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  1. Article ; Online: Identification of Torquetenovirus Species in Patients with Kawasaki Disease Using a Newly Developed Species-Specific PCR Method.

    Spezia, Pietro Giorgio / Filippini, Fabio / Nagao, Yoshiro / Sano, Tetsuya / Ishida, Takafumi / Maggi, Fabrizio

    International journal of molecular sciences

    2023  Volume 24, Issue 10

    Abstract: A next-generation sequencing (NGS) study identified a very high viral load of Torquetenovirus (TTV) in KD patients. We aimed to evaluate the feasibility of a newly developed quantitative species-specific TTV-PCR (ssTTV-PCR) method to identify the ... ...

    Abstract A next-generation sequencing (NGS) study identified a very high viral load of Torquetenovirus (TTV) in KD patients. We aimed to evaluate the feasibility of a newly developed quantitative species-specific TTV-PCR (ssTTV-PCR) method to identify the etiology of KD. We applied ssTTV-PCR to samples collected from 11 KD patients and 22 matched control subjects who participated in our previous prospective study. We used the NGS dataset from the previous study to validate ssTTV-PCR. The TTV loads in whole blood and nasopharyngeal aspirates correlated highly (Spearman's R = 0.8931,
    MeSH term(s) Torque teno virus/genetics ; Torque teno virus/isolation & purification ; Mucocutaneous Lymph Node Syndrome/virology ; Polymerase Chain Reaction/methods ; High-Throughput Nucleotide Sequencing ; Datasets as Topic ; Humans ; Male ; Female ; Infant ; Child, Preschool ; Child ; Prospective Studies ; DNA, Viral/genetics ; DNA, Viral/isolation & purification ; DNA Virus Infections/virology
    Chemical Substances DNA, Viral
    Language English
    Publishing date 2023-05-12
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2019364-6
    ISSN 1422-0067 ; 1422-0067 ; 1661-6596
    ISSN (online) 1422-0067
    ISSN 1422-0067 ; 1661-6596
    DOI 10.3390/ijms24108674
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: Torque Teno Virus (TTV) in Renal Transplant Recipients: Species Diversity and Variability.

    Reyes, Noelia Soledad / Spezia, Pietro Giorgio / Jara, Raquel / Filippini, Fabio / Boccia, Natalia / García, Gonzalo / Hermida, Eliana / Poletta, Fernando Adrian / Pistello, Mauro / Laham, Gustavo / Maggi, Fabrizio / Echavarria, Marcela

    Viruses

    2024  Volume 16, Issue 3

    Abstract: Torque Teno Virus (TTV) is a nonpathogenic and ubiquitous ssDNA virus, a member of ... ...

    Abstract Torque Teno Virus (TTV) is a nonpathogenic and ubiquitous ssDNA virus, a member of the
    MeSH term(s) Humans ; Torque teno virus/genetics ; Kidney Transplantation/adverse effects ; Phylogeny ; DNA Virus Infections ; Transplant Recipients ; Viral Load ; DNA, Viral/genetics
    Chemical Substances DNA, Viral
    Language English
    Publishing date 2024-03-11
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2516098-9
    ISSN 1999-4915 ; 1999-4915
    ISSN (online) 1999-4915
    ISSN 1999-4915
    DOI 10.3390/v16030432
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article ; Online: Viral load of Torquetenovirus correlates with Sano's score and levels of total bilirubin and aspartate aminotransferase in Kawasaki disease.

    Spezia, Pietro Giorgio / Matsudaira, Kazunari / Filippini, Fabio / Miyamura, Takako / Okada, Keiko / Nagao, Yoshiro / Ishida, Takafumi / Sano, Tetsuya / Pistello, Mauro / Maggi, Fabrizio / Hara, Junichi

    Scientific reports

    2023  Volume 13, Issue 1, Page(s) 18033

    Abstract: Cause of Kawasaki disease (KD) is unknown. KD is often resistant to treatment with intravenous immunoglobulin (IVIG). Sano's score, which is derived from total bilirubin (TBIL), aspartate aminotransferase (AST) and C-reactive protein (CRP), is predictive ...

