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  1. Article ; Online: Unequivocal Identification of Aspartic Acid and

    Hui, John O / Flick, Tawnya / Loo, Joseph A / Campuzano, Iain D G

    Journal of the American Society for Mass Spectrometry

    2021  Volume 32, Issue 8, Page(s) 1901–1909

    Abstract: Aspartic acid (Asp) ... ...

    Abstract Aspartic acid (Asp) to
    MeSH term(s) Antibodies, Monoclonal/analysis ; Antibodies, Monoclonal/chemistry ; Aspartic Acid/analysis ; Aspartic Acid/chemistry ; Complementarity Determining Regions/chemistry ; Isoaspartic Acid/analysis ; Isoaspartic Acid/chemistry ; Peptides/analysis ; Peptides/chemistry ; Peptides/standards ; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization/methods ; Tandem Mass Spectrometry
    Chemical Substances Antibodies, Monoclonal ; Complementarity Determining Regions ; Isoaspartic Acid ; Peptides ; Aspartic Acid (30KYC7MIAI)
    Language English
    Publishing date 2021-01-03
    Publishing country United States
    Document type Journal Article
    ZDB-ID 1073671-2
    ISSN 1879-1123 ; 1044-0305
    ISSN (online) 1879-1123
    ISSN 1044-0305
    DOI 10.1021/jasms.0c00370
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 4618: Show issues Location:
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  2. Article ; Online: High-Resolution Demultiplexing (HRdm) Ion Mobility Spectrometry-Mass Spectrometry for Aspartic and Isoaspartic Acid Determination and Screening.

    Butler, Karen E / Dodds, James N / Flick, Tawnya / Campuzano, Iain D G / Baker, Erin S

    Analytical chemistry

    2022  Volume 94, Issue 16, Page(s) 6191–6199

    Abstract: Isomeric peptide analyses are an analytical challenge of great importance to therapeutic monoclonal antibody and other biotherapeutic product development workflows. Aspartic acid (Asp, D) to isoaspartic acid (isoAsp, isoD) isomerization is a critical ... ...

    Abstract Isomeric peptide analyses are an analytical challenge of great importance to therapeutic monoclonal antibody and other biotherapeutic product development workflows. Aspartic acid (Asp, D) to isoaspartic acid (isoAsp, isoD) isomerization is a critical quality attribute (CQA) that requires careful control, monitoring, and quantitation during the drug discovery and production processes. While the formation of isoAsp has been implicated in a variety of disease states such as autoimmune diseases and several types of cancer, it is also understood that the formation of isoAsp results in a structural change impacting efficacy, potency, and immunogenic properties, all of which are undesirable. Currently, lengthy ultrahigh-performance liquid chromatography (UPLC) separations are coupled with MS for CQA analyses; however, these measurements often take over an hour and drastically limit analysis throughput. In this manuscript, drift tube ion mobility spectrometry-mass spectrometry (DTIMS-MS) and both a standard and high-resolution demultiplexing approach were utilized to study eight isomeric Asp and isoAsp peptide pairs. While the limited resolving power associated with the standard DTIMS analysis only separated three of the eight pairs, the application of HRdm distinguished seven of the eight and was only unable to separate DL and isoDL. The rapid high-throughput HRdm DTIMS-MS method was also interfaced with both flow injection and an automated solid phase extraction system to present the first application of HRdm for isoAsp and Asp assessment and demonstrate screening capabilities for isomeric peptides in complex samples, resulting in a workflow highly suitable for biopharmaceutical research needs.
    MeSH term(s) Chromatography, Liquid ; Ion Mobility Spectrometry/methods ; Isoaspartic Acid/analysis ; Mass Spectrometry/methods ; Peptides
    Chemical Substances Isoaspartic Acid ; Peptides
    Language English
    Publishing date 2022-04-14
    Publishing country United States
    Document type Journal Article ; Research Support, U.S. Gov't, Non-P.H.S. ; Research Support, N.I.H., Extramural
    ZDB-ID 1508-8
    ISSN 1520-6882 ; 0003-2700
    ISSN (online) 1520-6882
    ISSN 0003-2700
    DOI 10.1021/acs.analchem.1c05533
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article ; Online: Differentiation of Aspartic and Isoaspartic Acid Using 193 nm Ultraviolet Photodissociation Mass Spectrometry.

