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  1. Article ; Online: iDISCO Tissue Clearing Whole-Brain and Light Sheet Microscopy for High-Throughput Imaging in a Mouse Model of Traumatic Brain Injury.

    Flinn, Hannah / Cruz-Pineda, Leonardo / Montier, Laura / Horner, Philip J / Villapol, Sonia

    Methods in molecular biology (Clifton, N.J.)

    2024  Volume 2761, Page(s) 589–597

    Abstract: Immunolabeling-enabled imaging of solvent-cleared organs (iDISCO) (Renier N, Wu Z, Simon DJ, Yang J, Ariel P, Tessier-Lavigne M, Cell 159:896-910, 2014) aims to match the refractive index (RI) of tissue to the surrounding medium, thereby facilitating ... ...

    Abstract Immunolabeling-enabled imaging of solvent-cleared organs (iDISCO) (Renier N, Wu Z, Simon DJ, Yang J, Ariel P, Tessier-Lavigne M, Cell 159:896-910, 2014) aims to match the refractive index (RI) of tissue to the surrounding medium, thereby facilitating three-dimensional (3D) imaging and quantification of cellular points and tissue structures. Once cleared, transparent tissue samples allow for rapid imaging with no mechanical sectioning. This imaging technology enables us to visualize brain tissue in situ and quantify the morphology and extent of glial cell branches or neuronal processes extending from the epicenter of a traumatic brain injury (TBI). In this way, we can more accurately assess and quantify the damaging consequences of TBI not only in the impact region but also in the extended pericontusional regions.
    MeSH term(s) Mice ; Animals ; Microscopy ; Imaging, Three-Dimensional/methods ; Solvents ; Brain Injuries, Traumatic/diagnostic imaging ; Brain
    Chemical Substances Solvents
    Language English
    Publishing date 2024-03-01
    Publishing country United States
    Document type Journal Article
    ISSN 1940-6029
    ISSN (online) 1940-6029
    DOI 10.1007/978-1-0716-3662-6_39
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article: Probiotic treatment causes sex-specific neuroprotection after traumatic brain injury in mice.

    Holcomb, Morgan / Marshall, Austin / Flinn, Hannah / Lozano, Mariana / Soriano, Sirena / Gomez-Pinilla, Fernando / Treangen, Todd J / Villapol, Sonia

    Research square

    2024  

    Abstract: Background: Recent studies have shed light on the potential role of gut dysbiosis in shaping traumatic brain injury (TBI) outcomes. Changes in the levels and types of : Objective: This study aimed to investigate the effects of a daily pan-probiotic ( ... ...

    Abstract Background: Recent studies have shed light on the potential role of gut dysbiosis in shaping traumatic brain injury (TBI) outcomes. Changes in the levels and types of
    Objective: This study aimed to investigate the effects of a daily pan-probiotic (PP) mixture in drinking water containing strains of
    Methods: Mice were subjected to controlled cortical impact (CCI) injury. Short-chain fatty acids (SCFAs) analysis was performed for metabolite measurements. The taxonomic profiles of murine fecal samples were evaluated using 16S rRNA V1-V3 sequencing analysis. Histological analyses were used to assess neuroinflammation and gut changes post-TBI, while behavioral tests were conducted to evaluate sensorimotor and cognitive functions.
    Results: Our findings suggest that PP administration modulates the diversity and composition of the microbiome and increases the levels of SCFAs in a sex-dependent manner. We also observed a reduction of lesion volume, cell death, and microglial and macrophage activation after PP treatment following TBI in male mice. Furthermore, PP-treated mice show motor function improvements and decreases in anxiety and depressive-like behaviors.
    Conclusion: Our findings suggest that PP administration can mitigate neuroinflammation and ameliorate motor and anxiety and depressive-like behavior deficits following TBI. These results underscore the potential of probiotic interventions as a viable therapeutic strategy to address TBI-induced impairments, emphasizing the need for gender-specific treatment approaches.
    Language English
    Publishing date 2024-04-02
    Publishing country United States
    Document type Preprint
    DOI 10.21203/rs.3.rs-4196801/v1
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article: Sex-dependent improvement in traumatic brain injury outcomes after liposomal delivery of dexamethasone in mice.

    Baudo, Gherardo / Flinn, Hannah / Holcomb, Morgan / Tiwari, Anjana / Soriano, Sirena / Taraballi, Francesca / Godin, Biana / Zinger, Assaf / Villapol, Sonia

    bioRxiv : the preprint server for biology

    2023  

    Abstract: Traumatic Brain Injury (TBI) can have long-lasting physical, emotional, and cognitive consequences due to the neurodegeneration caused by its robust inflammatory response. Despite advances in rehabilitation care, effective neuroprotective treatments for ... ...

    Abstract Traumatic Brain Injury (TBI) can have long-lasting physical, emotional, and cognitive consequences due to the neurodegeneration caused by its robust inflammatory response. Despite advances in rehabilitation care, effective neuroprotective treatments for TBI patients are lacking. Furthermore, current drug delivery methods for TBI treatment are inefficient in targeting inflamed brain areas. To address this issue, we have developed a liposomal nanocarrier (Lipo) encapsulating dexamethasone (Dex), an agonist for the glucocorticoid receptor utilized to alleviate inflammation and swelling in various conditions. In vitro studies show that Lipo-Dex were well tolerated in human and murine neural cells. Lipo-Dex showed significant suppression of inflammatory cytokines, IL-6 and TNF-α, release after induction of neural inflammation with lipopolysaccharide. Further, the Lipo-Dex were administered to young adult male and female C57BL/6 mice immediately after a controlled cortical impact injury. Our findings demonstrate that Lipo-Dex can selectively target the injured brain, thereby reducing lesion volume, cell death, astrogliosis, the release of proinflammatory cytokines, and microglial activation compared to Lipo-treated mice in a sex-dependent manner, showing a major impact only in male mice. This highlights the importance of considering sex as a crucial variable in developing and evaluating new nano-therapies for brain injury. These results suggest that Lipo-Dex administration may effectively treat acute TBI.
    Language English
    Publishing date 2023-05-17
    Publishing country United States
    Document type Preprint
    DOI 10.1101/2023.05.16.541045
    Database MEDical Literature Analysis and Retrieval System OnLINE

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