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  1. Article: A paradigm shift in disaster management: Incorporating a human rights-based approach to disaster risk reduction.

    Ficara, Francesca / Wheeler, Monique

    Journal of emergency management (Weston, Mass.)

    2023  Volume 21, Issue 6, Page(s) 557–576

    Abstract: Understanding disasters as socially constructed events represents a departure from current and historic ways in which disasters are characterized, requiring a focal shift in thinking from forces of nature toward social order. Changing societal reactions ... ...

    Abstract Understanding disasters as socially constructed events represents a departure from current and historic ways in which disasters are characterized, requiring a focal shift in thinking from forces of nature toward social order. Changing societal reactions to evolving natural occurrences restores disasters within the social order, introducing law as an essential framework in approaching disasters as injustices as opposed to misfortunes. International attention is starting to shift strategies intended to reduce risks to natural or man-made hazards and increasing attention on methods toward minimizing their impact known as disaster risk reduction (DRR). DRR is "a policy aimed at preventing new and reducing existing disaster risk and managing residual risk, all of which contribute to strengthening resilience and therefore to the achievement of sustainable development." The development of normative frameworks to reinforce disaster governance is a significant component in enhancing disaster management systems. Disaster law is an emerging tool to regulate "governance, ethics, and decisions on the demands of a sustainable, inclusive, and healthy planet." International legal frameworks heavily influence disaster prevention and preparedness with an increased central focus on implementing International Human Rights Law in DRR practices. Legal structures protecting human rights in DRR initiatives positively obligate states to take proper and necessary actions to prevent harm from future disasters. The application of human rights standards fosters the paradigm shift from evaluation of the hazards impact toward assessments of states' negligence of risks. Interactions among the natural environment, socio-demographics, and the built environment are strong predictors for disaster losses, thus "the regulatory potential for avoiding disasters and reducing their consequence is obvious." Preventative action becomes a crucial element if the catalyst of the disaster event is failure to adequately prepare and social vulnerability. Disaster law encompasses participation, damage control, and local habitat management as mandatory conditions of governance, assigning criminal liability to public administrators' negligence toward disaster planning and/or enforcement. Disaster law produces a "sustainable, reliable, and cost-effective model for addressing disasters," empowering communities to participate in disaster management efforts, one of the strongest methods of building resilience and reducing risk to disasters.
    MeSH term(s) Humans ; Disasters ; Disaster Planning ; Administrative Personnel ; Human Rights
    Language English
    Publishing date 2023-04-25
    Publishing country United States
    Document type Journal Article
    ZDB-ID 2799989-0
    ISSN 1543-5865
    ISSN 1543-5865
    DOI 10.5055/jem.0748
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: Micro-RNAs: A safety net to protect hematopoietic stem cell self-renewal.

    Crisafulli, Laura / Ficara, Francesca

    Wiley interdisciplinary reviews. RNA

    2021  Volume 13, Issue 3, Page(s) e1693

    Abstract: The hematopoietic system is sustained over time by a small pool of hematopoietic stem cells (HSCs). They reside at the apex of a complex hierarchy composed of cells with progressively more restricted lineage potential, regenerative capacity, and with ... ...

