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  1. Article ; Online: Generation of post-surgical minimal residual disease models to investigate metastasis in soft tissue sarcoma patient-derived orthotopic xenografts.

    Fischer, Suzanne / Creytens, David / Gijsels, Stefanie / Descamps, Benedicte / Lapeire, Lore / Hendrix, An / Sys, Gwen / De Wever, Olivier

    STAR protocols

    2024  Volume 5, Issue 1, Page(s) 102863

    Abstract: Despite optimal multimodal treatment including surgical resection, 50%-80% of high-grade soft tissue sarcoma (STS) patients metastasize. Here, we present a protocol for the generation and use of post-surgical minimal residual disease models to ... ...

    Abstract Despite optimal multimodal treatment including surgical resection, 50%-80% of high-grade soft tissue sarcoma (STS) patients metastasize. Here, we present a protocol for the generation and use of post-surgical minimal residual disease models to investigate metastatic relapse in STS patient-derived xenografts. We describe steps for orthotopic engraftment of high-grade STS patient-derived tumor tissue. We then detail procedures for primary tumor resection with broad, negative resection margins and follow-up until metastases using MRI. For complete details on the use and execution of this protocol, please refer to Fischer et al. (2023).
    MeSH term(s) Humans ; Neoplasm, Residual ; Heterografts ; Sarcoma/diagnostic imaging ; Sarcoma/surgery ; Sarcoma/pathology ; Soft Tissue Neoplasms/diagnostic imaging ; Soft Tissue Neoplasms/surgery ; Soft Tissue Neoplasms/pathology ; Magnetic Resonance Imaging
    Language English
    Publishing date 2024-02-28
    Publishing country United States
    Document type Journal Article
    ISSN 2666-1667
    ISSN (online) 2666-1667
    DOI 10.1016/j.xpro.2024.102863
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: Identification and validation of extracellular vesicle reference genes for the normalization of RT-qPCR data.

    Pinheiro, Cláudio / Guilbert, Niké / Lippens, Lien / Roux, Quentin / Boiy, Robin / Fischer, Suzanne / Van Dorpe, Sofie / De Craene, Bram / Berx, Geert / Boterberg, Tom / Sys, Gwen / Denys, Hannelore / Miinalainen, Ilkka / Mestdagh, Pieter / Vandesompele, Jo / De Wever, Olivier / Hendrix, An

    Journal of extracellular vesicles

    2024  Volume 13, Issue 4, Page(s) e12421

    Abstract: Extracellular vesicles (EVs) contain a plethora of biomolecules, including nucleic acids, with diverse diagnostic and therapeutic application potential. Although reverse transcription-quantitative PCR (RT-qPCR) is the most widely applied laboratory ... ...

    Abstract Extracellular vesicles (EVs) contain a plethora of biomolecules, including nucleic acids, with diverse diagnostic and therapeutic application potential. Although reverse transcription-quantitative PCR (RT-qPCR) is the most widely applied laboratory technique to evaluate gene expression, its applicability in EV research is challenged by the lack of universal and stably present reference genes (RGs). In this study, we identify, validate and establish SNRPG, OST4, TOMM7 and NOP10 as RGs for the normalization of EV-associated genes by RT-qPCR. We show the stable presence of SNRPG, OST4, TOMM7 and NOP10 in multiple cell lines and their secreted EVs (n = 12) under different (patho)physiological conditions as well as in human-derived biofluids (n = 3). Enzymatic treatments confirm the presence of SNRPG, OST4, TOMM7 and NOP10 inside EVs. In addition, the four EV-associated RGs are stably detected in a size-range of EV subpopulations. RefFinder analysis reveals that SNRPG, OST4, TOMM7 and NOP10 are more stable compared to RGs established specifically for cultured cells or tissues such as HMBS, YWHAZ, SDHA and GAPDH. In summary, we present four universal and stably present EV-associated RGs to enable normalization and thus steer the implementation of RT-qPCR for the analysis of EV-associated RNA cargo for research or clinical applications.
    MeSH term(s) Humans ; Reverse Transcription ; Extracellular Vesicles/genetics ; Extracellular Vesicles/metabolism ; RNA/metabolism ; Cell Line ; Cells, Cultured ; snRNP Core Proteins/metabolism
    Chemical Substances RNA (63231-63-0) ; SNRPG protein, human ; snRNP Core Proteins
    Language English
    Publishing date 2024-03-28
    Publishing country United States
    Document type Journal Article
    ZDB-ID 2683797-3
    ISSN 2001-3078 ; 2001-3078
    ISSN (online) 2001-3078
    ISSN 2001-3078
    DOI 10.1002/jev2.12421
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article ; Online: Post-operative minimal residual disease models to study metastatic relapse in soft-tissue sarcoma patient-derived xenografts.

