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  1. Book ; Thesis: Rezeptor- und Transporterexpression für freie und konjugierte Steroidhormone bei normaler und gestörter Spermatogenese

    Fietz, Daniela

    (Edition Scientifique)

    2018  

    Author's details Daniela Fietz
    Series title Edition Scientifique
    Keywords Doktorarbeit ; Uni ; Wissenschaft ; Spermatogenese ; Steroidhormon ; Carrier-Proteine ; Steroidhormonrezeptor ; Genexpression
    Subject Steroidhormon ; Steroidrezeptor ; Steroid-Rezeptoren ; Transportproteine ; Translokatoren ; Membrantransportproteine ; Spermiogenese ; Spermienbildung
    Subject code 630
    Language German
    Size 239 Seiten, Illustrationen, Diagramme, 21 cm x 14.6 cm, 318 g
    Edition 1. Auflage
    Publisher VVB Laufersweiler Verlag
    Publishing place Gießen, Lahn
    Publishing country Germany
    Document type Book ; Thesis
    Thesis / German Habilitation thesis Habilitationsschrift, Universität Gießen, 2018
    HBZ-ID HT019744989
    ISBN 978-3-8359-6696-3 ; 3-8359-6696-0
    Database Catalogue ZB MED Medicine, Health

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    In stock of ZB MED Cologne/Königswinter

    Shelf markLoan statusLocation
    2018 A 1028 available for loan (reading room) Lesesaal EG

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  2. Article ; Online: In Situ Hybridization of Estrogen Receptors α and β in the Human Testis.

    Hartmann, Katja / Fietz, Daniela

    Methods in molecular biology (Clifton, N.J.)

    2022  Volume 2418, Page(s) 95–112

    Abstract: In situ hybridization (ISH) is an excellent method for detecting RNA in histological sections, both to detect gene expression and to assign gene expression to a distinct cell population. Therefore, ISH may be used in basic cell biology to detect the ... ...

    Abstract In situ hybridization (ISH) is an excellent method for detecting RNA in histological sections, both to detect gene expression and to assign gene expression to a distinct cell population. Therefore, ISH may be used in basic cell biology to detect the expression of certain genes within a tissue containing various cell populations. Here, we describe the detection and cellular localization of two estrogen receptors, both isoforms of the genomic estrogen receptor (ERα and ERβ) in the human testis.
    MeSH term(s) Estrogen Receptor alpha/genetics ; Estrogen Receptor alpha/metabolism ; Estrogen Receptor beta/genetics ; Estrogen Receptor beta/metabolism ; Humans ; In Situ Hybridization ; Male ; RNA, Messenger/genetics ; Testis/metabolism
    Chemical Substances ESR1 protein, human ; ESR2 protein, human ; Estrogen Receptor alpha ; Estrogen Receptor beta ; RNA, Messenger
    Language English
    Publishing date 2022-02-04
    Publishing country United States
    Document type Journal Article
    ISSN 1940-6029
    ISSN (online) 1940-6029
    DOI 10.1007/978-1-0716-1920-9_7
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Book ; Online ; Thesis: Rezeptor- und Transporterexpression für freie und konjugierte Steroidhormone bei normaler und gestörter Spermatogenese

    Fietz, Daniela [Verfasser]

    2019  

    Author's details Daniela Fietz
    Keywords Landwirtschaft, Veterinärmedizin ; Agriculture, Veterinary Science
    Subject code sg630
    Language German
    Publisher Universitätsbibliothek
    Publishing place Gießen
    Document type Book ; Online ; Thesis
    Database Digital theses on the web

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  4. Article ; Online: Modelling the Functions of Polo-Like Kinases in Mice and Their Applications as Cancer Targets with a Special Focus on Ovarian Cancer.

    Kressin, Monika / Fietz, Daniela / Becker, Sven / Strebhardt, Klaus

    Cells

    2021  Volume 10, Issue 5

    Abstract: Polo-like kinases (PLKs) belong to a five-membered family of highly conserved serine/threonine kinases (PLK1-5) that play differentiated and essential roles as key mitotic kinases and cell cycle regulators and with this in proliferation and cellular ... ...

