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Book ; Online ; Thesis: Implication of Bcl-2 family members in apoptosis signalling pathways triggered by growth factor deprivation

Fleischer, Aarne

2003

Author's details	vorgelegt von Aarne Fleischer
Language	English
Size	Online-Ressource
Document type	Book ; Online ; Thesis
Thesis / German Habilitation thesis	Techn. Hochsch., Diss--Aachen, 2003
Database	Former special subject collection: coastal and deep sea fishing

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Article ; Online: Hsa-Mir-320c, Hsa-Mir-200c-3p, and Hsa-Mir-449c-5p as Potential Specific miRNA Biomarkers of COPD: A Pilot Study.

Cerón-Pisa, Noemi / Iglesias, Amanda / Shafiek, Hanaa / Martín-Medina, Aina / Esteva-Socias, Margalida / Muncunill, Josep / Fleischer, Aarne / Verdú, Javier / Cosío, Borja G / Sauleda, Jaume

Pathophysiology : the official journal of the International Society for Pathophysiology

2022 Volume 29, Issue 2, Page(s) 143–156

Abstract: Chronic obstructive pulmonary disease (COPD) is a chronic inflammatory disease commonly induced by cigarette smoke. The expression of miRNAs can be altered in patients with COPD and could be used as a biomarker. We aimed to identify a panel of miRNAs in ... ...

Abstract	Chronic obstructive pulmonary disease (COPD) is a chronic inflammatory disease commonly induced by cigarette smoke. The expression of miRNAs can be altered in patients with COPD and could be used as a biomarker. We aimed to identify a panel of miRNAs in bronchoalveolar lavage (BAL) to differentiate COPD patients from smokers and non-smokers with normal lung function. Accordingly, forty-five subjects classified as COPD, smokers, and non-smokers (
Language	English
Publishing date	2022-03-29
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	1212740-1
ISSN	1873-149X ; 0928-4680
ISSN (online)	1873-149X
ISSN	0928-4680
DOI	10.3390/pathophysiology29020013
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: Generation of a human iPSC line (IMEDEAi008-A) derived from natural homozygous CCR5-Δ32 PBMCs enriched in the pro-erythroblast population.

Vallejo-Diez, Sara / Martín-Fernández, José María / Sánchez-Gilabert, Almudena / Fleischer, Aarne / Gayá, Antoni / Castresana, Mónica / Bachiller, Daniel

Stem cell research

2020 Volume 47, Page(s) 101918

Abstract: A 32 base pair deletion in the C-C chemokine receptor type gene (CCR5-Δ32), the main Human Immunodeficiency Virus (HIV) co-receptor results in a non-functional protein. Individuals homozygous for the CCR5-Δ32 mutation are resistant to HIV infection. Here ...

Abstract	A 32 base pair deletion in the C-C chemokine receptor type gene (CCR5-Δ32), the main Human Immunodeficiency Virus (HIV) co-receptor results in a non-functional protein. Individuals homozygous for the CCR5-Δ32 mutation are resistant to HIV infection. Here we report the generation, from pro-erythroblast enriched Peripheral Blood Mononuclear Cells (PBMCs) from a naturally occurring CCR5-Δ32/Δ32 individual, of the fully characterized iPSC line IMEDEAi008-A. The new line has normal karyotype, carry the Δ32 mutation in homozygosity, is free of plasmid integrations, express high levels of pluripotency markers and can differentiate into all three germ layers.
Language	English
Publishing date	2020-07-17
Publishing country	England
Document type	Journal Article
ZDB-ID	2393143-7
ISSN	1876-7753 ; 1873-5061
ISSN (online)	1876-7753
ISSN	1873-5061
DOI	10.1016/j.scr.2020.101918
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: Generation of one iPSC line (IMEDEAi007-A) by Sendai Virus transduction of PBMCs from a Psoriasis donor.

Vallejo-Diez, Sara / Fleischer, Aarne / María Martín-Fernández, José / Sánchez-Gilabert, Almudena / Gómez-Martínez, Carmelo / Castresana, Mónica / Bachiller, Daniel

Stem cell research

2020 Volume 47, Page(s) 101917

Abstract: Psoriasis is a chronic inflammatory skin disease that speeds up the life cycle of skin cells, forming scales and red patches that are itchy and sometimes painful. It is a complex disease of autoimmune origin and genetic predisposition with more than 10 ... ...

