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  1. Article ; Online: Detecting PTP Protein-Protein Interactions by Fluorescent Immunoprecipitation Analysis (FIPA).

    Kruglova, Natalia / Filatov, Alexander

    Methods in molecular biology (Clifton, N.J.)

    2023  Volume 2743, Page(s) 181–194

    Abstract: Identifying protein-protein interactions is crucial for revealing protein functions and characterizing cellular processes. Manipulating PPIs has become widespread in treating human diseases such as cancer, autoimmunity, and infections. It has been ... ...

    Abstract Identifying protein-protein interactions is crucial for revealing protein functions and characterizing cellular processes. Manipulating PPIs has become widespread in treating human diseases such as cancer, autoimmunity, and infections. It has been recently applied to the regulation of protein tyrosine phosphatases (PTPs) previously considered undruggable. A broad panel of methods is available for studying PPIs. To complement the existing toolkit, we developed a simple method called fluorescent immunoprecipitation analysis (FIPA). This method is based on coimmunoprecipitation followed by protein gel electrophoresis and fluorescent imaging to visualize components of a protein complex simultaneously on a gel. The FIPA allows the detection of proteins expressed under native conditions and is compatible with mass spectrometry identification of protein bands.
    MeSH term(s) Humans ; Autoimmunity ; Coloring Agents ; Immunoprecipitation ; Mass Spectrometry
    Chemical Substances Coloring Agents
    Language English
    Publishing date 2023-12-26
    Publishing country United States
    Document type Journal Article
    ISSN 1940-6029
    ISSN (online) 1940-6029
    DOI 10.1007/978-1-0716-3569-8_12
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  2. Article ; Online: Structure and Magnetic Properties of Pseudo-1D Chromium Thiolate Coordination Polymers.

    Ritchhart, Andrew / Filatov, Alexander S / Jeon, Ie-Rang / Anderson, John S

    Inorganic chemistry

    2023  Volume 62, Issue 6, Page(s) 2817–2825

    Abstract: The synthesis, structure, and magnetic properties of two novel, pseudo-one-dimensional (1D) chromium thiolate coordination polymers (CPs), CrBTT and ... ...

    Abstract The synthesis, structure, and magnetic properties of two novel, pseudo-one-dimensional (1D) chromium thiolate coordination polymers (CPs), CrBTT and Cr
    Language English
    Publishing date 2023-02-02
    Publishing country United States
    Document type Journal Article
    ZDB-ID 1484438-2
    ISSN 1520-510X ; 0020-1669
    ISSN (online) 1520-510X
    ISSN 0020-1669
    DOI 10.1021/acs.inorgchem.2c03991
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  3. Article: Cobalt-Catalyzed Hydrogenation Reactions Enabled by Ligand-Based Storage of Dihydrogen.

    Anferov, Sophie W / Filatov, Alexander S / Anderson, John S

    ACS catalysis

    2022  Volume 12, Issue 16, Page(s) 9933–9943

    Abstract: The use of supporting ligands that can store either protons or electrons has emerged as a powerful strategy in catalysis. While these strategies are potent individually, natural systems mediate remarkable transformations by combining the storage of both ... ...

    Abstract The use of supporting ligands that can store either protons or electrons has emerged as a powerful strategy in catalysis. While these strategies are potent individually, natural systems mediate remarkable transformations by combining the storage of both protons and electrons in the secondary coordination sphere. As such, there has been recent interest in using this strategy to enable fundamentally different transformations. Furthermore, outsourcing H-atom or hydrogen storage to ancillary ligands can also enable alternative mechanistic pathways and thereby selectivity. Here, we describe the application of this strategy to facilitate radical reactivity in Co-based hydrogenation catalysis. Metalation of previously reported dihydrazonopyrrole ligands with Co results in paramagnetic complexes, which are best described as having Co(II) oxidation states. These complexes catalytically hydrogenate olefins with low catalyst loadings under mild conditions (1 atm H
    Language English
    Publishing date 2022-08-01
    Publishing country United States
    Document type Journal Article
    ISSN 2155-5435
    ISSN 2155-5435
    DOI 10.1021/acscatal.2c02467
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  4. Article ; Online: A Synthetic Cycle for Heteroarene Synthesis by Nitride Insertion.

