LIVIVO - Search results -

Search results

Result 1 - 10 of total 14

Search options

Article ; Online: UV-C irradiation as an effective tool for sterilization of porcine chimeric VP1-PCV2bCap recombinant vaccine.

Vrablikova, Alena / Fojtikova, Martina / Hezova, Renata / Simeckova, Pavlina / Brezani, Veronika / Strakova, Nicol / Fraiberk, Martin / Kotoucek, Jan / Masek, Josef / Psikal, Ivan

Scientific reports

2023 Volume 13, Issue 1, Page(s) 19337

Abstract: Ultraviolet irradiation is an effective method of virus and bacteria inactivation. The dose of UV-C light necessary for baculovirus inactivation by measurement of fluorescent GFP protein produced by baculovirus expression system after the irradiation of ... ...

Abstract	Ultraviolet irradiation is an effective method of virus and bacteria inactivation. The dose of UV-C light necessary for baculovirus inactivation by measurement of fluorescent GFP protein produced by baculovirus expression system after the irradiation of baculovirus culture in doses ranging from 3.5 to 42 J/m
MeSH term(s)	Mice ; Animals ; Swine ; Vaccines, Synthetic ; Viruses ; Sterilization ; Recombinant Proteins/genetics ; Ultraviolet Rays
Chemical Substances	Vaccines, Synthetic ; Recombinant Proteins
Language	English
Publishing date	2023-11-07
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2615211-3
ISSN	2045-2322 ; 2045-2322
ISSN (online)	2045-2322
ISSN	2045-2322
DOI	10.1038/s41598-023-46791-9
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

Article: Development of modern immunization agent against bovine papillomavirus type 1 infection based on BPV1 L1 recombinant protein.

Vrablikova, Alena / Brezani, Veronika / Psikal, Ivan / Fraiberk, Martin / Sebela, Marek / Fojtikova, Martina / Kulich, Pavel / Hezova, Renata / Masek, Josef

Frontiers in veterinary science

2023 Volume 10, Page(s) 1116661

Abstract: Bovine papillomavirus type 1 L1 protein was produced in a baculovirus expression system and purified as virus-like particles (VLPs) by affinity chromatography using lectins. The morphological integrity of VLPs was confirmed by electron microscopy. ... ...

Abstract	Bovine papillomavirus type 1 L1 protein was produced in a baculovirus expression system and purified as virus-like particles (VLPs) by affinity chromatography using lectins. The morphological integrity of VLPs was confirmed by electron microscopy. Differences between the two detected variants were deciphered by mass spectrometry of peptides (MALDI-TOF). Mice were immunized with purified VLPs in doses of 10, 25, or 50 μg in combination with 1% saponin and 15% alhydrogel per dose as adjuvants. Analysis of the humoral immune response revealed increased levels of specific antibodies detected 3 weeks after the first immunization in all groups of animals. This was further significantly increased by the booster applied 3 weeks after the first dose, with the best immune response in a group of mice immunized by the largest dose of antigen. BPV1 L1 VLPs purified by affinity chromatography using lectins could be used for prophylactic immunization in veterinary medicine.
Language	English
Publishing date	2023-03-28
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2834243-4
ISSN	2297-1769
ISSN	2297-1769
DOI	10.3389/fvets.2023.1116661
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Article ; Online: Viral RNA-Dependent RNA Polymerase Inhibitor 7-Deaza-2'-

Eyer, Luděk / Fojtíková, Martina / Nencka, Radim / Rudolf, Ivo / Hubálek, Zdeněk / Ruzek, Daniel

Antimicrobial agents and chemotherapy

2019 Volume 63, Issue 3

Abstract: West Nile virus (WNV) is a medically important emerging arbovirus causing serious neuroinfections in humans and against which no approved antiviral therapy is currently available. In this study, we demonstrate that 2'- ...

