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  1. Article ; Online: The role of hepatitis C virus genotypes and core mutations in hepatocellular carcinoma in Cameroon.

    Amougou-Atsama, Marie / Jean Adrien Atangana, Paul / Noah Noah, Dominique / Fewou Moundipa, Paul / Pineau, Pascal / Njouom, Richard

    Journal of viral hepatitis

    2020  Volume 27, Issue 9, Page(s) 880–885

    Abstract: Background: Hepatitis C virus (HCV) infection is known to be an important risk factor for hepatocellular carcinoma (HCC) in Cameroon. However, the effect of HCV-related factors on HCC development still remains unknown in the Central Africa. In this ... ...

    Abstract Background: Hepatitis C virus (HCV) infection is known to be an important risk factor for hepatocellular carcinoma (HCC) in Cameroon. However, the effect of HCV-related factors on HCC development still remains unknown in the Central Africa. In this study, we investigated the role of HCV genotypes and core mutations in HCC development in Cameroonian patients.
    Methods: A case-control study was conducted using patients with HCV-related HCC and matched controls individuals with chronic HCV infection but without HCC. HCV genotypes and mutations were determined using a hemi-nested amplification and sequencing analysis focus on the core and NS5B HCV regions.
    Results: We identify HCV genotype 1, 2 and 4 in both groups. Interestingly, genotype 4 was significantly more prevalent in HCC patients (53.3%). Overall, distribution of genotypes was very different between cases and controls (P = 4.2 E-7). The risk factors analysis showed that infection with HCV-4 is strongly associated with HCC development with odd ratio, 95% confidence interval and p-values of 7.4 (95% CI: 2.08-26.6; P = .001). Furthermore, the risk of developing HCC increased even more significantly in case of infection with HCV subtype 4f with the odd ratio of 20.8 (95% CI, 4.1-66.8; P < .001). Mutations K10R, T72E, K74R and G77A were significantly more frequent in patients with HCC. Remarkably, HCV-4f isolates from HCC patients carried significantly more mutations when compared to controls with HCV-4f or others genotypes (P = .0001).
    Conclusions: Our results indicate that patients infected with HCV-4f or with selected variants affecting HCV core gene are at increased risk to develop HCC.
    MeSH term(s) Cameroon ; Carcinoma, Hepatocellular/virology ; Case-Control Studies ; Genotype ; Hepacivirus/genetics ; Hepatitis C/virology ; Humans ; Liver Neoplasms/virology ; Mutation
    Language English
    Publishing date 2020-05-28
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 1212497-7
    ISSN 1365-2893 ; 1352-0504
    ISSN (online) 1365-2893
    ISSN 1352-0504
    DOI 10.1111/jvh.13303
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 4119: Show issues Location:
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  2. Article ; Online: Bisphenol A differentially affects male reproductive function biomarkers in a reference population and agro pesticides users from Djutitsa, Cameroon.

    Manfo, Faustin Pascal Tsagué / Harthé, Cathérine / Nantia, Edouard Akono / Dechaud, Henri / Tchana, Angèle Nkouatchoua / Zabot, Marie-Tthérèse / Pugeat, Michel / Fewou Moundipa, Paul

    Toxicology and industrial health

    2019  Volume 35, Issue 4, Page(s) 324–335

    Abstract: The consequences of bisphenol A (BPA) exposure on male reproductive function were studied in two populations from Cameroon, farmers using agro pesticides in Djutitsa (rural area) and townsmen in Yaoundé (urban area, Centre region). Urinary BPA ... ...

    Abstract The consequences of bisphenol A (BPA) exposure on male reproductive function were studied in two populations from Cameroon, farmers using agro pesticides in Djutitsa (rural area) and townsmen in Yaoundé (urban area, Centre region). Urinary BPA concentration from all participants was measured, and the values were correlated with biochemical markers of male reproductive function. The data showed that BPA could be detected in 92.6% of urine participants, with an average concentration of 2.18 ± 1.97 µg/g creatinine but with no significant difference between the urinary BPA concentration from rural and urban populations. From BPA urinary concentration, the BPA average daily intake was estimated to be 0.06 ± 0.05 μg/kg/day (3.51 µg/day per individual) in the Cameroon population. Interestingly, free and bioavailable testosterone concentrations and estradiol/testosterone ratio correlated with BPA levels in the overall population. When data were analysed according to residence, BPA correlated with total testosterone levels ( r = -0.433) and estradiol/testosterone ratio ( r = 0.338) in the urban residents only, while the rural population exhibited significant increases in sex-hormone-binding globulin with increased BPA exposure. Our data showed that the male Cameroon population is exposed to BPA but that inconstant BPA association to endocrine reproductive markers suggests that other environmental factors in combination with BPA exposure might influence testicular function.
    MeSH term(s) Adolescent ; Adult ; Benzhydryl Compounds/toxicity ; Benzhydryl Compounds/urine ; Cameroon ; Estradiol/metabolism ; Humans ; Male ; Middle Aged ; Pesticides ; Phenols/toxicity ; Phenols/urine ; Rural Population/statistics & numerical data ; Sex Hormone-Binding Globulin/metabolism ; Testosterone/metabolism ; Urban Population/statistics & numerical data ; Young Adult
    Chemical Substances Benzhydryl Compounds ; Pesticides ; Phenols ; Sex Hormone-Binding Globulin ; Testosterone (3XMK78S47O) ; Estradiol (4TI98Z838E) ; bisphenol A (MLT3645I99)
    Language English
    Publishing date 2019-04-25
    Publishing country England
    Document type Journal Article
    ZDB-ID 56831-4
    ISSN 1477-0393 ; 0748-2337
    ISSN (online) 1477-0393
    ISSN 0748-2337
    DOI 10.1177/0748233719838437
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 2268: Show issues Location:
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  3. Article: Effect of Solubilizers on the Androgenic Activity of