    Abstract The hematopoietic system is sustained over time by a small pool of hematopoietic stem cells (HSCs). They reside at the apex of a complex hierarchy composed of cells with progressively more restricted lineage potential, regenerative capacity, and with different proliferation characteristics. Like other somatic stem cells, HSCs are endowed with long-term self-renewal and multipotent differentiation ability, to sustain the high turnover of mature cells such as erythrocytes or granulocytes, and to rapidly respond to acute peripheral stresses including bleeding, infections, or inflammation. Maintenance of both attributes over time, and of the proper balance between these opposite features, is crucial to ensure the homeostasis of the hematopoietic system. Micro-RNAs (miRNAs) are short non-coding RNAs that regulate gene expression posttranscriptionally upon binding to specific mRNA targets. In the past 10 years they have emerged as important players for preserving the HSC pool by acting on several biological mechanisms, such as maintenance of the quiescent state while preserving proliferation ability, prevention of apoptosis, premature differentiation, lineage skewing, excessive expansion, or retention within the BM niche. miRNA-mediated posttranscriptional fine-tuning of all these processes constitutes a safety mechanism to protect HSCs, by complementing the action of transcription factors and of other regulators and avoiding unwanted expansion or aplasia. The current knowledge of miRNAs function in different aspects of HSC biology, including consequences of aberrant miRNA expression, will be reviewed; yet unsolved issues will be discussed. This article is categorized under: RNA in Disease and Development > RNA in Disease RNA in Disease and Development > RNA in Development.
    MeSH term(s) Apoptosis ; Cell Differentiation/genetics ; Hematopoietic Stem Cells/metabolism ; Homeostasis ; MicroRNAs/genetics ; MicroRNAs/metabolism
    Chemical Substances MicroRNAs
    Language English
    Publishing date 2021-09-16
    Publishing country United States
    Document type Journal Article ; Review ; Research Support, Non-U.S. Gov't
    ZDB-ID 2634714-3
    ISSN 1757-7012 ; 1757-7004
    ISSN (online) 1757-7012
    ISSN 1757-7004
    DOI 10.1002/wrna.1693
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  3. Article: PBX1: a TALE of two seasons-key roles during development and in cancer.

    Crisafulli, Laura / Brindisi, Matteo / Liturri, Mirko Giuseppe / Sobacchi, Cristina / Ficara, Francesca

    Frontiers in cell and developmental biology

    2024  Volume 12, Page(s) 1372873

    Abstract: Pre-B cell leukemia factor 1 (PBX1) is a Three Aminoacid Loop Extension (TALE) homeodomain-containing transcription factor playing crucial roles in organ pattering during embryogenesis, through the formation of nuclear complexes with other TALE class and/ ...

    Abstract Pre-B cell leukemia factor 1 (PBX1) is a Three Aminoacid Loop Extension (TALE) homeodomain-containing transcription factor playing crucial roles in organ pattering during embryogenesis, through the formation of nuclear complexes with other TALE class and/or homeobox proteins to regulate target genes. Its contribution to the development of several organs has been elucidated mainly through the study of murine knockout models. A crucial role for human development has been recently highlighted through the discovery of different
    Language English
    Publishing date 2024-02-09
    Publishing country Switzerland
    Document type Journal Article ; Review
    ZDB-ID 2737824-X
    ISSN 2296-634X
    ISSN 2296-634X
    DOI 10.3389/fcell.2024.1372873
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article ; Online: Hematopoietic Cells from Pluripotent Stem Cells: Hope and Promise for the Treatment of Inherited Blood Disorders.

    Rao, Ilaria / Crisafulli, Laura / Paulis, Marianna / Ficara, Francesca

    Cells

    2022  Volume 11, Issue 3

    Abstract: Inherited blood disorders comprise a large spectrum of diseases due to germline mutations in genes with key function in the hematopoietic system; they include immunodeficiencies, anemia or metabolic diseases. For most of them the only curative treatment ... ...

    Abstract Inherited blood disorders comprise a large spectrum of diseases due to germline mutations in genes with key function in the hematopoietic system; they include immunodeficiencies, anemia or metabolic diseases. For most of them the only curative treatment is bone marrow transplantation, a procedure associated to severe complications; other therapies include red blood cell and platelet transfusions, which are dependent on donor availability. An alternative option is gene therapy, in which the wild-type form of the mutated gene is delivered into autologous hematopoietic stem cells using viral vectors. A more recent therapeutic perspective is gene correction through CRISPR/Cas9-mediated gene editing, that overcomes safety concerns due to insertional mutagenesis and allows correction of base substitutions in large size genes difficult to incorporate into vectors. However, applying this technique to genomic disorders caused by large gene deletions is challenging. Chromosomal transplantation has been proposed as a solution, using a universal source of wild-type chromosomes as donor, and induced pluripotent stem cells (iPSCs) as acceptor. One of the obstacles to be addressed for translating PSC research into clinical practice is the still unsatisfactory differentiation into transplantable hematopoietic stem or mature cells. We provide an overview of the recent progresses in this field and discuss challenges and potential of iPSC-based therapies for the treatment of inherited blood disorders.
    MeSH term(s) Blood Coagulation Disorders, Inherited/blood ; Cell Differentiation ; Gene Editing/methods ; Genetic Therapy/methods ; Hematopoietic Stem Cells/metabolism ; Humans ; Pluripotent Stem Cells/metabolism
    Language English
    Publishing date 2022-02-05
    Publishing country Switzerland
    Document type Journal Article ; Review
    ZDB-ID 2661518-6
    ISSN 2073-4409 ; 2073-4409
    ISSN (online) 2073-4409
    ISSN 2073-4409
    DOI 10.3390/cells11030557
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article ; Online: Bone Marrow Niches and Tumour Cells: Lights and Shadows of a Mutual Relationship.