Abstract	West Nile virus (WNV) is a medically important emerging arbovirus causing serious neuroinfections in humans and against which no approved antiviral therapy is currently available. In this study, we demonstrate that 2'-
MeSH term(s)	Animals ; Antiviral Agents/therapeutic use ; Cell Line ; Chlorocebus aethiops ; Disease Models, Animal ; Disease Progression ; Female ; Mice ; Mice, Inbred BALB C ; RNA Replicase/antagonists & inhibitors ; Swine ; Tubercidin/analogs & derivatives ; Tubercidin/therapeutic use ; Vero Cells ; Viremia/drug therapy ; Virus Replication/drug effects ; West Nile Fever/drug therapy ; West Nile Fever/pathology ; West Nile Fever/virology ; West Nile virus/drug effects ; West Nile virus/genetics
Chemical Substances	Antiviral Agents ; RNA Replicase (EC 2.7.7.48) ; Tubercidin (M351LCX45Y) ; 7-deaza-2'-C-methyladenosine (QRR62J6WEF)
Language	English
Publishing date	2019-02-26
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	217602-6
ISSN	1098-6596 ; 0066-4804
ISSN (online)	1098-6596
ISSN	0066-4804
DOI	10.1128/AAC.02093-18
Database	MEDical Literature Analysis and Retrieval System OnLINE

In stock of ZB MED Cologne/Königswinter

Zs.A 809: Show issues			Location: Je nach Verfügbarkeit (siehe Angabe bei Bestand) bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular Jg. 1995 - 2021: Lesesall (1.OG) ab Jg. 2022: Lesesaal (EG)
Einzelsignatur: Show issues

Order via subito

Details ▾
- See ZB MED holdings
- Order with fees

Article: Diphyllin Shows a Broad-Spectrum Antiviral Activity against Multiple Medically Important Enveloped RNA and DNA Viruses

Štefánik, Michal / Bhosale, Dattatry Shivajirao / Haviernik, Jan / Straková, Petra / Fojtíková, Martina / Dufková, Lucie / Huvarová, Ivana / Salát, Jiří / Bartáček, Jan / Svoboda, Jan / Sedlák, Miloš / Růžek, Daniel / Miller, Andrew D. / Eyer, Luděk

Viruses. 2022 Feb. 09, v. 14, no. 2

2022

Abstract: Diphyllin is a natural arylnaphtalide lignan extracted from tropical plants of particular importance in traditional Chinese medicine. This compound has been described as a potent inhibitor of vacuolar (H⁺)ATPases and hence of the endosomal acidification ... ...

Abstract	Diphyllin is a natural arylnaphtalide lignan extracted from tropical plants of particular importance in traditional Chinese medicine. This compound has been described as a potent inhibitor of vacuolar (H⁺)ATPases and hence of the endosomal acidification process that is required by numerous enveloped viruses to trigger their respective viral infection cascades after entering host cells by receptor-mediated endocytosis. Accordingly, we report here a revised, updated, and improved synthesis of diphyllin, and demonstrate its antiviral activities against a panel of enveloped viruses from Flaviviridae, Phenuiviridae, Rhabdoviridae, and Herpesviridae families. Diphyllin is not cytotoxic for Vero and BHK-21 cells up to 100 µM and exerts a sub-micromolar or low-micromolar antiviral activity against tick-borne encephalitis virus, West Nile virus, Zika virus, Rift Valley fever virus, rabies virus, and herpes-simplex virus type 1. Our study shows that diphyllin is a broad-spectrum host cell-targeting antiviral agent that blocks the replication of multiple phylogenetically unrelated enveloped RNA and DNA viruses. In support of this, we also demonstrate that diphyllin is more than just a vacuolar (H⁺)ATPase inhibitor but may employ other antiviral mechanisms of action to inhibit the replication cycles of those viruses that do not enter host cells by endocytosis followed by low pH-dependent membrane fusion.
Keywords	DNA ; Oriental traditional medicine ; RNA ; Rabies lyssavirus ; Rift Valley fever phlebovirus ; Tick-borne encephalitis virus ; West Nile virus ; Zika virus ; acidification ; adenosinetriphosphatase ; antiviral agents ; antiviral properties ; cytotoxicity ; endocytosis ; herpes simplex ; lignans ; membrane fusion ; pH ; phylogeny ; vacuoles ; viruses
Language	English
Dates of publication	2022-0209
Publishing place	Multidisciplinary Digital Publishing Institute
Document type	Article
ZDB-ID	2516098-9
ISSN	1999-4915
ISSN	1999-4915
DOI	10.3390/v14020354
Database	NAL-Catalogue (AGRICOLA)

Order via subito

Article ; Online: Diphyllin Shows a Broad-Spectrum Antiviral Activity against Multiple Medically Important Enveloped RNA and DNA Viruses.