    Abstract Polo-like kinases (PLKs) belong to a five-membered family of highly conserved serine/threonine kinases (PLK1-5) that play differentiated and essential roles as key mitotic kinases and cell cycle regulators and with this in proliferation and cellular growth. Besides, evidence is accumulating for complex and vital non-mitotic functions of PLKs. Dysregulation of PLKs is widely associated with tumorigenesis and by this, PLKs have gained increasing significance as attractive targets in cancer with diagnostic, prognostic and therapeutic potential. PLK1 has proved to have strong clinical relevance as it was found to be over-expressed in different cancer types and linked to poor patient prognosis. Targeting the diverse functions of PLKs (tumor suppressor, oncogenic) are currently at the center of numerous investigations in particular with the inhibition of PLK1 and PLK4, respectively in multiple cancer trials. Functions of PLKs and the effects of their inhibition have been extensively studied in cancer cell culture models but information is rare on how these drugs affect benign tissues and organs. As a step further towards clinical application as cancer targets, mouse models therefore play a central role. Modelling PLK function in animal models, e.g., by gene disruption or by treatment with small molecule PLK inhibitors offers promising possibilities to unveil the biological significance of PLKs in cancer maintenance and progression and give important information on PLKs' applicability as cancer targets. In this review we aim at summarizing the approaches of modelling PLK function in mice so far with a special glimpse on the significance of PLKs in ovarian cancer and of orthotopic cancer models used in this fatal malignancy.
    MeSH term(s) Animals ; Carcinogenesis ; Carcinoma, Ovarian Epithelial/genetics ; Cell Cycle Proteins/metabolism ; Cell Proliferation ; Disease Models, Animal ; Disease Progression ; Female ; Gene Silencing ; Genes, Tumor Suppressor ; Humans ; Mice ; Mitosis ; Ovarian Neoplasms/drug therapy ; Ovarian Neoplasms/enzymology ; Protein Serine-Threonine Kinases/metabolism ; Proto-Oncogene Proteins/metabolism ; Tumor Suppressor Proteins/metabolism ; Polo-Like Kinase 1
    Chemical Substances Cell Cycle Proteins ; Proto-Oncogene Proteins ; Tumor Suppressor Proteins ; PLK3 protein, human (EC 2.7.1.-) ; PLK4 protein, human (EC 2.7.1.-) ; PLK2 protein, human (EC 2.7.11.-) ; Protein Serine-Threonine Kinases (EC 2.7.11.1)
    Language English
    Publishing date 2021-05-12
    Publishing country Switzerland
    Document type Journal Article ; Review
    ZDB-ID 2661518-6
    ISSN 2073-4409 ; 2073-4409
    ISSN (online) 2073-4409
    ISSN 2073-4409
    DOI 10.3390/cells10051176
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article ; Online: Effects of a Sertoli cell-specific knockout of Connexin43 on maturation and proliferation of postnatal Sertoli cells.

    Hüneke, Hanna / Langeheine, Marion / Rode, Kristina / Jung, Klaus / Pilatz, Adrian / Fietz, Daniela / Kliesch, Sabine / Brehm, Ralph

    Differentiation; research in biological diversity

    2023  Volume 134, Page(s) 31–51

    Abstract: Adult male Sertoli cell-specific Connexin43 knockout mice (SCCx43KO) exhibit higher Sertoli cell (SC) numbers per seminiferous tubule compared to their wild type (WT) littermates. Thus, deletion of this testicular gap junction protein seems to affect the ...

    Abstract Adult male Sertoli cell-specific Connexin43 knockout mice (SCCx43KO) exhibit higher Sertoli cell (SC) numbers per seminiferous tubule compared to their wild type (WT) littermates. Thus, deletion of this testicular gap junction protein seems to affect the proliferative potential and differentiation of "younger" SC. Although SC have so far mostly been characterised as postmitotic cells that cease to divide and become an adult, terminally differentiated cell population at around puberty, there is rising evidence that there exist exceptions from this for a very long time accepted paradigm. Aim of this study was to investigate postnatal SC development and to figure out underlying causes for observed higher SC numbers in adult KO mice. Therefore, the amount of SC mitotic figures was compared, resulting in slightly more and prolonged detection of SC mitotic figures in KO mice compared to WT. SC counting per tubular cross section revealed significantly different time curves, and comparing proliferation rates using Bromodesoxyuridine and Sox9 showed higher proliferation rates in 8-day old KO mice. SC proliferation was further investigated by Ki67 immunohistochemistry. SC in KO mice displayed a delayed initiation of cell-cycle-inhibitor p27
    MeSH term(s) Male ; Animals ; Mice ; Sertoli Cells ; Connexin 43/genetics ; Connexin 43/metabolism ; Ki-67 Antigen/genetics ; Testis ; Mice, Knockout ; Connexins/metabolism ; Cell Proliferation/genetics ; Spermatogenesis
    Chemical Substances Connexin 43 ; Ki-67 Antigen ; Connexins
    Language English
    Publishing date 2023-09-25
    Publishing country England
    Document type Journal Article
    ZDB-ID 184540-8
    ISSN 1432-0436 ; 0301-4681
    ISSN (online) 1432-0436
    ISSN 0301-4681
    DOI 10.1016/j.diff.2023.09.002
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 1170: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (1.OG)
    ab Jg. 2022: Lesesaal (EG)

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  6. Article: Proteomic biomarkers in seminal plasma as predictors of reproductive potential in azoospermic men.