    Granata, Valentina / Crisafulli, Laura / Nastasi, Claudia / Ficara, Francesca / Sobacchi, Cristina

    Frontiers in immunology

    2022  Volume 13, Page(s) 884024

    Abstract: The bone marrow (BM) niche is the spatial structure within the intra-trabecular spaces of spongious bones and of the cavity of long bones where adult haematopoietic stem cells (HSCs) maintain their undifferentiated and cellular self-renewal state through ...

    Abstract The bone marrow (BM) niche is the spatial structure within the intra-trabecular spaces of spongious bones and of the cavity of long bones where adult haematopoietic stem cells (HSCs) maintain their undifferentiated and cellular self-renewal state through the intervention of vascular and nervous networks, metabolic pathways, transcriptional and epigenetic regulators, and humoral signals. Within the niche, HSCs interact with various cell types such as osteoblasts, endothelial cells, macrophages, and mesenchymal stromal cells (MSCs), which maintain HSCs in a quiescent state or sustain their proliferation, differentiation, and trafficking, depending on body needs. In physiological conditions, the BM niche permits the daily production of all the blood and immune cells and their admittance/ingress/progression into the bloodstream. However, disruption of this delicate microenvironment promotes the initiation and progression of malignancies such as those included in the spectrum of myeloid neoplasms, also favouring resistance to pharmacological therapies. Alterations in the MSC population and in the crosstalk with HSCs owing to tumour-derived factors contribute to the formation of a malignant niche. On the other hand, cells of the BM microenvironment cooperate in creating a unique milieu favouring metastasization of distant tumours into the bone. In this framework, the pro-tumorigenic role of MSCs is well-documented, and few evidence suggest also an anti-tumorigenic effect. Here we will review recent advances regarding the BM niche composition and functionality in normal and in malignant conditions, as well as the therapeutic implications of the interplay between its diverse cellular components and malignant cells.
    MeSH term(s) Bone Marrow/metabolism ; Endothelial Cells ; Hematopoietic Stem Cells/metabolism ; Mesenchymal Stem Cells/metabolism ; Stem Cell Niche
    Language English
    Publishing date 2022-05-06
    Publishing country Switzerland
    Document type Journal Article ; Review ; Research Support, Non-U.S. Gov't
    ZDB-ID 2606827-8
    ISSN 1664-3224 ; 1664-3224
    ISSN (online) 1664-3224
    ISSN 1664-3224
    DOI 10.3389/fimmu.2022.884024
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  6. Article: Emerging Insights into Molecular Mechanisms of Inflammation in Myelodysplastic Syndromes.

    Vallelonga, Veronica / Gandolfi, Francesco / Ficara, Francesca / Della Porta, Matteo Giovanni / Ghisletti, Serena

    Biomedicines

    2023  Volume 11, Issue 10

    Abstract: Inflammation impacts human hematopoiesis across physiologic and pathologic conditions, as signals derived from the bone marrow microenvironment, such as pro-inflammatory cytokines and chemokines, have been shown to alter hematopoietic stem cell (HSCs) ... ...