    Akono Nantia, Edouard / Pascal Tsagué Manfo, Faustin / Sara E Beboy, Nathalie / Fewou Moundipa, Paul

    Advanced pharmaceutical bulletin

    2017  Volume 7, Issue 1, Page(s) 103–108

    Abstract: Purpose: ...

    Abstract Purpose:
    Language English
    Publishing date 2017-04-13
    Publishing country Iran
    Document type Journal Article
    ZDB-ID 3018440-X
    ISSN 2251-7308 ; 2228-5881
    ISSN (online) 2251-7308
    ISSN 2228-5881
    DOI 10.15171/apb.2017.013
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article: In silico and in vitro screening of licensed antimalarial drugs for repurposing as inhibitors of hepatitis E virus.

    Tietcheu Galani, Borris Rosnay / Ayissi Owona, Vincent Brice / Guemmogne Temdie, Romeo Joel / Metzger, Karoline / Atsama Amougou, Marie / Djamen Chuisseu, Pascal Dieudonné / Fondjo Kouam, Arnaud / Ngounoue Djuidje, Marceline / Aliouat-Denis, Cécile-Marie / Cocquerel, Laurence / Fewou Moundipa, Paul

    In silico pharmacology

    2021  Volume 9, Issue 1, Page(s) 35

    Language English
    Publishing date 2021-05-04
    Publishing country Germany
    Document type Journal Article
    ZDB-ID 2702993-1
    ISSN 2193-9616
    ISSN 2193-9616
    DOI 10.1007/s40203-021-00093-y
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article ; Online: Enrichment in selected genotypes, basal core and precore mutations of hepatitis B virus in patients with hepatocellular carcinoma in Cameroon.

    Atsama Amougou, Marie / Marchio, Agnes / Bivigou-Mboumba, Berthold / Noah Noah, Dominique / Banai, Robert / Atangana, Paul Jean Adrien / Fewou Moundipa, Paul / Pineau, Pascal / Njouom, Richard

    Journal of viral hepatitis

    2019  Volume 26, Issue 9, Page(s) 1086–1093

    Abstract: Worldwide, the development of hepatocellular carcinoma (HCC) is known to be influenced by several hepatitis B viral factors. However, the effect of hepatitis B virus (HBV) genotypes and a landscape of nucleotide changes affecting the precore (PC) and ... ...

    Abstract Worldwide, the development of hepatocellular carcinoma (HCC) is known to be influenced by several hepatitis B viral factors. However, the effect of hepatitis B virus (HBV) genotypes and a landscape of nucleotide changes affecting the precore (PC) and basal core promoter (BCP) during infection leading to HCC remain largely unknown in the Central Africa region. Thus, we performed a case-control study on patients with HBV-related HCC and matched controls without HCC but with chronic HBV infection. Genotypes and mutation spectrums were evaluated using a hemi-nested amplification and sequencing analysis focused on the BCP and PC regions. We identified the co-circulation of HBV quasi-subgenotype A3 (QS-A3) and genotype E in both groups. Interestingly, HBV-QS-A3 was significantly more prevalent in patients with HCC (80.0%) than in controls (31.9%, P = 4.5 E-7, OR = 11.5, 95% CI: 3.8-38.5). HBV mutation spectra and nucleotide changes were significantly more polymorphic in patients with HCC. Remarkably, HCC patients infected with HBV-QS-A3 were significantly more mutated compared to patients infected with genotype E (P < 0.0001). In addition, G:C>T:A transversions, generally associated with aflatoxin B1 exposure in tropical regions, were significantly more prevalent in HCC patients infected either with HBV-QS-A3 or HBV genotype E (P = 2.2 E-05) when compared to controls. In conclusion, our results indicate that patients infected with HBV-QS-A3 are at increased risk to develop HCC. In addition, viral genomes isolated for patients with tumour are more heavily altered than those found in controls. Preferential targeting of these patients for antiviral treatment is of paramount importance to reduce future HCC incidence in Cameroon.
    MeSH term(s) Adolescent ; Adult ; Aged ; Cameroon/epidemiology ; Carcinoma, Hepatocellular/epidemiology ; Carcinoma, Hepatocellular/virology ; Case-Control Studies ; Child ; DNA, Viral/genetics ; Female ; Genotype ; Hepatitis B virus/genetics ; Hepatitis B, Chronic/complications ; Hepatitis B, Chronic/epidemiology ; Humans ; Incidence ; Liver Neoplasms/epidemiology ; Liver Neoplasms/virology ; Male ; Middle Aged ; Mutation ; Promoter Regions, Genetic ; Young Adult
    Chemical Substances DNA, Viral
    Language English
    Publishing date 2019-07-19
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 1212497-7
    ISSN 1365-2893 ; 1352-0504
    ISSN (online) 1365-2893
    ISSN 1352-0504
    DOI 10.1111/jvh.13131
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 4119: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
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  6. Article ; Online: Antioxidant fractions of Khaya grandifoliola C.DC. and Entada africana Guill. et Perr. induce nuclear translocation of Nrf2 in HC-04 cells.