    Kelly, Patrick Q / Filatov, Alexander S / Levin, Mark D

    Angewandte Chemie (International ed. in English)

    2022  Volume 61, Issue 46, Page(s) e202213041

    Abstract: Recent interest in skeletal editing necessitates the continued development of reagent classes with the ability to transfer single atoms. Terminal transition metal nitrides hold immense promise for single-atom transfer, though their use in organic ... ...

    Abstract Recent interest in skeletal editing necessitates the continued development of reagent classes with the ability to transfer single atoms. Terminal transition metal nitrides hold immense promise for single-atom transfer, though their use in organic synthesis has so far been limited. Here we demonstrate a synthetic cycle with associated detailed mechanistic studies that primes the development of terminal transition metal nitrides as valuable single-atom transfer reagents. Specifically, we show [cis-terpyOsNCl
    Language English
    Publishing date 2022-10-18
    Publishing country Germany
    Document type Journal Article
    ZDB-ID 2011836-3
    ISSN 1521-3773 ; 1433-7851
    ISSN (online) 1521-3773
    ISSN 1433-7851
    DOI 10.1002/anie.202213041
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  5. Article ; Online: Research of high-performance harvesting and transport equipment

    Kokieva Galia / Dondokov Yrii / Stepanova Daria / Stroyev Alexei / Filatov Alexander

    E3S Web of Conferences, Vol 273, p

    2021  Volume 07005

    Abstract: The question of applying the most progressive technologies of cleaning work of efficient systems of machines is of paramount importance. In solving tasks to ensure the sustainable development of agricultural production, reliable supply of the population ... ...

    Abstract The question of applying the most progressive technologies of cleaning work of efficient systems of machines is of paramount importance. In solving tasks to ensure the sustainable development of agricultural production, reliable supply of the population of the country, food in food is an important place to strengthen the material and technical base of the agro-industrial complex, its targeted technical re-equipment. In this regard, the goal of the work is to study the rational method of increasing the performance of the screw screw conveyor, allowing you to create a small-sized structure, is an increase in its revolutions. The article provides research in the method of increasing the performance of the screw screw conveyor, allowing you to create a small-sized design by increasing its revolutions. The technology of the work of conveyors-grab trucks was described, the work mechanism was investigated and the disadvantages of work were described, namely, in defective providing the possibility of grain transfer over long distances along the spatial track or complicates the technological scheme. Such an organization of harvesting on the scale of the country involves the creation and implementation of several systems of high-performance cleaning and transport equipment primarily in the main grain areas.
    Keywords Environmental sciences ; GE1-350
    Subject code 650
    Language English
    Publishing date 2021-01-01T00:00:00Z
    Publisher EDP Sciences
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  6. Article ; Online: Dissection of Antibody Responses of Gam-COVID-Vac-Vaccinated Subjects Suggests Involvement of Epitopes Outside RBD in SARS-CoV-2 Neutralization.

    Byazrova, Maria / Gattinger, Pia / Astakhova, Ekaterina / Hofer, Gerhard / Khaitov, Musa / Filatov, Alexander / Valenta, Rudolf

    International journal of molecular sciences

    2023  Volume 24, Issue 6

    Abstract: Millions of people have been vaccinated with Gam-COVID-Vac but fine specificities of induced antibodies have not been fully studied. Plasma from 12 naïve and 10 coronavirus disease 2019 (COVID-19) convalescent subjects was obtained before and after two ... ...