    Abstract Inflammation impacts human hematopoiesis across physiologic and pathologic conditions, as signals derived from the bone marrow microenvironment, such as pro-inflammatory cytokines and chemokines, have been shown to alter hematopoietic stem cell (HSCs) homeostasis. Dysregulated inflammation can skew HSC fate-related decisions, leading to aberrant hematopoiesis and potentially contributing to the pathogenesis of hematological disorders such as myelodysplastic syndromes (MDS). Recently, emerging studies have used single-cell sequencing and muti-omic approaches to investigate HSC cellular heterogeneity and gene expression in normal hematopoiesis as well as in myeloid malignancies. This review summarizes recent reports mechanistically dissecting the role of inflammatory signaling and innate immune response activation due to MDS progression. Furthermore, we highlight the growing importance of using multi-omic techniques, such as single-cell profiling and deconvolution methods, to unravel MDSs' heterogeneity. These approaches have provided valuable insights into the patterns of clonal evolution that drive MDS progression and have elucidated the impact of inflammation on the composition of the bone marrow immune microenvironment in MDS.
    Language English
    Publishing date 2023-09-23
    Publishing country Switzerland
    Document type Journal Article ; Review
    ZDB-ID 2720867-9
    ISSN 2227-9059
    ISSN 2227-9059
    DOI 10.3390/biomedicines11102613
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  7. Article ; Online: PBX1-directed stem cell transcriptional program drives tumor progression in myeloproliferative neoplasm.

    Muggeo, Sharon / Crisafulli, Laura / Uva, Paolo / Fontana, Elena / Ubezio, Marta / Morenghi, Emanuela / Colombo, Federico Simone / Rigoni, Rosita / Peano, Clelia / Vezzoni, Paolo / Della Porta, Matteo Giovanni / Villa, Anna / Ficara, Francesca

    Stem cell reports

    2021  Volume 16, Issue 11, Page(s) 2607–2616

    Abstract: PBX1 regulates the balance between self-renewal and differentiation of hematopoietic stem cells and maintains proto-oncogenic transcriptional pathways in early progenitors. Its increased expression was found in myeloproliferative neoplasm (MPN) patients ... ...

    Abstract PBX1 regulates the balance between self-renewal and differentiation of hematopoietic stem cells and maintains proto-oncogenic transcriptional pathways in early progenitors. Its increased expression was found in myeloproliferative neoplasm (MPN) patients bearing the JAK2
    MeSH term(s) Animals ; Disease Models, Animal ; Disease Progression ; Gene Expression Profiling/methods ; Gene Expression Regulation, Neoplastic ; Hematopoietic Stem Cells/metabolism ; Humans ; Mice, Knockout ; Mice, Transgenic ; Mutation ; Myeloproliferative Disorders/genetics ; Myeloproliferative Disorders/metabolism ; Myeloproliferative Disorders/pathology ; Neoplasms/genetics ; Neoplasms/metabolism ; Neoplasms/pathology ; Pre-B-Cell Leukemia Transcription Factor 1/genetics ; Pre-B-Cell Leukemia Transcription Factor 1/metabolism ; RNA-Seq/methods ; Signal Transduction/genetics ; Mice
    Chemical Substances Pbx1 protein, mouse ; Pre-B-Cell Leukemia Transcription Factor 1
    Language English
    Publishing date 2021-10-21
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2720528-9
    ISSN 2213-6711 ; 2213-6711
    ISSN (online) 2213-6711
    ISSN 2213-6711
    DOI 10.1016/j.stemcr.2021.09.016
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  8. Article ; Online: MicroRNA-127-3p controls murine hematopoietic stem cell maintenance by limiting differentiation.

    Crisafulli, Laura / Muggeo, Sharon / Uva, Paolo / Wang, Yulei / Iwasaki, Masayuki / Locatelli, Silvia / Anselmo, Achille / Colombo, Federico S / Carlo-Stella, Carmelo / Cleary, Michael L / Villa, Anna / Gentner, Bernhard / Ficara, Francesca

    Haematologica

    2019  Volume 104, Issue 9, Page(s) 1744–1755

    Abstract: The balance between self-renewal and differentiation is crucial to ensure the homeostasis of the hematopoietic system, and is a hallmark of hematopoietic stem cells. However, the underlying molecular pathways, including the role of micro-RNA, are not ... ...

