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  1. Book ; Online ; Thesis: Biochemical and functional characterization of DHX30, an RNA helicase linked to neurodevelopmental disorder

    Huber, Hannes [Verfasser] / Fischer, Utz [Gutachter]

    2023  

    Author's details Hannes Huber ; Gutachter: Utz Fischer
    Keywords Biowissenschaften, Biologie ; Life Science, Biology
    Subject code sg570
    Language English
    Publisher Universität Würzburg
    Publishing place Würzburg
    Document type Book ; Online ; Thesis
    Database Digital theses on the web

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  2. Book ; Online ; Thesis: The role of alternative intronic polyadenylation on microRNA biogenesis in melanoma

    Blahetek, Gina [Verfasser] / Vorlova, Sandra [Gutachter] / Fischer, Utz [Gutachter]

    2024  

    Author's details Gina Blahetek ; Gutachter: Sandra Vorlova, Utz Fischer
    Keywords Biowissenschaften, Biologie ; Life Science, Biology
    Subject code sg570
    Language English
    Publisher Universität Würzburg
    Publishing place Würzburg
    Document type Book ; Online ; Thesis
    Database Digital theses on the web

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  3. Article ; Online: lncRNA Malat1 and miR-26 cooperate in the regulation of neuronal progenitor cell proliferation and differentiation.

    Was, Nina / Sauer, Mark / Fischer, Utz / Becker, Matthias

    RNA (New York, N.Y.)

    2022  

    Abstract: Neurogenesis is a finely tuned process, which depends on the balanced execution of expression programs that regulate cellular differentiation and proliferation. Different molecular players ranging from transcription factors to chromatin modulators ... ...

    Abstract Neurogenesis is a finely tuned process, which depends on the balanced execution of expression programs that regulate cellular differentiation and proliferation. Different molecular players ranging from transcription factors to chromatin modulators control these programs. Adding to the complexity, also non-coding (nc)RNAs take part in this process. Here we analyzed the function of the long non-coding (lnc)RNA Malat1 during neural embryonic stem cell (ESC) differentiation. We find that deletion of Malat1 leads to inhibition of proliferation of neural progenitor cells (NPCs). Interestingly, this co-insides with an increase in the expression of miR-26 family members miR-26a and miR-26b in differentiating ESCs. Inactivation of miR-26a/b rescues the proliferative phenotype of Malat1 knockout (KO) cells and leads to accelerated neuronal differentiation of compound Malat1KO/mir-26KO ESCs. Together our work identifies a so far unknown interaction between Malat1 and miR-26 in the regulation of NPC proliferation and neuronal differentiation.
    Language English
    Publishing date 2022-10-27
    Publishing country United States
    Document type Journal Article
    ZDB-ID 1241540-6
    ISSN 1469-9001 ; 1355-8382
    ISSN (online) 1469-9001
    ISSN 1355-8382
    DOI 10.1261/rna.079436.122
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 4777: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (2.OG)
    ab Jg. 2022: Lesesaal (EG)

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  4. Article: Cytoplasmic gene expression: lessons from poxviruses.

    Grimm, Clemens / Bartuli, Julia / Fischer, Utz

    Trends in biochemical sciences

    2022  Volume 47, Issue 10, Page(s) 892–902

    Abstract: In eukaryotic cells, the process of gene expression is confined to the nucleus and enabled by multisubunit RNA polymerases (RNAPs). Many viruses make use of the host cellular gene expression apparatus during infection, and hence transfer their genome at ... ...

    Abstract In eukaryotic cells, the process of gene expression is confined to the nucleus and enabled by multisubunit RNA polymerases (RNAPs). Many viruses make use of the host cellular gene expression apparatus during infection, and hence transfer their genome at least transiently to the host nucleus. However, poxviruses have evolved a different strategy to propagate. Their double-stranded DNA genome is transcribed in the host cytoplasm by a virus-encoded RNAP (vRNAP), which is evolutionarily related to eukaryotic RNA polymerase II. In this Review, we highlight recent high-resolution structures of the poxviral transcription apparatus in different phases of action. These structures, along with biochemical data, now allow the definition of a comprehensive model of poxviral gene expression and its regulation.
    MeSH term(s) Cell Nucleus/genetics ; Cytoplasm/genetics ; DNA-Directed RNA Polymerases/chemistry ; Gene Expression ; Poxviridae/genetics ; RNA Polymerase II/genetics ; Transcription, Genetic
    Chemical Substances RNA Polymerase II (EC 2.7.7.-) ; DNA-Directed RNA Polymerases (EC 2.7.7.6)
    Language English
    Publishing date 2022-05-15
    Publishing country England
    Document type Journal Article ; Review
    ZDB-ID 194216-5
    ISSN 1362-4326 ; 0968-0004 ; 0376-5067
    ISSN (online) 1362-4326
    ISSN 0968-0004 ; 0376-5067
    DOI 10.1016/j.tibs.2022.04.010
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 1207: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (1.OG)
    ab Jg. 2022: Lesesaal (EG)

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  5. Book ; Online ; Thesis: Identifizierung und Untersuchung TOP-mRNA - bindender Faktoren

    Amelingmeier, Florian [Verfasser] / Fischer, Utz [Gutachter]

    2022  

    Author's details Florian Amelingmeier ; Gutachter: Utz Fischer
    Keywords Biowissenschaften, Biologie ; Life Science, Biology
    Subject code sg570
    Language German
    Publisher Universität Würzburg
    Publishing place Würzburg
    Document type Book ; Online ; Thesis
    Database Digital theses on the web

    Full text online

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    Inter-library loan at ZB MED

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  6. Book ; Online ; Thesis: Biochemical and structural characterisation of modules within the SMN complex

    Viswanathan, Aravindan [Verfasser] / Fischer, Utz [Gutachter]

    2022  

    Author's details Aravindan Viswanathan ; Gutachter: Utz Fischer
    Keywords Biowissenschaften, Biologie ; Life Science, Biology
    Subject code sg570
    Language English
    Publisher Universität Würzburg
    Publishing place Würzburg
    Document type Book ; Online ; Thesis
    Database Digital theses on the web

    Full text online

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    Inter-library loan at ZB MED

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  7. Article: Cytoplasmic gene expression: lessons from poxviruses

    Grimm, Clemens / Bartuli, Julia / Fischer, Utz

    Trends in biochemical sciences. 2022,

    2022  

    Abstract: In eukaryotic cells, the process of gene expression is confined to the nucleus and enabled by multisubunit RNA polymerases (RNAPs). Many viruses make use of the host cellular gene expression apparatus during infection, and hence transfer their genome at ... ...

    Abstract In eukaryotic cells, the process of gene expression is confined to the nucleus and enabled by multisubunit RNA polymerases (RNAPs). Many viruses make use of the host cellular gene expression apparatus during infection, and hence transfer their genome at least transiently to the host nucleus. However, poxviruses have evolved a different strategy to propagate. Their double-stranded DNA genome is transcribed in the host cytoplasm by a virus-encoded RNAP (vRNAP), which is evolutionarily related to eukaryotic RNA polymerase II. In this Review, we highlight recent high-resolution structures of the poxviral transcription apparatus in different phases of action. These structures, along with biochemical data, now allow the definition of a comprehensive model of poxviral gene expression and its regulation.
    Keywords DNA ; DNA-directed RNA polymerase ; Poxviridae ; RNA ; cytoplasm ; gene expression ; genome ; models
    Language English
    Publishing place Elsevier Ltd
    Document type Article
    Note Pre-press version
    ZDB-ID 194220-7
    ISSN 0968-0004 ; 0376-5067
    ISSN 0968-0004 ; 0376-5067
    DOI 10.1016/j.tibs.2022.04.010
    Database NAL-Catalogue (AGRICOLA)

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    In stock of ZB MED Bonn / Germany

    Z 78/716: Show issues

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  8. Article ; Online: Dirac Cones and Room Temperature Polariton Lasing Evidenced in an Organic Honeycomb Lattice.

    Betzold, Simon / Düreth, Johannes / Dusel, Marco / Emmerling, Monika / Bieganowska, Antonina / Ohmer, Jürgen / Fischer, Utz / Höfling, Sven / Klembt, Sebastian

    Advanced science (Weinheim, Baden-Wurttemberg, Germany)

    2024  , Page(s) e2400672

    Abstract: Artificial 1D and 2D lattices have emerged as a powerful platform for the emulation of lattice Hamiltonians, the fundamental study of collective many-body effects, and phenomena arising from non-trivial topology. Exciton-polaritons, bosonic part-light ... ...

    Abstract Artificial 1D and 2D lattices have emerged as a powerful platform for the emulation of lattice Hamiltonians, the fundamental study of collective many-body effects, and phenomena arising from non-trivial topology. Exciton-polaritons, bosonic part-light and part-matter quasiparticles, combine pronounced nonlinearities with the possibility of on-chip implementation. In this context, organic semiconductors embedded in microcavities have proven to be versatile candidates to study nonlinear many-body physics and bosonic condensation, and in contrast to most inorganic systems, they allow the use at ambient conditions since they host ultra-stable Frenkel excitons. A well-controlled, high-quality optical lattice is implemented that accommodates light-matter quasiparticles. The realized polariton graphene presents with excellent cavity quality factors, showing distinct signatures of Dirac cone and flatband dispersions as well as polariton lasing at room temperature. This is realized by filling coupled dielectric microcavities with the fluorescent protein mCherry. The emergence of a coherent polariton condensate at ambient conditions are demonstrated, taking advantage of coupling conditions as precise and controllable as in state-of-the-art inorganic semiconductor-based systems, without the limitations of e.g. lattice matching in epitaxial growth. This progress allows straightforward extension to more complex systems, such as the study of topological phenomena in 2D lattices including topological lasers and non-Hermitian optics.
    Language English
    Publishing date 2024-04-12
    Publishing country Germany
    Document type Journal Article
    ZDB-ID 2808093-2
    ISSN 2198-3844 ; 2198-3844
    ISSN (online) 2198-3844
    ISSN 2198-3844
    DOI 10.1002/advs.202400672
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  9. Article: Structure and function of the poxvirus transcription machinery.

    Fischer, Utz / Bartuli, Julia / Grimm, Clemens

    The Enzymes

    2021  Volume 50, Page(s) 1–20

    Abstract: Members of the Poxviridae family are large double-stranded DNA viruses that replicate exclusively in the cytoplasm of their hosts. This goes in hand with a high level of independence from the host cell, which supports transcription and replication events ...

    Abstract Members of the Poxviridae family are large double-stranded DNA viruses that replicate exclusively in the cytoplasm of their hosts. This goes in hand with a high level of independence from the host cell, which supports transcription and replication events only in the nucleus or in DNA-containing organelles. Consequently, virus specific, rather than cellular enzymes mediate most processes involving DNA replication and mRNA synthesis. Recent technological advances allowed a detailed functional and structural investigation of the transcription machinery of the prototypic poxvirus vaccinia. The DNA-dependent RNA polymerase (RNAP) at its core displays distinct similarities to eukaryotic RNAPs. Strong idiosyncrasies, however, are apparent for viral factors that are associated with the viral RNAP during mRNA production. We expect that future studies will unravel more key aspects of poxvirus gene expression, helping also the understanding of nuclear transcription mechanisms.
    MeSH term(s) DNA-Directed RNA Polymerases ; Poxviridae/genetics ; Transcription, Genetic ; Viral Proteins/genetics ; Virus Replication
    Chemical Substances Viral Proteins ; DNA-Directed RNA Polymerases (EC 2.7.7.6)
    Language English
    Publishing date 2021-07-24
    Publishing country United States
    Document type Journal Article
    ISSN 0423-2607
    ISSN 0423-2607
    DOI 10.1016/bs.enz.2021.06.005
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  10. Article ; Online: A generic protocol for the affinity-purification of native macromolecular complexes from poxvirus-infected cells.

    Bartuli, Julia / Lorenzi, Isotta / Backes, Simone / Grimm, Clemens / Fischer, Utz

    STAR protocols

    2022  Volume 3, Issue 1, Page(s) 101116

    Abstract: The functional and structural characterization of macromolecular complexes requires protocols for their native isolation. Here, we describe a protocol for this task based on the recombinant poxvirus Vaccinia expressing tagged proteins of interest in ... ...

    Abstract The functional and structural characterization of macromolecular complexes requires protocols for their native isolation. Here, we describe a protocol for this task based on the recombinant poxvirus Vaccinia expressing tagged proteins of interest in infected cells. Tagged proteins and their interactors can then be isolated via affinity chromatography. The procedure is illustrated for the Vaccinia virus encoded multi-subunit RNA polymerase. Our protocol also allows the expression and isolation of heterologous proteins and hence is suitable for a broader application. For complete details on the use and execution of this profile, please refer to Grimm et al. (2019).
    MeSH term(s) Chromatography, Affinity/methods ; DNA-Directed RNA Polymerases ; Indicators and Reagents ; Macromolecular Substances ; Proteins ; Vaccinia virus/genetics
    Chemical Substances Indicators and Reagents ; Macromolecular Substances ; Proteins ; DNA-Directed RNA Polymerases (EC 2.7.7.6)
    Language English
    Publishing date 2022-01-21
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ISSN 2666-1667
    ISSN (online) 2666-1667
    DOI 10.1016/j.xpro.2021.101116
    Database MEDical Literature Analysis and Retrieval System OnLINE

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