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  1. Article ; Online: Use of soybean as an alternative protein source for welfare-orientated production of American alligators (

    Flint, Mark / Flint, Jaylene

    PeerJ

    2023  Volume 11, Page(s) e16321

    Abstract: Soybean meal based diets have been suggested to cause gastrointestinal issues in certain species when used as a protein alternative. Using a randomized design, we tested 1,728 alligators in one of 13 communal pens offered one of two diets (seven pens ( ...

    Abstract Soybean meal based diets have been suggested to cause gastrointestinal issues in certain species when used as a protein alternative. Using a randomized design, we tested 1,728 alligators in one of 13 communal pens offered one of two diets (seven pens (
    MeSH term(s) Animals ; Glycine max/chemistry ; Alligators and Crocodiles ; Soybean Proteins ; Diet/veterinary ; Minerals
    Chemical Substances Soybean Proteins ; Minerals
    Language English
    Publishing date 2023-10-26
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2703241-3
    ISSN 2167-8359 ; 2167-8359
    ISSN (online) 2167-8359
    ISSN 2167-8359
    DOI 10.7717/peerj.16321
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: Field Based Assessment of Clinical Signs of Irreversible Loss of Consciousness and Death Confirmed by Brain Destruction in Juvenile American Alligators (

    Flint, M / Sagrera, K / Wainwright, K / Flint, J B

    Journal of applied animal welfare science : JAAWS

    2023  , Page(s) 1–12

    Abstract: To determine the humane use of slaughter methods we examined the clinical signs of life in 61 American alligators harvested on-farm using one of three methods: (i) captive bolt and spinal cord severance; (ii) electrostunning, spinal cord severance and ... ...

    Abstract To determine the humane use of slaughter methods we examined the clinical signs of life in 61 American alligators harvested on-farm using one of three methods: (i) captive bolt and spinal cord severance; (ii) electrostunning, spinal cord severance and pithing; and (iii) spinal cord severance and pithing. Loss of consciousness and the six clinical signs of life that can be used on-farm were assessed for evidence of irreversible unconsciousness and death at Time 0, 0.5, 1, 2, 5, 10, 20, and 30 min post slaughter. The brains of alligators from each slaughter method were removed to assess brain tissue disruption. A combination of loss of blink reflex, pupillary light response, jaw tone and respiration are a reliable on-farm tool for determining death. Heartbeat and withdrawal reflex persisted. Captive bolt and electrostunning methods were effective in immediately producing loss of response consistent with irreversible unconsciousness, subsequent death and destruction of neural tissue integrity in the mid and hind brain. They are therefore humane forms of slaughter in American alligators.
    Language English
    Publishing date 2023-08-03
    Publishing country England
    Document type Journal Article
    ISSN 1532-7604
    ISSN (online) 1532-7604
    DOI 10.1080/10888705.2023.2236550
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article ; Online: Techniques to assess reproductive status in adult female American alligators (Alligator mississippiensis) using laparoscopic examination.

    Flint, Mark / Flint, Jaylene B / Miller, Jeffrey D

    PloS one

    2023  Volume 18, Issue 11, Page(s) e0287140

    Abstract: This protocol describes a minimally invasive surgical technique and approach to successfully examine the gonads of live female American alligators as part of a reproductive examination used in conservation medicine and biology. Best practices are based ... ...

    Abstract This protocol describes a minimally invasive surgical technique and approach to successfully examine the gonads of live female American alligators as part of a reproductive examination used in conservation medicine and biology. Best practices are based on examination of over 80 American alligators in the last two years adapting principles derived from other reptilian megafauna species. This protocol is designed for appropriately qualified veterinarians and biologists working in the field. We show likely reproductive tract presentations with respect to breeding status and environmental cues to help guide interpretation of observations. The laparoscopic approach and findings presented here provide tools to safely clinically examine animals in a welfare-oriented way that will advance our understanding of crocodilian reproduction. This technique has not previously been described in this species.
    MeSH term(s) Animals ; Female ; Alligators and Crocodiles ; Reproduction ; Gonads ; Medicine ; Genitalia
    Language English
    Publishing date 2023-11-15
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2267670-3
    ISSN 1932-6203 ; 1932-6203
    ISSN (online) 1932-6203
    ISSN 1932-6203
    DOI 10.1371/journal.pone.0287140
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article: Prevention over screening for ovarian cancer in patients with high-risk germline mutations: Misinterpreting the findings of ALDO.