    Abstract The balance between self-renewal and differentiation is crucial to ensure the homeostasis of the hematopoietic system, and is a hallmark of hematopoietic stem cells. However, the underlying molecular pathways, including the role of micro-RNA, are not completely understood. To assess the contribution of micro-RNA, we performed micro-RNA profiling of hematopoietic stem cells and their immediate downstream progeny multi-potent progenitors from wild-type control and Pbx1-conditional knockout mice, whose stem cells display a profound self-renewal defect. Unsupervised hierarchical cluster analysis separated stem cells from multi-potent progenitors, suggesting that micro-RNA might regulate the first transition step in the adult hematopoietic development. Notably, Pbx1-deficient and wild-type cells clustered separately, linking micro-RNAs to self-renewal impairment. Differential expression analysis of micro-RNA in the physiological stem cell-to-multi-potent progenitor transition and in Pbx1-deficient stem cells compared to control stem cells revealed miR-127-3p as the most differentially expressed. Furthermore, miR-127-3p was strongly stem cell-specific, being quickly down-regulated upon differentiation and not re-expressed further downstream in the bone marrow hematopoietic hierarchy. Inhibition of miR-127-3p function in Lineage-negative cells, achieved through a lentiviral-sponge vector, led to severe stem cell depletion, as assessed with serial transplantation assays. miR-127-3p-sponged stem cells displayed accelerated differentiation, which was uncoupled from proliferation, accounting for the observed stem cell reduction. miR-127-3p overexpression in Lineage-negative cells did not alter stem cell pool size, but gave rise to lymphopenia, likely due to lack of miR-127-3p physiological downregulation beyond the stem cell stage. Thus, tight regulation of miR-127-3p is crucial to preserve the self-renewing stem cell pool and homeostasis of the hematopoietic system.
    MeSH term(s) Animals ; Cell Differentiation ; Cell Lineage/genetics ; Cluster Analysis ; Crosses, Genetic ; Gene Expression Profiling ; Hematopoiesis ; Hematopoietic Stem Cells/cytology ; Homeostasis ; Humans ; K562 Cells ; Lentivirus/genetics ; Mice ; Mice, Knockout ; MicroRNAs/physiology ; Oxidative Stress ; Pre-B-Cell Leukemia Transcription Factor 1/metabolism
    Chemical Substances MicroRNAs ; Mirn127 microRNA, mouse ; Pbx1 protein, mouse ; Pre-B-Cell Leukemia Transcription Factor 1
    Language English
    Publishing date 2019-02-21
    Publishing country Italy
    Document type Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
    ZDB-ID 2333-4
    ISSN 1592-8721 ; 0017-6567 ; 0390-6078
    ISSN (online) 1592-8721
    ISSN 0017-6567 ; 0390-6078
    DOI 10.3324/haematol.2018.198499
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  9. Article ; Online: Chromosome Transplantation: Correction of the Chronic Granulomatous Disease Defect in Mouse Induced Pluripotent Stem Cells.

    Castelli, Alessandra / Susani, Lucia / Menale, Ciro / Muggeo, Sharon / Caldana, Elena / Strina, Dario / Cassani, Barbara / Recordati, Camilla / Scanziani, Eugenio / Ficara, Francesca / Villa, Anna / Vezzoni, Paolo / Paulis, Marianna

    Stem cells (Dayton, Ohio)

    2019  Volume 37, Issue 7, Page(s) 876–887

    Abstract: In spite of the progress in gene editing achieved in recent years, a subset of genetic diseases involving structural chromosome abnormalities, including aneuploidies, large deletions and complex rearrangements, cannot be treated with conventional gene ... ...