    Abstract Millions of people have been vaccinated with Gam-COVID-Vac but fine specificities of induced antibodies have not been fully studied. Plasma from 12 naïve and 10 coronavirus disease 2019 (COVID-19) convalescent subjects was obtained before and after two immunizations with Gam-COVID-Vac. Antibody reactivity in the plasma samples (n = 44) was studied on a panel of micro-arrayed recombinant folded and unfolded severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) proteins and 46 peptides spanning the spike protein (S) and by immunoglobulin G (IgG) subclass enzyme-linked immunosorbent assay (ELISA). The ability of Gam-COVID-Vac-induced antibodies to inhibit binding of the receptor-binding domain (RBD) to its receptor angiotensin converting enzyme 2 (ACE2) was investigated in a molecular interaction assay (MIA). The virus-neutralizing capacity of antibodies was studied by the pseudo-typed virus neutralization test (pVNT) for Wuhan-Hu-1 and Omicron. We found that Gam-COVID-Vac vaccination induced significant increases of IgG
    MeSH term(s) Humans ; SARS-CoV-2/metabolism ; COVID-19 ; Epitopes ; Antibodies, Neutralizing ; Antibodies, Viral ; Protein Subunits ; Spike Glycoprotein, Coronavirus/metabolism ; Antibody Formation ; Immunoglobulin G
    Chemical Substances Epitopes ; Antibodies, Neutralizing ; Antibodies, Viral ; Protein Subunits ; Spike Glycoprotein, Coronavirus ; Immunoglobulin G ; spike protein, SARS-CoV-2
    Language English
    Publishing date 2023-03-07
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2019364-6
    ISSN 1422-0067 ; 1422-0067 ; 1661-6596
    ISSN (online) 1422-0067
    ISSN 1422-0067 ; 1661-6596
    DOI 10.3390/ijms24065104
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  7. Article ; Online: Catalytic, Spectroscopic, and Theoretical Studies of Fe

    Jiang, Ningxin / Darù, Andrea / Kunstelj, Špela / Vitillo, Jenny G / Czaikowski, Maia E / Filatov, Alexander S / Wuttig, Anna / Gagliardi, Laura / Anderson, John S

    Journal of the American Chemical Society

    2024  Volume 146, Issue 17, Page(s) 12243–12252

    Abstract: Iron-sulfur clusters play essential roles in biological systems, and thus synthetic [ ... ...

    Abstract Iron-sulfur clusters play essential roles in biological systems, and thus synthetic [Fe
    Language English
    Publishing date 2024-04-23
    Publishing country United States
    Document type Journal Article
    ZDB-ID 3155-0
    ISSN 1520-5126 ; 0002-7863
    ISSN (online) 1520-5126
    ISSN 0002-7863
    DOI 10.1021/jacs.4c03726
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    In stock of ZB MED Bonn / Germany

    Z 4' 55/32: Show issues
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  8. Article ; Online: T cell receptor signaling results in ERK-dependent Ser163 phosphorylation of lymphocyte phosphatase-associated phosphoprotein.

    Kruglova, Natalia / Filatov, Alexander

    Biochemical and biophysical research communications

    2019  Volume 519, Issue 3, Page(s) 559–565

    Abstract: Lymphocyte phosphatase-associated phosphoprotein (LPAP) is a transmembrane protein tightly associated with the phosphatase CD45, which regulates antigen specific lymphocyte activation. Although LPAP is positioned in close proximity to key signaling ... ...