    Fischer, Suzanne / Creytens, David / De Geyter, Sofie / De Vlieghere, Elly / Pattyn, Piet / Bekaert, Sarah-Lee / Durinck, Kaat / Van Roy, Nadine / Hendrix, An / Lapeire, Lore / Sys, Gwen / De Wever, Olivier

    Clinical and translational medicine

    2023  Volume 13, Issue 6, Page(s) e1290

    MeSH term(s) Animals ; Humans ; Neoplasm, Residual ; Heterografts ; Neoplasm Recurrence, Local ; Sarcoma/pathology ; Sarcoma/surgery ; Soft Tissue Neoplasms/pathology ; Soft Tissue Neoplasms/surgery ; Disease Models, Animal
    Language English
    Publishing date 2023-06-07
    Publishing country United States
    Document type Letter
    ZDB-ID 2697013-2
    ISSN 2001-1326 ; 2001-1326
    ISSN (online) 2001-1326
    ISSN 2001-1326
    DOI 10.1002/ctm2.1290
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article ; Online: A host-microbiota interactome reveals extensive transkingdom connectivity.

    Sonnert, Nicole D / Rosen, Connor E / Ghazi, Andrew R / Franzosa, Eric A / Duncan-Lowey, Brianna / González-Hernández, Jaime A / Huck, John D / Yang, Yi / Dai, Yile / Rice, Tyler A / Nguyen, Mytien T / Song, Deguang / Cao, Yiyun / Martin, Anjelica L / Bielecka, Agata A / Fischer, Suzanne / Guan, Changhui / Oh, Julia / Huttenhower, Curtis /
    Ring, Aaron M / Palm, Noah W

    Nature

    2024  Volume 628, Issue 8006, Page(s) 171–179

    Abstract: The myriad microorganisms that live in close association with humans have diverse effects on physiology, yet the molecular bases for these impacts remain mostly ... ...

    Abstract The myriad microorganisms that live in close association with humans have diverse effects on physiology, yet the molecular bases for these impacts remain mostly unknown
    MeSH term(s) Animals ; Female ; Humans ; Mice ; Bacteria/classification ; Bacteria/immunology ; Bacteria/metabolism ; Bacteria/pathogenicity ; Host Microbial Interactions/immunology ; Host Microbial Interactions/physiology ; Host Tropism ; Microbiota/immunology ; Microbiota/physiology ; Organ Specificity ; Phylogeny ; Protein Binding ; Proteome/immunology ; Proteome/metabolism ; Reproducibility of Results ; Symbiosis
    Chemical Substances Proteome
    Language English
    Publishing date 2024-03-20
    Publishing country England
    Document type Journal Article
    ZDB-ID 120714-3
    ISSN 1476-4687 ; 0028-0836
    ISSN (online) 1476-4687
    ISSN 0028-0836
    DOI 10.1038/s41586-024-07162-0
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 26: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (1.OG)
    ab Jg. 2022: Lesesaal (EG)
    Zs.MG 9: Show issues
    Zs.MO 244: Show issues

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  5. Article ; Online: Compartmentalized ocular lymphatic system mediates eye-brain immunity.