Viruses

2022 Volume 14, Issue 2

Abstract	Diphyllin is a natural arylnaphtalide lignan extracted from tropical plants of particular importance in traditional Chinese medicine. This compound has been described as a potent inhibitor of vacuolar (H
MeSH term(s)	Animals ; Antigens, Viral/metabolism ; Antiviral Agents/chemical synthesis ; Antiviral Agents/pharmacology ; Cell Line ; Cell Survival/drug effects ; Glucosides/pharmacology ; Lignans/chemical synthesis ; Lignans/pharmacology ; Vacuolar Proton-Translocating ATPases/antagonists & inhibitors ; Virus Replication/drug effects ; Viruses/classification ; Viruses/drug effects ; Viruses/metabolism
Chemical Substances	Antigens, Viral ; Antiviral Agents ; Glucosides ; Lignans ; cleistanthin B (30021-77-3) ; Vacuolar Proton-Translocating ATPases (EC 3.6.1.-) ; diphyllin (W4PN5LDP26)
Language	English
Publishing date	2022-02-09
Publishing country	Switzerland
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2516098-9
ISSN	1999-4915 ; 1999-4915
ISSN (online)	1999-4915
ISSN	1999-4915
DOI	10.3390/v14020354
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Article: FDA-Approved Drugs Efavirenz, Tipranavir, and Dasabuvir Inhibit Replication of Multiple Flaviviruses in Vero Cells.

Stefanik, Michal / Valdes, James J / Ezebuo, Fortunatus C / Haviernik, Jan / Uzochukwu, Ikemefuna C / Fojtikova, Martina / Salat, Jiri / Eyer, Ludek / Ruzek, Daniel

Microorganisms

2020 Volume 8, Issue 4

Abstract: Vector-borne flaviviruses (VBFs) affect human health worldwide, but no approved drugs are available specifically to treat VBF-associated infections. Here, we performed in silico screening of a library of U.S. Food and Drug Administration-approved ... ...

Abstract	Vector-borne flaviviruses (VBFs) affect human health worldwide, but no approved drugs are available specifically to treat VBF-associated infections. Here, we performed in silico screening of a library of U.S. Food and Drug Administration-approved antiviral drugs for their interaction with Zika virus proteins. Twelve hit drugs were identified by the docking experiments and tested in cell-based antiviral assay systems. Efavirenz, tipranavir, and dasabuvir at micromolar concentrations were identified to inhibit all VBFs tested; i.e., two representatives of mosquito-borne flaviviruses (Zika and West Nile viruses) and one representative of flaviviruses transmitted by ticks (tick-borne encephalitis virus). The results warrant further research into these drugs, either individually or in combination, as possible pan-flavivirus inhibitors.
Language	English
Publishing date	2020-04-20
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2720891-6
ISSN	2076-2607
ISSN	2076-2607
DOI	10.3390/microorganisms8040599
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Article: FDA-Approved Drugs Efavirenz, Tipranavir, and Dasabuvir Inhibit Replication of Multiple Flaviviruses in Vero Cells

Stefanik, Michal / Valdes, James J. / Ezebuo, Fortunatus C. / Haviernik, Jan / Uzochukwu, Ikemefuna C. / Fojtikova, Martina / Salat, Jiri / Eyer, Ludek / Ruzek, Daniel

Microorganisms. 2020 Apr. 20, v. 8, no. 4

2020

Abstract	Vector-borne flaviviruses (VBFs) affect human health worldwide, but no approved drugs are available specifically to treat VBF-associated infections. Here, we performed in silico screening of a library of U.S. Food and Drug Administration-approved antiviral drugs for their interaction with Zika virus proteins. Twelve hit drugs were identified by the docking experiments and tested in cell-based antiviral assay systems. Efavirenz, tipranavir, and dasabuvir at micromolar concentrations were identified to inhibit all VBFs tested; i.e., two representatives of mosquito-borne flaviviruses (Zika and West Nile viruses) and one representative of flaviviruses transmitted by ticks (tick-borne encephalitis virus). The results warrant further research into these drugs, either individually or in combination, as possible pan-flavivirus inhibitors.
Keywords	Tick-borne encephalitis virus ; Zika virus ; computer simulation ; human health
Language	English
Dates of publication	2020-0420
Publishing place	Multidisciplinary Digital Publishing Institute
Document type	Article
ZDB-ID	2720891-6
ISSN	2076-2607
ISSN	2076-2607
DOI	10.3390/microorganisms8040599
Database	NAL-Catalogue (AGRICOLA)

Order via subito

Article ; Online: Arbidol (Umifenovir): A Broad-Spectrum Antiviral Drug That Inhibits Medically Important Arthropod-Borne Flaviviruses.