    Fietz, Daniela / Sgaier, Raouda / O'Donnell, Liza / Stanton, Peter G / Dagley, Laura F / Webb, Andrew I / Schuppe, Hans-Christian / Diemer, Thorsten / Pilatz, Adrian

    Frontiers in endocrinology

    2024  Volume 15, Page(s) 1327800

    Abstract: Introduction: Azoospermia, characterized by an absence of sperm in the ejaculate, represents the most severe form of male infertility. While surgical sperm retrieval in obstructive azoospermia (OA) is successful in the majority of cases, patients with ... ...

    Abstract Introduction: Azoospermia, characterized by an absence of sperm in the ejaculate, represents the most severe form of male infertility. While surgical sperm retrieval in obstructive azoospermia (OA) is successful in the majority of cases, patients with non-obstructive azoospermia (NOA) show retrieval rates of only about 50% and thus frequently have unnecessary surgery. Surgical intervention could be avoided if patients without preserved spermatogenesis are identified preoperatively. This prospective study aimed to discover biomarkers in seminal plasma that could be employed for a non-invasive differential diagnosis of OA/NOA in order to rationalize surgery recommendations and improve success rates.
    Methods: All patients signed written informed consent, underwent comprehensive andrological evaluation, received human genetics to exclude relevant pathologies, and patients with azoospermia underwent surgical sperm retrieval. Using label-free LC-MS/MS, we compared the proteomes of seminal plasma samples from fertile men (healthy controls (HC), n=8) and infertile men diagnosed with 1) OA (n=7), 2) NOA with successful sperm retrieval (mixed testicular atrophy (MTA), n=8), and 3) NOA without sperm retrieval (Sertoli cell-only phenotype (SCO), n=7). Relative abundance changes of two candidate markers of sperm retrieval, HSPA2 and LDHC, were confirmed by Western Blot.
    Results: We found the protein expression levels of 42 proteins to be significantly down-regulated (p ≤ 0.05) in seminal plasma from SCO NOA patients relative to HC whereas only one protein was down-regulated in seminal plasma from MTA patients. Analysis of tissue and cell expression suggested that the testis-specific proteins LDHC, PGK2, DPEP3, and germ-cell enriched heat-shock proteins HSPA2 and HSPA4L are promising biomarkers of spermatogenic function. Western blotting revealed a significantly lower abundance of LDHC and HSPA2 in the seminal plasma of men with NOA (SCO and MTA) compared to controls.
    Discussion: The results indicate that certain testis-specific proteins when measured in seminal plasma, could serve as indicators of the presence of sperm in the testis and predict the success of sperm retrieval. Used in conjunction with conventional clinical assessments, these proteomic biomarkers may assist in the non-invasive diagnosis of idiopathic male infertility.
    MeSH term(s) Humans ; Male ; Azoospermia/metabolism ; Azoospermia/diagnosis ; Semen/metabolism ; Semen/chemistry ; Biomarkers/metabolism ; Biomarkers/analysis ; Biomarkers/blood ; Adult ; Proteomics/methods ; Prospective Studies ; Sperm Retrieval ; Case-Control Studies ; Spermatogenesis/physiology
    Chemical Substances Biomarkers
    Language English
    Publishing date 2024-04-09
    Publishing country Switzerland
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2592084-4
    ISSN 1664-2392
    ISSN 1664-2392
    DOI 10.3389/fendo.2024.1327800
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  7. Article ; Online: Task force veterinary anatomy: joint efforts of the five German veterinary schools to ensure education during the COVID-19 pandemic.

    Bernigau, Dora / Bahramsoltani, Mahtab / Corte, Giuliano Mario / Reese, Sven / Pfarrer, Christiane / Fietz, Daniela

    GMS journal for medical education

    2021  Volume 38, Issue 5, Page(s) Doc87

    Abstract: At the start of the COVID-pandemic in March 2020, the Institutes of Veterinary Anatomy of the five German educational institutions were confronted with the challenge of digitalising all lectures for the second and fourth semesters of veterinary students. ...

