ...

Abstract	Fibrin sealant (FS) is a biomaterial that exhibits hemostatic and repairing properties. It has been successfully used as scaffolds and adhesives to improve repair and regeneration of tissues. The objective of this study was to evaluate the effect of FS in the regeneration process of bone defects in male rat tibias through macroscopic, microscopic and mechanical analysis. A bone defect of 2.9 mm was performed on the medial face of the proximal third of the tibia of 40 rats and implanted FS and autologous bone graft (AG). The animals were divided into four groups: animals with bone defect without any treatment (CON), animals treated with fibrin sealant (TFS), animals treated with autologous graft (TAG) and animals treated with fibrin sealant and autologous graft (FSAG). The animals were euthanized 42 days after surgery. Macroscopic analysis showed no difference between the groups (p > 0.05) in relation to tibial weight, but a statistically significant difference (p = 0.005) was observed for their length. Micro-computed tomography (micro-CT) revealed tendentious values regarding bone microarchitecture and FS. Bone mineral densitometry (BMD) showed significance between the FSAG (p = 0.009) and TFS (p = 0.007) groups. The bone mineral content (BMC) presented a significant difference between all groups (p = 0.020). Maximum strength showed a significant difference between the FSAG group (p = 0.007) and the others. The results obtained in relation to the relative stiffness also present a significant difference (p = 0.023). Newly formed bone showed significant differences between groups (p = 0.035). We conclude that bone defect regeneration was directly influenced by the use of FS and AG.
MeSH term(s)	Animals ; Bone Regeneration ; Bone Transplantation ; Fibrin Tissue Adhesive/pharmacology ; Male ; Rats ; Tibia/diagnostic imaging ; X-Ray Microtomography
Chemical Substances	Fibrin Tissue Adhesive
Language	English
Publishing date	2020-01-29
Publishing country	Netherlands
Document type	Journal Article
ZDB-ID	2378381-3
ISSN	1878-0180 ; 1751-6161
ISSN (online)	1878-0180
ISSN	1751-6161
DOI	10.1016/j.jmbbm.2020.103662
Database	MEDical Literature Analysis and Retrieval System OnLINE

Full text online

Accessible to users with ZB MED library card

Order via subito

Details ▾
- Full text online
- Order with fees

To top

Full text online

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

More links

Kategorien

Order via subito

More links

Kategorien

Order via subito

More links

Kategorien

Order via subito

More links

Kategorien

Order via subito

Full text online

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

Full text online

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

Full text online

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

Full text online

More links

Kategorien

Order via subito