    Manning-Geist, Beryl L / Flint, Matthew / Roche, Kara Long

    Gynecologic oncology reports

    2023  Volume 46, Page(s) 101157

    Abstract: In November 2022, the findings of the Avoiding Late Diagnosis of Ovarian cancer (ALDO) study were published. Subsequent media coverage suggested that investigators had found a safe alternative to risk-reducing bilateral salpingoophorectomy (rrBSO) in ... ...

    Abstract In November 2022, the findings of the Avoiding Late Diagnosis of Ovarian cancer (ALDO) study were published. Subsequent media coverage suggested that investigators had found a safe alternative to risk-reducing bilateral salpingoophorectomy (rrBSO) in patients with pathogenic
    Language English
    Publishing date 2023-03-02
    Publishing country Netherlands
    Document type Journal Article
    ZDB-ID 2818505-5
    ISSN 2352-5789
    ISSN 2352-5789
    DOI 10.1016/j.gore.2023.101157
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article: Metastatic ovarian carcinosarcoma in a patient undergoing in-vitro fertilization: A case report.

    Flint, Matthew / Velasquez, Jessica / Carr, Caitlin / Kolev, Valentin / Zakashansky, Konstantin

    International journal of surgery case reports

    2023  Volume 104, Page(s) 107937

    Abstract: Introduction and importance: Ovarian carcinosarcomas (OCS) are highly aggressive tumors containing both carcinomatous and sarcomatous elements. Patients are typically older postmenopausal women who present with advanced disease, however rarely young ... ...

    Abstract Introduction and importance: Ovarian carcinosarcomas (OCS) are highly aggressive tumors containing both carcinomatous and sarcomatous elements. Patients are typically older postmenopausal women who present with advanced disease, however rarely young women can be affected.
    Case presentation: A 41-year-old woman undergoing fertility treatment was found to have a new 9-10 cm pelvic mass on routine transvaginal ultrasound (TVUS) 16 days after embryo transfer. Diagnostic laparoscopy revealed a mass in the posterior cul-de-sac that was surgically excised and sent to pathology for evaluation. Pathology was consistent with carcinosarcoma of gynecologic origin. Further work-up revealed advanced disease with apparent rapid progression. Patient underwent interval debulking surgery after four cycles of neoadjuvant chemotherapy with carboplatin and paclitaxel with final pathology consistent with primary ovarian carcinosarcoma and complete gross resection of disease.
    Clinical discussion: In the setting of advanced disease neoadjuvant chemotherapy with a platinum-based chemotherapy regimen followed by cytoreductive surgery is a standard approach to treatment of OCS. Given the rarity of disease, most data regarding treatment has been extrapolated from other forms of epithelial ovarian cancer. Specific risk factors for disease development of OCS including the long-term effects of assisted reproductive technology remain understudied.
    Conclusion: While OCS are rare highly aggressive biphasic tumors that primarily affect older postmenopausal woman, we present a unique case of OCS incidentally found in a young woman undergoing fertility treatment via in-vitro fertilization.
    Language English
    Publishing date 2023-02-17
    Publishing country Netherlands
    Document type Case Reports
    ISSN 2210-2612
    ISSN 2210-2612
    DOI 10.1016/j.ijscr.2023.107937
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  6. Article ; Online: Novel plant-based meat alternatives: future opportunities and health considerations.

    Flint, Megan / Bowles, Simon / Lynn, Anthony / Paxman, Jenny R

    The Proceedings of the Nutrition Society

    2023  Volume 82, Issue 3, Page(s) 370–385

    Abstract: Present food systems threaten population and environmental health. Evidence suggests reduced meat and increased plant-based food consumption would align with climate change and health promotion priorities. Accelerating this transition requires greater ... ...