    Abstract At the start of the COVID-pandemic in March 2020, the Institutes of Veterinary Anatomy of the five German educational institutions were confronted with the challenge of digitalising all lectures for the second and fourth semesters of veterinary students. After an online kick-off event and a preliminary status quo meeting, available digital teaching material was exchanged for students to stream from learning platforms. Lectures were either synchronized or made available as audio recordings and connotated slides on the learning platforms. Fortunately, digital microscopic slides had already been in use, which made it easy for students to access them. Dissection exercises mostly consisted of self-study, using instructive videos and interactive exercises. In the second half of the semester, four of the educational institutions were able to offer a restricted number of in-person gross anatomy classes under reinforced conditions. Success monitoring took place online through different formats, and partially on a voluntary basis, via the learning platforms. Although the past two semesters had to almost exclusively take place online due to the unprecedented circumstances, and joint efforts of the five veterinary institutions, there is a general consensus that the practical education in anatomy, histology and embryology is essential to veterinary students. In fact, it is the only way they can obtain the necessary skills to successfully complete the rest of their degree.
    MeSH term(s) Anatomy/education ; Audiovisual Aids ; COVID-19 ; Digital Technology ; Dissection ; Education, Distance ; Education, Veterinary/methods ; Germany ; Humans ; Internet ; Microscopy ; Pandemics ; Physical Distancing ; Professional Competence ; Schools, Veterinary ; Students ; Teaching ; Universities ; Veterinary Medicine
    Language English
    Publishing date 2021-06-15
    Publishing country Germany
    Document type Journal Article
    ISSN 2366-5017
    ISSN (online) 2366-5017
    DOI 10.3205/zma001483
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  8. Article ; Online: The changing landscape of immune cells in the fetal mouse testis.

    Hosseini, Samira / Moody, Sarah C / Fietz, Daniela / Indumathy, Sivanjah / Schuppe, Hans-Christian / Hedger, Mark P / Loveland, Kate L

    Histochemistry and cell biology

    2022  Volume 158, Issue 4, Page(s) 345–368

    Abstract: Fetal testis growth involves cell influx and extensive remodeling. Immediately after sex determination in mouse, macrophages enable normal cord formation and removal of inappropriately positioned cells. This study provides new information about ... ...

    Abstract Fetal testis growth involves cell influx and extensive remodeling. Immediately after sex determination in mouse, macrophages enable normal cord formation and removal of inappropriately positioned cells. This study provides new information about macrophages and other immune cells after cord formation in fetal testes, including their density, distribution, and close cellular contacts. C57BL6J mouse testes from embryonic day (E) 13.5 to birth (post-natal day 0; PND0), were examined using immunofluorescence, immunohistochemistry, and RT-qPCR to identify macrophages (F4/80, CD206, MHCII), T cells (CD3), granulocytes/neutrophils (Ly6G), and germ cells (DDX4). F4/80
    MeSH term(s) Animals ; Fetal Development ; Germ Cells ; Macrophages ; Male ; Mice ; Morphogenesis ; Testis
    Language English
    Publishing date 2022-07-12
    Publishing country Germany
    Document type Journal Article
    ZDB-ID 1222930-1
    ISSN 1432-119X ; 0301-5564 ; 0948-6143
    ISSN (online) 1432-119X
    ISSN 0301-5564 ; 0948-6143
    DOI 10.1007/s00418-022-02129-6
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    In stock of ZB MED Cologne/Königswinter

    Ub I Zs.94/a: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular
    ab Jg. 2022: Lesesaal (EG)

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  9. Book ; Online ; Thesis: Molekularbiologische Untersuchung des CAG-Repeats des humanen Androgenrezeptors und Korrelation mit der Histologie der Spermatogenese - Ist das CAG-Repeat ein molekularer Marker für männliche Infertilität?

    Fietz, Daniela [Verfasser]

    2012  

    Author's details Daniela Christa Fietz
    Keywords Landwirtschaft, Veterinärmedizin ; Agriculture, Veterinary Science
    Subject code sg630
    Language German
    Publisher Universitätsbibliothek
    Publishing place Gießen
    Document type Book ; Online ; Thesis
    Database Digital theses on the web

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  10. Book ; Online ; Thesis: Molekularbiologische Untersuchung des CAG-Repeats des humanen Androgenrezeptors und Korrelation mit der Histologie der Spermatogenese - Ist das CAG-Repeat ein molekularer Marker für männliche Infertilität?

    Fietz, Daniela Christa

    2012  

    Author's details Daniela Christa Fietz
    Language German
    Size Online-Ressource
    Publisher Universitätsbibliothek
    Publishing place Gießen
    Document type Book ; Online ; Thesis
    Thesis / German Habilitation thesis Gießen, Justus-Liebig-Universität, Diss., 2011
    Database Former special subject collection: coastal and deep sea fishing

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