    Yin, Xiangyun / Zhang, Sophia / Lee, Ju Hyun / Dong, Huiping / Mourgkos, George / Terwilliger, Gordon / Kraus, Aurora / Geraldo, Luiz Henrique / Poulet, Mathilde / Fischer, Suzanne / Zhou, Ting / Mohammed, Farrah Shalima / Zhou, Jiangbing / Wang, Yongfu / Malloy, Seth / Rohner, Nicolas / Sharma, Lokesh / Salinas, Irene / Eichmann, Anne /
    Thomas, Jean-Leon / Saltzman, W Mark / Huttner, Anita / Zeiss, Caroline / Ring, Aaron / Iwasaki, Akiko / Song, Eric

    Nature

    2024  Volume 628, Issue 8006, Page(s) 204–211

    Abstract: The eye, an anatomical extension of the central nervous system (CNS), exhibits many molecular and cellular parallels to the brain. Emerging research demonstrates that changes in the brain are often reflected in the eye, particularly in the ... ...

    Abstract The eye, an anatomical extension of the central nervous system (CNS), exhibits many molecular and cellular parallels to the brain. Emerging research demonstrates that changes in the brain are often reflected in the eye, particularly in the retina
    MeSH term(s) Animals ; Female ; Humans ; Male ; Mice ; Rabbits ; Bacteria/immunology ; Brain/anatomy & histology ; Brain/immunology ; Dependovirus/immunology ; Eye/anatomy & histology ; Eye/immunology ; Glioblastoma/immunology ; Herpesvirus 2, Human/immunology ; Intravitreal Injections ; Lymphatic System/anatomy & histology ; Lymphatic System/immunology ; Lymphatic Vessels/anatomy & histology ; Lymphatic Vessels/immunology ; Macaca mulatta ; Meninges/immunology ; Optic Nerve/immunology ; Swine ; Zebrafish ; Vascular Endothelial Growth Factor C/immunology ; Vascular Endothelial Growth Factor C/metabolism ; Vascular Endothelial Growth Factor C/pharmacology
    Chemical Substances Vascular Endothelial Growth Factor C
    Language English
    Publishing date 2024-02-28
    Publishing country England
    Document type Journal Article
    ZDB-ID 120714-3
    ISSN 1476-4687 ; 0028-0836
    ISSN (online) 1476-4687
    ISSN 0028-0836
    DOI 10.1038/s41586-024-07130-8
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 26: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (1.OG)
    ab Jg. 2022: Lesesaal (EG)
    Zs.MG 9: Show issues
    Zs.MO 244: Show issues

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  6. Article: Serum Calcification Propensity T50 Associates with Disease Severity in Patients with Pseudoxanthoma Elasticum.

    Nollet, Lukas / Van Gils, Matthias / Fischer, Suzanne / Campens, Laurence / Karthik, Swapna / Pasch, Andreas / De Zaeytijd, Julie / Leroy, Bart P / Devos, Daniel / De Backer, Tine / Coucke, Paul J / Vanakker, Olivier M

    Journal of clinical medicine

    2022  Volume 11, Issue 13

    Abstract: Pseudoxanthoma elasticum (PXE) is a currently intractable genetic disorder characterized by progressive ectopic calcification in the skin, eyes and arteries. Therapeutic trials in PXE are severely hampered by the lack of reliable biomarkers. Serum ... ...