Haviernik, Jan / Štefánik, Michal / Fojtíková, Martina / Kali, Sabrina / Tordo, Noël / Rudolf, Ivo / Hubálek, Zdeněk / Eyer, Luděk / Ruzek, Daniel

Viruses

2018 Volume 10, Issue 4

Abstract: Arthropod-borne flaviviruses are human pathogens of global medical importance, against which no effective small molecule-based antiviral therapy has currently been reported. Arbidol (umifenovir) is a broad-spectrum antiviral compound approved in Russia ... ...

Abstract	Arthropod-borne flaviviruses are human pathogens of global medical importance, against which no effective small molecule-based antiviral therapy has currently been reported. Arbidol (umifenovir) is a broad-spectrum antiviral compound approved in Russia and China for prophylaxis and treatment of influenza. This compound shows activities against numerous DNA and RNA viruses. The mode of action is based predominantly on impairment of critical steps in virus-cell interactions. Here we demonstrate that arbidol possesses micromolar-level anti-viral effects (EC
MeSH term(s)	Animals ; Antiviral Agents/pharmacology ; Antiviral Agents/toxicity ; Arthropod Vectors/virology ; Cell Line ; Cell Survival/drug effects ; Chlorocebus aethiops ; Encephalitis Viruses, Tick-Borne/drug effects ; Encephalitis Viruses, Tick-Borne/genetics ; Flavivirus/drug effects ; Flavivirus/genetics ; Flavivirus Infections/virology ; Gene Expression Regulation, Viral/drug effects ; Humans ; Indoles/pharmacology ; Indoles/toxicity ; Inhibitory Concentration 50 ; Vero Cells ; Viral Envelope Proteins/genetics ; West Nile virus/drug effects ; West Nile virus/genetics ; Zika Virus/drug effects ; Zika Virus/genetics
Chemical Substances	Antiviral Agents ; Indoles ; Viral Envelope Proteins ; umifenovir (93M09WW4RU)
Keywords	covid19
Language	English
Publishing date	2018-04-10
Publishing country	Switzerland
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2516098-9
ISSN	1999-4915 ; 1999-4915
ISSN (online)	1999-4915
ISSN	1999-4915
DOI	10.3390/v10040184
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Article ; Online: Preparation of nanoliposomes by microfluidic mixing in herring-bone channel and the role of membrane fluidity in liposomes formation.

Kotouček, Jan / Hubatka, František / Mašek, Josef / Kulich, Pavel / Velínská, Kamila / Bezděková, Jaroslava / Fojtíková, Martina / Bartheldyová, Eliška / Tomečková, Andrea / Stráská, Jana / Hrebík, Dominik / Macaulay, Stuart / Kratochvílová, Irena / Raška, Milan / Turánek, Jaroslav

Scientific reports

2020 Volume 10, Issue 1, Page(s) 5595

Abstract: Introduction of microfluidic mixing technique opens a new door for preparation of the liposomes and lipid-based nanoparticles by on-chip technologies that are applicable in a laboratory and industrial scale. This study demonstrates the role of ... ...

Abstract	Introduction of microfluidic mixing technique opens a new door for preparation of the liposomes and lipid-based nanoparticles by on-chip technologies that are applicable in a laboratory and industrial scale. This study demonstrates the role of phospholipid bilayer fragment as the key intermediate in the mechanism of liposome formation by microfluidic mixing in the channel with "herring-bone" geometry used with the instrument NanoAssemblr. The fluidity of the lipid bilayer expressed as fluorescence anisotropy of the probe N,N,N-Trimethyl-4-(6-phenyl-1,3,5-hexatrien-1-yl) was found to be the basic parameter affecting the final size of formed liposomes prepared by microfluidic mixing of an ethanol solution of lipids and water phase. Both saturated and unsaturated lipids together with various content of cholesterol were used for liposome preparation and it was demonstrated, that an increase in fluidity results in a decrease of liposome size as analyzed by DLS. Gadolinium chelating lipids were used to visualize the fine structure of liposomes and bilayer fragments by CryoTEM. Experimental data and theoretical calculations are in good accordance with the theory of lipid disc micelle vesiculation.
MeSH term(s)	Biocompatible Materials/metabolism ; Cholestyramine Resin/metabolism ; Fluorescence Polarization ; Lab-On-A-Chip Devices ; Liposomes/chemical synthesis ; Membrane Fluidity ; Microfluidics/instrumentation ; Microfluidics/methods ; Nanostructures
Chemical Substances	Biocompatible Materials ; Liposomes ; Cholestyramine Resin (11041-12-6)
Language	English
Publishing date	2020-03-27
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2615211-3
ISSN	2045-2322 ; 2045-2322
ISSN (online)	2045-2322
ISSN	2045-2322
DOI	10.1038/s41598-020-62500-2
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Article ; Online: Antiviral activity of the adenosine analogue BCX4430 against West Nile virus and tick-borne flaviviruses.