Abstract	Psoriasis is a chronic inflammatory skin disease that speeds up the life cycle of skin cells, forming scales and red patches that are itchy and sometimes painful. It is a complex disease of autoimmune origin and genetic predisposition with more than 10 different loci associated. Here we described the production of an iPSC line generated by Sendai Virus (Klf4, Oct3/4, Sox2 and c-Myc) reprogramming of Peripheral Blood Mononuclear Cells (PBMCs) from a Psoriasis patient. The iPSC line generated has normal 46XY karyotype, is free of SeV genome and transgenes insertions, express high levels of pluripotency markers and can differentiate into all three germ layers.
Language	English
Publishing date	2020-07-17
Publishing country	England
Document type	Journal Article
ZDB-ID	2393143-7
ISSN	1876-7753 ; 1873-5061
ISSN (online)	1876-7753
ISSN	1873-5061
DOI	10.1016/j.scr.2020.101917
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: Generation of two induced pluripotent stem cells lines from Mucopolysaccharydosis IIIA patient: IMEDEAi004-A and IMEDEAi004-B.

Vallejo, Sara / Fleischer, Aarne / Martín, José María / Sánchez, Almudena / Palomino, Esther / Bachiller, Daniel

Stem cell research

2018 Volume 32, Page(s) 110–114

Abstract: Mucoplysaccharydosis IIIA (MPSIIIA) is the most severe form of Sanfilippo syndrome. Skin fibroblasts from a MPSIIIA compound heterozygous (E447K/R245H) patient were nucleofected with four OriP/EBNA1-based episomal plasmids containing: OCT3/4, SOX2, KLF4, ...

Abstract	Mucoplysaccharydosis IIIA (MPSIIIA) is the most severe form of Sanfilippo syndrome. Skin fibroblasts from a MPSIIIA compound heterozygous (E447K/R245H) patient were nucleofected with four OriP/EBNA1-based episomal plasmids containing: OCT3/4, SOX2, KLF4, L-Myc, LIN28, BCL-xL and shp53. The two iPSCs lines generated carry both sulfamidase enzyme (SGSH) mutations, are free of plasmid integration, have normal karyotype, express pluripotency-associated markers and are able to differentiate into the three germ layers.
MeSH term(s)	Cell Differentiation/genetics ; Cell Differentiation/physiology ; Cells, Cultured ; Cellular Reprogramming/genetics ; Cellular Reprogramming/physiology ; Humans ; Induced Pluripotent Stem Cells/cytology ; Karyotype ; Mucopolysaccharidosis III/metabolism
Language	English
Publishing date	2018-09-22
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ISSN	1876-7753
ISSN (online)	1876-7753
DOI	10.1016/j.scr.2018.09.009
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: Generation of two induced pluripotent stem cells lines from a Mucopolysaccharydosis IIIB (MPSIIIB) patient.

Vallejo-Diez, Sara / Fleischer, Aarne / Martín-Fernández, José María / Sánchez-Gilabert, Almudena / Bachiller, Daniel

Stem cell research

2018 Volume 33, Page(s) 180–184

Abstract: Mucopolysaccharydosis IIIB is the second most frequent form of Sanfilippo syndrome, a degenerative, pediatric lysosomal storage disease (LSD) characterized by severe neurological disorders and death. We have generated two iPSCs lines derived from dermal ... ...

Abstract	Mucopolysaccharydosis IIIB is the second most frequent form of Sanfilippo syndrome, a degenerative, pediatric lysosomal storage disease (LSD) characterized by severe neurological disorders and death. We have generated two iPSCs lines derived from dermal fibroblast from a MPSIIIB homozygous (P358L) donor. Cells were reprogrammed with OriP/EBNA1-based episomal plasmids containing: OCT3/4, SOX2, KLF4, L-MYC, LIN28, BCL-xL and shp53. Both cell lines are homozygous for the P358L mutation of the α-N-acetylglucosaminidase (NAGLU) gene, have normal karyotype, are free of plasmid integration, express high levels of pluripotency-associated markers and can differentiate into the three germ layers. RESOURCE TABLE: RESOURCE UTILITY: Although the generation of iPSCs has been reported for some lysosomal storage diseases (LSD) in general, and from other mutations of the NAGLU gene in particular (Lemonnier et al., 2011), this is the first time that NAGLU Pro358Leu MPSIIIB-iPSCs lines have been generated and fully characterized demonstrating their quality as iPS cells. RESOURCE DETAILS: Mucopolysaccharidosis IIIB (MPSIII, Sanfilippo syndrome type B) is a pediatric neurodegenerative disorder caused by a deficiency in NAGLU, an enzyme required for lysosomal degradation of heparin sulphate (HS). When the enzyme is absent or malfunctioning, HS accumulates in the cells of several tissues, with devastating effects in the brain and central nervous system. MPSIIIB is inherited in an autosomal recessive manner and presents an incidence between 0.03 and 0.78 cases per 1 × 10
MeSH term(s)	Child, Preschool ; Female ; Humans ; Induced Pluripotent Stem Cells/metabolism ; Mucopolysaccharidosis III/genetics
Language	English
Publishing date	2018-11-01
Publishing country	England
Document type	Case Reports ; Journal Article ; Research Support, Non-U.S. Gov't
ISSN	1876-7753
ISSN (online)	1876-7753
DOI	10.1016/j.scr.2018.10.019
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article: iPSC-Derived Intestinal Organoids from Cystic Fibrosis Patients Acquire CFTR Activity upon TALEN-Mediated Repair of the p.F508del Mutation.

Fleischer, Aarne / Vallejo-Díez, Sara / Martín-Fernández, José María / Sánchez-Gilabert, Almudena / Castresana, Mónica / Del Pozo, Angel / Esquisabel, Amaia / Ávila, Silvia / Castrillo, José Luis / Gaínza, Eusebio / Pedraz, José Luis / Viñas, Miguel / Bachiller, Daniel

Molecular therapy. Methods & clinical development

2020 Volume 17, Page(s) 858–870

Abstract: Cystic fibrosis (CF) is the main genetic cause of death among the Caucasian population. The disease is characterized by abnormal fluid and electrolyte mobility across secretory epithelia. The first manifestations occur within hours of birth (meconium ... ...

Abstract	Cystic fibrosis (CF) is the main genetic cause of death among the Caucasian population. The disease is characterized by abnormal fluid and electrolyte mobility across secretory epithelia. The first manifestations occur within hours of birth (meconium ileus), later extending to other organs, generally affecting the respiratory tract. It is caused by mutations in the cystic fibrosis transmembrane conductance regulator (
Language	English
Publishing date	2020-04-18
Publishing country	United States
Document type	Journal Article
ZDB-ID	2872938-9
ISSN	2329-0501 ; 2329-0501
ISSN (online)	2329-0501
ISSN	2329-0501
DOI	10.1016/j.omtm.2020.04.005
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Article ; Online: New Bicistronic TALENs Greatly Improve Genome Editing.

Martín-Fernández, José María / Fleischer, Aarne / Vallejo-Diez, Sara / Palomino, Esther / Sánchez-Gilabert, Almudena / Ruiz, Raúl / Bejarano, Yazmine / Llinàs, Pere / Gayá, Antoni / Bachiller, Daniel

Current protocols in stem cell biology

2020 Volume 52, Issue 1, Page(s) e104

Abstract: Genome editing has become one of the most powerful tools in present-day stem cell and regenerative medicine research, but despite its rapid acceptance and widespread use, some elements of the technology still need improvement. In this unit, we present ... ...

Abstract	Genome editing has become one of the most powerful tools in present-day stem cell and regenerative medicine research, but despite its rapid acceptance and widespread use, some elements of the technology still need improvement. In this unit, we present data regarding the use of a new, more efficient type of transcription activator-like effector nuclease (TALEN) for gene editing. Our group has generated bicistronic genes in which classical TALEN coding sequences are linked by 2A elements to different reporter molecules, such as fluorochromes (TALEN-F) or membrane receptors (TALEN-M). This structure results in two proteins transcribed from the same transcript, of which the second (the reporter) can be used as the target for selection by fluorescence-assisted cell sorting (FACS) or magnetic-activated cell sorting (MACS). The application of these new TALEN genes allows a rapid enrichment of cells in which both members of the TALEN pair are active, thus eliminating the need for lengthy selection in culture and laborious characterization of a large number of clones. © 2020 by John Wiley & Sons, Inc. Basic Protocol 1: Generation of new TALENs Basic Protocol 2: Genome editing using TALEN-F Alternate Protocol 1: Generation of TALEN-M Support Protocol 1: mRNA in vitro transcription (IVT) of TALEN-T2A-reporter expression vector Alternate Protocol 2: Editing of primary T cells using TALEN-M Basic Protocol 3: Verifying gene editing Support Protocol 2: Rapid expansion protocol for edited T-cells.
MeSH term(s)	Cell Proliferation ; Cloning, Molecular ; Gene Editing/methods ; Genetic Vectors/metabolism ; Humans ; Plasmids/genetics ; RNA, Messenger/genetics ; RNA, Messenger/metabolism ; Reproducibility of Results ; T-Lymphocytes/metabolism ; Transcription Activator-Like Effector Nucleases/metabolism ; Transcription, Genetic
Chemical Substances	RNA, Messenger ; Transcription Activator-Like Effector Nucleases (EC 3.1.-)
Language	English
Publishing date	2020-02-05
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ISSN	1938-8969
ISSN (online)	1938-8969
DOI	10.1002/cpsc.104
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Book ; Online ; Thesis: Implication of Bcl-2 family members in apoptosis signalling pathways triggered by growth factor deprivation

Fleischer, Aarne [Verfasser]

2003

Author's details	vorgelegt von Aarne Fleischer
Keywords	Medizin, Gesundheit ; Medicine, Health
Subject code	sg610
Language	English
Document type	Book ; Online ; Thesis
Database	Digital theses on the web

Full text online

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Article: Role of PATJ in stroke prognosis by modulating endothelial to mesenchymal transition through the Hippo/Notch/PI3K axis.

Medina-Dols, Aina / Cañellas, Guillem / Capó, Toni / Solé, Montse / Mola-Caminal, Marina / Cullell, Natalia / Jaume, Marina / Nadal-Salas, Laura / Llinàs, Jaume / Gómez, Lluis / Tur, Silvia / Jiménez, Carmen / Díaz, Rosa M / Carrera, Caty / Muiño, Elena / Gallego-Fabrega, Cristina / Soriano-Tárraga, Carolina / Ruiz-Guerra, Laura / Pol-Fuster, Josep /

Asensio, Víctor / Muncunill, Josep / Fleischer, Aarne / Iglesias, Amanda / Giralt-Steinhauer, Eva / Lazcano, Uxue / Fernández-Pérez, Isabel / Jiménez-Balado, Joan / Gabriel-Salazar, Marina / Garcia-Gabilondo, Miguel / Lei, Ting / Torres-Aguila, Nuria-Paz / Cárcel-Márquez, Jara / Lladó, Jerònia / Olmos, Gabriel / Rosell, Anna / Montaner, Joan / Planas, Anna M / Rabionet, Raquel / Hernández-Guillamon, Mar / Jiménez-Conde, Jordi / Fernández-Cadenas, Israel / Vives-Bauzá, Cristòfol

Cell death discovery

2024 Volume 10, Issue 1, Page(s) 85

Abstract: Through GWAS studies we identified PATJ associated with functional outcome after ischemic stroke (IS). The aim of this study was to determine PATJ role in brain endothelial cells (ECs) in the context of stroke outcome. PATJ expression analyses in patient' ...

Abstract	Through GWAS studies we identified PATJ associated with functional outcome after ischemic stroke (IS). The aim of this study was to determine PATJ role in brain endothelial cells (ECs) in the context of stroke outcome. PATJ expression analyses in patient's blood revealed that: (i) the risk allele of rs76221407 induces higher expression of PATJ, (ii) PATJ is downregulated 24 h after IS, and (iii) its expression is significantly lower in those patients with functional independence, measured at 3 months with the modified Rankin scale ((mRS) ≤2), compared to those patients with marked disability (mRS = 4-5). In mice brains, PATJ was also downregulated in the injured hemisphere at 48 h after ischemia. Oxygen-glucose deprivation and hypoxia-dependent of Hypoxia Inducible Factor-1α also caused PATJ depletion in ECs. To study the effects of PATJ downregulation, we generated PATJ-knockdown human microvascular ECs. Their transcriptomic profile evidenced a complex cell reprogramming involving Notch, TGF-ß, PI3K/Akt, and Hippo signaling that translates in morphological and functional changes compatible with endothelial to mesenchymal transition (EndMT). PATJ depletion caused loss of cell-cell adhesion, upregulation of metalloproteases, actin cytoskeleton remodeling, cytoplasmic accumulation of the signal transducer C-terminal transmembrane Mucin 1 (MUC1-C) and downregulation of Notch and Hippo signaling. The EndMT phenotype of PATJ-depleted cells was associated with the nuclear recruitment of MUC1-C, YAP/TAZ, β-catenin, and ZEB1. Our results suggest that PATJ downregulation 24 h after IS promotes EndMT, an initial step prior to secondary activation of a pro-angiogenic program. This effect is associated with functional independence suggesting that activation of EndMT shortly after stroke onset is beneficial for stroke recovery.
Language	English
Publishing date	2024-02-17
Publishing country	United States
Document type	Journal Article
ISSN	2058-7716
ISSN	2058-7716
DOI	10.1038/s41420-024-01857-z
Database	MEDical Literature Analysis and Retrieval System OnLINE

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