    Abstract Present food systems threaten population and environmental health. Evidence suggests reduced meat and increased plant-based food consumption would align with climate change and health promotion priorities. Accelerating this transition requires greater understanding of determinants of plant-based food choice. A thriving plant-based food industry has emerged to meet consumer demand and support dietary shift towards plant-based eating. 'Traditional' plant-based diets are low-energy density, nutrient dense, low in saturated fat and purportedly associated with health benefits. However, fast-paced contemporary lifestyles continue to fuel growing demand for meat-mimicking plant-based convenience foods which are typically ultra-processed. Processing can improve product safety and palatability and enable fortification and enrichment. However, deleterious health consequences have been associated with ultra-processing, though there is a paucity of equivocal evidence regarding the health value of novel plant-based meat alternatives (PBMAs) and their capacity to replicate the nutritional profile of meat-equivalents. Thus, despite the health halo often associated with plant-based eating, there is a strong rationale to improve consumer literacy of PBMAs. Understanding the impact of extensive processing on health effects may help to justify the use of innovative methods designed to maintain health benefits associated with particular foods and ingredients. Furthering knowledge regarding the nutritional value of novel PBMAs will increase consumer awareness and thus support informed choice. Finally, knowledge of factors influencing engagement of target consumer subgroups with such products may facilitate production of desirable, healthier PBMAs. Such evidence-based food manufacturing practice has the potential to positively influence future individual and planetary health.
    MeSH term(s) Humans ; Meat ; Diet ; Food Preferences/psychology ; Food Industry ; Food-Processing Industry
    Language English
    Publishing date 2023-01-06
    Publishing country England
    Document type Journal Article ; Review ; Research Support, Non-U.S. Gov't
    ZDB-ID 391142-1
    ISSN 1475-2719 ; 0029-6651
    ISSN (online) 1475-2719
    ISSN 0029-6651
    DOI 10.1017/S0029665123000034
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  7. Article ; Online: Effects of time to treatment initiation on outcomes for soft tissue sarcomas.

    Ryu, Jae Hong / Rahman, Juma / Deo, Shaneel / Flint, Michael

    Journal of surgical oncology

    2023  Volume 127, Issue 7, Page(s) 1174–1186

    Abstract: Background and objectives: Few studies have investigated the effects of time to treatment initiation (TTI) for soft tissue sarcomas (STS). Our objective was to investigate the risk factors for prolonged TTI and the effects of prolonged TTI on local ... ...

    Abstract Background and objectives: Few studies have investigated the effects of time to treatment initiation (TTI) for soft tissue sarcomas (STS). Our objective was to investigate the risk factors for prolonged TTI and the effects of prolonged TTI on local recurrence free survival (LRFS), distant metastasis free survival (DMFS), and disease specific survival (DSS).
    Method: Patients diagnosed with high-grade STS of the extremities and trunk from 2011 to 2020 were included. TTI was grouped into two groups (treatment provided in less than vs. more than or equal to 30 days). Two-year and 5-year survival probabilities were calculated for LRFS, DMFS, and DSS. Cox regression and Kruskal-Wallis tests in univariate analysis were conducted to find risk factors affecting TTI and the survival outcomes.
    Results: In the univariate analysis, diagnosis in the later 5-year period of the study, tumor size, and treatment modality were associated with prolonged TTI. TTI ≥30 days was associated with higher DMFS but no association was found with LRFS or DSS. Tumor size, surgical margins, and provision of surgery were associated with DSS.
    Conclusion: Despite the delay in treatment for STS patients caused by the COVID-19 pandemic, our study showed TTI of more than 30 days does not negatively impact patients.
    MeSH term(s) Humans ; Time-to-Treatment ; Pandemics ; Retrospective Studies ; COVID-19 ; Sarcoma/pathology ; Extremities/pathology ; Soft Tissue Neoplasms/surgery ; Neoplasm Recurrence, Local/pathology ; Prognosis
    Language English
    Publishing date 2023-03-20
    Publishing country United States
    Document type Journal Article
    ZDB-ID 82063-5
    ISSN 1096-9098 ; 0022-4790
    ISSN (online) 1096-9098
    ISSN 0022-4790
    DOI 10.1002/jso.27237
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  8. Article ; Online: Using ecosystem health and welfare assessments to determine impacts of wild collection for public aquariums.

    Fischer, Brittany / Pempek, Jessica / George, Kelly Ann / Flint, Jaylene / Wittum, Thomas / Flint, Mark

    PloS one

    2023  Volume 18, Issue 5, Page(s) e0285198

    Abstract: Aquatic ecosystems are currently facing a multitude of stressors from anthropogenic impacts, including climate change, pollution, and overfishing. Public aquariums positively contribute to ecosystems through conservation, education, and scientific ... ...