    Bashyal, Aarti / Hui, John O / Flick, Tawnya / Dykstra, Andrew B / Zhang, Qingchun / Campuzano, Iain D G / Brodbelt, Jennifer S

    Analytical chemistry

    2023  Volume 95, Issue 30, Page(s) 11510–11517

    Abstract: Spontaneous conversion of aspartic acid (Asp) to isoaspartic acid ( ...

    Abstract Spontaneous conversion of aspartic acid (Asp) to isoaspartic acid (
    MeSH term(s) Isoaspartic Acid/analysis ; Isoaspartic Acid/chemistry ; Amino Acid Sequence ; Mass Spectrometry/methods ; Peptides/chemistry ; Aspartic Acid/chemistry ; Ions ; Ultraviolet Rays
    Chemical Substances Isoaspartic Acid ; Peptides ; Aspartic Acid (30KYC7MIAI) ; Ions
    Language English
    Publishing date 2023-07-17
    Publishing country United States
    Document type Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
    ZDB-ID 1508-8
    ISSN 1520-6882 ; 0003-2700
    ISSN (online) 1520-6882
    ISSN 0003-2700
    DOI 10.1021/acs.analchem.3c02025
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    Zs.A 4033: Show issues Location:
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  4. Book ; Thesis: Neuere Ergebnisse der Histaminforschung

    Flick, Thomas

    e. literar. Analyse

    1983  

    Size 139 S.
    Document type Book ; Thesis
    Thesis / German Habilitation thesis Bonn, Univ., Diss., 1983
    HBZ-ID HT002606399
    Database Catalogue ZB MED Medicine, Health

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    Shelf markLoan statusLocation
    83 D 1844 order for loan Magazin

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  5. Article: Effects of resource distribution on overwinter foraging by mice in agricultural fields and the implications for maize volunteer control

    Flick, Tatyana / Brent Danielson

    Agriculture, ecosystems & environment. 2017 Oct. 01, v. 248

    2017  

    Abstract: Seed predation may be an important ecosystem service for controlling glyphosate–resistant crop volunteers, which are a growing management concern in conventional cropping systems that rely heavily on pesticide based management. In the Midwest USA, ... ...

    Abstract Seed predation may be an important ecosystem service for controlling glyphosate–resistant crop volunteers, which are a growing management concern in conventional cropping systems that rely heavily on pesticide based management. In the Midwest USA, prairie deer mice (Peromyscus maniculatus) are important seed predators, removing unwanted weed seeds and waste grain from the soil surface all year round. In this study, we examined how the spatial distribution of experimental maize (Zea mays) seed patches influenced overwinter foraging, mouse populations and waste grain removal in conventional crop fields. We predicted that (1) individual mice will increase foraging on maize waste grain in fields with many small maize seed patches compared to fields with a few large maize seed patches (functional response) and (2) more mice will forage in fields with many small seed patches than in fields with a few large seed patches (numerical response). We found mouse functional responses were not influenced by the spatial distribution of maize seed patches nor did mice respond numerically to seed addition in general. Mice did, however, remove 59–66% (94–106kgha−1) of the maize seeds, providing a valuable ecosystem service. Our work shows that prairie deer mice are able to remove large amounts of waste grain from the soil surface over winter, supporting the hypothesis that overwinter seed predation by mice can drastically reduce volunteer maize densities in conventional crop fields. Future research should further examine what management practices (such as practicing no-till) increase mouse abundance and foraging efficacy, so that management strategies that maximize seed predation can be developed.
    Keywords corn ; cropping systems ; ecosystem services ; foraging ; glyphosate ; granivores ; herbicide resistance ; mice ; no-tillage ; Peromyscus maniculatus ; seed predation ; seeds ; soil ; wastes ; weeds ; winter ; Zea mays ; Midwestern United States
    Language English
    Dates of publication 2017-1001
    Size p. 82-87.
    Publishing place Elsevier B.V.
    Document type Article
    ZDB-ID 602345-9
    ISSN 1873-2305 ; 0167-8809
    ISSN (online) 1873-2305
    ISSN 0167-8809
    DOI 10.1016/j.agee.2017.07.025
    Database NAL-Catalogue (AGRICOLA)