Eyer, Luděk / Zouharová, Darina / Širmarová, Jana / Fojtíková, Martina / Štefánik, Michal / Haviernik, Jan / Nencka, Radim / de Clercq, Erik / Růžek, Daniel

Antiviral research

2017 Volume 142, Page(s) 63–67

Abstract: There are currently no approved antiviral therapies against medically important human flaviviruses. The imino-C-nucleoside BCX4430 shows broad-spectrum antiviral activity against a wide range of RNA viruses. Here, we demonstrate that BCX4430 inhibits ... ...

Abstract	There are currently no approved antiviral therapies against medically important human flaviviruses. The imino-C-nucleoside BCX4430 shows broad-spectrum antiviral activity against a wide range of RNA viruses. Here, we demonstrate that BCX4430 inhibits tick-borne species of the genus Flavivirus; however, the antiviral effect varies against individual species. Micro-molar BCX4430 levels inhibited tick-borne encephalitis virus (TBEV); while, approximately 3-8-fold higher concentrations were needed to inhibit louping ill virus and Kyasanur Forest disease virus. Moreover, the compound strongly inhibited in vitro replication of West Nile virus, a typical mosquito-transmitted flavivirus. Two chemical forms of the compound, i.e. BCX4430 and BCX4430 hydrochloride, were compared and both exerted similar inhibitory profiles in our in vitro antiviral assay systems and no or negligible cytotoxicity in porcine kidney stable and Vero cells. The obtained data indicate that, in addition to mosquito-borne flaviviruses, the compound has strong antiviral activity against members of the TBEV serocomplex.
MeSH term(s)	Adenosine/analogs & derivatives ; Adenosine/pharmacology ; Animals ; Antiviral Agents/pharmacology ; Cell Line ; Cell Survival/drug effects ; Chlorocebus aethiops ; Culicidae/virology ; Dose-Response Relationship, Drug ; Encephalitis Viruses, Tick-Borne/drug effects ; Encephalitis Viruses, Tick-Borne/pathogenicity ; Encephalitis, Tick-Borne/transmission ; Encephalitis, Tick-Borne/virology ; Flavivirus/drug effects ; Purine Nucleosides/antagonists & inhibitors ; Purine Nucleosides/chemistry ; Swine ; Tick-Borne Diseases ; Ticks/virology ; Vero Cells ; Virus Replication/drug effects ; West Nile virus/drug effects ; West Nile virus/pathogenicity
Chemical Substances	Antiviral Agents ; Purine Nucleosides ; Adenosine (K72T3FS567) ; immucillin A (OLF97F86A7)
Language	English
Publishing date	2017-03-21
Publishing country	Netherlands
Document type	Journal Article
ZDB-ID	306628-9
ISSN	1872-9096 ; 0166-3542
ISSN (online)	1872-9096
ISSN	0166-3542
DOI	10.1016/j.antiviral.2017.03.012
Database	MEDical Literature Analysis and Retrieval System OnLINE

Full text online

Accessible to users with ZB MED library card

In stock of ZB MED Cologne/Königswinter

Zs.A 1627: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
Jg. 1995 - 2021: Lesesall (1.OG)
ab Jg. 2022: Lesesaal (EG)

Order via subito

Details ▾

To top

More links

Kategorien

Order via subito

More links

Kategorien

Order via subito

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

More links

Kategorien

Order via subito

More links

Kategorien

Order via subito

More links

Kategorien

Order via subito

More links

Kategorien

Order via subito

More links

Kategorien

Order via subito

More links

Kategorien

Order via subito

Full text online

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito