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Article ; Online: Optimization of a hybrid bacterial/Arabidopsis thaliana fatty acid synthase system II in Saccharomyces cerevisiae

Tatiana A. Pozdniakova / João P. Cruz / Paulo César Silva / Flávio Azevedo / Pier Parpot / Maria Rosario Domingues / Magnus Carlquist / Björn Johansson

Metabolic Engineering Communications, Vol 17, Iss , Pp e00224- (2023)

2023

Abstract: Fatty acids are produced by eukaryotes like baker's yeast Saccharomyces cerevisiae mainly using a large multifunctional type I fatty acid synthase (FASI) where seven catalytic steps and a carrier domain are shared between one or two protein subunits. ... ...

Abstract	Fatty acids are produced by eukaryotes like baker's yeast Saccharomyces cerevisiae mainly using a large multifunctional type I fatty acid synthase (FASI) where seven catalytic steps and a carrier domain are shared between one or two protein subunits. While this system may offer efficiency in catalysis, only a narrow range of fatty acids are produced. Prokaryotes, chloroplasts and mitochondria rely instead on a FAS type II (FASII) where each catalytic step is carried out by a monofunctional enzyme encoded by a separate gene. FASII is more flexible and capable of producing a wider range of fatty acid structures, such as the direct production of unsaturated fatty acids. An efficient FASII in the preferred industrial organism S. cerevisiae could provide a platform for developing sustainable production of specialized fatty acids. We functionally replaced either yeast FASI genes (FAS1 or FAS2) with a FASII consisting of nine genes from Escherichia coli (acpP, acpS and fab -A, -B, -D, -F, -G, -H, -Z) as well as three from Arabidopsis thaliana (MOD1, FATA1 and FATB). The genes were expressed from an autonomously replicating multicopy vector assembled using the Yeast Pathway Kit for in-vivo assembly in yeast. Two rounds of adaptation led to a strain with a maximum growth rate (μmax) of 0.19 h−1 without exogenous fatty acids, twice the growth rate previously reported for a comparable strain. Additional copies of the MOD1 or fabH genes resulted in cultures with higher final cell densities and three times higher lipid content compared to the control.
Keywords	Saccharomyces cerevisiae ; E. coli ; Fatty acid synthase ; FASI ; FASII ; Metabolic engineering ; Biotechnology ; TP248.13-248.65 ; Biology (General) ; QH301-705.5
Subject code	571
Language	English
Publishing date	2023-12-01T00:00:00Z
Publisher	Elsevier
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: A novel d-xylose isomerase from the gut of the wood feeding beetle Odontotaenius disjunctus efficiently expressed in Saccharomyces cerevisiae

Paulo César Silva / Javier A. Ceja-Navarro / Flávio Azevedo / Ulas Karaoz / Eoin L. Brodie / Björn Johansson

Scientific Reports, Vol 11, Iss 1, Pp 1-

2021 Volume 12

Abstract: Abstract Carbohydrate rich substrates such as lignocellulosic hydrolysates remain one of the primary sources of potentially renewable fuel and bulk chemicals. The pentose sugar d-xylose is often present in significant amounts along with hexoses. ... ...

Abstract	Abstract Carbohydrate rich substrates such as lignocellulosic hydrolysates remain one of the primary sources of potentially renewable fuel and bulk chemicals. The pentose sugar d-xylose is often present in significant amounts along with hexoses. Saccharomyces cerevisiae can acquire the ability to metabolize d-xylose through expression of heterologous d-xylose isomerase (XI). This enzyme is notoriously difficult to express in S. cerevisiae and only fourteen XIs have been reported to be active so far. We cloned a new d-xylose isomerase derived from microorganisms in the gut of the wood-feeding beetle Odontotaenius disjunctus. Although somewhat homologous to the XI from Piromyces sp. E2, the new gene was identified as bacterial in origin and the host as a Parabacteroides sp. Expression of the new XI in S. cerevisiae resulted in faster aerobic growth than the XI from Piromyces on d-xylose media. The d-xylose isomerization rate conferred by the new XI was also 72% higher, while absolute xylitol production was identical in both strains. Interestingly, increasing concentrations of xylitol (up to 8 g L−1) appeared not to inhibit d-xylose consumption. The newly described XI displayed 2.6 times higher specific activity, 37% lower KM for d-xylose, and exhibited higher activity over a broader temperature range, retaining 51% of maximal activity at 30 °C compared with only 29% activity for the Piromyces XI.
Keywords	Medicine ; R ; Science ; Q
Language	English
Publishing date	2021-02-01T00:00:00Z
Publisher	Nature Portfolio
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Towards a culture of open scholarship

Flávio Azevedo / Meng Liu / Charlotte R. Pennington / Madeleine Pownall / Thomas Rhys Evans / Sam Parsons / Mahmoud Medhat Elsherif / Leticia Micheli / Samuel J. Westwood / Framework for Open, Reproducible Research Training (FORRT)

BMC Research Notes, Vol 15, Iss 1, Pp 1-

the role of pedagogical communities

2022 Volume 5

Abstract: Abstract The UK House of Commons Science and Technology Committee has called for evidence on the roles that different stakeholders play in reproducibility and research integrity. Of central priority are proposals for improving research integrity and ... ...

Abstract	Abstract The UK House of Commons Science and Technology Committee has called for evidence on the roles that different stakeholders play in reproducibility and research integrity. Of central priority are proposals for improving research integrity and quality, as well as guidance and support for researchers. In response to this, we argue that there is one important component of research integrity that is often absent from discussion: the pedagogical consequences of how we teach, mentor, and supervise students through open scholarship. We justify the need to integrate open scholarship principles into research training within higher education and argue that pedagogical communities play a key role in fostering an inclusive culture of open scholarship. We illustrate these benefits by presenting the Framework for Open and Reproducible Research Training (FORRT), an international grassroots community whose goal is to provide support, resources, visibility, and advocacy for the adoption of principled, open teaching and mentoring practices, whilst generating conversations about the ethics and social impact of higher-education pedagogy. Representing a diverse group of early-career researchers and students across specialisms, we advocate for greater recognition of and support for pedagogical communities, and encourage all research stakeholders to engage with these communities to enable long-term, sustainable change.
Keywords	Open scholarship ; Open educational resources ; Open science ; Open research ; Pedagogy ; Reproducibility ; Medicine ; R ; Biology (General) ; QH301-705.5 ; Science (General) ; Q1-390
Subject code	020
Language	English
Publishing date	2022-02-01T00:00:00Z
Publisher	BMC
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article: Os rumos das pesquisas sobre a história econômica do Brasil

Saes, Flávio Azevedo Marques de

Leituras de economia política : revista dos estudiantes de pós-graduação do Instituto de Economia, Unicamp ; LEP Vol. 21 (2012/2013), p. 3-34

uma breve nota

2012 Volume 21, Page(s) 3–34

Author's details	Flávio Azevedo Marques de Saes
Language	Portuguese
Publishing place	Campinas, SP
Document type	Article
ZDB-ID	2393382-3 ; 2393384-7
ISSN	1415-6245
ISSN	1415-6245
Database	ECONomics Information System

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Article ; Online: Radiation caries

Eduardo Sérgio Donato Duarte Filho / Paula Fernanda Rodrigues de Melo Silva / Lúcio Flávio Azevedo Donato / Lúcio Frigo / Michel Nicolau Youssef / Stefânia Jeronimo Ferreira

Revista Cubana de Estomatología, Vol 56, Iss 1, Pp 86-

effects of radiotherapy on dental structure

2019 Volume 92

Abstract: Introduction: Patients undergoing radiotherapy as treatment for malignant head and neck tumors are prone to develop oral complications such as radiation caries, which evolves rapidly and is highly destructive. Such development is known to be caused by ... ...

Abstract	Introduction: Patients undergoing radiotherapy as treatment for malignant head and neck tumors are prone to develop oral complications such as radiation caries, which evolves rapidly and is highly destructive. Such development is known to be caused by post-radiotherapy alterations in salivary glands resulting in hyposalivation and changes in salivary components. However, it has been accepted that damage by direct radiation to the dental structure may hasten the progress of caries development, and studies have shown the morphological and physical changes occurring in human teeth after radiotherapy. Objective: Present a case of radiation caries and the direct effects of radiation on dental structure. Case presentation: A 60-year-old male patient complains of "dental fragility". The patient was diagnosed with squamous-cell carcinoma of the tongue 18 months ago and indicated chemotherapy (38 sessions), radiotherapy (38 sessions) and surgery. Physical examination revealed among other changes the presence of blackish cervical areas with quite fragile crowns in all remaining teeth, which are characteristic of radiation caries. Non-traumatic treatment was performed and the patient is now under observation. Conclusions: Salivary quantitative and qualitative changes are the main cause of radiation caries. Discussion about the direct effects of radiation on dental structure is still quite controversial in the literature on the topic. It is therefore suggested to conduct further studies in a more systematic, complete and standardized manner.
Keywords	câncer ; radioterapia ; cárie dental ; Dentistry ; RK1-715 ; Medicine (General) ; R5-920
Subject code	616
Language	English
Publishing date	2019-02-01T00:00:00Z
Publisher	Editorial Ciencias Médicas
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: A moeda, o crédito e o financiamento da produção

Flávio Azevedo Marques de Saes

Estudos Avançados, Vol 15, Iss 41, Pp 77-

2001 Volume 90

Abstract: O OBJETIVO do autor neste artigo foi mostrar as principais contribuições do pensamento econômico brasileiro da segunda metade do século XX para o estudo da moeda, do crédito e do financiamento da produção. Três temas gerais orientaram a exposição: ... ...

Abstract	O OBJETIVO do autor neste artigo foi mostrar as principais contribuições do pensamento econômico brasileiro da segunda metade do século XX para o estudo da moeda, do crédito e do financiamento da produção. Três temas gerais orientaram a exposição: história monetária e bancária; teoria monetária; crédito e financiamento. THE AIM of the author in this paper was to show the most important contributions of Brazilian economic thought in the second half of the 20th century to the study of money, credit and finance. Three main themes were reviewed: monetary and banking history; monetary theory; credit and finance.
Keywords	Social sciences (General) ; H1-99
Language	Spanish
Publishing date	2001-04-01T00:00:00Z
Publisher	Universidade de São Paulo
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Cell wall dynamics modulate acetic acid-induced apoptotic cell death of Saccharomyces cerevisiae

António Rego / Ana Marta Duarte / Flávio Azevedo / Maria João Sousa / Manuela Côrte-Real / Susana R. Chaves

Microbial Cell, Vol 1, Iss 9, Pp 303-

2014 Volume 314

Abstract: Acetic acid triggers apoptotic cell death in Saccharomyces cerevisiae, similar to mammalian apoptosis. To uncover novel regulators of this process, we analyzed whether impairing MAPK signaling affected acetic acid-induced apoptosis and found the mating- ... ...

Abstract	Acetic acid triggers apoptotic cell death in Saccharomyces cerevisiae, similar to mammalian apoptosis. To uncover novel regulators of this process, we analyzed whether impairing MAPK signaling affected acetic acid-induced apoptosis and found the mating-pheromone response and, especially, the cell wall integrity pathways were the major mediators, especially the latter, which we characterized further. Screening downstream effectors of this pathway, namely targets of the transcription factor Rlm1p, highlighted decreased cell wall remodeling as particularly important for acetic acid resistance. Modulation of cell surface dynamics therefore emerges as a powerful strategy to increase acetic acid resistance, with potential application in industrial fermentations using yeast, and in biomedicine to exploit the higher sensitivity of colorectal carcinoma cells to apoptosis induced by acetate produced by intestinal propionibacteria.
Keywords	yeast ; apoptosis ; acetic acid ; MAPK ; CWI ; Biology (General) ; QH301-705.5
Language	English
Publishing date	2014-08-01T00:00:00Z
Publisher	Shared Science Publishers OG
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: C2-phytoceramide perturbs lipid rafts and cell integrity in Saccharomyces cerevisiae in a sterol-dependent manner.

Andreia Pacheco / Flávio Azevedo / António Rego / Júlia Santos / Susana R Chaves / Manuela Côrte-Real / Maria João Sousa

PLoS ONE, Vol 8, Iss 9, p e

2013 Volume 74240

Abstract: Specific ceramides are key regulators of cell fate, and extensive studies aimed to develop therapies based on ceramide-induced cell death. However, the mechanisms regulating ceramide cytotoxicity are not yet fully elucidated. Since ceramides also ... ...

Abstract	Specific ceramides are key regulators of cell fate, and extensive studies aimed to develop therapies based on ceramide-induced cell death. However, the mechanisms regulating ceramide cytotoxicity are not yet fully elucidated. Since ceramides also regulate growth and stress responses in yeast, we studied how different exogenous ceramides affect yeast cells. C2-phytoceramide, a soluble form of phytoceramides, the yeast counterparts of mammalian ceramides, greatly reduced clonogenic survival, particularly in the G2/M phase, but did not induce autophagy nor increase apoptotic markers. Rather, the loss of clonogenic survival was associated with PI positive staining, disorganization of lipid rafts and cell wall weakening. Sensitivity to C2-phytoceramide was exacerbated in mutants lacking Hog1p, the MAP kinase homolog of human p38 kinase. Decreasing sterol membrane content reduced sensitivity to C2-phytoceramide, suggesting sterols are the targets of this compound. This study identified a new function of C2-phytoceramide through disorganization of lipid rafts and induction of a necrotic cell death under hypo-osmotic conditions. Since lipid rafts are important in mammalian cell signaling and adhesion, our findings further support pursuing the exploitation of yeast to understand the basis of synthetic ceramides' cytotoxicity to provide novel strategies for therapeutic intervention in cancer and other diseases.
Keywords	Medicine ; R ; Science ; Q
Subject code	571
Language	English
Publishing date	2013-01-01T00:00:00Z
Publisher	Public Library of Science (PLoS)
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article: The protective role of yeast Cathepsin D in acetic acid-induced apoptosis depends on ANT (Aac2p) but not on the voltage-dependent channel (Por1p)

Pereira, Helena / Flávio Azevedo / António Rego / Maria João Sousa / Susana R. Chaves / Manuela Côrte-Real

Federation of European Biochemical Societies FEBS letters. 2013 Jan. 16, v. 587, no. 2

2013

Abstract: We have previously shown that the yeast Cathepsin D (CatD) Pep4p translocates from the vacuole to the cytosol during acetic acid-induced apoptosis and is required for efficient mitochondrial degradation, though its specific role in this process is still ... ...

Abstract	We have previously shown that the yeast Cathepsin D (CatD) Pep4p translocates from the vacuole to the cytosol during acetic acid-induced apoptosis and is required for efficient mitochondrial degradation, though its specific role in this process is still elusive. Here, we show that the protective role of Pep4p in acetic acid-induced apoptosis depends on its catalytic activity and is independent of the yeast voltage-dependent anion channel Por1p (which has no role on mitochondrial degradation) but dependent on AAC proteins, the yeast adenine nucleotide translocator. Our results demonstrate a differential interplay between yeast vacuolar CatD and mitochondrial proteins involved in apoptosis regulation.
Keywords	adenine ; apoptosis ; catalytic activity ; cathepsin D ; cytosol ; protective effect ; proteins ; vacuoles ; yeasts
Language	English
Dates of publication	2013-0116
Size	p. 200-205.
Publishing place	Elsevier B.V.
Document type	Article
ZDB-ID	212746-5
ISSN	1873-3468 ; 0014-5793
ISSN (online)	1873-3468
ISSN	0014-5793
DOI	10.1016/j.febslet.2012.11.025
Database	NAL-Catalogue (AGRICOLA)

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Article ; Online: The Psychological Science Accelerator’s COVID-19 rapid-response dataset

Erin M. Buchanan / Savannah C. Lewis / Bastien Paris / Patrick S. Forscher / Jeffrey M. Pavlacic / Julie E. Beshears / Shira Meir Drexler / Amélie Gourdon-Kanhukamwe / Peter R Mallik / Miguel Alejandro A. Silan / Jeremy K. Miller / Hans IJzerman / Hannah Moshontz / Jennifer L. Beaudry / Jordan W. Suchow / Christopher R. Chartier / Nicholas A. Coles / MohammadHasan Sharifian / Anna Louise Todsen /

Carmel A. Levitan / Flávio Azevedo / Nicole Legate / Blake Heller / Alexander J. Rothman / Charles A. Dorison / Brian P. Gill / Ke Wang / Vaughan W. Rees / Nancy Gibbs / Amit Goldenberg / Thuy-vy Thi Nguyen / James J. Gross / Gwenaêl Kaminski / Claudia C. von Bastian / Mariola Paruzel-Czachura / Farnaz Mosannenzadeh / Soufian Azouaghe / Alexandre Bran / Susana Ruiz-Fernandez / Anabela Caetano Santos / Niv Reggev / Janis H. Zickfeld / Handan Akkas / Myrto Pantazi / Ivan Ropovik / Max Korbmacher / Patrícia Arriaga / Biljana Gjoneska / Lara Warmelink / Sara G. Alves

Scientific Data, Vol 10, Iss 1, Pp 1-

2023 Volume 15

Abstract: Measurement(s) COVID-19 Protocols, Restrictions • Health Behaviors • Personality and Behavioral Change, CTCAE • Emotion • Message Framing • Self-Determination Technology Type(s) Survey • Experiment Design Type Factor Type(s) COVID-19 Protocols, ... ...

Abstract	Measurement(s) COVID-19 Protocols, Restrictions • Health Behaviors • Personality and Behavioral Change, CTCAE • Emotion • Message Framing • Self-Determination Technology Type(s) Survey • Experiment Design Type Factor Type(s) COVID-19 Protocols, Restrictions • Health Behaviors • Loss-Gain Framing • Cognitive Reappraisal • Self-Determination Messaging Sample Characteristic - Organism Homo Sample Characteristic - Environment Daily Life Sample Characteristic - Location North America • South America • Africa • Australia • Europe • United Kingdom • Asia
Keywords	Science ; Q
Language	English
Publishing date	2023-02-01T00:00:00Z
Publisher	Nature Portfolio
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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