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    In stock of ZB MED Bonn / Germany

    Z 3803: Show issues
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  6. Book ; Online: A novel approach for FPGA-to-server data transmission over an Ethernet-based network using the eXpress Data Path technology

    Dülsen, Carsten / Flick, Tobias / Göhring, Timo / Wagner, Wolfgang / Wensing, Marius

    2022  

    Abstract: In the context of the upgrade of the Large Hadron Collider at CERN for high-luminosity operation, the particle detectors have to cope with much higher data rates and therefore need to upgrade their data acquisition systems. This upgrade is taken as an ... ...

    Abstract In the context of the upgrade of the Large Hadron Collider at CERN for high-luminosity operation, the particle detectors have to cope with much higher data rates and therefore need to upgrade their data acquisition systems. This upgrade is taken as an opportunity to exchange the currently used highly customized hardware by commercial solutions. Nevertheless, some part of the data processing still needs to be done within Field Programmable Gate Arrays (FPGA), requiring the transfer of data between the FPGAs and the commercial servers. This paper reports on a study of direct data transmission from FPGAs to servers via a commercial network. Large data buffers as required for reliable data-transmission protocols are avoided by using an emerging technique named eXpress Data Path (XDP). Based on XDP, the transmission of 5.2 PB (i.e. 2.92 * 10^{12} packets) was achieved within 168 h without a single missing packet.

    Comment: 12 pages, 9 figures
    Keywords Computer Science - Networking and Internet Architecture
    Subject code 006
    Publishing date 2022-08-15
    Publishing country us
    Document type Book ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  7. Article ; Online: Electrophoretic Desalting To Improve Performance in Electrospray Ionization Mass Spectrometry.

    Zhang, Zezhen / Pulliam, Christopher J / Flick, Tawnya / Cooks, R Graham

    Analytical chemistry

    2018  Volume 90, Issue 6, Page(s) 3856–3862

    Abstract: Mass spectrometers are sensitive tools used to identify and quantify both small and large analytes using the mass-to-charge ratios ( m/ z) of ions generated by electrospray ionization (ESI) or other methods. Ionization typically generates protonated or ... ...

    Abstract Mass spectrometers are sensitive tools used to identify and quantify both small and large analytes using the mass-to-charge ratios ( m/ z) of ions generated by electrospray ionization (ESI) or other methods. Ionization typically generates protonated or deprotonated forms of the analytes or adducts with adventitious metal ions derived from the spray solvent. The formation of a variety of ionized forms of the analyte as well as the presence of cluster ions complicates the data and can have deleterious effects on the performance of the mass spectrometer, especially under high salt or buffer conditions. To address this, a method involving a dual-electrode nano-electrospray source has been implemented to rapidly and temporarily desalt the spray solution of interfering cationic and anionic species using electrophoretic transport from the spray tip. Peptides, proteins, and pharmaceutical drugs all showed improved results after the desalting process as measured by the quality of the mass spectra and the limits of detection achieved. Importantly ordinary phosphate buffers could be used to record protein mass spectra by nano-ESI.
    Language English
    Publishing date 2018-02-28
    Publishing country United States
    Document type Journal Article ; Research Support, U.S. Gov't, Non-P.H.S. ; Research Support, Non-U.S. Gov't
    ZDB-ID 1508-8
    ISSN 1520-6882 ; 0003-2700
    ISSN (online) 1520-6882
    ISSN 0003-2700
    DOI 10.1021/acs.analchem.7b04529
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 4033: Show issues Location:
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  8. Article: Classification error for a very large number of classes.