    Abstract Pseudoxanthoma elasticum (PXE) is a currently intractable genetic disorder characterized by progressive ectopic calcification in the skin, eyes and arteries. Therapeutic trials in PXE are severely hampered by the lack of reliable biomarkers. Serum calcification propensity T50 is a blood test measuring the functional anticalcifying buffer capacity of serum. Here, we evaluated T50 in PXE patients aiming to investigate its determinants and suitability as a potential biomarker for disease severity. Fifty-seven PXE patients were included in this cross-sectional study, and demographic, clinical, imaging and biochemical data were collected from medical health records. PXE severity was assessed using Phenodex scores. T50 was measured using a validated, nephelometry-based assay. Multivariate models were then created to investigate T50 determinants and associations with disease severity. In short, the mean age of patients was 45.2 years, 68.4% was female and mean serum T50 was 347 min. Multivariate regression analysis identified serum fetuin-A (p < 0.001), phosphorus (p = 0.007) and magnesium levels (p = 0.034) as significant determinants of T50, while no correlations were identified with serum calcium, eGFR, plasma PPi levels or the ABCC6 genotype. After correction for covariates, T50 was found to be an independent determinant of ocular (p = 0.013), vascular (p = 0.013) and overall disease severity (p = 0.016) in PXE. To conclude, shorter serum T50—indicative of a higher calcification propensity—was associated with a more severe phenotype in PXE patients. This study indicates, for the first time, that serum T50 might be a clinically relevant biomarker in PXE and may thus be of importance to future therapeutic trials.
    Language English
    Publishing date 2022-06-28
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2662592-1
    ISSN 2077-0383
    ISSN 2077-0383
    DOI 10.3390/jcm11133727
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  7. Book ; Thesis: Diseases of men

    Fischer, Suzanne Michelle

    sexual health and medical expertise in advertising medical institutes, 1900-1930

    2009  

    Author's details Suzanne Michelle Fischer
    MeSH term(s) Marketing of Health Services/history ; Advertising as Topic/history ; Men's Health/history ; Sexually Transmitted Diseases/history ; History, 20th Century
    Keywords United States
    Language English
    Size iv, 250 p.
    Document type Book ; Thesis
    Thesis / German Habilitation thesis Thesis (Ph.D.)--University of Minnesota, 2009
    Database Catalogue of the US National Library of Medicine (NLM)

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  8. Book: Techniken und Materialien der Kunst

    Fischer, Suzanne / Fuga, Antonella

    (Bildlexikon der Kunst ; 10)

    2005  

    Title translation Tecniche e materiali delle arti <dt.>
    Author's details Antonella Fuga. [Aus dem Ital.: Suzanne Fischer ...]
    Series title Bildlexikon der Kunst ; 10
    Keywords Künstlerische Technik ; Künstlerisches Material
    Language German
    Size 383 S., überw. Ill., 20 cm
    Publisher Parthas
    Publishing place Berlin
    Document type Book
    Note Literaturverz. S. 382 - 383
    ISBN 3936324530 ; 3936324306 ; 9783936324532 ; 9783936324303
    Database Former special subject collection: coastal and deep sea fishing

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  9. Book: Techniken und Materialien der Kunst

    Fischer, Suzanne / Fuga, Antonella

    (Bildlexikon der Kunst ; 10)

    2005  

    Title translation Tecniche e materiali delle arti <dt.>
    Author's details Antonella Fuga. [Aus dem Ital.: Suzanne Fischer ...]
    Series title Bildlexikon der Kunst ; 10
    Keywords Künstlerische Technik ; Künstlerisches Material
    Language German
    Size 383 S., überw. Ill., 20 cm
    Publisher Parthas
    Publishing place Berlin
    Document type Book
    Note Literaturverz. S. 382 - 383
    ISBN 3936324530 ; 3936324306 ; 9783936324532 ; 9783936324303
    Database Library catalogue of the German National Library of Science and Technology (TIB), Hannover

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  10. Book: Engel, Dämonen und phantastische Wesen

    Fischer, Suzanne / Giorgi, Rosa

    (Bildlexikon der Kunst ; 6)

    2004  

    Title translation Angeli e demoni <dt.>
    Author's details Rosa Giorgi. [Aus dem Ital.: Suzanne Fischer ...]
    Series title Bildlexikon der Kunst ; 6
    Keywords Engel ; Dämon ; Kunst
    Language German
    Size 380 S., überw. Ill., 20 cm
    Publisher Parthas
    Publishing place Berlin
    Document type Book
    ISBN 3936324042 ; 9783936324044
    Database Former special subject collection: coastal and deep sea fishing

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