    Abstract Cause of Kawasaki disease (KD) is unknown. KD is often resistant to treatment with intravenous immunoglobulin (IVIG). Sano's score, which is derived from total bilirubin (TBIL), aspartate aminotransferase (AST) and C-reactive protein (CRP), is predictive of IVIG resistance in Japan. A recent study reported that Torquetenovirus (TTV), especially TTV7, was present at a high viral load in the patients with KD. We used PCR to quantify TTV load and amplicon next generation sequencing to detect individual TTV species. We used serum samples that were collected between 2002 and 2005 from 57 Japanese KD patients before IVIG treatment. Correlations between TTV load and Sano's score, the biomarkers that constitute this score, and IVIG resistance were examined. TTV load was positively correlated with Sano's score (P = 0.0248), TBIL (P = 0.0004), and AST (P = 0.0385), but not with CRP (P = 0.6178). TTV load was marginally correlated with IVIG resistance (P = 0.1544). Presence of TTV7 was correlated with total TTV load significantly (P = 0.0231). The correlations between biomarkers for KD and TTV load suggested that TTV may play a role in the pathophysiology of KD. We hypothesize that TTV7 may be associated with a higher total viral load in KD.
    MeSH term(s) Humans ; Immunoglobulins, Intravenous/therapeutic use ; Torque teno virus/genetics ; Mucocutaneous Lymph Node Syndrome/complications ; Aspartate Aminotransferases ; Viral Load ; Bilirubin ; Biomarkers ; C-Reactive Protein ; Retrospective Studies
    Chemical Substances Immunoglobulins, Intravenous ; Aspartate Aminotransferases (EC 2.6.1.1) ; Bilirubin (RFM9X3LJ49) ; Biomarkers ; C-Reactive Protein (9007-41-4)
    Language English
    Publishing date 2023-10-21
    Publishing country England
    Document type Journal Article
    ZDB-ID 2615211-3
    ISSN 2045-2322 ; 2045-2322
    ISSN (online) 2045-2322
    ISSN 2045-2322
    DOI 10.1038/s41598-023-45327-5
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article ; Online: Inhibiting immunoregulatory amidase NAAA blocks ZIKV maturation in Human Neural Stem Cells.

    Lai, Michele / La Rocca, Veronica / Iacono, Elena / Filipponi, Carolina / De Carli, Alessandro / Favaro, Domenico / Fonnesu, Rossella / Filippini, Fabio / Spezia, Pietro Giorgio / Amato, Rachele / Catelli, Elisa / Matteo, Baggiani / Lottini, Giulia / Onorati, Marco / Clementi, Nicola / Freer, Giulia / Piomelli, Daniele / Pistello, Mauro

    Antiviral research

    2023  Volume 216, Page(s) 105664

    Abstract: Recent evidence suggests that lipids play a crucial role in viral infections beyond their traditional functions of supplying envelope and energy, and creating protected niches for viral replication. In the case of Zika virus (ZIKV), it alters host lipids ...

    Abstract Recent evidence suggests that lipids play a crucial role in viral infections beyond their traditional functions of supplying envelope and energy, and creating protected niches for viral replication. In the case of Zika virus (ZIKV), it alters host lipids by enhancing lipogenesis and suppressing β-oxidation to generate viral factories at the endoplasmic reticulum (ER) interface. This discovery prompted us to hypothesize that interference with lipogenesis could serve as a dual antiviral and anti-inflammatory strategy to combat the replication of positive sense single-stranded RNA (ssRNA+) viruses. To test this hypothesis, we examined the impact of inhibiting N-Acylethanolamine acid amidase (NAAA) on ZIKV-infected human Neural Stem Cells. NAAA is responsible for the hydrolysis of palmitoylethanolamide (PEA) in lysosomes and endolysosomes. Inhibition of NAAA results in PEA accumulation, which activates peroxisome proliferator-activated receptor-α (PPAR-α), directing β-oxidation and preventing inflammation. Our findings indicate that inhibiting NAAA through gene-editing or drugs moderately reduces ZIKV replication by approximately one log
    MeSH term(s) Humans ; Amidohydrolases/antagonists & inhibitors ; Amidohydrolases/metabolism ; Anti-Inflammatory Agents/pharmacology ; Inflammation/drug therapy ; Zika Virus ; Zika Virus Infection/drug therapy
    Chemical Substances Amidohydrolases (EC 3.5.-) ; Anti-Inflammatory Agents ; NAAA protein, human (EC 3.5.1.-)
    Language English
    Publishing date 2023-07-04
    Publishing country Netherlands
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 306628-9
    ISSN 1872-9096 ; 0166-3542
    ISSN (online) 1872-9096
    ISSN 0166-3542
    DOI 10.1016/j.antiviral.2023.105664
    Database MEDical Literature Analysis and Retrieval System OnLINE

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