    Njayou, Frédéric Nico / Amougou, Atsama Marie / Fouemene Tsayem, Romeo / Njikam Manjia, Jacqueline / Rudraiah, Swetha / Bradley, Bolling / Manautou, José Enrique / Fewou Moundipa, Paul

    Cell stress & chaperones

    2015  Volume 20, Issue 6, Page(s) 991–1000

    Abstract: The in vitro antioxidant properties, cytoprotective activity, and ability to induce nuclear translocation of nuclear factor E2-related factor-2 (Nrf-2) of five solvent fractions of the methylene chloride/methanol (1:1 v/v) extract of Khaya grandifoliola ( ...

    Abstract The in vitro antioxidant properties, cytoprotective activity, and ability to induce nuclear translocation of nuclear factor E2-related factor-2 (Nrf-2) of five solvent fractions of the methylene chloride/methanol (1:1 v/v) extract of Khaya grandifoliola (Meliaceae) and Entada africana (Fabaceae) were evaluated. Five antioxidant endpoints were used in the antioxidant activity investigation. The total phenolic content of the fractions was assessed as to the Folin-Ciocalteu method and the profile of interesting fractions analyzed by high-performance liquid chromatography (HPLC). The cytoprotective activity of fractions was determined by H2O2-induced oxidative stress in HC-04 cells by measuring lactate dehydrogenase (LDH) leakage into culture medium. HC-04 cells were used to investigate the ability to induce nuclear translocation of Nrf2. For both plants, the methylene chloride/methanol (90/10; v/v) fraction (F10), methylene chloride/methanol (75/25; v/v) (F25), and the methanolic fraction (F100) were found to have the highest total polyphenol content and exhibited high antioxidant activity strongly correlated with total polyphenol content. The cytoprotective activity of fraction F25 from both plants was comparable to that of quercetin (3.40 ± 0.05 μg/mL), inhibiting LDH leakage with a low half inhibition concentration (IC50) of 4.05 ± 0.03 and 3.8 ± 0.02 μg/mL for K. grandifoliola and E. africana, respectively. Lastly, fraction F25 of K. grandifoliola significantly (P < 0.05) induced nuclear Nrf2 translocation by sixfold, whereas that from E. africana and quercetin was only twofold. The results indicate for the first time that fraction F25 of the studied plants is more antioxidant and cytoprotective and induces nuclear translocation of Nrf2 in a human hepatocyte cell line.
    MeSH term(s) Active Transport, Cell Nucleus/drug effects ; Animals ; Antioxidants/chemistry ; Antioxidants/pharmacology ; Cell Line ; Cell Survival/drug effects ; Fabaceae/chemistry ; Humans ; Hydrogen Peroxide/metabolism ; Meliaceae/chemistry ; NF-E2-Related Factor 2/metabolism ; Oxidative Stress/drug effects ; Plant Extracts/chemistry ; Plant Extracts/pharmacology ; Polyphenols/metabolism ; Rats
    Chemical Substances Antioxidants ; NF-E2-Related Factor 2 ; Plant Extracts ; Polyphenols ; Hydrogen Peroxide (BBX060AN9V)
    Language English
    Publishing date 2015-08-14
    Publishing country Netherlands
    Document type Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
    ZDB-ID 1362749-1
    ISSN 1466-1268 ; 1355-8145
    ISSN (online) 1466-1268
    ISSN 1355-8145
    DOI 10.1007/s12192-015-0628-6
    Database MEDical Literature Analysis and Retrieval System OnLINE

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