    Abstract In spite of the progress in gene editing achieved in recent years, a subset of genetic diseases involving structural chromosome abnormalities, including aneuploidies, large deletions and complex rearrangements, cannot be treated with conventional gene therapy approaches. We have previously devised a strategy, dubbed chromosome transplantation (CT), to replace an endogenous mutated chromosome with an exogenous normal one. To establish a proof of principle for our approach, we chose as disease model the chronic granulomatous disease (CGD), an X-linked severe immunodeficiency due to abnormalities in CYBB (GP91) gene, including large genomic deletions. We corrected the gene defect by CT in induced pluripotent stem cells (iPSCs) from a CGD male mouse model. The Hprt gene of the endogenous X chromosome was inactivated by CRISPR/Cas9 technology thus allowing the exploitation of the hypoxanthine-aminopterin-thymidine selection system to introduce a normal donor X chromosome by microcell-mediated chromosome transfer. X-transplanted clones were obtained, and diploid XY clones which spontaneously lost the endogenous X chromosome were isolated. These cells were differentiated toward the myeloid lineage, and functional granulocytes producing GP91 protein were obtained. We propose the CT approach to correct iPSCs from patients affected by other X-linked diseases with large deletions, whose treatment is still unsatisfactory. Stem Cells 2019;37:876-887.
    MeSH term(s) Aminopterin/metabolism ; Aminopterin/pharmacology ; Animals ; Base Sequence ; CRISPR-Cas Systems ; Cell Differentiation ; Chromosomes, Mammalian ; Clone Cells ; Culture Media/chemistry ; Disease Models, Animal ; Gene Editing/methods ; Genetic Therapy/methods ; Granulocytes/cytology ; Granulocytes/drug effects ; Granulocytes/metabolism ; Granulomatous Disease, Chronic/genetics ; Granulomatous Disease, Chronic/metabolism ; Granulomatous Disease, Chronic/pathology ; Granulomatous Disease, Chronic/therapy ; Humans ; Hypoxanthine/metabolism ; Hypoxanthine/pharmacology ; Hypoxanthine Phosphoribosyltransferase/deficiency ; Hypoxanthine Phosphoribosyltransferase/genetics ; Induced Pluripotent Stem Cells/drug effects ; Induced Pluripotent Stem Cells/metabolism ; Induced Pluripotent Stem Cells/pathology ; Male ; Mice ; NADPH Oxidase 2/deficiency ; NADPH Oxidase 2/genetics ; Proof of Concept Study ; Sequence Deletion ; Thioguanine/metabolism ; Thioguanine/pharmacology ; Thymidine/metabolism ; Thymidine/pharmacology ; X Chromosome/chemistry ; X Chromosome/metabolism
    Chemical Substances Culture Media ; Hypoxanthine (2TN51YD919) ; Cybb protein, mouse (EC 1.6.3.-) ; NADPH Oxidase 2 (EC 1.6.3.-) ; Hypoxanthine Phosphoribosyltransferase (EC 2.4.2.8) ; Thioguanine (FTK8U1GZNX) ; Aminopterin (JYB41CTM2Q) ; Thymidine (VC2W18DGKR)
    Language English
    Publishing date 2019-04-02
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 1143556-2
    ISSN 1549-4918 ; 1066-5099
    ISSN (online) 1549-4918
    ISSN 1066-5099
    DOI 10.1002/stem.3006
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  10. Article: Generation of an immunodeficient mouse model of tcirg1-deficient autosomal recessive osteopetrosis.

    Palagano, Eleonora / Muggeo, Sharon / Crisafulli, Laura / Tourkova, Irina L / Strina, Dario / Mantero, Stefano / Fontana, Elena / Locatelli, Silvia L / Monari, Marta / Morenghi, Emanuela / Carlo-Stella, Carmelo / Barnett, John B / Blair, Harry C / Vezzoni, Paolo / Villa, Anna / Sobacchi, Cristina / Ficara, Francesca

    Bone reports

    2020  Volume 12, Page(s) 100242

    Abstract: Background: Autosomal recessive osteopetrosis is a rare skeletal disorder with increased bone density due to a failure in osteoclast bone resorption. In most cases, the defect is cell-autonomous, and >50% of patients bear mutations in the : Methods: ... ...

    Abstract Background: Autosomal recessive osteopetrosis is a rare skeletal disorder with increased bone density due to a failure in osteoclast bone resorption. In most cases, the defect is cell-autonomous, and >50% of patients bear mutations in the
    Methods: We generated a new mouse model, which we named NSG oc/oc, presenting severe autosomal recessive osteopetrosis owing to the
    Results: We demonstrated that neonatal murine bone marrow transplantation rescued NSG oc/oc mice, in line with previous findings in the oc/oc parental strain and with evidence from clinical practice in humans. Importantly, we also demonstrated human cell chimerism in the bone marrow of NSG oc/oc mice transplanted with human CD34
    Conclusion: Our work paves the way to generating an improved xenograft model for
    Language English
    Publishing date 2020-01-07
    Publishing country United States
    Document type Journal Article
    ZDB-ID 2821774-3
    ISSN 2352-1872
    ISSN 2352-1872
    DOI 10.1016/j.bonr.2020.100242
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