    Fukunaga, K / Flick, T E

    IEEE transactions on pattern analysis and machine intelligence

    2012  Volume 6, Issue 6, Page(s) 779–788

    Abstract: Classification error is analyzed for a situation where the number of possible classes may be on the order of a hundred or more. The error associated with classifying to a single class is shown to depend mainly on average nearest-neighbor distance between ...

    Abstract Classification error is analyzed for a situation where the number of possible classes may be on the order of a hundred or more. The error associated with classifying to a single class is shown to depend mainly on average nearest-neighbor distance between class means, noise level, and effective dimensionality of the class mean distribution and not much on other aspects of the distribution, noise correlation, or number of classes. Since single class error is large, separation of classes into groups is also explored. Group classification error has the same properties as single class error but the size of the error is moderated by the Bayes overlap between groups. Standard curves are provided to predict single class and group error. Also discussed are the effect of pattern blurring on classification error and the nearest-neighbor distance statistics throughout a distribution.
    Language English
    Publishing date 2012-04-12
    Publishing country United States
    Document type Journal Article
    ISSN 0162-8828
    ISSN 0162-8828
    DOI 10.1109/tpami.1984.4767601
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  9. Article: A Test of the Gaussian-ness of a Data Set Using Clustering.

    Fukunaga, K / Flick, T E

    IEEE transactions on pattern analysis and machine intelligence

    2011  Volume 8, Issue 2, Page(s) 240–247

    Abstract: The properties of the ``magnifying glass'' method of clustering are discussed. These properties, which include unbiased and consistent estimation of the mean for Gaussian distributions and biased and inconsistent estimation of the mean for non-Gaussian ... ...

    Abstract The properties of the ``magnifying glass'' method of clustering are discussed. These properties, which include unbiased and consistent estimation of the mean for Gaussian distributions and biased and inconsistent estimation of the mean for non-Gaussian distributions, lead to the development of a technique for testing data to determine whether or not it is Gaussian. An example of a non-Gaussian distribution is given to show the sensitivity of the proposed Gaussian test.
    Language English
    Publishing date 2011-08-19
    Publishing country United States
    Document type Journal Article
    ISSN 0162-8828
    ISSN 0162-8828
    DOI 10.1109/tpami.1986.4767777
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  10. Article: Estimation of the parameters of a gaussian mixture using the method of moments.

    Fukunaga, K / Flick, T E

    IEEE transactions on pattern analysis and machine intelligence

    2011  Volume 5, Issue 4, Page(s) 410–416

    Abstract: Given a general n-dimensional bimodal Gaussian mixture, this paper shows how unknown parameters may be found by the method of moments. Three cases are considered-equal modal probabilities, known but not necessarily equal probabilities, and all parameters ...

    Abstract Given a general n-dimensional bimodal Gaussian mixture, this paper shows how unknown parameters may be found by the method of moments. Three cases are considered-equal modal probabilities, known but not necessarily equal probabilities, and all parameters unknown. The solution involves sample moments no higher than fourth order. For Gaussian mixtures where the number of modes is unknown, fourth-order moments can be used to count them, provided all modes have the same covariance matrix, and their multiplicity is not greater than data dimensionality. Examples of mode-counting and the determination of bimodal parameters are included.
    Language English
    Publishing date 2011-08-19
    Publishing country United States
    Document type Journal Article
    ISSN 0162-8828
    ISSN 0162-8828
    DOI 10.1109/tpami.1983.4767410
    Database MEDical Literature Analysis and Retrieval System OnLINE

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