    Abstract Lymphocyte phosphatase-associated phosphoprotein (LPAP) is a transmembrane protein tightly associated with the phosphatase CD45, which regulates antigen specific lymphocyte activation. Although LPAP is positioned in close proximity to key signaling molecules, its function remains unknown. In this study, we investigated signaling pathways involved in LPAP phosphorylation. Using phosphospecific antibodies generated in our laboratory, we analyzed changes in LPAP phosphorylation in response to various stimuli. Cross-linking with antibodies against TCR or BCR, as well as ionophores and Thapsigargin, resulted in rapid dephosphorylation at Ser172 and Ser99 followed by phosphorylation at Ser163. A panel of inhibitors allowed us to show that PMA and TCR cross-linkage engages the MEK-ERK pathway to drive phosphorylation of LPAP at Ser163. The ERK1/2 kinase was the most distal element in the cascade, which when inhibited prevented changes in LPAP phosphorylation. Supporting this, we found that ERK1 is capable of phosphorylating LPAP at Ser163 in vitro. Although the functional role of this event is yet to be revealed, we provide evidence for a new ERK1/2 target in lymphocytes, namely LPAP, representing a potential regulatory mechanism in the signaling cascade.
    MeSH term(s) Cells, Cultured ; Extracellular Signal-Regulated MAP Kinases/metabolism ; Humans ; Intracellular Signaling Peptides and Proteins/metabolism ; Lymphocyte Activation ; Lymphocytes/metabolism ; Membrane Proteins/metabolism ; Phosphorylation ; Receptors, Antigen, T-Cell/metabolism ; Serine/metabolism ; Signal Transduction
    Chemical Substances Intracellular Signaling Peptides and Proteins ; Membrane Proteins ; PTPRCAP protein, human ; Receptors, Antigen, T-Cell ; Serine (452VLY9402) ; Extracellular Signal-Regulated MAP Kinases (EC 2.7.11.24)
    Language English
    Publishing date 2019-09-16
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 205723-2
    ISSN 1090-2104 ; 0006-291X ; 0006-291X
    ISSN (online) 1090-2104 ; 0006-291X
    ISSN 0006-291X
    DOI 10.1016/j.bbrc.2019.09.041
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    In stock of ZB MED Cologne/Königswinter

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  9. Article ; Online: The increasing concentration at industrial markets

    Filatov Alexander / Makolskaya Yana

    SHS Web of Conferences, Vol 35, p

    the social welfare maximization and possible risks

    2017  Volume 01073

    Abstract: The paper considers impact of entry barriers on the social welfare. Despite the common opinion that entry barriers are always bad, the excessive number of firms means, all pros aside, duplicated fixed costs. It is shown that the socially effective number ...

    Abstract The paper considers impact of entry barriers on the social welfare. Despite the common opinion that entry barriers are always bad, the excessive number of firms means, all pros aside, duplicated fixed costs. It is shown that the socially effective number of firms is smaller than the equilibrium one for the wide spectre of demand and cost functions, and also for different strategies of companies’ behavior. This proposition is satisfied for the homogeneous product markets where output of each company decreases when the number of firms increases, and competition gets stronger. But there is the considerable danger of the increasing probability of collusion in a situation of number of firms limitation. We show that collusion is less dangerous than duplicated fixed costs if the gap between the «choke price» and marginal costs is less than a certain critical value connected with the share of fixed costs. The empirical research on the base of the financial statistics of the biggest world corporations of different industries is carried out.
    Keywords Social Sciences ; H
    Subject code 338
    Language English
    Publishing date 2017-01-01T00:00:00Z
    Publisher EDP Sciences
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  10. Article ; Online: Model of monopolistic competition with heterogeneous labor

    Filatov Alexander / Sokolovsky Yury

    SHS Web of Conferences, Vol 35, p

    2017  Volume 01071

    Abstract: The paper presents a tool for modelling monopolistic competition markets, based on Dixit-Stiglitz ideology but taking into account heterogeneity at labor market. We analyse several modifications of a two-sector general equilibrium model. In the basic one ...

    Abstract The paper presents a tool for modelling monopolistic competition markets, based on Dixit-Stiglitz ideology but taking into account heterogeneity at labor market. We analyse several modifications of a two-sector general equilibrium model. In the basic one with two levels of workers qualification their shares are determined endogenously on the base of comparison between the higher wage of the skilled worker and heterogeneous education costs, also taking into account the labor mobility between the manufacture and agriculture sector. The model is generalized for the case of continuous distribution of labor qualification. The impact of the model parameters (ratio of fixed and variable costs, market size, heterogeneity in productivity, elasticity of substitution, etc.) on the obtained equilibrium prices, quantities, wages, number and size of firms, social welfare is investigated.
    Keywords Social Sciences ; H
    Language English
    Publishing date 2017-01-01T00:00:00Z
    Publisher EDP Sciences
    Document type Article ; Online
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