    Abstract Aquatic ecosystems are currently facing a multitude of stressors from anthropogenic impacts, including climate change, pollution, and overfishing. Public aquariums positively contribute to ecosystems through conservation, education, and scientific advancement; but may also negatively detract from these systems through collection of animals from the wild and sourcing from commercial suppliers. Changes within the industry have occurred, although evidence-based assessments of 1) how aquariums collect and maintain their populations to determine sustainability of the environment they have harvested; and 2) the welfare of these harvested animals once within the aquariums are still needed. The objectives of this study were to assess the ecosystem health of locations aquariums frequently visit to collect fish from the wild, and then evaluate the wellbeing of fishes at aquariums after extended periods in captivity. Assessments included use of chemical, physical, and biological indicators at field sites, and use of a quantitative welfare assessment at aquariums for comparison to species reared through aquaculture. Anthropogenic pressures at field sites were observed, but no evidence of high degradation or compromised health of animals were found. Welfare assessments of aquarium exhibit tanks produced high-positive scores overall (> 70/84), demonstrating that both wild collected (avg. score 78.8) and aquaculture fishes (avg. score 74.5) were coping appropriately within their environments. Although findings indicated that fish can be taken from the wild at low-moderate rates without any deleterious impact on the environment and cope equally well in aquarium settings, alternatives such as aquaculture should be considered as a strategy to reduce pressure on known stressed aquatic environments or where significant numbers of fishes are being taken.
    MeSH term(s) Animals ; Ecosystem ; Conservation of Natural Resources ; Fisheries ; Fishes ; Aquaculture
    Language English
    Publishing date 2023-05-02
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2267670-3
    ISSN 1932-6203 ; 1932-6203
    ISSN (online) 1932-6203
    ISSN 1932-6203
    DOI 10.1371/journal.pone.0285198
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  9. Article: Mixed squamous and clear cell ovarian adenocarcinoma arising from endometriosis in a 71 year old patient.

    Mills, Alexandra / Saleh, Mona / Erdem Kombak, Faruk / Flint, Matthew / Kolev, Valentin

    Gynecologic oncology reports

    2023  Volume 48, Page(s) 101225

    Abstract: This 71 year old patient was diagnosed with mixed squamous and clear cell ovarian adenocarcinoma.•Patient was surgically staged with guidance from frozen section.•Patient received adjuvant treatment with carboplatin and paclitaxel for 6 cycles. ...

    Abstract •This 71 year old patient was diagnosed with mixed squamous and clear cell ovarian adenocarcinoma.•Patient was surgically staged with guidance from frozen section.•Patient received adjuvant treatment with carboplatin and paclitaxel for 6 cycles.
    Language English
    Publishing date 2023-06-22
    Publishing country Netherlands
    Document type Case Reports
    ZDB-ID 2818505-5
    ISSN 2352-5789
    ISSN 2352-5789
    DOI 10.1016/j.gore.2023.101225
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  10. Article ; Online: Program evaluation of a school-based mental health and wellness curriculum featuring yoga and mindfulness.

    McCurdy, Bethany H / Bradley, Travis / Matlow, Ryan / Rettger, John P / Espil, Flint M / Weems, Carl F / Carrion, Victor G

    PloS one

    2024  Volume 19, Issue 4, Page(s) e0301028

    Abstract: Background: Interest in the effectiveness of mindfulness-based interventions such as yoga in primary schools has grown. Evidence shows promise, as youth who engage in yoga to promote mindfulness show improved coping skills, increased socio-emotional ... ...

    Abstract Background: Interest in the effectiveness of mindfulness-based interventions such as yoga in primary schools has grown. Evidence shows promise, as youth who engage in yoga to promote mindfulness show improved coping skills, increased socio-emotional competence and prosocial skills, academic performance, attention span, and ability to deal with stress.
    Objective: This study reports the results of a program evaluation of a universal health and wellness curriculum, Pure Power, designed to teach youth yoga techniques, mindfulness, and emotion regulation.
    Methods: A non-randomized comparison design examined outcomes among participants from schools that completed the intervention with highest fidelity of implementation (n = 461) and from students in matched comparison schools (n = 420). Standard measures of coping, emotion regulation and emotion dysregulation, spelling, and math achievement were collected.
    Results: Analyses suggest the youth in the intervention schools demonstrated relative improvement on measures of emotion regulation, spelling, and math.
    Conclusions: Challenges in implementation in real-life settings are vital to identify. The data provide some real-world evidence for the effectiveness of a universal health and wellness curriculum on emotion regulation and positive academic outcomes. Training school staff to deliver the intervention may foster implementation. Future research should test the effectiveness of who delivers the intervention; for example, teacher-delivered groups vs. other wellness personnel.
    MeSH term(s) Adolescent ; Humans ; Yoga/psychology ; Mental Health ; Mindfulness/methods ; Program Evaluation ; Schools ; Curriculum
    Language English
    Publishing date 2024-04-04
    Publishing country United States
    Document type Journal Article
    ZDB-ID 2267670-3
    ISSN 1932-6203 ; 1932-6203
    ISSN (online) 1932-6203
    ISSN 1932-6203
    DOI 10.1371/journal.pone.0301028
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